Skip to main content

Measurement of retinal blood vessel width using computerized image analysis.

Publication ,  Journal Article
Eaton, AM; Hatchell, DL
Published in: Invest Ophthalmol Vis Sci
August 1988

We report a new method for quantitating retinal blood vessel width from fluorescein angiogram negatives using a computerized image analyser. Interuser and intrauser variability were 3 to 4 times lower than reported with older methods. In addition, model blood vessels were used to test the accuracy of our method under four clinically relevant conditions: variations in vessel width, fluorescein concentration, flash intensity and background fluorescence. Although computed measurements were affected by vessel width, fluorescein concentration and flash intensity, the linearity of the actual versus computed width was maintained during wide variations in all four conditions (r = 0.95, P less than 0.0001). Accuracy was best achieved at a flash intensity of 150 W/sec and a fluorescein concentration of 45 micrograms/ml. The results of this study provide a better understanding of factors affecting the apparent width of retinal blood vessels in fluorescein angiograms. This technique should be useful in rapidly obtaining accurate measurements of blood vessel width from fluorescein angiograms.

Duke Scholars

Published In

Invest Ophthalmol Vis Sci

ISSN

0146-0404

Publication Date

August 1988

Volume

29

Issue

8

Start / End Page

1258 / 1264

Location

United States

Related Subject Headings

  • Retinal Vessels
  • Osmolar Concentration
  • Ophthalmology & Optometry
  • Models, Cardiovascular
  • Light
  • Image Processing, Computer-Assisted
  • Fluorescein Angiography
  • Cats
  • Animals
  • 3212 Ophthalmology and optometry
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Eaton, A. M., & Hatchell, D. L. (1988). Measurement of retinal blood vessel width using computerized image analysis. Invest Ophthalmol Vis Sci, 29(8), 1258–1264.
Eaton, A. M., and D. L. Hatchell. “Measurement of retinal blood vessel width using computerized image analysis.Invest Ophthalmol Vis Sci 29, no. 8 (August 1988): 1258–64.
Eaton AM, Hatchell DL. Measurement of retinal blood vessel width using computerized image analysis. Invest Ophthalmol Vis Sci. 1988 Aug;29(8):1258–64.
Eaton, A. M., and D. L. Hatchell. “Measurement of retinal blood vessel width using computerized image analysis.Invest Ophthalmol Vis Sci, vol. 29, no. 8, Aug. 1988, pp. 1258–64.
Eaton AM, Hatchell DL. Measurement of retinal blood vessel width using computerized image analysis. Invest Ophthalmol Vis Sci. 1988 Aug;29(8):1258–1264.

Published In

Invest Ophthalmol Vis Sci

ISSN

0146-0404

Publication Date

August 1988

Volume

29

Issue

8

Start / End Page

1258 / 1264

Location

United States

Related Subject Headings

  • Retinal Vessels
  • Osmolar Concentration
  • Ophthalmology & Optometry
  • Models, Cardiovascular
  • Light
  • Image Processing, Computer-Assisted
  • Fluorescein Angiography
  • Cats
  • Animals
  • 3212 Ophthalmology and optometry