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Role of neutrophils in breakdown of the blood-retinal barrier following intravitreal injection of platelet-activating factor.

Publication ,  Journal Article
Smith, D; Lee, EK; Saloupis, P; Davis, JK; Hatchell, DL
Published in: Exp Eye Res
October 1994

Breakdown of the blood-retinal barrier occurs in inflammatory conditions and in ischemic retinal diseases such as diabetic retinopathy. Platelet-activating factor (PAF) is a potent inflammatory mediator which increases vascular permeability. The purpose of this study was to determine if intravitreally-injected PAF would cause breakdown of the blood-retinal barrier and, if so, by what mechanism. Fluorescein angiography was performed before and at 0.5, 1, 2, 3 and 4 hr after PAF injection into the vitreous cavity of rabbit eyes and the eyes were enucleated immediately for light and electron microscopy. Slow flowing thrombi were observed in all PAF-injected eyes. Complete vascular occlusion was observed in 10 of 16 eyes after 3 and 4 hr. There was no fluorescein leakage in any of eyes before or at 0.5 or 1 hr after PAF injection. Fourteen of 20 eyes had fluorescein leakage at 2, 3 and 4 hr after PAF injection. The extent of fluorescein leakage correlated with the degree of polymorphonuclear leukocyte (PMN) margination, disruption of the endothelial cell layer, infiltration into vascular walls and migration into the vitreous cavity. PMNs appeared to migrate by both intercellular and transcellular routes across the endothelium. Pretreatment of rabbits with a PAF inhibitor, BN52021, prevented most of the abnormal findings.

Duke Scholars

Published In

Exp Eye Res

DOI

ISSN

0014-4835

Publication Date

October 1994

Volume

59

Issue

4

Start / End Page

425 / 432

Location

England

Related Subject Headings

  • Time Factors
  • Retinal Vessels
  • Rabbits
  • Platelet Activating Factor
  • Ophthalmology & Optometry
  • Neutrophils
  • Neutrophil Activation
  • Microscopy, Electron
  • Fluorescein Angiography
  • Capillary Permeability
 

Citation

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Smith, D., Lee, E. K., Saloupis, P., Davis, J. K., & Hatchell, D. L. (1994). Role of neutrophils in breakdown of the blood-retinal barrier following intravitreal injection of platelet-activating factor. Exp Eye Res, 59(4), 425–432. https://doi.org/10.1006/exer.1994.1127
Smith, D., E. K. Lee, P. Saloupis, J. K. Davis, and D. L. Hatchell. “Role of neutrophils in breakdown of the blood-retinal barrier following intravitreal injection of platelet-activating factor.Exp Eye Res 59, no. 4 (October 1994): 425–32. https://doi.org/10.1006/exer.1994.1127.
Smith D, Lee EK, Saloupis P, Davis JK, Hatchell DL. Role of neutrophils in breakdown of the blood-retinal barrier following intravitreal injection of platelet-activating factor. Exp Eye Res. 1994 Oct;59(4):425–32.
Smith, D., et al. “Role of neutrophils in breakdown of the blood-retinal barrier following intravitreal injection of platelet-activating factor.Exp Eye Res, vol. 59, no. 4, Oct. 1994, pp. 425–32. Pubmed, doi:10.1006/exer.1994.1127.
Smith D, Lee EK, Saloupis P, Davis JK, Hatchell DL. Role of neutrophils in breakdown of the blood-retinal barrier following intravitreal injection of platelet-activating factor. Exp Eye Res. 1994 Oct;59(4):425–432.
Journal cover image

Published In

Exp Eye Res

DOI

ISSN

0014-4835

Publication Date

October 1994

Volume

59

Issue

4

Start / End Page

425 / 432

Location

England

Related Subject Headings

  • Time Factors
  • Retinal Vessels
  • Rabbits
  • Platelet Activating Factor
  • Ophthalmology & Optometry
  • Neutrophils
  • Neutrophil Activation
  • Microscopy, Electron
  • Fluorescein Angiography
  • Capillary Permeability