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Direct assessment of articular cartilage and underlying subchondral bone reveals a progressive gene expression change in human osteoarthritic knees.

Publication ,  Journal Article
Chou, C-H; Lee, C-H; Lu, L-S; Song, I-W; Chuang, H-P; Kuo, S-Y; Wu, J-Y; Chen, Y-T; Kraus, VB; Wu, C-C; Lee, MTM
Published in: Osteoarthritis Cartilage
March 2013

OBJECTIVE: To evaluate the interaction of articular cartilage (AC) and subchondral bone (SB) through analysis of osteoarthritis (OA)-related genes of site-matched tissue. DESIGN: We developed a novel method for isolating site-matched overlying AC and underlying SB from three and four regions of interest respectively from the human knee tibial plateau (n = 50). For each site, the severity of cartilage changes of OA were assessed histologically, and the severity of bone abnormalities were assessed by microcomputed tomography. An RNA isolation procedure was optimized that yielded high quality RNA from site-matched AC and SB tibial regions. Quantitative polymerase chain reaction (Q-PCR) analysis was performed to evaluate gene expression of 61 OA-associated genes for correlation with cartilage integrity and bone structure parameters. RESULTS: A total of 27 (44%) genes were coordinately up- or down-regulated in both tissues. The expression levels of 19 genes were statistically significantly correlated with the severity of AC degeneration and changes of SB structure; these included: ADAMTS1, ASPN, BMP6, BMPER, CCL2, CCL8, COL5A1, COL6A3, COL7A1, COL16A1, FRZB, GDF10, MMP3, OGN, OMD, POSTN, PTGES, TNFSF11 and WNT1. CONCLUSIONS: These results provide a strategy for identifying targets whose modification may have the potential to ameliorate pathological alterations and progression of disease in both AC and SB simultaneously. In addition, this is the first study, to our knowledge, to overcome the major difficulties related to isolation of high quality RNA from site-matched joint tissues. We expect this method to facilitate advances in our understanding of the coordinated molecular responses of the whole joint organ.

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Published In

Osteoarthritis Cartilage

DOI

EISSN

1522-9653

Publication Date

March 2013

Volume

21

Issue

3

Start / End Page

450 / 461

Location

England

Related Subject Headings

  • X-Ray Microtomography
  • Tibia
  • RNA
  • Polymerase Chain Reaction
  • Osteoarthritis, Knee
  • Middle Aged
  • Male
  • Knee Joint
  • Humans
  • Gene Expression
 

Citation

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Chou, C.-H., Lee, C.-H., Lu, L.-S., Song, I.-W., Chuang, H.-P., Kuo, S.-Y., … Lee, M. T. M. (2013). Direct assessment of articular cartilage and underlying subchondral bone reveals a progressive gene expression change in human osteoarthritic knees. Osteoarthritis Cartilage, 21(3), 450–461. https://doi.org/10.1016/j.joca.2012.11.016
Chou, C. -. H., C. -. H. Lee, L. -. S. Lu, I. -. W. Song, H. -. P. Chuang, S. -. Y. Kuo, J. -. Y. Wu, et al. “Direct assessment of articular cartilage and underlying subchondral bone reveals a progressive gene expression change in human osteoarthritic knees.Osteoarthritis Cartilage 21, no. 3 (March 2013): 450–61. https://doi.org/10.1016/j.joca.2012.11.016.
Chou C-H, Lee C-H, Lu L-S, Song I-W, Chuang H-P, Kuo S-Y, et al. Direct assessment of articular cartilage and underlying subchondral bone reveals a progressive gene expression change in human osteoarthritic knees. Osteoarthritis Cartilage. 2013 Mar;21(3):450–61.
Chou, C. .. H., et al. “Direct assessment of articular cartilage and underlying subchondral bone reveals a progressive gene expression change in human osteoarthritic knees.Osteoarthritis Cartilage, vol. 21, no. 3, Mar. 2013, pp. 450–61. Pubmed, doi:10.1016/j.joca.2012.11.016.
Chou C-H, Lee C-H, Lu L-S, Song I-W, Chuang H-P, Kuo S-Y, Wu J-Y, Chen Y-T, Kraus VB, Wu C-C, Lee MTM. Direct assessment of articular cartilage and underlying subchondral bone reveals a progressive gene expression change in human osteoarthritic knees. Osteoarthritis Cartilage. 2013 Mar;21(3):450–461.
Journal cover image

Published In

Osteoarthritis Cartilage

DOI

EISSN

1522-9653

Publication Date

March 2013

Volume

21

Issue

3

Start / End Page

450 / 461

Location

England

Related Subject Headings

  • X-Ray Microtomography
  • Tibia
  • RNA
  • Polymerase Chain Reaction
  • Osteoarthritis, Knee
  • Middle Aged
  • Male
  • Knee Joint
  • Humans
  • Gene Expression