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Use of peptide nucleic acid-fluorescence in situ hybridization for definitive, rapid identification of five common Candida species.

Publication ,  Journal Article
Reller, ME; Mallonee, AB; Kwiatkowski, NP; Merz, WG
Published in: J Clin Microbiol
November 2007

We investigated a 2.5-h peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) assay with five Candida species-specific probes to identify Candida colonies and compared it to standard 2-h to 5-day phenotypic identification methods. Suspensions were made and slides were prepared and read for fluorescence per the manufacturer's instructions. Sensitivity was 99% (109/110), and specificity was 99% (129/130). PNA-FISH can rapidly identify those Candida species isolated most frequently.

Duke Scholars

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

November 2007

Volume

45

Issue

11

Start / End Page

3802 / 3803

Location

United States

Related Subject Headings

  • Peptide Nucleic Acids
  • Nucleic Acid Probes
  • Microbiology
  • In Situ Hybridization, Fluorescence
  • Humans
  • Candida
  • 3207 Medical microbiology
  • 3202 Clinical sciences
  • 11 Medical and Health Sciences
  • 07 Agricultural and Veterinary Sciences
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Reller, M. E., Mallonee, A. B., Kwiatkowski, N. P., & Merz, W. G. (2007). Use of peptide nucleic acid-fluorescence in situ hybridization for definitive, rapid identification of five common Candida species. J Clin Microbiol, 45(11), 3802–3803. https://doi.org/10.1128/JCM.01127-07
Reller, Megan E., Amanda B. Mallonee, Nicole P. Kwiatkowski, and William G. Merz. “Use of peptide nucleic acid-fluorescence in situ hybridization for definitive, rapid identification of five common Candida species.J Clin Microbiol 45, no. 11 (November 2007): 3802–3. https://doi.org/10.1128/JCM.01127-07.
Reller ME, Mallonee AB, Kwiatkowski NP, Merz WG. Use of peptide nucleic acid-fluorescence in situ hybridization for definitive, rapid identification of five common Candida species. J Clin Microbiol. 2007 Nov;45(11):3802–3.
Reller, Megan E., et al. “Use of peptide nucleic acid-fluorescence in situ hybridization for definitive, rapid identification of five common Candida species.J Clin Microbiol, vol. 45, no. 11, Nov. 2007, pp. 3802–03. Pubmed, doi:10.1128/JCM.01127-07.
Reller ME, Mallonee AB, Kwiatkowski NP, Merz WG. Use of peptide nucleic acid-fluorescence in situ hybridization for definitive, rapid identification of five common Candida species. J Clin Microbiol. 2007 Nov;45(11):3802–3803.

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

November 2007

Volume

45

Issue

11

Start / End Page

3802 / 3803

Location

United States

Related Subject Headings

  • Peptide Nucleic Acids
  • Nucleic Acid Probes
  • Microbiology
  • In Situ Hybridization, Fluorescence
  • Humans
  • Candida
  • 3207 Medical microbiology
  • 3202 Clinical sciences
  • 11 Medical and Health Sciences
  • 07 Agricultural and Veterinary Sciences