Detection of phosphorylation by enzymatic techniques.
Publication
, Journal Article
Shenolikar, S
Published in: Curr Protoc Protein Sci
May 2001
This unit presents protocols for examining the functional effects elicited by nonspecific acid or alkaline phosphatases that dephosphorylate many phosphoproteins in vitro. Additional protocols describe digestion of phosphoproteins with a protein serine/threonine phosphatase and protein tyrosine phosphatase. A support protocol has been included to identify the radiolabel as (32)P(I) based on its ability to form a complex with ammonium molybdate.
Duke Scholars
Published In
Curr Protoc Protein Sci
DOI
EISSN
1934-3663
Publication Date
May 2001
Volume
Chapter 13
Start / End Page
Unit13.5
Location
United States
Related Subject Headings
- Phosphorylation
- Phosphorus Radioisotopes
- Phosphoric Monoester Hydrolases
- Phosphoproteins
- Phosphoprotein Phosphatases
- Biochemistry
- Alkaline Phosphatase
- Acid Phosphatase
Citation
APA
Chicago
ICMJE
MLA
NLM
Shenolikar, S. (2001). Detection of phosphorylation by enzymatic techniques. Curr Protoc Protein Sci, Chapter 13, Unit13.5. https://doi.org/10.1002/0471140864.ps1305s05
Shenolikar, S. “Detection of phosphorylation by enzymatic techniques.” Curr Protoc Protein Sci Chapter 13 (May 2001): Unit13.5. https://doi.org/10.1002/0471140864.ps1305s05.
Shenolikar S. Detection of phosphorylation by enzymatic techniques. Curr Protoc Protein Sci. 2001 May;Chapter 13:Unit13.5.
Shenolikar, S. “Detection of phosphorylation by enzymatic techniques.” Curr Protoc Protein Sci, vol. Chapter 13, May 2001, p. Unit13.5. Pubmed, doi:10.1002/0471140864.ps1305s05.
Shenolikar S. Detection of phosphorylation by enzymatic techniques. Curr Protoc Protein Sci. 2001 May;Chapter 13:Unit13.5.
Published In
Curr Protoc Protein Sci
DOI
EISSN
1934-3663
Publication Date
May 2001
Volume
Chapter 13
Start / End Page
Unit13.5
Location
United States
Related Subject Headings
- Phosphorylation
- Phosphorus Radioisotopes
- Phosphoric Monoester Hydrolases
- Phosphoproteins
- Phosphoprotein Phosphatases
- Biochemistry
- Alkaline Phosphatase
- Acid Phosphatase