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The products of gene I and the overlapping in-frame gene XI are required for filamentous phage assembly.

Publication ,  Journal Article
Rapoza, MP; Webster, RE
Published in: J Mol Biol
May 5, 1995

The class I filamentous bacteriophage are non-lytic single-stranded DNA phage, which are assembled at the cell envelope as they are extruded from the Gram-negative bacteria, Escherichia coli. The process requires the products of the phage genes I and IV, which reside in the inner and outer membrane, respectively, and are not present in the mature phage particle. Gene I encodes two proteins, the full length 348-residue pI and a smaller pI*, which this report shows is the result of an internal translation initiation event at methionine codon 241. Both pI and pI* are shown to be required for phage assembly. Therefore, pI* can be considered the product of an additional phage gene, XI, which is a separate in-frame gene that overlaps gene I. Both proteins contain a 13-residue region adjacent to the cytoplasmic face of the inner membrane that probably exists as a positively charged amphiphilic helix. Although this region is not required for membrane insertion of pI and pI*, it is shown to be required for phage assembly. Oligonucleotide-directed mutagenesis of this region, which removes positive charges or alters the hydrophobic face of the putative helix, renders pI and pI* unable to function in phage assembly. This region of pI and pI* is highly homologous in structure to the carboxyl-terminal 11 amino acids of pVIII, the main coat protein, which also reside adjacent to the cytoplasmic face of the inner membrane.

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Published In

J Mol Biol

DOI

ISSN

0022-2836

Publication Date

May 5, 1995

Volume

248

Issue

3

Start / End Page

627 / 638

Location

Netherlands

Related Subject Headings

  • Virus Replication
  • Viral Proteins
  • Recombinant Fusion Proteins
  • Protein Structure, Secondary
  • Protein Biosynthesis
  • Mutagenesis, Site-Directed
  • Molecular Sequence Data
  • Genetic Complementation Test
  • Genes, Viral
  • Genes, Overlapping
 

Citation

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Rapoza, M. P., & Webster, R. E. (1995). The products of gene I and the overlapping in-frame gene XI are required for filamentous phage assembly. J Mol Biol, 248(3), 627–638. https://doi.org/10.1006/jmbi.1995.0247
Rapoza, M. P., and R. E. Webster. “The products of gene I and the overlapping in-frame gene XI are required for filamentous phage assembly.J Mol Biol 248, no. 3 (May 5, 1995): 627–38. https://doi.org/10.1006/jmbi.1995.0247.
Rapoza, M. P., and R. E. Webster. “The products of gene I and the overlapping in-frame gene XI are required for filamentous phage assembly.J Mol Biol, vol. 248, no. 3, May 1995, pp. 627–38. Pubmed, doi:10.1006/jmbi.1995.0247.
Journal cover image

Published In

J Mol Biol

DOI

ISSN

0022-2836

Publication Date

May 5, 1995

Volume

248

Issue

3

Start / End Page

627 / 638

Location

Netherlands

Related Subject Headings

  • Virus Replication
  • Viral Proteins
  • Recombinant Fusion Proteins
  • Protein Structure, Secondary
  • Protein Biosynthesis
  • Mutagenesis, Site-Directed
  • Molecular Sequence Data
  • Genetic Complementation Test
  • Genes, Viral
  • Genes, Overlapping