Imaging spatiotemporal dynamics of neuronal signaling using fluorescence resonance energy transfer and fluorescence lifetime imaging microscopy.
The spatiotemporal localization of neuronal signaling is important for triggering neuronal responses in specific locations at precise times. Fluorescence resonance energy transfer imaging enables measurement of spatiotemporal dynamics of signaling activity in live neurons. Although the usefulness of fluorescence resonance energy transfer is well recognized, there are many difficulties in applying it, particularly when imaging in neuronal micro-compartments in light-scattering brain tissue. Fluorescence resonance energy transfer has been imaged using several techniques including intensity-based methods, fluorescence lifetime imaging and fluorescence anisotropy imaging. These methods have different advantages and disadvantages, and thus are suitable in different applications.
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Related Subject Headings
- Signal Transduction
- Neurons
- Microscopy, Fluorescence
- Humans
- Fluorescence Resonance Energy Transfer
- Animals
- 3209 Neurosciences
- 1702 Cognitive Sciences
- 1109 Neurosciences
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Signal Transduction
- Neurons
- Microscopy, Fluorescence
- Humans
- Fluorescence Resonance Energy Transfer
- Animals
- 3209 Neurosciences
- 1702 Cognitive Sciences
- 1109 Neurosciences