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Deficient adenylate cyclase regulatory protein in renal membranes from a patient with pseudohypoparathyroidism.

Publication ,  Journal Article
Downs, RW; Levine, MA; Drezner, MK; Burch, WM; Spiegel, AM
Published in: J Clin Invest
February 1983

Recent studies have established that some patients with pseudohypoparathyroidism have a deficiency of the adenylate cyclase regulatory protein (the G unit) in plasma membranes from erythrocytes, platelets, and fibroblasts. We have directly measured the activity of the G unit in renal membranes from a patient with pseudohypoparathyroidism who, in addition to parathyroid hormone resistance, has resistance to thyrotropin and gonadotropins. Erythrocyte membrane G unit activity was 57% that of control erythrocyte membranes. Lubrol PX extracts of renal membranes had only 30% of the G unit activity of control renal membrane extracts, whether assayed with sodium fluoride or guanosine-5'-O-(3-thiotriphosphate) (GTP-gamma-S). In cholate extracts, the G unit activity was 37 and 48% of control with fluoride or GTP-gamma-S, respectively. Cholera toxin-dependent incorporation of [32P]ADP-ribose into the 42,000-Mr subunit of the G unit was decreased in renal membranes from the patient compared with control renal membranes. The data demonstrate that the membrane G unit deficiency in pseudohypoparathyroidism extends to the cells of a clinically relevant parathyroid hormone target tissue.

Duke Scholars

Published In

J Clin Invest

DOI

ISSN

0021-9738

Publication Date

February 1983

Volume

71

Issue

2

Start / End Page

231 / 235

Location

United States

Related Subject Headings

  • Pseudohypoparathyroidism
  • Molecular Weight
  • Kidney
  • Immunology
  • Humans
  • Female
  • Erythrocyte Membrane
  • Adolescent
  • Adenylyl Cyclases
  • 42 Health sciences
 

Citation

APA
Chicago
ICMJE
MLA
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Downs, R. W., Levine, M. A., Drezner, M. K., Burch, W. M., & Spiegel, A. M. (1983). Deficient adenylate cyclase regulatory protein in renal membranes from a patient with pseudohypoparathyroidism. J Clin Invest, 71(2), 231–235. https://doi.org/10.1172/jci110763
Downs, R. W., M. A. Levine, M. K. Drezner, W. M. Burch, and A. M. Spiegel. “Deficient adenylate cyclase regulatory protein in renal membranes from a patient with pseudohypoparathyroidism.J Clin Invest 71, no. 2 (February 1983): 231–35. https://doi.org/10.1172/jci110763.
Downs RW, Levine MA, Drezner MK, Burch WM, Spiegel AM. Deficient adenylate cyclase regulatory protein in renal membranes from a patient with pseudohypoparathyroidism. J Clin Invest. 1983 Feb;71(2):231–5.
Downs, R. W., et al. “Deficient adenylate cyclase regulatory protein in renal membranes from a patient with pseudohypoparathyroidism.J Clin Invest, vol. 71, no. 2, Feb. 1983, pp. 231–35. Pubmed, doi:10.1172/jci110763.
Downs RW, Levine MA, Drezner MK, Burch WM, Spiegel AM. Deficient adenylate cyclase regulatory protein in renal membranes from a patient with pseudohypoparathyroidism. J Clin Invest. 1983 Feb;71(2):231–235.

Published In

J Clin Invest

DOI

ISSN

0021-9738

Publication Date

February 1983

Volume

71

Issue

2

Start / End Page

231 / 235

Location

United States

Related Subject Headings

  • Pseudohypoparathyroidism
  • Molecular Weight
  • Kidney
  • Immunology
  • Humans
  • Female
  • Erythrocyte Membrane
  • Adolescent
  • Adenylyl Cyclases
  • 42 Health sciences