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Rapid and transient localization of the leader RNA of vesicular stomatitis virus in the nuclei of infected cells.

Publication ,  Journal Article
Kurilla, MG; Piwnica-Worms, H; Keene, JD
Published in: Proc Natl Acad Sci U S A
September 1982

The leader RNA transcript from the 3' end of the genome of vesicular stomatitis virus (VSV) has been detected in both the nucleus and cytoplasm of infected baby hamster kidney (BHK) cells. In the cytoplasm, leader RNA accumulated gradually throughout the infection to about 200 molecules per cell at 6 hr after infection. In the nucleus, however, there was a sharp and rapid increase in the concentration of leader RNA to approximately equal to 300 molecules per cell at about 2 hr after infection that decreased rapidly by 3 hr. This report presents evidence for nuclear localization of transcription products of a (-)-strand RNA virus other than influenza and supports the hypothesis that the leader RNA plays a role in the shutoff of host cell transcription.

Duke Scholars

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

September 1982

Volume

79

Issue

17

Start / End Page

5240 / 5244

Location

United States

Related Subject Headings

  • Vesicular stomatitis Indiana virus
  • Transcription, Genetic
  • RNA, Viral
  • Nucleic Acid Hybridization
  • Kidney
  • Cytoplasm
  • Cricetinae
  • Cell Transformation, Viral
  • Cell Nucleus
  • Cell Line
 

Citation

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ICMJE
MLA
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Kurilla, M. G., Piwnica-Worms, H., & Keene, J. D. (1982). Rapid and transient localization of the leader RNA of vesicular stomatitis virus in the nuclei of infected cells. Proc Natl Acad Sci U S A, 79(17), 5240–5244. https://doi.org/10.1073/pnas.79.17.5240
Kurilla, M. G., H. Piwnica-Worms, and J. D. Keene. “Rapid and transient localization of the leader RNA of vesicular stomatitis virus in the nuclei of infected cells.Proc Natl Acad Sci U S A 79, no. 17 (September 1982): 5240–44. https://doi.org/10.1073/pnas.79.17.5240.
Kurilla MG, Piwnica-Worms H, Keene JD. Rapid and transient localization of the leader RNA of vesicular stomatitis virus in the nuclei of infected cells. Proc Natl Acad Sci U S A. 1982 Sep;79(17):5240–4.
Kurilla, M. G., et al. “Rapid and transient localization of the leader RNA of vesicular stomatitis virus in the nuclei of infected cells.Proc Natl Acad Sci U S A, vol. 79, no. 17, Sept. 1982, pp. 5240–44. Pubmed, doi:10.1073/pnas.79.17.5240.
Kurilla MG, Piwnica-Worms H, Keene JD. Rapid and transient localization of the leader RNA of vesicular stomatitis virus in the nuclei of infected cells. Proc Natl Acad Sci U S A. 1982 Sep;79(17):5240–5244.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

September 1982

Volume

79

Issue

17

Start / End Page

5240 / 5244

Location

United States

Related Subject Headings

  • Vesicular stomatitis Indiana virus
  • Transcription, Genetic
  • RNA, Viral
  • Nucleic Acid Hybridization
  • Kidney
  • Cytoplasm
  • Cricetinae
  • Cell Transformation, Viral
  • Cell Nucleus
  • Cell Line