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WE‐D‐330A‐02: Optical‐Computed‐and Emmission Tomography: Applications in Cancer Research

Publication ,  Conference
Oldham, M; Sakhalkar, H; Oliver, T; Dewhirst, M
Published in: Medical Physics
January 1, 2006

Purpose: This work explores the potential of optical‐computed and emission‐tomography (OCT/OET), when coupled with optical clearing techniques, for imaging aspects of biological structure and function for cancer research. OCT/OET are the optical analogues to x‐ray‐CT and SPECT respectively, and can yield high resolution high contrast 3D images of a variety of inherent or applied absorbing and fluorescing stains. Materials and Methods Several methods of staining tissue have been explored and applied to a range of tissue types. Xenograph tumor microvasculature was labeled with both passive absorbing stain and fluorescing active probes (e.g. lectin conjugated with FITC) by tail vein injection. Murine vasculature in major organs (lung, heart, brain) were stained with absorbing dye in a similar manner. OCT/OET imaging was performed using an in‐house custom built scanner. Isotropic 3D transmision and emission data were reconstructed using tomographic algorithms from equiangularly spaced projection images. Results: Isotropic high resolution 3D image data of the xenograph tumor showed extensive peripheral microvasculature with the occasional larger vessels penetrating to the tumor core. High‐quality 3D images of the lungs were achieved showing clear differences in perfusion between irradiated and unirradiated lung regions. Exquisite high contrast images were acquired of the vasculature and myocardial perfusion of the murine heart. Conclusion Primary advantages of OCT/OET include the preservation of tissue structure in 3D (tissue sectioning is not required), and the ability to acquire co‐registered images of both structure (e.g.micro‐vasculature) and function (e.g. perfusion, gene expression). Higher spatial resolution and higher contrast is achieved when compared with alternative modalities like micro‐CT and micro‐MRI. The techniques are versatile as imaging can be performed on a wide variety of absorbing and fluorescing stains. © 2006, American Association of Physicists in Medicine. All rights reserved.

Duke Scholars

Published In

Medical Physics

DOI

ISSN

0094-2405

Publication Date

January 1, 2006

Volume

33

Issue

6

Start / End Page

2239 / 2240

Related Subject Headings

  • Nuclear Medicine & Medical Imaging
  • 5105 Medical and biological physics
  • 4003 Biomedical engineering
  • 1112 Oncology and Carcinogenesis
  • 0903 Biomedical Engineering
  • 0299 Other Physical Sciences
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Oldham, M., Sakhalkar, H., Oliver, T., & Dewhirst, M. (2006). WE‐D‐330A‐02: Optical‐Computed‐and Emmission Tomography: Applications in Cancer Research. In Medical Physics (Vol. 33, pp. 2239–2240). https://doi.org/10.1118/1.2241740
Oldham, M., H. Sakhalkar, T. Oliver, and M. Dewhirst. “WE‐D‐330A‐02: Optical‐Computed‐and Emmission Tomography: Applications in Cancer Research.” In Medical Physics, 33:2239–40, 2006. https://doi.org/10.1118/1.2241740.
Oldham M, Sakhalkar H, Oliver T, Dewhirst M. WE‐D‐330A‐02: Optical‐Computed‐and Emmission Tomography: Applications in Cancer Research. In: Medical Physics. 2006. p. 2239–40.
Oldham, M., et al. “WE‐D‐330A‐02: Optical‐Computed‐and Emmission Tomography: Applications in Cancer Research.” Medical Physics, vol. 33, no. 6, 2006, pp. 2239–40. Scopus, doi:10.1118/1.2241740.
Oldham M, Sakhalkar H, Oliver T, Dewhirst M. WE‐D‐330A‐02: Optical‐Computed‐and Emmission Tomography: Applications in Cancer Research. Medical Physics. 2006. p. 2239–2240.

Published In

Medical Physics

DOI

ISSN

0094-2405

Publication Date

January 1, 2006

Volume

33

Issue

6

Start / End Page

2239 / 2240

Related Subject Headings

  • Nuclear Medicine & Medical Imaging
  • 5105 Medical and biological physics
  • 4003 Biomedical engineering
  • 1112 Oncology and Carcinogenesis
  • 0903 Biomedical Engineering
  • 0299 Other Physical Sciences