Analysis of bacteriophage T4 coat proteins by two‐dimensional electrophoresis and computerized densitometry
Publication
, Journal Article
Gersten, DM; Kurian, P; Ledly, G; Park, CM; Suhocki, PV
Published in: ELECTROPHORESIS
January 1, 1981
We have designed treatment conditions which allow analysis of the coat proteins of bacteriophage T4D+ by high resolution two‐dimensional electrophoresis. The best results were obtained when the mature phage particles were subjected to hypertoniclysis and DNase treatment before solubilization with sodium dodecyl sulfate (SDS). Samples containing 250 ug protein gave the optimum resolution, producing 45 spots as visualized by Coomassie Blue staining. Computerized densitometry measurements showed that these proteins range in relative abundance from less than 0.22% to 11.8% of the total. Copyright © 1981 Verlag Chemie, GmbH
Duke Scholars
Published In
ELECTROPHORESIS
DOI
EISSN
1522-2683
ISSN
0173-0835
Publication Date
January 1, 1981
Volume
2
Issue
2
Start / End Page
123 / 125
Related Subject Headings
- Analytical Chemistry
- 3401 Analytical chemistry
- 3101 Biochemistry and cell biology
- 0904 Chemical Engineering
- 0601 Biochemistry and Cell Biology
- 0301 Analytical Chemistry
Citation
APA
Chicago
ICMJE
MLA
NLM
Gersten, D. M., Kurian, P., Ledly, G., Park, C. M., & Suhocki, P. V. (1981). Analysis of bacteriophage T4 coat proteins by two‐dimensional electrophoresis and computerized densitometry. ELECTROPHORESIS, 2(2), 123–125. https://doi.org/10.1002/elps.1150020210
Gersten, D. M., P. Kurian, G. Ledly, C. M. Park, and P. V. Suhocki. “Analysis of bacteriophage T4 coat proteins by two‐dimensional electrophoresis and computerized densitometry.” ELECTROPHORESIS 2, no. 2 (January 1, 1981): 123–25. https://doi.org/10.1002/elps.1150020210.
Gersten DM, Kurian P, Ledly G, Park CM, Suhocki PV. Analysis of bacteriophage T4 coat proteins by two‐dimensional electrophoresis and computerized densitometry. ELECTROPHORESIS. 1981 Jan 1;2(2):123–5.
Gersten, D. M., et al. “Analysis of bacteriophage T4 coat proteins by two‐dimensional electrophoresis and computerized densitometry.” ELECTROPHORESIS, vol. 2, no. 2, Jan. 1981, pp. 123–25. Scopus, doi:10.1002/elps.1150020210.
Gersten DM, Kurian P, Ledly G, Park CM, Suhocki PV. Analysis of bacteriophage T4 coat proteins by two‐dimensional electrophoresis and computerized densitometry. ELECTROPHORESIS. 1981 Jan 1;2(2):123–125.
Published In
ELECTROPHORESIS
DOI
EISSN
1522-2683
ISSN
0173-0835
Publication Date
January 1, 1981
Volume
2
Issue
2
Start / End Page
123 / 125
Related Subject Headings
- Analytical Chemistry
- 3401 Analytical chemistry
- 3101 Biochemistry and cell biology
- 0904 Chemical Engineering
- 0601 Biochemistry and Cell Biology
- 0301 Analytical Chemistry