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Identification of wounding and topping responsive small RNAs in tobacco (Nicotiana tabacum).

Publication ,  Journal Article
Tang, S; Wang, Y; Li, Z; Gui, Y; Xiao, B; Xie, J; Zhu, Q-H; Fan, L
Published in: BMC Plant Biol
February 22, 2012

BACKGROUND: MicroRNAs (miRNAs) and short interfering RNAs (siRNAs) are two major classes of small RNAs. They play important regulatory roles in plants and animals by regulating transcription, stability and/or translation of target genes in a sequence-complementary dependent manner. Over 4,000 miRNAs and several classes of siRNAs have been identified in plants, but in tobacco only computational prediction has been performed and no tobacco-specific miRNA has been experimentally identified. Wounding is believed to induce defensive response in tobacco, but the mechanism responsible for this response is yet to be uncovered. RESULTS: To get insight into the role of small RNAs in damage-induced responses, we sequenced and analysed small RNA populations in roots and leaves from wounding or topping treated tobacco plants. In addition to confirmation of expression of 27 known miRNA families, we identified 59 novel tobacco-specific miRNA members of 38 families and a large number of loci generating phased 21- or 24-nt small RNAs (including ta-siRNAs). A number of miRNAs and phased small RNAs were found to be responsive to wounding or topping treatment. Targets of small RNAs were further surveyed by degradome sequencing. CONCLUSIONS: The expression changes of miRNAs and phased small RNAs responsive to wounding or topping and identification of defense related targets for these small RNAs suggest that the inducible defense response in tobacco might be controlled by pathways involving small RNAs.

Duke Scholars

Published In

BMC Plant Biol

DOI

EISSN

1471-2229

Publication Date

February 22, 2012

Volume

12

Start / End Page

28

Location

England

Related Subject Headings

  • RNA, Small Interfering
  • RNA, Plant
  • Plant Roots
  • Plant Leaves
  • Plant Biology & Botany
  • Nicotiana
  • MicroRNAs
  • 3108 Plant biology
  • 3004 Crop and pasture production
  • 0703 Crop and Pasture Production
 

Citation

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Tang, S., Wang, Y., Li, Z., Gui, Y., Xiao, B., Xie, J., … Fan, L. (2012). Identification of wounding and topping responsive small RNAs in tobacco (Nicotiana tabacum). BMC Plant Biol, 12, 28. https://doi.org/10.1186/1471-2229-12-28
Tang, She, Yu Wang, Zefeng Li, Yijie Gui, Bingguang Xiao, Jiahua Xie, Qian-Hao Zhu, and Longjiang Fan. “Identification of wounding and topping responsive small RNAs in tobacco (Nicotiana tabacum).BMC Plant Biol 12 (February 22, 2012): 28. https://doi.org/10.1186/1471-2229-12-28.
Tang S, Wang Y, Li Z, Gui Y, Xiao B, Xie J, et al. Identification of wounding and topping responsive small RNAs in tobacco (Nicotiana tabacum). BMC Plant Biol. 2012 Feb 22;12:28.
Tang, She, et al. “Identification of wounding and topping responsive small RNAs in tobacco (Nicotiana tabacum).BMC Plant Biol, vol. 12, Feb. 2012, p. 28. Pubmed, doi:10.1186/1471-2229-12-28.
Tang S, Wang Y, Li Z, Gui Y, Xiao B, Xie J, Zhu Q-H, Fan L. Identification of wounding and topping responsive small RNAs in tobacco (Nicotiana tabacum). BMC Plant Biol. 2012 Feb 22;12:28.
Journal cover image

Published In

BMC Plant Biol

DOI

EISSN

1471-2229

Publication Date

February 22, 2012

Volume

12

Start / End Page

28

Location

England

Related Subject Headings

  • RNA, Small Interfering
  • RNA, Plant
  • Plant Roots
  • Plant Leaves
  • Plant Biology & Botany
  • Nicotiana
  • MicroRNAs
  • 3108 Plant biology
  • 3004 Crop and pasture production
  • 0703 Crop and Pasture Production