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Voltage clamping with a single microelectrode.

Publication ,  Journal Article
Wilson, WA; Goldner, MM
Published in: Journal of neurobiology
July 1975

A technique is described which allows neurons to be voltage clamped with a single microelectrode, and the advantages of this circuit with respect to conventional bridge techniques are discussed. In this circuit, the single microelectrode is rapidly switched from a current passing to a recording mode. The circuitry consists of: (1) an electronic switch; (2) a high impedance, ultralow input capacity amplifier; (3) a sample-and-hold module; (4) conventional voltage clamping circuitry. The closed electronic switch allows current to flow through the electrode. The switch then opens, and the electrode is in a recording mode. The low input capacity of the preamplifier allows the artifact from the current pulse to rapidly abate, after which time the circuit samples the membrane potential. This cycle is repeated at rates up to 10 kHz. The voltage clamping amplifier senses the output of the sample-and-hold module and adjusts the current pulse amplitude to maintain the desired membrane potential. The system was evaluated in Aplysia neurons by inserting two microelectrodes into a cell. One electrode was used to clamp the cell and the other to independently monitor membrane potential at a remote location in the soma.

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Published In

Journal of neurobiology

DOI

EISSN

1097-4695

ISSN

0022-3034

Publication Date

July 1975

Volume

6

Issue

4

Start / End Page

411 / 422

Related Subject Headings

  • Neurophysiology
  • Neurology & Neurosurgery
  • Mollusca
  • Membrane Potentials
  • Electrophysiology
  • Animals
  • 3209 Neurosciences
  • 1702 Cognitive Sciences
  • 1116 Medical Physiology
  • 1109 Neurosciences
 

Citation

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Wilson, W. A., & Goldner, M. M. (1975). Voltage clamping with a single microelectrode. Journal of Neurobiology, 6(4), 411–422. https://doi.org/10.1002/neu.480060406
Wilson, W. A., and M. M. Goldner. “Voltage clamping with a single microelectrode.Journal of Neurobiology 6, no. 4 (July 1975): 411–22. https://doi.org/10.1002/neu.480060406.
Wilson WA, Goldner MM. Voltage clamping with a single microelectrode. Journal of neurobiology. 1975 Jul;6(4):411–22.
Wilson, W. A., and M. M. Goldner. “Voltage clamping with a single microelectrode.Journal of Neurobiology, vol. 6, no. 4, July 1975, pp. 411–22. Epmc, doi:10.1002/neu.480060406.
Wilson WA, Goldner MM. Voltage clamping with a single microelectrode. Journal of neurobiology. 1975 Jul;6(4):411–422.

Published In

Journal of neurobiology

DOI

EISSN

1097-4695

ISSN

0022-3034

Publication Date

July 1975

Volume

6

Issue

4

Start / End Page

411 / 422

Related Subject Headings

  • Neurophysiology
  • Neurology & Neurosurgery
  • Mollusca
  • Membrane Potentials
  • Electrophysiology
  • Animals
  • 3209 Neurosciences
  • 1702 Cognitive Sciences
  • 1116 Medical Physiology
  • 1109 Neurosciences