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A Mast Cell Degranulation Screening Assay for the Identification of Novel Mast Cell Activating Agents.

Publication ,  Journal Article
Staats, HF; Kirwan, SM; Choi, HW; Shelburne, CP; Abraham, SN; Leung, GYC; Chen, DY-K
Published in: Medchemcomm
January 1, 2013

The development and use of vaccines and their ability to prevent infection/disease is a shining example of the benefit of biomedical research. Modern vaccines often utilize subunit immunogens that exhibit minimal immunogenicity and require the use of adjuvants to maximize the induction of protective immune responses. We recently described a novel class of vaccine adjuvants, mast cell (MC) activators, that exhibit safe and effective vaccine adjuvant activity when administered by intranasal or intradermal routes. A compound library containing 580 functionalized benzopyrans, a structural motif found in a diverse array of natural and designed bioactive compounds, was screened using a MC degranulation assay to identify novel MC activating compounds for future evaluation as novel vaccine adjuvants. This approach identified 12 novel MC degranulating compounds. Therefore, MC degranulation can be used to reliably detect novel compounds for evaluation as adjuvants for use in mucosal vaccine strategies.

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Published In

Medchemcomm

DOI

ISSN

2040-2503

Publication Date

January 1, 2013

Volume

4

Issue

1

Location

England

Related Subject Headings

  • 3405 Organic chemistry
  • 3404 Medicinal and biomolecular chemistry
  • 1115 Pharmacology and Pharmaceutical Sciences
  • 0305 Organic Chemistry
  • 0304 Medicinal and Biomolecular Chemistry
 

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Staats, H. F., Kirwan, S. M., Choi, H. W., Shelburne, C. P., Abraham, S. N., Leung, G. Y. C., & Chen, D.-K. (2013). A Mast Cell Degranulation Screening Assay for the Identification of Novel Mast Cell Activating Agents. Medchemcomm, 4(1). https://doi.org/10.1039/C2MD20073B
Staats, Herman F., Shaun M. Kirwan, Hae Woong Choi, Christopher P. Shelburne, Soman N. Abraham, Gulice Y. C. Leung, and David Y-K Chen. “A Mast Cell Degranulation Screening Assay for the Identification of Novel Mast Cell Activating Agents.Medchemcomm 4, no. 1 (January 1, 2013). https://doi.org/10.1039/C2MD20073B.
Staats HF, Kirwan SM, Choi HW, Shelburne CP, Abraham SN, Leung GYC, et al. A Mast Cell Degranulation Screening Assay for the Identification of Novel Mast Cell Activating Agents. Medchemcomm. 2013 Jan 1;4(1).
Staats, Herman F., et al. “A Mast Cell Degranulation Screening Assay for the Identification of Novel Mast Cell Activating Agents.Medchemcomm, vol. 4, no. 1, Jan. 2013. Pubmed, doi:10.1039/C2MD20073B.
Staats HF, Kirwan SM, Choi HW, Shelburne CP, Abraham SN, Leung GYC, Chen DY-K. A Mast Cell Degranulation Screening Assay for the Identification of Novel Mast Cell Activating Agents. Medchemcomm. 2013 Jan 1;4(1).
Journal cover image

Published In

Medchemcomm

DOI

ISSN

2040-2503

Publication Date

January 1, 2013

Volume

4

Issue

1

Location

England

Related Subject Headings

  • 3405 Organic chemistry
  • 3404 Medicinal and biomolecular chemistry
  • 1115 Pharmacology and Pharmaceutical Sciences
  • 0305 Organic Chemistry
  • 0304 Medicinal and Biomolecular Chemistry