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Effect of SNMC on inhibiting hepatocyte apoptosis induced by Fas/FasL in mice with fulminant hepatic failure

Publication ,  Journal Article
Bi, MR; Yang, BS; Ma, YJ; Chen, LY; Wang, Y; Gao, F; Wang, FX
Published in: World Chinese Journal of Digestology
January 1, 2005

AIM: To study the effect of Stronger Neo-Minophagen C (SNMC) on inhibiting hepatocyte apoptosis in mice with fulminant hepatic failure (FHF) and its mechanism. METHODS: Seventy mice were divided randomly into three groups: A (normal control group, n = 5), B (model group, n = 5) and C (SNMC-protecting group, n = 60). Mice in groups A and B were killed 6 h after treated with SNMC. Mice in group C were killed 6 h, 1, 3, 5 and 7 d after treated with SNMC respectively, five mice for each time. The hepatocyte apoptosis in situ was detected by TUNEL. The ultrastructure of hepatocytes was examined by electron microscopy. The expression of Fas, FasL and caspase-3 was detected by immunohistochemistry. RESULTS: The hepatocyte apoptosis index was 32.3% 6 h after treated with SNMC in group C, which was decreased to 5% after 7 d. The apoptosis index was significantly lower 1, 3, 5 and 7 d after treated with SNMC in group C than that in group B (P <0.01). Small amount of Fas and caspase-3 were expressed in group A, while no FasL was expressed. The cells with Fas, FasL and Caspase-3 expression were increased significantly. However, Fas, FasL and Caspase-3 expression was decreased with the increasing of time in group C, and their levels were significantly lower 1, 3, 5 and 7 d after treated with SNMC than those in group B (P<0.01). CONCLUSION: SNMC can effectively protect hepatocytes from apoptosis in mice with FHF, and the mechanism may be related to its effect on the inhibition of Fas/FasL.

Duke Scholars

Published In

World Chinese Journal of Digestology

DOI

ISSN

1009-3079

Publication Date

January 1, 2005

Volume

13

Issue

1

Start / End Page

26 / 30

Related Subject Headings

  • Gastroenterology & Hepatology
 

Citation

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Bi, M. R., Yang, B. S., Ma, Y. J., Chen, L. Y., Wang, Y., Gao, F., & Wang, F. X. (2005). Effect of SNMC on inhibiting hepatocyte apoptosis induced by Fas/FasL in mice with fulminant hepatic failure. World Chinese Journal of Digestology, 13(1), 26–30. https://doi.org/10.11569/wcjd.v13.i1.26
Bi, M. R., B. S. Yang, Y. J. Ma, L. Y. Chen, Y. Wang, F. Gao, and F. X. Wang. “Effect of SNMC on inhibiting hepatocyte apoptosis induced by Fas/FasL in mice with fulminant hepatic failure.” World Chinese Journal of Digestology 13, no. 1 (January 1, 2005): 26–30. https://doi.org/10.11569/wcjd.v13.i1.26.
Bi MR, Yang BS, Ma YJ, Chen LY, Wang Y, Gao F, et al. Effect of SNMC on inhibiting hepatocyte apoptosis induced by Fas/FasL in mice with fulminant hepatic failure. World Chinese Journal of Digestology. 2005 Jan 1;13(1):26–30.
Bi, M. R., et al. “Effect of SNMC on inhibiting hepatocyte apoptosis induced by Fas/FasL in mice with fulminant hepatic failure.” World Chinese Journal of Digestology, vol. 13, no. 1, Jan. 2005, pp. 26–30. Scopus, doi:10.11569/wcjd.v13.i1.26.
Bi MR, Yang BS, Ma YJ, Chen LY, Wang Y, Gao F, Wang FX. Effect of SNMC on inhibiting hepatocyte apoptosis induced by Fas/FasL in mice with fulminant hepatic failure. World Chinese Journal of Digestology. 2005 Jan 1;13(1):26–30.

Published In

World Chinese Journal of Digestology

DOI

ISSN

1009-3079

Publication Date

January 1, 2005

Volume

13

Issue

1

Start / End Page

26 / 30

Related Subject Headings

  • Gastroenterology & Hepatology