Preparation and characterization of bioresorbable synthetic scaffolds composed from different beta-tricalcium phosphate powders and collagen
In the present study, two variants of COL/β-TCP scaffolds (P1 and P2) were prepared and examined. Microcrystalline b-TCP powders were prepared at high temperatures by solid-state reactions, and the nano-powders were produced by a precipitation technique. The collagen-TCP sponges were obtained by the reaction of two kinds of β-TCP powders (micro and nanometric size powders) with type I collagen under the physiological conditions. The morphology and chemical composition were analyzed semiquantitatively using X-ray diffraction analysis (XRD), scanning electron microscopy (SEM) and infrared spectroscopy (IR). The biocompatibility of the materials was evaluated by cytotoxic effects (cell death and cell proliferation). The biocompatibility of the obtained scaffolds was tested by biochemical and histological methods on human fibroblast cultures.
