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Comparative genome analysis of Trichophyton rubrum and related dermatophytes reveals candidate genes involved in infection.

Publication ,  Journal Article
Martinez, DA; Oliver, BG; Gräser, Y; Goldberg, JM; Li, W; Martinez-Rossi, NM; Monod, M; Shelest, E; Barton, RC; Birch, E; Brakhage, AA; Xu, J ...
Published in: mBio
December 1, 2012

The major cause of athlete's foot is Trichophyton rubrum, a dermatophyte or fungal pathogen of human skin. To facilitate molecular analyses of the dermatophytes, we sequenced T. rubrum and four related species, Trichophyton tonsurans, Trichophyton equinum, Microsporum canis, and Microsporum gypseum. These species differ in host range, mating, and disease progression. The dermatophyte genomes are highly colinear yet contain gene family expansions not found in other human-associated fungi. Dermatophyte genomes are enriched for gene families containing the LysM domain, which binds chitin and potentially related carbohydrates. These LysM domains differ in sequence from those in other species in regions of the peptide that could affect substrate binding. The dermatophytes also encode novel sets of fungus-specific kinases with unknown specificity, including nonfunctional pseudokinases, which may inhibit phosphorylation by competing for kinase sites within substrates, acting as allosteric effectors, or acting as scaffolds for signaling. The dermatophytes are also enriched for a large number of enzymes that synthesize secondary metabolites, including dermatophyte-specific genes that could synthesize novel compounds. Finally, dermatophytes are enriched in several classes of proteases that are necessary for fungal growth and nutrient acquisition on keratinized tissues. Despite differences in mating ability, genes involved in mating and meiosis are conserved across species, suggesting the possibility of cryptic mating in species where it has not been previously detected. These genome analyses identify gene families that are important to our understanding of how dermatophytes cause chronic infections, how they interact with epithelial cells, and how they respond to the host immune response.

Duke Scholars

Published In

mBio

EISSN

2150-7511

Publication Date

December 1, 2012

Volume

3

Issue

5

Related Subject Headings

  • 3207 Medical microbiology
  • 3107 Microbiology
  • 3101 Biochemistry and cell biology
  • 0605 Microbiology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Martinez, D. A., Oliver, B. G., Gräser, Y., Goldberg, J. M., Li, W., Martinez-Rossi, N. M., … White, T. C. (2012). Comparative genome analysis of Trichophyton rubrum and related dermatophytes reveals candidate genes involved in infection. MBio, 3(5).
Martinez, D. A., B. G. Oliver, Y. Gräser, J. M. Goldberg, W. Li, N. M. Martinez-Rossi, M. Monod, et al. “Comparative genome analysis of Trichophyton rubrum and related dermatophytes reveals candidate genes involved in infection.MBio 3, no. 5 (December 1, 2012).
Martinez DA, Oliver BG, Gräser Y, Goldberg JM, Li W, Martinez-Rossi NM, et al. Comparative genome analysis of Trichophyton rubrum and related dermatophytes reveals candidate genes involved in infection. mBio. 2012 Dec 1;3(5).
Martinez DA, Oliver BG, Gräser Y, Goldberg JM, Li W, Martinez-Rossi NM, Monod M, Shelest E, Barton RC, Birch E, Brakhage AA, Chen Z, Gurr SJ, Heiman D, Heitman J, Kosti I, Rossi A, Saif S, Samalova M, Saunders CW, Shea T, Summerbell RC, Xu J, Young S, Zeng Q, Birren BW, Cuomo CA, White TC. Comparative genome analysis of Trichophyton rubrum and related dermatophytes reveals candidate genes involved in infection. mBio. 2012 Dec 1;3(5).

Published In

mBio

EISSN

2150-7511

Publication Date

December 1, 2012

Volume

3

Issue

5

Related Subject Headings

  • 3207 Medical microbiology
  • 3107 Microbiology
  • 3101 Biochemistry and cell biology
  • 0605 Microbiology