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What functions of the sperm cell are measured by in vitro fertilization of zona-free hamster eggs?

Publication ,  Journal Article
Gould, JE; Overstreet, JW; Yanagimachi, H; Yanagimachi, R; Katz, DF; Hanson, FW
Published in: Fertility and sterility
September 1983

Human spermatozoa were capacitated in media containing either high concentrations (3.5%) of human serum albumin (HSA) or low concentrations (0.3%) of bovine serum albumin (BSA). The effects of both capacitation media were assessed immediately and after overnight preincubation (18 to 24 hours) by adding a mixture of nonliving human oocytes and living zona-free hamster eggs to the sperm suspension. Overnight preincubation of sperm in media containing 3.5% HSA enhanced sperm fusion with hamster vitelli, but the capacity for zona penetration was lost. These effects could be attributed to the concentration of HSA rather than a general effect of albumin concentration, because overnight preincubation in 3.5% BSA did not interfere with zona penetration. During overnight incubation in 3.5% HSA, the percentage of acrosome reactions increased, as did alterations in the equatorial segment of the acrosome-reacted sperm. The percentage of motile sperm remained high after overnight incubation in 3.5% HSA, but both the mean swimming speed and flagellar activity on the zona surface declined. The sperm also lost their ability for strong zona binding after overnight incubation in 3.5% HSA.

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Published In

Fertility and sterility

DOI

EISSN

1556-5653

ISSN

0015-0282

Publication Date

September 1983

Volume

40

Issue

3

Start / End Page

344 / 352

Related Subject Headings

  • Videotape Recording
  • Spermatozoa
  • Sperm-Ovum Interactions
  • Sperm Motility
  • Obstetrics & Reproductive Medicine
  • Male
  • Humans
  • Fertilization
  • Female
  • Cricetinae
 

Citation

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Gould, J. E., Overstreet, J. W., Yanagimachi, H., Yanagimachi, R., Katz, D. F., & Hanson, F. W. (1983). What functions of the sperm cell are measured by in vitro fertilization of zona-free hamster eggs? Fertility and Sterility, 40(3), 344–352. https://doi.org/10.1016/s0015-0282(16)47298-5
Gould, J. E., J. W. Overstreet, H. Yanagimachi, R. Yanagimachi, D. F. Katz, and F. W. Hanson. “What functions of the sperm cell are measured by in vitro fertilization of zona-free hamster eggs?Fertility and Sterility 40, no. 3 (September 1983): 344–52. https://doi.org/10.1016/s0015-0282(16)47298-5.
Gould JE, Overstreet JW, Yanagimachi H, Yanagimachi R, Katz DF, Hanson FW. What functions of the sperm cell are measured by in vitro fertilization of zona-free hamster eggs? Fertility and sterility. 1983 Sep;40(3):344–52.
Gould, J. E., et al. “What functions of the sperm cell are measured by in vitro fertilization of zona-free hamster eggs?Fertility and Sterility, vol. 40, no. 3, Sept. 1983, pp. 344–52. Epmc, doi:10.1016/s0015-0282(16)47298-5.
Gould JE, Overstreet JW, Yanagimachi H, Yanagimachi R, Katz DF, Hanson FW. What functions of the sperm cell are measured by in vitro fertilization of zona-free hamster eggs? Fertility and sterility. 1983 Sep;40(3):344–352.
Journal cover image

Published In

Fertility and sterility

DOI

EISSN

1556-5653

ISSN

0015-0282

Publication Date

September 1983

Volume

40

Issue

3

Start / End Page

344 / 352

Related Subject Headings

  • Videotape Recording
  • Spermatozoa
  • Sperm-Ovum Interactions
  • Sperm Motility
  • Obstetrics & Reproductive Medicine
  • Male
  • Humans
  • Fertilization
  • Female
  • Cricetinae