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Sensory neuron signaling to the brain: properties of transmitter release from olfactory nerve terminals.

Publication ,  Journal Article
Murphy, GJ; Glickfeld, LL; Balsen, Z; Isaacson, JS
Published in: J Neurosci
March 24, 2004

Olfactory receptor neurons (ORNs) convey sensory information directly to the CNS via conventional glutamatergic synaptic contacts in olfactory bulb glomeruli. To better understand the process by which information contained in the odorant-evoked firing of ORNs is transmitted to the brain, we examined the properties of glutamate release from olfactory nerve (ON) terminals in slices of the rat olfactory bulb. We show that marked paired pulse depression is the same in simultaneously recorded periglomerular and tufted neurons, and that this form of short-term plasticity is attributable to a reduction of glutamate release from ON terminals. We used the progressive blockade of NMDA receptor (NMDAR) EPSCs by MK-801 [(5R,10S)-(+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5-10-imine hydrogen maleate] and stationary fluctuation analysis of AMPA receptor (AMPAR) EPSCs to determine the probability of release (P(r)) of ON terminals; both approaches indicated that P(r) is unusually high (>/=0.8). The low-affinity glutamate receptor antagonists gamma-d-glutamylglycine and l-amino-5-phosphonovaleric acid blocked ON-evoked AMPAR- and NMDAR-mediated EPSCs, respectively, to the same extent under conditions of low and high P(r), suggesting that multivesicular release is not a feature of ON terminals. Although release from most synapses exhibits a highly nonlinear dependence on extracellular Ca(2+), we find that the relationship between glutamate release and extracellular Ca(2+) at ON terminals is nearly linear. Our results suggest that ON terminals have specialized features that may contribute to the reliable transmission of sensory information from nose to brain.

Duke Scholars

Published In

J Neurosci

DOI

EISSN

1529-2401

Publication Date

March 24, 2004

Volume

24

Issue

12

Start / End Page

3023 / 3030

Location

United States

Related Subject Headings

  • Synaptic Transmission
  • Receptors, N-Methyl-D-Aspartate
  • Receptors, Glutamate
  • Receptors, AMPA
  • Rats, Sprague-Dawley
  • Rats
  • Presynaptic Terminals
  • Olfactory Nerve
  • Olfactory Bulb
  • Neurotransmitter Agents
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Murphy, G. J., Glickfeld, L. L., Balsen, Z., & Isaacson, J. S. (2004). Sensory neuron signaling to the brain: properties of transmitter release from olfactory nerve terminals. J Neurosci, 24(12), 3023–3030. https://doi.org/10.1523/JNEUROSCI.5745-03.2004
Murphy, Gabe J., Lindsey L. Glickfeld, Zev Balsen, and Jeffry S. Isaacson. “Sensory neuron signaling to the brain: properties of transmitter release from olfactory nerve terminals.J Neurosci 24, no. 12 (March 24, 2004): 3023–30. https://doi.org/10.1523/JNEUROSCI.5745-03.2004.
Murphy GJ, Glickfeld LL, Balsen Z, Isaacson JS. Sensory neuron signaling to the brain: properties of transmitter release from olfactory nerve terminals. J Neurosci. 2004 Mar 24;24(12):3023–30.
Murphy, Gabe J., et al. “Sensory neuron signaling to the brain: properties of transmitter release from olfactory nerve terminals.J Neurosci, vol. 24, no. 12, Mar. 2004, pp. 3023–30. Pubmed, doi:10.1523/JNEUROSCI.5745-03.2004.
Murphy GJ, Glickfeld LL, Balsen Z, Isaacson JS. Sensory neuron signaling to the brain: properties of transmitter release from olfactory nerve terminals. J Neurosci. 2004 Mar 24;24(12):3023–3030.

Published In

J Neurosci

DOI

EISSN

1529-2401

Publication Date

March 24, 2004

Volume

24

Issue

12

Start / End Page

3023 / 3030

Location

United States

Related Subject Headings

  • Synaptic Transmission
  • Receptors, N-Methyl-D-Aspartate
  • Receptors, Glutamate
  • Receptors, AMPA
  • Rats, Sprague-Dawley
  • Rats
  • Presynaptic Terminals
  • Olfactory Nerve
  • Olfactory Bulb
  • Neurotransmitter Agents