MicroRNA target site identification by integrating sequence and binding information.
Publication
, Journal Article
Majoros, WH; Lekprasert, P; Mukherjee, N; Skalsky, RL; Corcoran, DL; Cullen, BR; Ohler, U
Published in: Nat Methods
July 2013
High-throughput sequencing has opened numerous possibilities for the identification of regulatory RNA-binding events. Cross-linking and immunoprecipitation of Argonaute proteins can pinpoint a microRNA (miRNA) target site within tens of bases but leaves the identity of the miRNA unresolved. A flexible computational framework, microMUMMIE, integrates sequence with cross-linking features and reliably identifies the miRNA family involved in each binding event. It considerably outperforms sequence-only approaches and quantifies the prevalence of noncanonical binding modes.
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Published In
Nat Methods
DOI
EISSN
1548-7105
Publication Date
July 2013
Volume
10
Issue
7
Start / End Page
630 / 633
Location
United States
Related Subject Headings
- Systems Integration
- Sequence Analysis, RNA
- RNA-Binding Proteins
- RNA
- Protein Interaction Mapping
- Developmental Biology
- Algorithms
- 31 Biological sciences
- 11 Medical and Health Sciences
- 10 Technology
Citation
APA
Chicago
ICMJE
MLA
NLM
Majoros, W. H., Lekprasert, P., Mukherjee, N., Skalsky, R. L., Corcoran, D. L., Cullen, B. R., & Ohler, U. (2013). MicroRNA target site identification by integrating sequence and binding information. Nat Methods, 10(7), 630–633. https://doi.org/10.1038/nmeth.2489
Majoros, William H., Parawee Lekprasert, Neelanjan Mukherjee, Rebecca L. Skalsky, David L. Corcoran, Bryan R. Cullen, and Uwe Ohler. “MicroRNA target site identification by integrating sequence and binding information.” Nat Methods 10, no. 7 (July 2013): 630–33. https://doi.org/10.1038/nmeth.2489.
Majoros WH, Lekprasert P, Mukherjee N, Skalsky RL, Corcoran DL, Cullen BR, et al. MicroRNA target site identification by integrating sequence and binding information. Nat Methods. 2013 Jul;10(7):630–3.
Majoros, William H., et al. “MicroRNA target site identification by integrating sequence and binding information.” Nat Methods, vol. 10, no. 7, July 2013, pp. 630–33. Pubmed, doi:10.1038/nmeth.2489.
Majoros WH, Lekprasert P, Mukherjee N, Skalsky RL, Corcoran DL, Cullen BR, Ohler U. MicroRNA target site identification by integrating sequence and binding information. Nat Methods. 2013 Jul;10(7):630–633.
Published In
Nat Methods
DOI
EISSN
1548-7105
Publication Date
July 2013
Volume
10
Issue
7
Start / End Page
630 / 633
Location
United States
Related Subject Headings
- Systems Integration
- Sequence Analysis, RNA
- RNA-Binding Proteins
- RNA
- Protein Interaction Mapping
- Developmental Biology
- Algorithms
- 31 Biological sciences
- 11 Medical and Health Sciences
- 10 Technology