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L-type calcium channels play a critical role in maintaining lens transparency by regulating phosphorylation of aquaporin-0 and myosin light chain and expression of connexins.

Publication ,  Journal Article
Maddala, R; Nagendran, T; de Ridder, GG; Schey, KL; Rao, PV
Published in: PLoS One
2013

Homeostasis of intracellular calcium is crucial for lens cytoarchitecture and transparency, however, the identity of specific channel proteins regulating calcium influx within the lens is not completely understood. Here we examined the expression and distribution profiles of L-type calcium channels (LTCCs) and explored their role in morphological integrity and transparency of the mouse lens, using cDNA microarray, RT-PCR, immunoblot, pharmacological inhibitors and immunofluorescence analyses. The results revealed that Ca (V) 1.2 and 1.3 channels are expressed and distributed in both the epithelium and cortical fiber cells in mouse lens. Inhibition of LTCCs with felodipine or nifedipine induces progressive cortical cataract formation with time, in association with decreased lens weight in ex-vivo mouse lenses. Histological analyses of felodipine treated lenses revealed extensive disorganization and swelling of cortical fiber cells resembling the phenotype reported for altered aquaporin-0 activity without detectable cytotoxic effects. Analysis of both soluble and membrane rich fractions from felodipine treated lenses by SDS-PAGE in conjunction with mass spectrometry and immunoblot analyses revealed decreases in β-B1-crystallin, Hsp-90, spectrin and filensin. Significantly, loss of transparency in the felodipine treated lenses was preceded by an increase in aquaporin-0 serine-235 phosphorylation and levels of connexin-50, together with decreases in myosin light chain phosphorylation and the levels of 14-3-3ε, a phosphoprotein-binding regulatory protein. Felodipine treatment led to a significant increase in gene expression of connexin-50 and 46 in the mouse lens. Additionally, felodipine inhibition of LTCCs in primary cultures of mouse lens epithelial cells resulted in decreased intracellular calcium, and decreased actin stress fibers and myosin light chain phosphorylation, without detectable cytotoxic response. Taken together, these observations reveal a crucial role for LTCCs in regulation of expression, activity and stability of aquaporin-0, connexins, cytoskeletal proteins, and the mechanical properties of lens, all of which have a vital role in maintaining lens function and cytoarchitecture.

Duke Scholars

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2013

Volume

8

Issue

5

Start / End Page

e64676

Location

United States

Related Subject Headings

  • beta-Crystallin B Chain
  • Reverse Transcriptase Polymerase Chain Reaction
  • Phosphorylation
  • Nifedipine
  • Myosin Light Chains
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Lens, Crystalline
  • Immunoblotting
 

Citation

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ICMJE
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Maddala, R., Nagendran, T., de Ridder, G. G., Schey, K. L., & Rao, P. V. (2013). L-type calcium channels play a critical role in maintaining lens transparency by regulating phosphorylation of aquaporin-0 and myosin light chain and expression of connexins. PLoS One, 8(5), e64676. https://doi.org/10.1371/journal.pone.0064676
Maddala, Rupalatha, Tharkika Nagendran, Gustaaf G. de Ridder, Kevin L. Schey, and Ponugoti Vasantha Rao. “L-type calcium channels play a critical role in maintaining lens transparency by regulating phosphorylation of aquaporin-0 and myosin light chain and expression of connexins.PLoS One 8, no. 5 (2013): e64676. https://doi.org/10.1371/journal.pone.0064676.
Maddala, Rupalatha, et al. “L-type calcium channels play a critical role in maintaining lens transparency by regulating phosphorylation of aquaporin-0 and myosin light chain and expression of connexins.PLoS One, vol. 8, no. 5, 2013, p. e64676. Pubmed, doi:10.1371/journal.pone.0064676.

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2013

Volume

8

Issue

5

Start / End Page

e64676

Location

United States

Related Subject Headings

  • beta-Crystallin B Chain
  • Reverse Transcriptase Polymerase Chain Reaction
  • Phosphorylation
  • Nifedipine
  • Myosin Light Chains
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Lens, Crystalline
  • Immunoblotting