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Oxidatively modified proteins as plasma biomarkers in breast cancer.

Publication ,  Journal Article
Jin, H; Daly, DS; Marks, JR; Zangar, RC
Published in: Cancer Biomark
2013

BACKGROUND: Post-translational protein modifications (PTMs) are increased in breast tumors. OBJECTIVE: We explored whether PTMs on proteins secreted by the breast could be detected in plasma and potentially used for the early detection of breast cancer. METHODS: We used a custom ELISA microarray platform to measure 4-hydroxynonenal (HNE), glutathione (GSH), nitrotyrosine and halotyrosine adducts in 27 secreted proteins, for a total of 108 candidate biomarkers. Two independent sets of human plasma samples were measured, for a total of 160 samples. The results were analyzed for consistent cancer-associated changes across the two sample sets. Plasma samples for both cases and benign controls were collected at the time of tissue diagnosis after referral from a positive screen (such as mammography). The results from both studies were evaluated using ANOVA and t-tests or receiver operator curves (ROC). RESULTS: Levels of GSH-modified ceruloplasmin and HNE-modified PDGF were significantly altered in plasma samples from cancer patients relative to benign controls. Healthy controls, which were only included in the first set of samples, were similar to the benign controls for both of these markers. A combination of three glutathionylated proteins produced the best area under the ROC curve, with a value of 76%. CONCLUSIONS: Specific PTMs in individual proteins may be useful for distinguishing between women with breast cancer and those with benign breast disease. These oxidative changes in plasma proteins may reflect redox changes in breast cancer. Additional studies on oxidative modifications in individual proteins are warranted.

Duke Scholars

Published In

Cancer Biomark

DOI

EISSN

1875-8592

Publication Date

2013

Volume

13

Issue

3

Start / End Page

193 / 200

Location

Netherlands

Related Subject Headings

  • Reactive Oxygen Species
  • Protein Processing, Post-Translational
  • Oxidation-Reduction
  • Oncology & Carcinogenesis
  • Middle Aged
  • Microarray Analysis
  • Humans
  • Female
  • Enzyme-Linked Immunosorbent Assay
  • Breast Neoplasms
 

Citation

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Jin, H., Daly, D. S., Marks, J. R., & Zangar, R. C. (2013). Oxidatively modified proteins as plasma biomarkers in breast cancer. Cancer Biomark, 13(3), 193–200. https://doi.org/10.3233/CBM-130349
Jin, Hongjun, Don S. Daly, Jeffrey R. Marks, and Richard C. Zangar. “Oxidatively modified proteins as plasma biomarkers in breast cancer.Cancer Biomark 13, no. 3 (2013): 193–200. https://doi.org/10.3233/CBM-130349.
Jin H, Daly DS, Marks JR, Zangar RC. Oxidatively modified proteins as plasma biomarkers in breast cancer. Cancer Biomark. 2013;13(3):193–200.
Jin, Hongjun, et al. “Oxidatively modified proteins as plasma biomarkers in breast cancer.Cancer Biomark, vol. 13, no. 3, 2013, pp. 193–200. Pubmed, doi:10.3233/CBM-130349.
Jin H, Daly DS, Marks JR, Zangar RC. Oxidatively modified proteins as plasma biomarkers in breast cancer. Cancer Biomark. 2013;13(3):193–200.

Published In

Cancer Biomark

DOI

EISSN

1875-8592

Publication Date

2013

Volume

13

Issue

3

Start / End Page

193 / 200

Location

Netherlands

Related Subject Headings

  • Reactive Oxygen Species
  • Protein Processing, Post-Translational
  • Oxidation-Reduction
  • Oncology & Carcinogenesis
  • Middle Aged
  • Microarray Analysis
  • Humans
  • Female
  • Enzyme-Linked Immunosorbent Assay
  • Breast Neoplasms