Effects of cellular activation on anti-HIV-1 restriction factor expression profile in primary cells.
Expression of cell-intrinsic antiviral factors suppresses HIV-1 replication. We hypothesized that cellular activation modulates host restriction and susceptibility to HIV-1 infection. We measured the gene expression of 34 antiviral factors in healthy peripheral blood mononuclear cells (PBMC). Cellular activation induced expression of interferon-stimulated gene 15 (ISG15), tripartite motif 5α (TRIM5α), bone marrow stromal cell antigen 2 (BST-2)/tetherin, and certain apolipoprotein B mRNA editing enzyme 3 (APOBEC3) family members. Expression of RTF1, RNA polymerase II-associated factor 1 (PAF1), TRIM11, TRIM26, and BST-2/tetherin correlated with decreased HIV-1 infectivity. This report demonstrates synchronous effects of activation-induced antiviral genes on HIV-1 infectivity, providing candidates for pharmacological manipulation.
Duke Scholars
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Related Subject Headings
- Virology
- Leukocytes, Mononuclear
- Immunity, Innate
- Humans
- HIV-1
- Gene Expression Profiling
- Cells, Cultured
- 32 Biomedical and clinical sciences
- 31 Biological sciences
- 30 Agricultural, veterinary and food sciences
Citation
Published In
DOI
EISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Virology
- Leukocytes, Mononuclear
- Immunity, Innate
- Humans
- HIV-1
- Gene Expression Profiling
- Cells, Cultured
- 32 Biomedical and clinical sciences
- 31 Biological sciences
- 30 Agricultural, veterinary and food sciences