Skip to main content

Relationship between NaCl- and H2O2-induced cytosolic Ca2+ increases in response to stress in Arabidopsis.

Publication ,  Journal Article
Jiang, Z; Zhu, S; Ye, R; Xue, Y; Chen, A; An, L; Pei, Z-M
Published in: PloS one
January 2013

Salinity is among the environmental factors that affect plant growth and development and constrain agricultural productivity. Salinity stress triggers increases in cytosolic free Ca(2+) concentration ([Ca(2+)]i) via Ca(2+) influx across the plasma membrane. Salinity stress, as well as other stresses, induces the production of reactive oxygen species (ROS). It is well established that ROS also triggers increases in [Ca(2+)]i. However, the relationship and interaction between salinity stress-induced [Ca(2+)]i increases and ROS-induced [Ca(2+)]i increases remain poorly understood. Using an aequorin-based Ca(2+) imaging assay we have analyzed [Ca(2+)]i changes in response to NaCl and H2O2 treatments in Arabidopsis thaliana. We found that NaCl and H2O2 together induced larger increases in [Ca(2+)]i in Arabidopsis seedlings than either NaCl or H2O2 alone, suggesting an additive effect on [Ca(2+)]i increases. Following a pre-treatment with either NaCl or H2O2, the subsequent elevation of [Ca(2+)]i in response to a second treatment with either NaCl or H2O2 was significantly reduced. Furthermore, the NaCl pre-treatment suppressed the elevation of [Ca(2+)]i seen with a second NaCl treatment more than that seen with a second treatment of H2O2. A similar response was seen when the initial treatment was with H2O2; subsequent addition of H2O2 led to less of an increase in [Ca(2+)]i than did addition of NaCl. These results imply that NaCl-gated Ca(2+) channels and H2O2-gated Ca(2+) channels may differ, and also suggest that NaCl- and H2O2-evoked [Ca(2+)]i may reduce the potency of both NaCl and H2O2 in triggering [Ca(2+)]i increases, highlighting a feedback mechanism. Alternatively, NaCl and H2O2 may activate the same Ca(2+) permeable channel, which is expressed in different types of cells and/or activated via different signaling pathways.

Duke Scholars

Published In

PloS one

DOI

EISSN

1932-6203

ISSN

1932-6203

Publication Date

January 2013

Volume

8

Issue

10

Start / End Page

e76130

Related Subject Headings

  • Sodium Chloride
  • Signal Transduction
  • Reactive Oxygen Species
  • Hydrogen Peroxide
  • General Science & Technology
  • Cytosol
  • Calcium
  • Arabidopsis
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Jiang, Z., Zhu, S., Ye, R., Xue, Y., Chen, A., An, L., & Pei, Z.-M. (2013). Relationship between NaCl- and H2O2-induced cytosolic Ca2+ increases in response to stress in Arabidopsis. PloS One, 8(10), e76130. https://doi.org/10.1371/journal.pone.0076130
Jiang, Zhonghao, Shan Zhu, Rui Ye, Yan Xue, Amelia Chen, Lizhe An, and Zhen-Ming Pei. “Relationship between NaCl- and H2O2-induced cytosolic Ca2+ increases in response to stress in Arabidopsis.PloS One 8, no. 10 (January 2013): e76130. https://doi.org/10.1371/journal.pone.0076130.
Jiang Z, Zhu S, Ye R, Xue Y, Chen A, An L, et al. Relationship between NaCl- and H2O2-induced cytosolic Ca2+ increases in response to stress in Arabidopsis. PloS one. 2013 Jan;8(10):e76130.
Jiang, Zhonghao, et al. “Relationship between NaCl- and H2O2-induced cytosolic Ca2+ increases in response to stress in Arabidopsis.PloS One, vol. 8, no. 10, Jan. 2013, p. e76130. Epmc, doi:10.1371/journal.pone.0076130.
Jiang Z, Zhu S, Ye R, Xue Y, Chen A, An L, Pei Z-M. Relationship between NaCl- and H2O2-induced cytosolic Ca2+ increases in response to stress in Arabidopsis. PloS one. 2013 Jan;8(10):e76130.

Published In

PloS one

DOI

EISSN

1932-6203

ISSN

1932-6203

Publication Date

January 2013

Volume

8

Issue

10

Start / End Page

e76130

Related Subject Headings

  • Sodium Chloride
  • Signal Transduction
  • Reactive Oxygen Species
  • Hydrogen Peroxide
  • General Science & Technology
  • Cytosol
  • Calcium
  • Arabidopsis