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β2-Adrenergic receptors in hamster smooth muscle cells are transcriptionally regulated by glucocorticoids

Publication ,  Journal Article
Collins, S; Caron, MG; Lefkowitz, RJ
Published in: Journal of Biological Chemistry
January 1, 1988

Steroid hormones modulate adrenergic receptor responsiveness and receptor number. To investigate the regulation of the β2-adrenergic receptor gene by glucocorticoids we examined the effects of the synthetic glucocorticoid agonist triamcinolone acetonide on the expression of β2-adrenergic receptors in DDT1MF-2 hamster smooth muscle cells. Glucocorticoid treatment (1 x 10-7 M) produced a 2.2 ± 0.4-fold (n = 8) increase in β2-adrenergic receptor number (maximum between 6 and 12 h) as determined by radioligand binding and a similar increase in catecholamine-stimulated adenylate cyclase activity. Steady-state levels of β2-adrenergic receptor mRNA, analyzed by Northern blot hybridization, were increased 2.4 ± 0.4-fold (n = 6) within 1 h, while actin mRNA levels were unchanged throughout the experiment. These steroid-induced increases in β2-adrenergic receptor mRNA returned to control levels by 24 h and were followed by a much slower decline in β2-adrenergic receptor in plasma membranes. The rate of β2-adrenergic receptor gene transcription, assessed by nuclear run-off transcription assays, increased 3.1 ± 0.1-fold (n = 2) in cells treated for 30 min with 1 x 10-7 M triamcinolone acetonide. These studies indicate that glucocorticoids regulate the β2-adrenergic receptor-adenylate cyclase system by controlling the rate of transcription of the β2-adrenergic receptor gene and hence the responsiveness of the enzyme to catecholamine stimulation.

Duke Scholars

Published In

Journal of Biological Chemistry

ISSN

0021-9258

Publication Date

January 1, 1988

Volume

263

Issue

19

Start / End Page

9067 / 9070

Related Subject Headings

  • Biochemistry & Molecular Biology
  • 34 Chemical sciences
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences
  • 11 Medical and Health Sciences
  • 06 Biological Sciences
  • 03 Chemical Sciences
 

Citation

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Collins, S., Caron, M. G., & Lefkowitz, R. J. (1988). β2-Adrenergic receptors in hamster smooth muscle cells are transcriptionally regulated by glucocorticoids. Journal of Biological Chemistry, 263(19), 9067–9070.
Collins, S., M. G. Caron, and R. J. Lefkowitz. “β2-Adrenergic receptors in hamster smooth muscle cells are transcriptionally regulated by glucocorticoids.” Journal of Biological Chemistry 263, no. 19 (January 1, 1988): 9067–70.
Collins S, Caron MG, Lefkowitz RJ. β2-Adrenergic receptors in hamster smooth muscle cells are transcriptionally regulated by glucocorticoids. Journal of Biological Chemistry. 1988 Jan 1;263(19):9067–70.
Collins, S., et al. “β2-Adrenergic receptors in hamster smooth muscle cells are transcriptionally regulated by glucocorticoids.” Journal of Biological Chemistry, vol. 263, no. 19, Jan. 1988, pp. 9067–70.
Collins S, Caron MG, Lefkowitz RJ. β2-Adrenergic receptors in hamster smooth muscle cells are transcriptionally regulated by glucocorticoids. Journal of Biological Chemistry. 1988 Jan 1;263(19):9067–9070.

Published In

Journal of Biological Chemistry

ISSN

0021-9258

Publication Date

January 1, 1988

Volume

263

Issue

19

Start / End Page

9067 / 9070

Related Subject Headings

  • Biochemistry & Molecular Biology
  • 34 Chemical sciences
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences
  • 11 Medical and Health Sciences
  • 06 Biological Sciences
  • 03 Chemical Sciences