High-performance liquid chromatographic analysis of diltiazem and its metabolite in plasma.
Publication
, Journal Article
Verghese, C; Smith, MS; Aanonsen, L; Pritchett, EL; Shand, DG
Published in: J Chromatogr
January 14, 1983
A rapid, selective and reproducible high-performance liquid chromatographic method for the analysis in plasma of the calcium channel blocking agent, diltiazem, and one of its metabolites, deacetyldiltiazem is described. The method involves extraction with the methyl tert.-butyl ether of the drugs and the internal standard (verapamil), back-extraction into sulphuric acid and reversed-phase chromatography with UV detection. Over a concentration range of 10-1000 ng/ml the average coefficient of variation for diltiazem was 5.4% and for deacetyldiltiazem was 8.3%.
Duke Scholars
Published In
J Chromatogr
DOI
Publication Date
January 14, 1983
Volume
272
Issue
1
Start / End Page
149 / 155
Location
Netherlands
Related Subject Headings
- Humans
- Half-Life
- Diltiazem
- Chromatography, High Pressure Liquid
- Calcium Channel Blockers
- Benzazepines
Citation
APA
Chicago
ICMJE
MLA
NLM
Verghese, C., Smith, M. S., Aanonsen, L., Pritchett, E. L., & Shand, D. G. (1983). High-performance liquid chromatographic analysis of diltiazem and its metabolite in plasma. J Chromatogr, 272(1), 149–155. https://doi.org/10.1016/s0378-4347(00)86111-3
Verghese, C., M. S. Smith, L. Aanonsen, E. L. Pritchett, and D. G. Shand. “High-performance liquid chromatographic analysis of diltiazem and its metabolite in plasma.” J Chromatogr 272, no. 1 (January 14, 1983): 149–55. https://doi.org/10.1016/s0378-4347(00)86111-3.
Verghese C, Smith MS, Aanonsen L, Pritchett EL, Shand DG. High-performance liquid chromatographic analysis of diltiazem and its metabolite in plasma. J Chromatogr. 1983 Jan 14;272(1):149–55.
Verghese, C., et al. “High-performance liquid chromatographic analysis of diltiazem and its metabolite in plasma.” J Chromatogr, vol. 272, no. 1, Jan. 1983, pp. 149–55. Pubmed, doi:10.1016/s0378-4347(00)86111-3.
Verghese C, Smith MS, Aanonsen L, Pritchett EL, Shand DG. High-performance liquid chromatographic analysis of diltiazem and its metabolite in plasma. J Chromatogr. 1983 Jan 14;272(1):149–155.
Published In
J Chromatogr
DOI
Publication Date
January 14, 1983
Volume
272
Issue
1
Start / End Page
149 / 155
Location
Netherlands
Related Subject Headings
- Humans
- Half-Life
- Diltiazem
- Chromatography, High Pressure Liquid
- Calcium Channel Blockers
- Benzazepines