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Stage-specific and differential notch dependency at the alphabeta and gammadelta T lineage bifurcation.

Publication ,  Journal Article
Ciofani, M; Knowles, GC; Wiest, DL; von Boehmer, H; Zúñiga-Pflücker, JC
Published in: Immunity
July 2006

Signals transduced by Notch receptors are indispensable for T cell specification and differentiation of alphabeta T lineage cells. However, the role of Notch signals during alphabeta versus gammadelta T lineage decision remains controversial. Here, we addressed this question by employing a clonal analysis of CD4(-)CD8(-) (DN) progenitor potential to position the divergence of alphabeta and gammadelta T cell lineages to the late DN2 to DN3 developmental stages. Accordingly, alphabeta and gammadelta precursor frequencies within these T cell progenitor subsets were determined, both in the presence and absence of Notch signaling through Delta-like 1. Notch signals were found to be critical for the DN to CD4(+)CD8(+) (DP) transition, irrespective of the identity (pTalphabeta or gammadelta) of the inducing T cell receptor complex, whereas gammadelta T cells developed from gammadeltaTCR-expressing T cell progenitors in the absence of further Notch ligand interaction. Collectively, our findings demonstrate a differential, stage-specific requirement for Notch receptor-ligand interactions in the differentiation of alphabeta and gammadelta T cells from T cell progenitors.

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Published In

Immunity

DOI

ISSN

1074-7613

Publication Date

July 2006

Volume

25

Issue

1

Start / End Page

105 / 116

Location

United States

Related Subject Headings

  • Thymus Gland
  • T-Lymphocytes
  • Signal Transduction
  • Receptors, Notch
  • Receptors, Antigen, T-Cell
  • Mice
  • Ligands
  • Immunology
  • Hepatocytes
  • Hematopoietic Stem Cells
 

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Ciofani, M., Knowles, G. C., Wiest, D. L., von Boehmer, H., & Zúñiga-Pflücker, J. C. (2006). Stage-specific and differential notch dependency at the alphabeta and gammadelta T lineage bifurcation. Immunity, 25(1), 105–116. https://doi.org/10.1016/j.immuni.2006.05.010
Ciofani, Maria, Gisèle C. Knowles, David L. Wiest, Harald von Boehmer, and Juan Carlos Zúñiga-Pflücker. “Stage-specific and differential notch dependency at the alphabeta and gammadelta T lineage bifurcation.Immunity 25, no. 1 (July 2006): 105–16. https://doi.org/10.1016/j.immuni.2006.05.010.
Ciofani M, Knowles GC, Wiest DL, von Boehmer H, Zúñiga-Pflücker JC. Stage-specific and differential notch dependency at the alphabeta and gammadelta T lineage bifurcation. Immunity. 2006 Jul;25(1):105–16.
Ciofani, Maria, et al. “Stage-specific and differential notch dependency at the alphabeta and gammadelta T lineage bifurcation.Immunity, vol. 25, no. 1, July 2006, pp. 105–16. Pubmed, doi:10.1016/j.immuni.2006.05.010.
Ciofani M, Knowles GC, Wiest DL, von Boehmer H, Zúñiga-Pflücker JC. Stage-specific and differential notch dependency at the alphabeta and gammadelta T lineage bifurcation. Immunity. 2006 Jul;25(1):105–116.
Journal cover image

Published In

Immunity

DOI

ISSN

1074-7613

Publication Date

July 2006

Volume

25

Issue

1

Start / End Page

105 / 116

Location

United States

Related Subject Headings

  • Thymus Gland
  • T-Lymphocytes
  • Signal Transduction
  • Receptors, Notch
  • Receptors, Antigen, T-Cell
  • Mice
  • Ligands
  • Immunology
  • Hepatocytes
  • Hematopoietic Stem Cells