Grigory Mechetin

Journal Article International journal of molecular sciences · May 2023 The protein encoded by the vaccinia virus D4R gene has base excision repair uracil-DNA N-glycosylase (vvUNG) activity and also acts as a processivity factor in the viral replication complex. The use of a protein unlike PolN/PCNA sliding clamp ... Full text Cite

DNA Damage Response and Repair in Boron Neutron Capture Therapy.

Journal Article Genes · January 2023 Boron neutron capture therapy (BNCT) is an approach to the radiotherapy of solid tumors that was first outlined in the 1930s but has attracted considerable attention recently with the advent of a new generation of neutron sources. In BNCT, tumor cells accu ... Full text Cite

Distinct Mechanisms of Target Search by Endonuclease VIII-like DNA Glycosylases.

Journal Article Cells · October 2022 Proteins that recognize specific DNA sequences or structural elements often find their cognate DNA lesions in a processive mode, in which an enzyme binds DNA non-specifically and then slides along the DNA contour by one-dimensional diffusion. Opposite to t ... Full text Cite

Noncatalytic Domains in DNA Glycosylases.

Journal Article International journal of molecular sciences · June 2022 Many proteins consist of two or more structural domains: separate parts that have a defined structure and function. For example, in enzymes, the catalytic activity is often localized in a core fragment, while other domains or disordered parts of the same p ... Full text Cite

A New Class of Uracil-DNA Glycosylase Inhibitors Active against Human and Vaccinia Virus Enzyme.

Journal Article Molecules (Basel, Switzerland) · November 2021 Uracil-DNA glycosylases are enzymes that excise uracil bases appearing in DNA as a result of cytosine deamination or accidental dUMP incorporation from the dUTP pool. The activity of Family 1 uracil-DNA glycosylase (UNG) activity limits the efficiency of a ... Full text Cite

Characterization of Recombinant Endonuclease IV from Mycobacterium tuberculosis

Journal Article Molecular Biology · March 1, 2021 Abstract: Mycobacterium tuberculosis cells contain two apurinic/apyrimidinic (AP) endonucleases, endonuclease IV (MtbEnd) and exonuclease III (MtbXthA), the former playing a dominant role in protecting mycobacterial DNA from oxidative stress. Mycobacterial ... Full text Cite

Inhibitors of DNA Glycosylases as Prospective Drugs.

Journal Article International journal of molecular sciences · April 2020 DNA glycosylases are enzymes that initiate the base excision repair pathway, a major biochemical process that protects the genomes of all living organisms from intrinsically and environmentally inflicted damage. Recently, base excision repair inhibition pr ... Full text Cite

Correlated target search by uracil-DNA glycosylase in the presence of bulky adducts and DNA-binding ligands

Journal Article Russian Journal of Bioorganic Chemistry · January 1, 2017 Many proteins specific for rare targets in DNA, such as transcription factors, restriction endonucleases, and DNA repair enzymes, search for their targets by one-dimensional diffusion along DNA. One of these proteins is uracil-DNA glycosylase (Ung), which ... Full text Cite

Active destabilization of base pairs by a DNA glycosylase wedge initiates damage recognition.

Journal Article Nucleic acids research · January 2015 Formamidopyrimidine-DNA glycosylase (Fpg) excises 8-oxoguanine (oxoG) from DNA but ignores normal guanine. We combined molecular dynamics simulation and stopped-flow kinetics with fluorescence detection to track the events in the recognition of oxoG by Fpg ... Full text Cite

Mechanisms of diffusional search for specific targets by DNA-dependent proteins.

Journal Article Biochemistry. Biokhimiia · June 2014 To perform their functions, many DNA-dependent proteins have to quickly locate specific targets against the vast excess of nonspecific DNA. Although this problem was first formulated over 40 years ago, the mechanism of such search remains one of the unsolv ... Full text Cite

Mechanism of translocation of uracil-DNA glycosylase from Escherichia coli between distributed lesions.

Journal Article Biochemical and biophysical research communications · October 2011 Uracil-DNA glycosylase (Ung) is a DNA repair enzyme that excises uracil bases from DNA, where they appear through deamination of cytosine or incorporation from a cellular dUTP pool. DNA repair enzymes often use one-dimensional diffusion along DNA to accele ... Full text Cite

The mechanism of substrate search by base excision repair enzymes.

Journal Article Doklady. Biochemistry and biophysics · March 2011 Full text Cite

Uracil-DNA glycosylase: Structural, thermodynamic and kinetic aspects of lesion search and recognition.

Journal Article Mutation research · March 2010 Uracil appears in DNA as a result of cytosine deamination and by incorporation from the dUTP pool. As potentially mutagenic and deleterious for cell regulation, uracil must be removed from DNA. The major pathway of its repair is initiated by uracil-DNA gly ... Full text Cite

Ionic strength and magnesium affect the specificity of Escherichia coli and human 8-oxoguanine-DNA glycosylases.

Journal Article The FEBS journal · August 2008 An abundant oxidative lesion, 8-oxo-7,8-dihydroguanine (8-oxoG), often directs the misincorporation of dAMP during replication. To prevent mutations, cells possess an enzymatic system for the removal of 8-oxoG. A key element of this system is 8-oxoguanine- ... Full text Cite

Correlated cleavage of single- and double-stranded substrates by uracil-DNA glycosylase.

Journal Article FEBS letters · February 2008 Uracil-DNA glycosylase (Ung) can quickly locate uracil bases in an excess of undamaged DNA. DNA glycosylases may use diffusion along DNA to facilitate lesion search, resulting in processivity, the ability of glycosylases to excise closely spaced lesions wi ... Full text Cite