Masayuki Onishi

Journal article Frontiers in cell and developmental biology · January 2024 Featured Publication Septins are a family of membrane-associated cytoskeletal guanine-nucleotide binding proteins that play crucial roles in various cellular processes, such as cell division, phagocytosis, and organelle fission. Despite their importance, the evolutionary origi ... Full text Cite

Insights into coral bleaching under heat stress from analysis of gene expression in a sea anemone model system.

Journal article Proceedings of the National Academy of Sciences of the United States of America · November 2020 Featured Publication Loss of endosymbiotic algae ("bleaching") under heat stress has become a major problem for reef-building corals worldwide. To identify genes that might be involved in triggering or executing bleaching, or in protecting corals from it, we used RNAseq to ana ... Full text Cite

Cleavage-furrow formation without F-actin in Chlamydomonas.

Journal article Proceedings of the National Academy of Sciences of the United States of America · August 2020 Featured Publication It is widely believed that cleavage-furrow formation during cytokinesis is driven by the contraction of a ring containing F-actin and type-II myosin. However, even in cells that have such rings, they are not always essential for furrow formation. Moreover, ... Full text Cite

F-actin homeostasis through transcriptional regulation and proteasome-mediated proteolysis.

Journal article Proceedings of the National Academy of Sciences of the United States of America · July 2018 Featured Publication Many organisms possess multiple and often divergent actins whose regulation and roles are not understood in detail. For example, Chlamydomonas reinhardtii has both a conventional actin (IDA5) and a highly divergent one (NAP1); only IDA5 is expressed ... Full text Cite

Role of the Hof1-Cyk3 interaction in cleavage-furrow ingression and primary-septum formation during yeast cytokinesis.

Journal article Molecular biology of the cell · March 2018 Featured Publication In Saccharomyces cerevisiae, it is well established that Hof1, Cyk3, and Inn1 contribute to septum formation and cytokinesis. Because hof1∆ and cyk3∆ single mutants have relatively mild defects but hof1∆ cyk3∆ double mutants are ... Full text Cite

Evidence That an Unconventional Actin Can Provide Essential F-Actin Function and That a Surveillance System Monitors F-Actin Integrity in Chlamydomonas.

Journal article Genetics · March 2016 Featured Publication Actin is one of the most conserved eukaryotic proteins. It is thought to have multiple essential cellular roles and to function primarily or exclusively as filaments ("F-actin"). Chlamydomonas has been an enigma, because a null mutation (ida5-1) in its sin ... Full text Cite

The nonopisthokont septins: How many there are, how little we know about them, and how we might learn more.

Book section · January 2016 Featured Publication We have confirmed and extended previous reports of a wide distribution of septin proteins in the eukaryotic phylogeny. It now appears that septins are present in at least some representatives of every eukaryotic supergroup, with the possible exception of t ... Full text Cite

Distinct roles of Rho1, Cdc42, and Cyk3 in septum formation and abscission during yeast cytokinesis.

Journal article The Journal of cell biology · July 2013 Featured Publication In yeast and animal cytokinesis, the small guanosine triphosphatase (GTPase) Rho1/RhoA has an established role in formation of the contractile actomyosin ring, but its role, if any, during cleavage-furrow ingression and abscission is poorly understood. Thr ... Full text Cite

Role of septins in the orientation of forespore membrane extension during sporulation in fission yeast.

Journal article Molecular and cellular biology · April 2010 Featured Publication During yeast sporulation, a forespore membrane (FSM) initiates at each spindle-pole body and extends to form the spore envelope. We used Schizosaccharomyces pombe to investigate the role of septins during this process. During the prior conjugation of haplo ... Full text Cite

Role of Inn1 and its interactions with Hof1 and Cyk3 in promoting cleavage furrow and septum formation in S. cerevisiae.

Journal article The Journal of cell biology · June 2009 Featured Publication Cytokinesis requires coordination of actomyosin ring (AMR) contraction with rearrangements of the plasma membrane and extracellular matrix. In Saccharomyces cerevisiae, new membrane, the chitin synthase Chs2 (which forms the primary septum [PS]), and the p ... Full text Cite