Scholars@Duke
Masayuki Onishi

Masayuki Onishi

Assistant Professor of Biology
Biology
130 Science Dr., Box 90338, Durham, NC 27708
124 Science Dr., FFSC 3105, Durham, NC 27708

Scholarly Works - Preprints

Testing Reversibility of Endosymbiotic Gene Transfer between Chloroplast and Nucleus

Preprint · July 6, 2026 ABSTRACT Most proteins targeted to the organelles of endosymbiotic origin are encoded in the nuclear genome, placing them under the regulatory dominance of the nucleus. For photosynthetic ... Full text Cite

<p><span>MITH1 Promotes Biased Positioning of Chloroplast Nucleoids Linked to Localized rbcL Expression and Pyrenoid Biogenesis in Chlamydomonas reinhardtii</span></p>

Preprint · 2026 Chloroplast nucleoids are frequently associated with pyrenoids across diverse algal lineages, yet the significance of this conserved spatial relationship has remained unknown. Here, we show that chloroplast nucleoids are significantly and dynamical ... Full text Cite

Mitotic entry is controlled by the plant-specific phosphatase BSL1 and cyclin-dependent kinase B

Preprint · 2025 Cell cycle regulation is well understood in opisthokonts (fungi and metazoans) but not in plants and Apicomplexa, as some cell cycle regulators are not conserved 1–3 . In opisthokonts, cell cycle progression requires dephosphorylation of cyclin-dependent ... Full text Cite

Tubulin Acetylation Deficiency Promotes Axonemal Turnover and Increases Cytoplasmic Microtubules

Preprint · 2025 Tubulin post-translational modifications regulate microtubule dynamics; among these, α-tubulin acetylation has been linked to microtubule stability. We generated a Chlamydomonas mutant lacking the acetyltransferase αTAT1, which completely abolished α-tubu ... Full text Cite

Intraspecific reaction norm variation directs eco-evolutionary responses to environmental change

Preprint · September 3, 2024 As environmental change accelerates globally, understanding concurrent organismal, species, and community responses is increasingly vital. Here, we examine these coordinated responses by incorporating genotype-specific reaction norms into an eco-ev ... Full text Cite