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Tatiana Segura

Professor of Biomedical Engineering
Biomedical Engineering
534 Research Drive, Durham, NC 27708

Selected Publications


Optimizing neurotransmitter pathway detection by IR-MALDESI-MSI in mouse brain.

Journal Article Analytical and bioanalytical chemistry · July 2024 Mass spectrometry imaging (MSI) platforms such as infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) are advantageous for a variety of applications, including elucidating the localization of neurotransmitters (NTs) and related m ... Full text Cite

Engaging natural antibody responses for the treatment of inflammatory bowel disease via phosphorylcholine-presenting nanofibres.

Journal Article Nat Biomed Eng · May 2024 Inflammatory bowel disease lacks a long-lasting and broadly effective therapy. Here, by taking advantage of the anti-infection and anti-inflammatory properties of natural antibodies against the small-molecule epitope phosphorylcholine (PC), we show in mult ... Full text Link to item Cite

Gene Delivery From Granular Scaffolds for Tunable Biologics Manufacturing.

Journal Article Small · March 11, 2024 The understanding of the molecular basis for disease has generated a myriad of therapeutic biologics, including therapeutic proteins, antibodies, and viruses. However, the promise that biologics can resolve currently incurable diseases hinges in their manu ... Full text Link to item Cite

Exploring the Role of Spatial Confinement in Immune Cell Recruitment and Regeneration of Skin Wounds.

Journal Article Advanced materials (Deerfield Beach, Fla.) · December 2023 Microporous annealed particle (MAP) scaffolds are injectable granular materials comprised of micron sized hydrogel particles (microgels). The diameter of these microgels directly determines the size of the interconnected void space between particles where ... Full text Cite

Identification and analysis of 3D pores in packed particulate materials

Journal Article Nature Computational Science · November 21, 2023 We took the classic ‘guess the number of beans in a jar game’ and amplified the research question. Rather than estimate the quantity of particles in the jar, we sought to characterize the spaces between them. Here we present an approach for delineating the ... Full text Cite

SDF-1 Bound Heparin Nanoparticles Recruit Progenitor Cells for Their Differentiation and Promotion of Angiogenesis after Stroke.

Journal Article Advanced healthcare materials · November 2023 Angiogenesis after stroke is correlated with enhanced tissue repair and functional outcomes. The existing body of research in biomaterials for stroke focuses on hydrogels for the delivery of stem cells, growth factors, or small molecules or drugs. Despite ... Full text Cite

Biology-driven material design for ischaemic stroke repair

Journal Article Nature Reviews Bioengineering · October 27, 2023 Ischaemic stroke remains a leading cause of disability, with current clinical treatment options mainly focusing on mitigating immediate damage. The limited self-renewing capacity of the brain hinders tissue regeneration and prevents full recovery for many ... Full text Cite

Spatial Confinement Modulates Macrophage Response in Microporous Annealed Particle (MAP) Scaffolds.

Journal Article Advanced healthcare materials · October 2023 Macrophages are essential in the initiation, maintenance, and transition of inflammatory processes such as foreign body response and wound healing. Mounting evidence suggests that physical factors also modulate macrophage activation. 2D in vitro systems de ... Full text Cite

Void Volume Fraction of Granular Scaffolds.

Journal Article Small (Weinheim an der Bergstrasse, Germany) · October 2023 Void volume fraction (VVF) is a global measurement frequently used to characterize the void space of granular scaffolds, yet there is no gold standard by which to measure VVF in practice. To study the relationship  between VVF and particles of varying size ... Full text Cite

SDF-1 Bound Heparin Nanoparticles Recruit Progenitor Cells for Their Differentiation and Promotion of Angiogenesis After Stroke.

Journal Article bioRxiv · July 5, 2023 Angiogenesis after stroke is correlated with enhanced tissue repair and functional outcomes. The existing body of research in biomaterials for stroke focuses on hydrogels for the delivery of stem cells, growth factors, or small molecules or drugs. Despite ... Full text Link to item Cite

Enhanced Detection of Charged N-Glycans in the Brain by Infrared Matrix-Assisted Laser Desorption Electrospray Ionization Mass Spectrometric Imaging.

Journal Article Analytical chemistry · July 2023 N-linked glycosylation represents a structurally diverse, complex, co- and posttranslational protein modification that bridges metabolism and cellular signaling. Consequently, aberrant protein glycosylation is a hallmark of most pathological scenari ... Full text Cite

Lipidomic Analysis of Mouse Brain to Evaluate the Efficacy and Preservation of Different Tissue Preparatory Techniques by IR-MALDESI-MSI.

Journal Article Journal of the American Society for Mass Spectrometry · May 2023 Numerous preparatory methods have been developed to preserve the cellular and structural integrity of various biological tissues for different -omics studies. Herein, two preparatory methods for mass spectrometry imaging (MSI) were evaluated, fresh-frozen ... Full text Cite

A Balance between Pro-Inflammatory and Pro-Reparative Macrophages is Observed in Regenerative D-MAPS.

Journal Article Advanced science (Weinheim, Baden-Wurttemberg, Germany) · April 2023 Microporous annealed particle scaffolds (MAPS) are a new class of granular materials generated through the interlinking of tunable microgels, which produce an interconnected network of void space. These microgel building blocks can be designed with differe ... Full text Cite

IL7 and IL7 Flt3L co-expressing CAR T cells improve therapeutic efficacy in mouse EGFRvIII heterogeneous glioblastoma.

Journal Article Front Immunol · 2023 Chimeric antigen receptor (CAR) T cell therapy in glioblastoma faces many challenges including insufficient CAR T cell abundance and antigen-negative tumor cells evading targeting. Unfortunately, preclinical studies evaluating CAR T cells in glioblastoma f ... Full text Link to item Cite

Nanoenhancer for improving naked DNA electrotransfection In vivo.

Journal Article Frontiers in bioengineering and biotechnology · January 2023 Introduction: Electrotransfection (ET) is a non-viral approach widely used for delivery of naked nucleic acids. Its efficiency can be increased in vitro by treatment of cells with various small molecule enhancers. However, these enhancers oft ... Full text Cite

Controlling Particle Fraction in Microporous Annealed Particle Scaffolds for 3D Cell Culture.

Journal Article Journal of visualized experiments : JoVE · October 2022 Microgels are the building blocks of microporous annealed particle (MAP) scaffolds, which serve as a platform for both in vitro cell culture and in vivo tissue repair. In these granular scaffolds, the innate porosity generated by the void space between mic ... Full text Cite

Particle fraction is a bioactive cue in granular scaffolds.

Journal Article Acta biomaterialia · September 2022 Microporous annealed particle (MAP) hydrogels are porous 3D scaffolds generated by interlinking randomly packed microgels (µgels). Particle fraction, hydrogel stiffness, microparticle shape, and crosslinking chemistry are paramount to the microstructure th ... Full text Cite

Stoichiometric Post-Modification of Hydrogel Microparticles Dictates Neural Stem Cell Fate in Microporous Annealed Particle Scaffolds.

Journal Article Advanced materials (Deerfield Beach, Fla.) · August 2022 Microporous annealed particle (MAP) scaffolds are generated from assembled hydrogel microparticles (microgels). It has been previously demonstrated that MAP scaffold are porous, biocompatible, and recruit neural progenitor cells (NPCs) to the stroke cavity ... Full text Cite

Particle hydrogels decrease cerebral atrophy and attenuate astrocyte and microglia/macrophage reactivity after stroke.

Journal Article Advanced therapeutics · August 2022 Increasing numbers of individuals live with stroke related disabilities. Following stroke, highly reactive astrocytes and pro-inflammatory microglia can release cytokines and lead to a cytotoxic environment that causes further brain damage and prevents end ... Full text Cite

Optimizing neurotransmitter pathway detection by IR-MALDESI-MSI in mouse brain.

Journal Article Analytical and bioanalytical chemistry · July 2024 Mass spectrometry imaging (MSI) platforms such as infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) are advantageous for a variety of applications, including elucidating the localization of neurotransmitters (NTs) and related m ... Full text Cite

Engaging natural antibody responses for the treatment of inflammatory bowel disease via phosphorylcholine-presenting nanofibres.

Journal Article Nat Biomed Eng · May 2024 Inflammatory bowel disease lacks a long-lasting and broadly effective therapy. Here, by taking advantage of the anti-infection and anti-inflammatory properties of natural antibodies against the small-molecule epitope phosphorylcholine (PC), we show in mult ... Full text Link to item Cite

Gene Delivery From Granular Scaffolds for Tunable Biologics Manufacturing.

Journal Article Small · March 11, 2024 The understanding of the molecular basis for disease has generated a myriad of therapeutic biologics, including therapeutic proteins, antibodies, and viruses. However, the promise that biologics can resolve currently incurable diseases hinges in their manu ... Full text Link to item Cite

Exploring the Role of Spatial Confinement in Immune Cell Recruitment and Regeneration of Skin Wounds.

Journal Article Advanced materials (Deerfield Beach, Fla.) · December 2023 Microporous annealed particle (MAP) scaffolds are injectable granular materials comprised of micron sized hydrogel particles (microgels). The diameter of these microgels directly determines the size of the interconnected void space between particles where ... Full text Cite

Identification and analysis of 3D pores in packed particulate materials

Journal Article Nature Computational Science · November 21, 2023 We took the classic ‘guess the number of beans in a jar game’ and amplified the research question. Rather than estimate the quantity of particles in the jar, we sought to characterize the spaces between them. Here we present an approach for delineating the ... Full text Cite

SDF-1 Bound Heparin Nanoparticles Recruit Progenitor Cells for Their Differentiation and Promotion of Angiogenesis after Stroke.

Journal Article Advanced healthcare materials · November 2023 Angiogenesis after stroke is correlated with enhanced tissue repair and functional outcomes. The existing body of research in biomaterials for stroke focuses on hydrogels for the delivery of stem cells, growth factors, or small molecules or drugs. Despite ... Full text Cite

Biology-driven material design for ischaemic stroke repair

Journal Article Nature Reviews Bioengineering · October 27, 2023 Ischaemic stroke remains a leading cause of disability, with current clinical treatment options mainly focusing on mitigating immediate damage. The limited self-renewing capacity of the brain hinders tissue regeneration and prevents full recovery for many ... Full text Cite

Spatial Confinement Modulates Macrophage Response in Microporous Annealed Particle (MAP) Scaffolds.

Journal Article Advanced healthcare materials · October 2023 Macrophages are essential in the initiation, maintenance, and transition of inflammatory processes such as foreign body response and wound healing. Mounting evidence suggests that physical factors also modulate macrophage activation. 2D in vitro systems de ... Full text Cite

Void Volume Fraction of Granular Scaffolds.

Journal Article Small (Weinheim an der Bergstrasse, Germany) · October 2023 Void volume fraction (VVF) is a global measurement frequently used to characterize the void space of granular scaffolds, yet there is no gold standard by which to measure VVF in practice. To study the relationship  between VVF and particles of varying size ... Full text Cite

SDF-1 Bound Heparin Nanoparticles Recruit Progenitor Cells for Their Differentiation and Promotion of Angiogenesis After Stroke.

Journal Article bioRxiv · July 5, 2023 Angiogenesis after stroke is correlated with enhanced tissue repair and functional outcomes. The existing body of research in biomaterials for stroke focuses on hydrogels for the delivery of stem cells, growth factors, or small molecules or drugs. Despite ... Full text Link to item Cite

Enhanced Detection of Charged N-Glycans in the Brain by Infrared Matrix-Assisted Laser Desorption Electrospray Ionization Mass Spectrometric Imaging.

Journal Article Analytical chemistry · July 2023 N-linked glycosylation represents a structurally diverse, complex, co- and posttranslational protein modification that bridges metabolism and cellular signaling. Consequently, aberrant protein glycosylation is a hallmark of most pathological scenari ... Full text Cite

Lipidomic Analysis of Mouse Brain to Evaluate the Efficacy and Preservation of Different Tissue Preparatory Techniques by IR-MALDESI-MSI.

Journal Article Journal of the American Society for Mass Spectrometry · May 2023 Numerous preparatory methods have been developed to preserve the cellular and structural integrity of various biological tissues for different -omics studies. Herein, two preparatory methods for mass spectrometry imaging (MSI) were evaluated, fresh-frozen ... Full text Cite

A Balance between Pro-Inflammatory and Pro-Reparative Macrophages is Observed in Regenerative D-MAPS.

Journal Article Advanced science (Weinheim, Baden-Wurttemberg, Germany) · April 2023 Microporous annealed particle scaffolds (MAPS) are a new class of granular materials generated through the interlinking of tunable microgels, which produce an interconnected network of void space. These microgel building blocks can be designed with differe ... Full text Cite

IL7 and IL7 Flt3L co-expressing CAR T cells improve therapeutic efficacy in mouse EGFRvIII heterogeneous glioblastoma.

Journal Article Front Immunol · 2023 Chimeric antigen receptor (CAR) T cell therapy in glioblastoma faces many challenges including insufficient CAR T cell abundance and antigen-negative tumor cells evading targeting. Unfortunately, preclinical studies evaluating CAR T cells in glioblastoma f ... Full text Link to item Cite

Nanoenhancer for improving naked DNA electrotransfection In vivo.

Journal Article Frontiers in bioengineering and biotechnology · January 2023 Introduction: Electrotransfection (ET) is a non-viral approach widely used for delivery of naked nucleic acids. Its efficiency can be increased in vitro by treatment of cells with various small molecule enhancers. However, these enhancers oft ... Full text Cite

Controlling Particle Fraction in Microporous Annealed Particle Scaffolds for 3D Cell Culture.

Journal Article Journal of visualized experiments : JoVE · October 2022 Microgels are the building blocks of microporous annealed particle (MAP) scaffolds, which serve as a platform for both in vitro cell culture and in vivo tissue repair. In these granular scaffolds, the innate porosity generated by the void space between mic ... Full text Cite

Particle fraction is a bioactive cue in granular scaffolds.

Journal Article Acta biomaterialia · September 2022 Microporous annealed particle (MAP) hydrogels are porous 3D scaffolds generated by interlinking randomly packed microgels (µgels). Particle fraction, hydrogel stiffness, microparticle shape, and crosslinking chemistry are paramount to the microstructure th ... Full text Cite

Stoichiometric Post-Modification of Hydrogel Microparticles Dictates Neural Stem Cell Fate in Microporous Annealed Particle Scaffolds.

Journal Article Advanced materials (Deerfield Beach, Fla.) · August 2022 Microporous annealed particle (MAP) scaffolds are generated from assembled hydrogel microparticles (microgels). It has been previously demonstrated that MAP scaffold are porous, biocompatible, and recruit neural progenitor cells (NPCs) to the stroke cavity ... Full text Cite

Particle hydrogels decrease cerebral atrophy and attenuate astrocyte and microglia/macrophage reactivity after stroke.

Journal Article Advanced therapeutics · August 2022 Increasing numbers of individuals live with stroke related disabilities. Following stroke, highly reactive astrocytes and pro-inflammatory microglia can release cytokines and lead to a cytotoxic environment that causes further brain damage and prevents end ... Full text Cite

Nucleic Acid Delivery from Granular Hydrogels.

Journal Article Advanced healthcare materials · February 2022 Nucleic acid delivery has applications ranging from tissue engineering to vaccine development to infectious disease. Cationic polymer condensed nucleic acids are used with surface-coated porous scaffolds and are able to promote long-term gene expression. H ... Full text Cite

Injectable biomaterial shuttles for cell therapy in stroke.

Journal Article Brain research bulletin · November 2021 Ischemic stroke (IS) is the leading cause of disability and contributes to a significant socio-economic cost in the western world. Brain repair strategies investigated in the pre-clinical models include the delivery of drug or cell-based therapeutics; whic ... Full text Cite

Point-of-care antimicrobial coating protects orthopaedic implants from bacterial challenge.

Journal Article Nature communications · September 2021 Implant related infections are the most common cause of joint arthroplasty failure, requiring revision surgeries and a new implant, resulting in a cost of $8.6 billion annually. To address this problem, we created a class of coating technology that is appl ... Full text Cite

Activating an adaptive immune response from a hydrogel scaffold imparts regenerative wound healing.

Journal Article Nature materials · April 2021 Microporous annealed particle (MAP) scaffolds are flowable, in situ crosslinked, microporous scaffolds composed of microgel building blocks and were previously shown to accelerate wound healing. To promote more extensive tissue ingrowth before scaffold deg ... Full text Open Access Cite

Wound healing with topical BRAF inhibitor therapy in a diabetic model suggests tissue regenerative effects.

Journal Article PloS one · January 2021 Wound healing is a multi-step process to rapidly restore the barrier function. This process is often impaired in diabetic patients resulting in chronic wounds and amputation. We previously found that paradoxical activation of the mitogen-activated protein ... Full text Cite

Injectable biomaterials for treatment of glioblastoma.

Journal Article Advanced materials interfaces · October 2020 Despite ongoing advancements in the field of medicine, glioblastoma multiforme (GBM) is presently incurable, making this advanced brain tumor the deadliest tumor type in the central nervous system. The primary treatment strategies for GBM (i.e. surgical re ... Full text Cite

Injection of Hydrogel Biomaterial Scaffolds to The Brain After Stroke.

Journal Article Journal of visualized experiments : JoVE · October 2020 Stroke is the leading cause of disability and the fifth-leading cause of death in the United States. Approximately 87% of all strokes are ischemic strokes and are defined as the sudden blockage of a vessel supplying blood to the brain. Within minutes of th ... Full text Cite

Rapid Fabrication of Membrane-Integrated Thermoplastic Elastomer Microfluidic Devices.

Journal Article Micromachines · July 2020 Leveraging the advantageous material properties of recently developed soft thermoplastic elastomer materials, this work presents the facile and rapid fabrication of composite membrane-integrated microfluidic devices consisting of FlexdymTM polym ... Full text Cite

Materials to Promote Recovery After Stroke.

Journal Article Current opinion in biomedical engineering · June 2020 Stroke is the leading cause of long-term disability with no current treatment addressing post-stroke disability. The complex pathophysiology of stroke and the brain's limited potential for regeneration prevents sufficient endogenous repair for complete rec ... Full text Open Access Cite

Click by Click Microporous Annealed Particle (MAP) Scaffolds.

Journal Article Advanced healthcare materials · May 2020 Macroporous scaffolds are being increasingly used in regenerative medicine and tissue repair. While the recently developed microporous annealed particle (MAP) scaffolds have overcome issues with injectability and in situ hydrogel formation, limitations wit ... Full text Cite

The Use of a Novel Antimicrobial Implant Coating In Vivo to Prevent Spinal Implant Infection.

Journal Article Spine · March 2020 Study designA controlled, interventional animal study.ObjectiveSpinal implant infection (SII) is a devastating complication. The objective of this study was to evaluate the efficacy of a novel implant coating that has both a passive antib ... Full text Cite

Hydrogel microparticles for biomedical applications.

Journal Article Nature reviews. Materials · January 2020 Hydrogel microparticles (HMPs) are promising for biomedical applications, ranging from the therapeutic delivery of cells and drugs to the production of scaffolds for tissue repair and bioinks for 3D printing. Biologics (cells and drugs) can be encapsulated ... Full text Cite

Biomaterials-Mediated Regulation of Macrophage Cell Fate.

Journal Article Frontiers in bioengineering and biotechnology · January 2020 Endogenous regeneration aims to rebuild and reinstate tissue function through enlisting natural self-repairing processes. Promoting endogenous regeneration by reducing tissue-damaging inflammatory responses while reinforcing self-resolving inflammatory pro ... Full text Open Access Cite

Granular hydrogels: emergent properties of jammed hydrogel microparticles and their applications in tissue repair and regeneration.

Journal Article Current opinion in biotechnology · December 2019 Granular hydrogels are emerging as a versatile and effective platform for tissue engineered constructs in regenerative medicine. The hydrogel microparticles (HMPs) that compose these materials exhibit particle jamming above a minimum packing fraction, whic ... Full text Cite

Citrullination of fibronectin alters integrin clustering and focal adhesion stability promoting stromal cell invasion.

Journal Article Matrix biology : journal of the International Society for Matrix Biology · September 2019 The extracellular matrix (ECM) microenvironment is increasingly implicated in the instruction of pathologically relevant cell behaviors, from aberrant transdifferentation to invasion and beyond. Indeed, pathologic ECMs possess a panoply of alterations that ... Full text Cite

Enhanced In Vivo Delivery of Stem Cells using Microporous Annealed Particle Scaffolds.

Journal Article Small (Weinheim an der Bergstrasse, Germany) · September 2019 Delivery to the proper tissue compartment is a major obstacle hampering the potential of cellular therapeutics for medical conditions. Delivery of cells within biomaterials may improve localization, but traditional and newer void-forming hydrogels must be ... Full text Open Access Cite

Microporous annealed particle hydrogel stiffness, void space size, and adhesion properties impact cell proliferation, cell spreading, and gene transfer.

Journal Article Acta biomaterialia · August 2019 Designing scaffolds for polyplex-mediated therapeutic gene delivery has a number of applications in regenerative medicine, such as for tissue repair after wounding or disease. Microporous annealed particle (MAP) hydrogels are an emerging class of porous bi ... Full text Cite

Pathways Governing Polyethylenimine Polyplex Transfection in Microporous Annealed Particle Scaffolds.

Journal Article Bioconjugate chemistry · February 2019 Gene delivery using injectable hydrogels can serve as a potential method for regulated tissue regeneration in wound healing. Our microporous annealed particle (MAP) hydrogel has been shown to promote cellular infiltration in both skin and brain wounds, whi ... Full text Cite

Surface- and Hydrogel-Mediated Delivery of Nucleic Acid Nanoparticles.

Journal Article Methods in molecular biology (Clifton, N.J.) · January 2019 Gene expression within a cell population can be directly altered through gene delivery approaches. Traditionally for nonviral delivery, plasmids or siRNA molecules, encoding or targeting the gene of interest, are packaged within nanoparticles. These nanopa ... Full text Cite

Controlled Release of Vancomycin and Tigecycline from an Orthopaedic Implant Coating Prevents Staphylococcus aureus Infection in an Open Fracture Animal Model.

Journal Article BioMed research international · January 2019 IntroductionTreatment of open fractures routinely involves multiple surgeries and delayed definitive fracture fixation because of concern for infection. If implants were made less susceptible to infection, a one-stage procedure with intramedullary ... Full text Open Access Cite

Injectable and spatially patterned microporous annealed particle (MAP) hydrogels for tissue repair applications

Journal Article Advanced Science · October 11, 2018 Spatially patterned hydrogels are becoming increasingly popular in the field of regenerative medicine and tissue repair because of their ability to guide cell infiltration and migration. However, postfabrication technologies are usually required to spatial ... Full text Link to item Cite

It's All in the Delivery: Designing Hydrogels for Cell and Non-viral Gene Therapies.

Journal Article Molecular therapy : the journal of the American Society of Gene Therapy · September 2018 Hydrogels provide a regenerative medicine platform with their ability to create an environment that supports transplanted or endogenous infiltrating cells and enables these cells to restore or replace the function of tissues lost to disease or trauma. Furt ... Full text Cite

Hyaluronic acid hydrogel scaffolds loaded with cationic niosomes for efficient non-viral gene delivery.

Journal Article RSC advances · September 2018 The lack of ideal non-viral gene carriers has motivated the combination of delivery systems and tissue-engineered scaffolds, which may offer relevant advantages such as enhanced stability and reduced toxicity. In this work, we evaluated a new combination b ... Full text Cite

Dual-function injectable angiogenic biomaterial for the repair of brain tissue following stroke.

Journal Article Nature materials · July 2018 Stroke is the primary cause of disability due to the brain's limited ability to regenerate damaged tissue. After stroke, an increased inflammatory and immune response coupled with severely limited angiogenesis and neuronal growth results in a stroke cavity ... Full text Cite

Accelerated wound healing by injectable star poly(ethylene glycol)-b-poly(propylene sulfide) scaffolds loaded with poorly water-soluble drugs.

Journal Article Journal of controlled release : official journal of the Controlled Release Society · July 2018 Injectable hydrogel matrices take the shape of a wound cavity and serve as scaffold for tissue repair and regeneration. Yet these materials are generally hydrophilic, limiting the incorporation of poorly water soluble, hydrophobic drugs. Here we show this ... Full text Cite

In situ forming injectable hydrogels for drug delivery and wound repair.

Journal Article Advanced drug delivery reviews · March 2018 Hydrogels have been utilized in regenerative applications for many decades because of their biocompatibility and similarity in structure to the native extracellular matrix. Initially, these materials were formed outside of the patient and implanted using i ... Full text Cite

Sustained Transgene Expression via Hydrogel-Mediated Gene Transfer Results from Multiple Transfection Events.

Journal Article ACS biomaterials science & engineering · March 2018 Sustained delivery of therapeutic genes in vitro and in vivo has a wide range of applications in studying biology and in developing therapies for treating disease or repairing tissue. Nonviral vectors such as cationic polymers still present promising appro ... Full text Cite

Hydrogels with precisely controlled integrin activation dictate vascular patterning and permeability

Journal Article Nature Materials · September 1, 2017 Integrin binding to bioengineered hydrogel scaffolds is essential for tissue regrowth and regeneration, yet not all integrin binding can lead to tissue repair. Here, we show that through engineering hydrogel materials to promote α3/α5β1 integrin binding, w ... Full text Cite

Hydrogels for brain repair after stroke: an emerging treatment option

Journal Article Current opinion in biotechnology · 2016 Cite

Particle hydrogels based on hyaluronic acid building blocks

Journal Article ACS Biomaterials Science & Engineering · 2016 Cite

Systematic evaluation of natural scaffolds in cutaneous wound healing

Journal Article Journal of Materials Chemistry B · January 1, 2015 Current strategies to improve wound healing are often created from multiple components that may include a scaffold, cells, and bioactive cues. Acellular natural hydrogels are an attractive approach since the material's intrinsic biological activity can be ... Full text Cite

Hydrogel-based nanocomposites of therapeutic proteins for tissue repair

Journal Article Current opinion in chemical engineering · 2014 Cite

Matrix-based gene delivery for tissue repair

Journal Article Current opinion in biotechnology · 2013 Cite

Transfection in the third dimension

Journal Article Integrative Biology · 2013 Cite

Surface-and hydrogel-mediated delivery of nucleic acid nanoparticles

Journal Article Nanotechnology for Nucleic Acid Delivery: Methods and Protocols · 2013 Cite

Extracellular matrix modulates non-viral gene transfer to mouse mesenchymal stem cells

Journal Article Soft Matter · February 7, 2012 Non-viral gene delivery is severely limited by its efficiency. Previous studies aiming to improve the efficiency of non-viral gene delivery have focused on improving the vector system, while the cellular microenvironment where the cells reside is only begi ... Full text Cite

Biocompatible hydrogels by oxime Click chemistry

Journal Article Biomacromolecules · 2012 Cite

Protein- Polymer Nanoparticles for Nonviral Gene Delivery

Journal Article Biomacromolecules · 2011 Cite

Synthesis of protein nano-conjugates for cancer therapy

Journal Article Nano Research · 2011 Cite

siRNA applications in nanomedicine

Journal Article Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology · 2010 Cite

Quantum-dot-decorated robust transductable bioluminescent nanocapsules

Journal Article Journal of the American Chemical Society · 2010 Cite

Formulations and Delivery Limitations of Nucleic-Acid-Based Therapies

Journal Article Handbook of Pharmaceutical Biotechnology · 2007 Cite

Materials for cell-triggered and efficient gene transfer

Journal Article Nanomedicine: Nanotechnology, Biology and Medicine · 2007 Cite

Controlled surface-associated delivery of genes and oligonucleotides

Other Biological Systems Engineering: Papers and Publications · 2006 Cite

Gene delivery through cell culture substrate adsorbed DNA complexes

Journal Article Biotechnology and bioengineering · 2005 Cite

Surface-tethered DNA complexes for enhanced gene delivery

Journal Article Bioconjugate Chemistry · 2002 Cite

Materials for non-viral gene delivery

Journal Article Annual Review of Materials Research · 2001 Cite