Journal ArticleAdvanced healthcare materials · October 2024
Angiogenesis after stroke is correlated with enhanced tissue repair and functional outcomes. The existing body of research in biomaterials for stroke focuses on hydrogels for the delivery of stem cells, growth factors, or small molecules or drugs. Despite ...
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Journal ArticleSmall · August 2024
The understanding of the molecular basis for disease has generated a myriad of therapeutic biologics, including therapeutic proteins, antibodies, and viruses. However, the promise that biologics can resolve currently incurable diseases hinges in their manu ...
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Journal ArticleAnalytical and bioanalytical chemistry · July 2024
Mass spectrometry imaging (MSI) platforms such as infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) are advantageous for a variety of applications, including elucidating the localization of neurotransmitters (NTs) and related m ...
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Journal ArticleNat Biomed Eng · May 2024
Inflammatory bowel disease lacks a long-lasting and broadly effective therapy. Here, by taking advantage of the anti-infection and anti-inflammatory properties of natural antibodies against the small-molecule epitope phosphorylcholine (PC), we show in mult ...
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Journal ArticleAdvanced materials (Deerfield Beach, Fla.) · December 2023
Microporous annealed particle (MAP) scaffolds are injectable granular materials comprised of micron sized hydrogel particles (microgels). The diameter of these microgels directly determines the size of the interconnected void space between particles where ...
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Journal ArticleNature Computational Science · November 21, 2023
We took the classic ‘guess the number of beans in a jar game’ and amplified the research question. Rather than estimate the quantity of particles in the jar, we sought to characterize the spaces between them. Here we present an approach for delineating the ...
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Journal ArticleNature Reviews Bioengineering · October 27, 2023
Ischaemic stroke remains a leading cause of disability, with current clinical treatment options mainly focusing on mitigating immediate damage. The limited self-renewing capacity of the brain hinders tissue regeneration and prevents full recovery for many ...
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Journal ArticleAdvanced healthcare materials · October 2023
Macrophages are essential in the initiation, maintenance, and transition of inflammatory processes such as foreign body response and wound healing. Mounting evidence suggests that physical factors also modulate macrophage activation. 2D in vitro systems de ...
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Journal ArticleSmall (Weinheim an der Bergstrasse, Germany) · October 2023
Void volume fraction (VVF) is a global measurement frequently used to characterize the void space of granular scaffolds, yet there is no gold standard by which to measure VVF in practice. To study the relationship between VVF and particles of varying size ...
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Journal ArticlebioRxiv · July 5, 2023
Angiogenesis after stroke is correlated with enhanced tissue repair and functional outcomes. The existing body of research in biomaterials for stroke focuses on hydrogels for the delivery of stem cells, growth factors, or small molecules or drugs. Despite ...
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Journal ArticleAnalytical chemistry · July 2023
N-linked glycosylation represents a structurally diverse, complex, co- and posttranslational protein modification that bridges metabolism and cellular signaling. Consequently, aberrant protein glycosylation is a hallmark of most pathological scenari ...
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Journal ArticleJournal of the American Society for Mass Spectrometry · May 2023
Numerous preparatory methods have been developed to preserve the cellular and structural integrity of various biological tissues for different -omics studies. Herein, two preparatory methods for mass spectrometry imaging (MSI) were evaluated, fresh-frozen ...
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Journal ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · April 2023
Microporous annealed particle scaffolds (MAPS) are a new class of granular materials generated through the interlinking of tunable microgels, which produce an interconnected network of void space. These microgel building blocks can be designed with differe ...
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Journal ArticleFront Immunol · 2023
Chimeric antigen receptor (CAR) T cell therapy in glioblastoma faces many challenges including insufficient CAR T cell abundance and antigen-negative tumor cells evading targeting. Unfortunately, preclinical studies evaluating CAR T cells in glioblastoma f ...
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Journal ArticleFrontiers in bioengineering and biotechnology · January 2023
Introduction: Electrotransfection (ET) is a non-viral approach widely used for delivery of naked nucleic acids. Its efficiency can be increased in vitro by treatment of cells with various small molecule enhancers. However, these enhancers oft ...
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Journal ArticleJournal of visualized experiments : JoVE · October 2022
Microgels are the building blocks of microporous annealed particle (MAP) scaffolds, which serve as a platform for both in vitro cell culture and in vivo tissue repair. In these granular scaffolds, the innate porosity generated by the void space between mic ...
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Journal ArticleActa biomaterialia · September 2022
Microporous annealed particle (MAP) hydrogels are porous 3D scaffolds generated by interlinking randomly packed microgels (µgels). Particle fraction, hydrogel stiffness, microparticle shape, and crosslinking chemistry are paramount to the microstructure th ...
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Journal ArticleAdvanced materials (Deerfield Beach, Fla.) · August 2022
Microporous annealed particle (MAP) scaffolds are generated from assembled hydrogel microparticles (microgels). It has been previously demonstrated that MAP scaffold are porous, biocompatible, and recruit neural progenitor cells (NPCs) to the stroke cavity ...
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Journal ArticleAdvanced therapeutics · August 2022
Increasing numbers of individuals live with stroke related disabilities. Following stroke, highly reactive astrocytes and pro-inflammatory microglia can release cytokines and lead to a cytotoxic environment that causes further brain damage and prevents end ...
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Journal ArticleAdvanced healthcare materials · February 2022
Nucleic acid delivery has applications ranging from tissue engineering to vaccine development to infectious disease. Cationic polymer condensed nucleic acids are used with surface-coated porous scaffolds and are able to promote long-term gene expression. H ...
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Journal ArticleBrain research bulletin · November 2021
Ischemic stroke (IS) is the leading cause of disability and contributes to a significant socio-economic cost in the western world. Brain repair strategies investigated in the pre-clinical models include the delivery of drug or cell-based therapeutics; whic ...
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Journal ArticleNature communications · September 2021
Implant related infections are the most common cause of joint arthroplasty failure, requiring revision surgeries and a new implant, resulting in a cost of $8.6 billion annually. To address this problem, we created a class of coating technology that is appl ...
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Journal ArticleNature materials · April 2021
Microporous annealed particle (MAP) scaffolds are flowable, in situ crosslinked, microporous scaffolds composed of microgel building blocks and were previously shown to accelerate wound healing. To promote more extensive tissue ingrowth before scaffold deg ...
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Journal ArticlePloS one · January 2021
Wound healing is a multi-step process to rapidly restore the barrier function. This process is often impaired in diabetic patients resulting in chronic wounds and amputation. We previously found that paradoxical activation of the mitogen-activated protein ...
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Journal ArticleAdvanced materials interfaces · October 2020
Despite ongoing advancements in the field of medicine, glioblastoma multiforme (GBM) is presently incurable, making this advanced brain tumor the deadliest tumor type in the central nervous system. The primary treatment strategies for GBM (i.e. surgical re ...
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Journal ArticleJournal of visualized experiments : JoVE · October 2020
Stroke is the leading cause of disability and the fifth-leading cause of death in the United States. Approximately 87% of all strokes are ischemic strokes and are defined as the sudden blockage of a vessel supplying blood to the brain. Within minutes of th ...
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Journal ArticleMicromachines · July 2020
Leveraging the advantageous material properties of recently developed soft thermoplastic elastomer materials, this work presents the facile and rapid fabrication of composite membrane-integrated microfluidic devices consisting of FlexdymTM polym ...
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Journal ArticleCurrent opinion in biomedical engineering · June 2020
Stroke is the leading cause of long-term disability with no current treatment addressing post-stroke disability. The complex pathophysiology of stroke and the brain's limited potential for regeneration prevents sufficient endogenous repair for complete rec ...
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Journal ArticleAdvanced healthcare materials · May 2020
Macroporous scaffolds are being increasingly used in regenerative medicine and tissue repair. While the recently developed microporous annealed particle (MAP) scaffolds have overcome issues with injectability and in situ hydrogel formation, limitations wit ...
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Journal ArticleSpine · March 2020
Study designA controlled, interventional animal study.ObjectiveSpinal implant infection (SII) is a devastating complication. The objective of this study was to evaluate the efficacy of a novel implant coating that has both a passive antib ...
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Journal ArticleNature reviews. Materials · January 2020
Hydrogel microparticles (HMPs) are promising for biomedical applications, ranging from the therapeutic delivery of cells and drugs to the production of scaffolds for tissue repair and bioinks for 3D printing. Biologics (cells and drugs) can be encapsulated ...
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Journal ArticleFrontiers in bioengineering and biotechnology · January 2020
Endogenous regeneration aims to rebuild and reinstate tissue function through enlisting natural self-repairing processes. Promoting endogenous regeneration by reducing tissue-damaging inflammatory responses while reinforcing self-resolving inflammatory pro ...
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Journal ArticleCurrent opinion in biotechnology · December 2019
Granular hydrogels are emerging as a versatile and effective platform for tissue engineered constructs in regenerative medicine. The hydrogel microparticles (HMPs) that compose these materials exhibit particle jamming above a minimum packing fraction, whic ...
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Journal ArticleMatrix biology : journal of the International Society for Matrix Biology · September 2019
The extracellular matrix (ECM) microenvironment is increasingly implicated in the instruction of pathologically relevant cell behaviors, from aberrant transdifferentation to invasion and beyond. Indeed, pathologic ECMs possess a panoply of alterations that ...
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Journal ArticleSmall (Weinheim an der Bergstrasse, Germany) · September 2019
Delivery to the proper tissue compartment is a major obstacle hampering the potential of cellular therapeutics for medical conditions. Delivery of cells within biomaterials may improve localization, but traditional and newer void-forming hydrogels must be ...
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Journal ArticleActa biomaterialia · August 2019
Designing scaffolds for polyplex-mediated therapeutic gene delivery has a number of applications in regenerative medicine, such as for tissue repair after wounding or disease. Microporous annealed particle (MAP) hydrogels are an emerging class of porous bi ...
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Journal ArticleBioconjugate chemistry · February 2019
Gene delivery using injectable hydrogels can serve as a potential method for regulated tissue regeneration in wound healing. Our microporous annealed particle (MAP) hydrogel has been shown to promote cellular infiltration in both skin and brain wounds, whi ...
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Journal ArticleMethods in molecular biology (Clifton, N.J.) · January 2019
Gene expression within a cell population can be directly altered through gene delivery approaches. Traditionally for nonviral delivery, plasmids or siRNA molecules, encoding or targeting the gene of interest, are packaged within nanoparticles. These nanopa ...
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Journal ArticleBioMed research international · January 2019
IntroductionTreatment of open fractures routinely involves multiple surgeries and delayed definitive fracture fixation because of concern for infection. If implants were made less susceptible to infection, a one-stage procedure with intramedullary ...
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Journal ArticleAdvanced Science · October 11, 2018
Spatially patterned hydrogels are becoming increasingly popular in the field of regenerative medicine and tissue repair because of their ability to guide cell infiltration and migration. However, postfabrication technologies are usually required to spatial ...
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Journal ArticleMolecular therapy : the journal of the American Society of Gene Therapy · September 2018
Hydrogels provide a regenerative medicine platform with their ability to create an environment that supports transplanted or endogenous infiltrating cells and enables these cells to restore or replace the function of tissues lost to disease or trauma. Furt ...
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Journal ArticleRSC advances · September 2018
The lack of ideal non-viral gene carriers has motivated the combination of delivery systems and tissue-engineered scaffolds, which may offer relevant advantages such as enhanced stability and reduced toxicity. In this work, we evaluated a new combination b ...
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Journal ArticleNature materials · July 2018
Stroke is the primary cause of disability due to the brain's limited ability to regenerate damaged tissue. After stroke, an increased inflammatory and immune response coupled with severely limited angiogenesis and neuronal growth results in a stroke cavity ...
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Journal ArticleJournal of controlled release : official journal of the Controlled Release Society · July 2018
Injectable hydrogel matrices take the shape of a wound cavity and serve as scaffold for tissue repair and regeneration. Yet these materials are generally hydrophilic, limiting the incorporation of poorly water soluble, hydrophobic drugs. Here we show this ...
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Journal ArticleAdvanced drug delivery reviews · March 2018
Hydrogels have been utilized in regenerative applications for many decades because of their biocompatibility and similarity in structure to the native extracellular matrix. Initially, these materials were formed outside of the patient and implanted using i ...
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Journal ArticleACS biomaterials science & engineering · March 2018
Sustained delivery of therapeutic genes in vitro and in vivo has a wide range of applications in studying biology and in developing therapies for treating disease or repairing tissue. Nonviral vectors such as cationic polymers still present promising appro ...
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Journal ArticleNature Materials · September 1, 2017
Integrin binding to bioengineered hydrogel scaffolds is essential for tissue regrowth and regeneration, yet not all integrin binding can lead to tissue repair. Here, we show that through engineering hydrogel materials to promote α3/α5β1 integrin binding, w ...
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Journal ArticleJournal of Materials Chemistry B · January 1, 2015
Current strategies to improve wound healing are often created from multiple components that may include a scaffold, cells, and bioactive cues. Acellular natural hydrogels are an attractive approach since the material's intrinsic biological activity can be ...
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Journal ArticleSoft Matter · February 7, 2012
Non-viral gene delivery is severely limited by its efficiency. Previous studies aiming to improve the efficiency of non-viral gene delivery have focused on improving the vector system, while the cellular microenvironment where the cells reside is only begi ...
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