James Andrew Alspaugh II

Journal article Microbiology (Reading) · June 2004 Featured Work Many small G proteins require post-translational modification to allow functional association to the cell membrane. This process often involves the enzymic addition of hydrophobic prenyl groups to a conserved cysteine residue near the C-terminus of the pro ... Full text Link to item Cite

Cyclic AMP signaling in Cryptococcus neoformans.

Journal article FEMS Yeast Res · January 2004 Featured Work As pathogenic microorganisms establish an infection, they must be able to sense host-specific signals and respond by elaborating determinants that allow for survival in these hostile conditions. Pathogen cell surface proteins detect these signals and activ ... Full text Link to item Cite

Adenylyl cyclase functions downstream of the Galpha protein Gpa1 and controls mating and pathogenicity of Cryptococcus neoformans.

Journal article Eukaryot Cell · February 2002 Featured Work The signaling molecule cyclic AMP (cAMP) is a ubiquitous second messenger that enables cells to detect and respond to extracellular signals. cAMP is generated by the enzyme adenylyl cyclase, which is activated or inhibited by the Galpha subunits of heterot ... Full text Link to item Cite

Ras1 and Ras2 contribute shared and unique roles in physiology and virulence of Cryptococcus neoformans.

Journal article Microbiology (Reading) · January 2002 Featured Work The Ras1 signal transduction pathway controls the ability of the pathogenic fungus Cryptococcus neoformans to grow at high temperatures and to mate. A second RAS gene was identified in this organism. RAS2 is expressed at a very low level compared to RAS1, ... Full text Link to item Cite

RAS1 regulates filamentation, mating and growth at high temperature of Cryptococcus neoformans.

Journal article Mol Microbiol · April 2000 Featured Work Cryptococcus neoformans is a basidiomycete yeast and opportunistic human pathogen of increasing clinical importance due to the increasing population of immunocompromised patients. To further investigate signal transduction cascades regulating fungal pathog ... Full text Link to item Cite

Molecular analysis of the Cryptococcus neoformans ADE2 gene, a selectable marker for transformation and gene disruption.

Journal article Fungal Genet Biol · June 1999 Featured Work Cryptococcus neoformans is an important fungal pathogen of man. The incidence of cryptococcal disease has increased dramatically in patients immunocompromised because of HIV infection, organ transplantation, or treatment with cytotoxic chemotherapy or cort ... Full text Link to item Cite

Cryptococcus neoformans mating and virulence are regulated by the G-protein alpha subunit GPA1 and cAMP.

Journal article Genes Dev · December 1, 1997 Featured Work This study explores signal transduction pathways that function during mating and infection in the opportunistic, human fungal pathogen Cryptococcus neoformans. The gene encoding a G-protein alpha subunit homolog, GPA1, was disrupted by homologous recombina ... Full text Link to item Cite

Inhibition of Cryptococcus neoformans replication by nitrogen oxides supports the role of these molecules as effectors of macrophage-mediated cytostasis.

Journal article Infect Immun · July 1991 Featured Work Activated macrophages are able to inhibit the replication of intracellular microbes and tumor cells. In the murine system, this cytostatic effect is associated with the oxidation of L-arginine to L-citrulline, nitrite, and nitrate and is thought to be medi ... Full text Link to item Cite