Scholars@Duke
Ashutosh Chilkoti

Ashutosh Chilkoti

Alan L. Kaganov Distinguished Professor of Biomedical Engineering
Biomedical Engineering
Box 90281, Durham, NC 27708-0281
3381 CIEMAS, Durham, NC 27708

Featured Works

Inkjet-printed point-of-care immunoassay on a nanoscale polymer brush enables subpicomolar detection of analytes in blood.

Journal article Proc Natl Acad Sci U S A · August 22, 2017 Featured Publication The ELISA is the mainstay for sensitive and quantitative detection of protein analytes. Despite its utility, ELISA is time-consuming, resource-intensive, and infrastructure-dependent, limiting its availability in resource-limited regions. Here, we describe ... Full text Link to item Cite

One-week glucose control via zero-order release kinetics from an injectable depot of glucagon-like peptide-1 fused to a thermosensitive biopolymer.

Journal article Nat Biomed Eng · 2017 Featured Publication Stimulation of the glucagon-like peptide-1 (GLP1) receptor is a useful treatment strategy for type 2 diabetes because of pleiotropic effects, including the regulation of islet hormones and the induction of satiety. However, the native ligand for the GLP1 r ... Full text Link to item Cite

Combinatorial codon scrambling enables scalable gene synthesis and amplification of repetitive proteins.

Journal article Nature materials · April 2016 Featured Publication Most genes are synthesized using seamless assembly methods that rely on the polymerase chain reaction (PCR). However, PCR of genes encoding repetitive proteins either fails or generates nonspecific products. Motivated by the need to efficiently generate ne ... Full text Cite

A brush-polymer conjugate of exendin-4 reduces blood glucose for up to five days and eliminates poly(ethylene glycol) antigenicity.

Journal article Nat Biomed Eng · 2016 Featured Publication The delivery of therapeutic peptides and proteins is often challenged by a short half-life, and thus the need for frequent injections that limit efficacy, reduce patient compliance and increase treatment cost. Here, we demonstrate that a single subcutaneou ... Full text Link to item Cite

Sequence heuristics to encode phase behaviour in intrinsically disordered protein polymers.

Journal article Nature materials · November 2015 Featured Publication Proteins and synthetic polymers that undergo aqueous phase transitions mediate self-assembly in nature and in man-made material systems. Yet little is known about how the phase behaviour of a protein is encoded in its amino acid sequence. Here, by synthesi ... Full text Cite

A paclitaxel-loaded recombinant polypeptide nanoparticle outperforms Abraxane in multiple murine cancer models.

Journal article Nat Commun · August 4, 2015 Featured Publication Packaging clinically relevant hydrophobic drugs into a self-assembled nanoparticle can improve their aqueous solubility, plasma half-life, tumour-specific uptake and therapeutic potential. To this end, here we conjugated paclitaxel (PTX) to recombinant chi ... Full text Link to item Cite

A highly parallel method for synthesizing DNA repeats enables the discovery of 'smart' protein polymers.

Journal article Nat Mater · February 2011 Featured Publication Robust high-throughput synthesis methods are needed to expand the repertoire of repetitive protein-polymers for different applications. To address this need, we developed a new method, overlap extension rolling circle amplification (OERCA), for the highly ... Full text Link to item Cite

Purification of recombinant proteins by fusion with thermally-responsive polypeptides.

Journal article Nat Biotechnol · November 1999 Featured Publication Elastin-like polypeptides (ELPs) undergo a reversible, inverse phase transition. Below their transition temperature (Tt), ELPs are soluble in water, but when the temperature is raised above Tt, phase transition occurs, leading to aggregation of the polypep ... Full text Link to item Cite