Skip to main content

Bernard Mathey-Prevot

Adjunct Professor in the Department of Pharmacology & Cancer Biology
Pharmacology & Cancer Biology
Duke Box 3813, Durham, NC 27710
LSRC C104A, Durham, NC 27710

Selected Publications


Kinome screen of ferroptosis reveals a novel role of ATM in regulating iron metabolism.

Journal Article Cell Death Differ · March 2020 Ferroptosis is a specialized iron-dependent cell death that is associated with lethal lipid peroxidation. Modulation of ferroptosis may have therapeutic potential since it has been implicated in various human diseases as well as potential antitumor activit ... Full text Open Access Link to item Cite

Quantifying E2F1 protein dynamics in single cells.

Journal Article Quant Biol · March 2020 BACKGROUND: E2F1 protein, a major effector of the Rb/E2F pathway plays a central role in regulating cell-fate decisions involved in proliferation, apoptosis, and differentiation. Its expression is highly dynamic and tightly modulated through a combination ... Full text Link to item Cite

Author Correction: Division of labour between Myc and G1 cyclins in cell cycle commitment and pace control.

Journal Article Nat Commun · November 13, 2018 This Article contains errors in Supplementary Table 3, which are described in the Author Correction associated with this Article. The simulation results in the Article were based on the correct formula and thus the results are not affected by this correcti ... Full text Link to item Cite

CoA synthase regulates mitotic fidelity via CBP-mediated acetylation.

Conference Nat Commun · March 12, 2018 The temporal activation of kinases and timely ubiquitin-mediated degradation is central to faithful mitosis. Here we present evidence that acetylation controlled by Coenzyme A synthase (COASY) and acetyltransferase CBP constitutes a novel mechanism that en ... Full text Open Access Link to item Cite

Cyclin D/CDK4/6 activity controls G1 length in mammalian cells.

Journal Article PLoS One · 2018 The length of the G1 phase in the cell cycle shows significant variability in different cell types and tissue types. To gain insights into the control of G1 length, we generated an E2F activity reporter that captures free E2F activity after dissociation fr ... Full text Link to item Cite

Division of labour between Myc and G1 cyclins in cell cycle commitment and pace control.

Journal Article Nat Commun · September 1, 2014 A body of evidence has shown that the control of E2F transcription factor activity is critical for determining cell cycle entry and cell proliferation. However, an understanding of the precise determinants of this control, including the role of other cell- ... Full text Link to item Cite

Cross-platform prediction of gene expression signatures.

Journal Article PLoS One · 2013 Gene expression signatures can predict the activation of oncogenic pathways and other phenotypes of interest via quantitative models that combine the expression levels of multiple genes. However, as the number of platforms to measure genome-wide gene expre ... Full text Link to item Cite

FlyRNAi.org--the database of the Drosophila RNAi screening center: 2012 update.

Journal Article Nucleic Acids Res · January 2012 FlyRNAi (http://www.flyrnai.org), the database and website of the Drosophila RNAi Screening Center (DRSC) at Harvard Medical School, serves a dual role, tracking both production of reagents for RNA interference (RNAi) screening in Drosophila cells and RNAi ... Full text Link to item Cite

Network calisthenics: control of E2F dynamics in cell cycle entry.

Journal Article Cell Cycle · September 15, 2011 Stimulation of quiescent mammalian cells with mitogens induces an abrupt increase in E2F1-3 expression just prior to the onset of DNA synthesis, followed by a rapid decline as replication ceases. This temporal adaptation in E2F facilitates a transient patt ... Full text Link to item Cite

A pathway-based classification of human breast cancer.

Journal Article Proc Natl Acad Sci U S A · April 13, 2010 The hallmark of human cancer is heterogeneity, reflecting the complexity and variability of the vast array of somatic mutations acquired during oncogenesis. An ability to dissect this heterogeneity, to identify subgroups that represent common mechanisms of ... Full text Link to item Cite

Do-it-yourself RNAi made easy?

Journal Article Nat Methods · April 2007 Full text Link to item Cite

A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila.

Journal Article Genome Biol · 2007 Off-target effects have been demonstrated to be a major source of false-positives in RNA interference (RNAi) high-throughput screens. In this study, we re-assess the previously published transcriptional reporter-based whole-genome RNAi screens for the Wing ... Full text Link to item Cite

Matter arising: off-targets and genome-scale RNAi screens in Drosophila.

Journal Article Fly (Austin) · 2007 Recently, the issue of off-target effects (OTEs) associated with long double stranded RNAs (dsRNAs) used in RNAi screens, such as those performed at the Drosophila RNAi Screening Center and other laboratories, has become a focus of great interest and some ... Full text Link to item Cite

Design and implementation of high-throughput RNAi screens in cultured Drosophila cells.

Journal Article Nat Protoc · 2007 This protocol describes the various steps and considerations involved in planning and carrying out RNA interference (RNAi) genome-wide screens in cultured Drosophila cells. We focus largely on the procedures that have been modified as a result of our exper ... Full text Link to item Cite

Applications of high-throughput RNA interference screens to problems in cell and developmental biology.

Journal Article Genetics · January 2007 RNA interference (RNAi) in tissue culture cells has emerged as an excellent methodology for identifying gene functions systematically and in an unbiased manner. Here, we describe how RNAi high-throughput screening (HTS) in Drosophila cells are currently be ... Full text Link to item Cite

Drug-target identification in Drosophila cells: combining high-throughout RNAi and small-molecule screens.

Journal Article Drug Discov Today · January 2007 RNA interference (RNAi) and small-molecule approaches are synergistic on multiple levels, from technology and high-throughput screen development to target identification and functional studies. Here, we describe the RNAi screening platform that we have est ... Full text Link to item Cite

Evidence of off-target effects associated with long dsRNAs in Drosophila melanogaster cell-based assays.

Journal Article Nat Methods · October 2006 To evaluate the specificity of long dsRNAs used in high-throughput RNA interference (RNAi) screens performed at the Drosophila RNAi Screening Center (DRSC), we performed a global analysis of their activity in 30 genome-wide screens completed at our facilit ... Full text Link to item Cite

Minimizing the risk of reporting false positives in large-scale RNAi screens.

Journal Article Nat Methods · October 2006 Large-scale RNA interference (RNAi)-based analyses, very much as other 'omic' approaches, have inherent rates of false positives and negatives. The variability in the standards of care applied to validate results from these studies, if left unchecked, coul ... Full text Link to item Cite

Drosophila genome-wide RNAi screens: are they delivering the promise?

Journal Article Cold Spring Harb Symp Quant Biol · 2006 The emergence of RNA interference (RNAi) on the heels of the successful completion of the Drosophila genome project was seen by many as the ace in functional genomics: Its application would quickly assign a function to all genes in this organism and help d ... Full text Link to item Cite

Kinome screen of ferroptosis reveals a novel role of ATM in regulating iron metabolism.

Journal Article Cell Death Differ · March 2020 Ferroptosis is a specialized iron-dependent cell death that is associated with lethal lipid peroxidation. Modulation of ferroptosis may have therapeutic potential since it has been implicated in various human diseases as well as potential antitumor activit ... Full text Open Access Link to item Cite

Quantifying E2F1 protein dynamics in single cells.

Journal Article Quant Biol · March 2020 BACKGROUND: E2F1 protein, a major effector of the Rb/E2F pathway plays a central role in regulating cell-fate decisions involved in proliferation, apoptosis, and differentiation. Its expression is highly dynamic and tightly modulated through a combination ... Full text Link to item Cite

Author Correction: Division of labour between Myc and G1 cyclins in cell cycle commitment and pace control.

Journal Article Nat Commun · November 13, 2018 This Article contains errors in Supplementary Table 3, which are described in the Author Correction associated with this Article. The simulation results in the Article were based on the correct formula and thus the results are not affected by this correcti ... Full text Link to item Cite

CoA synthase regulates mitotic fidelity via CBP-mediated acetylation.

Conference Nat Commun · March 12, 2018 The temporal activation of kinases and timely ubiquitin-mediated degradation is central to faithful mitosis. Here we present evidence that acetylation controlled by Coenzyme A synthase (COASY) and acetyltransferase CBP constitutes a novel mechanism that en ... Full text Open Access Link to item Cite

Cyclin D/CDK4/6 activity controls G1 length in mammalian cells.

Journal Article PLoS One · 2018 The length of the G1 phase in the cell cycle shows significant variability in different cell types and tissue types. To gain insights into the control of G1 length, we generated an E2F activity reporter that captures free E2F activity after dissociation fr ... Full text Link to item Cite

Division of labour between Myc and G1 cyclins in cell cycle commitment and pace control.

Journal Article Nat Commun · September 1, 2014 A body of evidence has shown that the control of E2F transcription factor activity is critical for determining cell cycle entry and cell proliferation. However, an understanding of the precise determinants of this control, including the role of other cell- ... Full text Link to item Cite

Cross-platform prediction of gene expression signatures.

Journal Article PLoS One · 2013 Gene expression signatures can predict the activation of oncogenic pathways and other phenotypes of interest via quantitative models that combine the expression levels of multiple genes. However, as the number of platforms to measure genome-wide gene expre ... Full text Link to item Cite

FlyRNAi.org--the database of the Drosophila RNAi screening center: 2012 update.

Journal Article Nucleic Acids Res · January 2012 FlyRNAi (http://www.flyrnai.org), the database and website of the Drosophila RNAi Screening Center (DRSC) at Harvard Medical School, serves a dual role, tracking both production of reagents for RNA interference (RNAi) screening in Drosophila cells and RNAi ... Full text Link to item Cite

Network calisthenics: control of E2F dynamics in cell cycle entry.

Journal Article Cell Cycle · September 15, 2011 Stimulation of quiescent mammalian cells with mitogens induces an abrupt increase in E2F1-3 expression just prior to the onset of DNA synthesis, followed by a rapid decline as replication ceases. This temporal adaptation in E2F facilitates a transient patt ... Full text Link to item Cite

A pathway-based classification of human breast cancer.

Journal Article Proc Natl Acad Sci U S A · April 13, 2010 The hallmark of human cancer is heterogeneity, reflecting the complexity and variability of the vast array of somatic mutations acquired during oncogenesis. An ability to dissect this heterogeneity, to identify subgroups that represent common mechanisms of ... Full text Link to item Cite

Do-it-yourself RNAi made easy?

Journal Article Nat Methods · April 2007 Full text Link to item Cite

A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila.

Journal Article Genome Biol · 2007 Off-target effects have been demonstrated to be a major source of false-positives in RNA interference (RNAi) high-throughput screens. In this study, we re-assess the previously published transcriptional reporter-based whole-genome RNAi screens for the Wing ... Full text Link to item Cite

Matter arising: off-targets and genome-scale RNAi screens in Drosophila.

Journal Article Fly (Austin) · 2007 Recently, the issue of off-target effects (OTEs) associated with long double stranded RNAs (dsRNAs) used in RNAi screens, such as those performed at the Drosophila RNAi Screening Center and other laboratories, has become a focus of great interest and some ... Full text Link to item Cite

Design and implementation of high-throughput RNAi screens in cultured Drosophila cells.

Journal Article Nat Protoc · 2007 This protocol describes the various steps and considerations involved in planning and carrying out RNA interference (RNAi) genome-wide screens in cultured Drosophila cells. We focus largely on the procedures that have been modified as a result of our exper ... Full text Link to item Cite

Applications of high-throughput RNA interference screens to problems in cell and developmental biology.

Journal Article Genetics · January 2007 RNA interference (RNAi) in tissue culture cells has emerged as an excellent methodology for identifying gene functions systematically and in an unbiased manner. Here, we describe how RNAi high-throughput screening (HTS) in Drosophila cells are currently be ... Full text Link to item Cite

Drug-target identification in Drosophila cells: combining high-throughout RNAi and small-molecule screens.

Journal Article Drug Discov Today · January 2007 RNA interference (RNAi) and small-molecule approaches are synergistic on multiple levels, from technology and high-throughput screen development to target identification and functional studies. Here, we describe the RNAi screening platform that we have est ... Full text Link to item Cite

Evidence of off-target effects associated with long dsRNAs in Drosophila melanogaster cell-based assays.

Journal Article Nat Methods · October 2006 To evaluate the specificity of long dsRNAs used in high-throughput RNA interference (RNAi) screens performed at the Drosophila RNAi Screening Center (DRSC), we performed a global analysis of their activity in 30 genome-wide screens completed at our facilit ... Full text Link to item Cite

Minimizing the risk of reporting false positives in large-scale RNAi screens.

Journal Article Nat Methods · October 2006 Large-scale RNA interference (RNAi)-based analyses, very much as other 'omic' approaches, have inherent rates of false positives and negatives. The variability in the standards of care applied to validate results from these studies, if left unchecked, coul ... Full text Link to item Cite

Drosophila genome-wide RNAi screens: are they delivering the promise?

Journal Article Cold Spring Harb Symp Quant Biol · 2006 The emergence of RNA interference (RNAi) on the heels of the successful completion of the Drosophila genome project was seen by many as the ace in functional genomics: Its application would quickly assign a function to all genes in this organism and help d ... Full text Link to item Cite

FlyRNAi: the Drosophila RNAi screening center database.

Journal Article Nucleic Acids Res · January 1, 2006 RNA interference (RNAi) has become a powerful tool for genetic screening in Drosophila. At the Drosophila RNAi Screening Center (DRSC), we are using a library of over 21,000 double-stranded RNAs targeting known and predicted genes in Drosophila. This libra ... Full text Link to item Cite

High-throughput RNA interference screens in Drosophila tissue culture cells.

Journal Article Methods Enzymol · 2005 This chapter describes the method used to conduct high-throughput screening (HTs) by RNA interference in Drosophila tissue culture cells. It covers four main topics: (1) a brief description of the existing platforms to conduct RNAi-screens in cell-based as ... Full text Link to item Cite

Increased expression of Drosophila tetraspanin, Tsp68C, suppresses the abnormal proliferation of ytr-deficient and Ras/Raf-activated hemocytes.

Journal Article Oncogene · December 2, 2004 Tetraspanins are evolutionary conserved transmembrane proteins thought to facilitate cell proliferation, movement or fusion by acting as organizers of different signaling events. Despite their prevalence and conservation, their specific role and functions ... Full text Link to item Cite

Yantar, a conserved arginine-rich protein is involved in Drosophila hemocyte development.

Journal Article Dev Biol · September 1, 2004 To identify novel factors involved in Drosophila hematopoiesis, we screened a collection of lethal recessive mutations that also affected normal hemocyte composition in larvae. We present the characterization of the gene yantar (ytr) for which we isolated ... Full text Link to item Cite

Signaling role of hemocytes in Drosophila JAK/STAT-dependent response to septic injury.

Journal Article Dev Cell · September 2003 To characterize the features of JAK/STAT signaling in Drosophila immune response, we have identified totA as a gene that is regulated by the JAK/STAT pathway in response to septic injury. We show that septic injury triggers the hemocyte-specific expression ... Full text Link to item Cite

Differential requirement for STAT by gain-of-function and wild-type receptor tyrosine kinase Torso in Drosophila.

Journal Article Development · September 2002 Malignant transformation frequently involves aberrant signaling from receptor tyrosine kinases (RTKs). These receptors commonly activate Ras/Raf/MEK/MAPK signaling but when overactivated can also induce the JAK/STAT pathway, originally identified as the si ... Full text Link to item Cite

SOCS36E, a novel Drosophila SOCS protein, suppresses JAK/STAT and EGF-R signalling in the imaginal wing disc.

Journal Article Oncogene · July 18, 2002 We have cloned a novel SOCS gene from Drosophila, socs36E, which is most homologous to the mammalian socs-5 gene. Socs36E is expressed zygotically, predominantly during embryogenesis, in a highly dynamic pattern. In vivo expression of SOCS36E in transgenic ... Full text Link to item Cite

Functional genomic analysis of phagocytosis and identification of a Drosophila receptor for E. coli.

Journal Article Nature · April 11, 2002 The recognition and phagocytosis of microbes by macrophages is a principal aspect of innate immunity that is conserved from insects to humans. Drosophila melanogaster has circulating macrophages that phagocytose microbes similarly to mammalian macrophages, ... Full text Link to item Cite

Hydrophobic residues Phe751 and Leu753 are essential for STAT5 transcriptional activity.

Journal Article J Biol Chem · June 2, 2000 One facet of cytokine signaling is relayed to the nucleus by the activation, through tyrosine phosphorylation, of latent cytoplasmic signal transducers and activators of transcription (STAT) family members. It has been demonstrated that the C termini of ST ... Full text Link to item Cite

Rapid selection of tetracycline-controlled inducible cell lines using a green fluorescent-transactivator fusion protein.

Journal Article Biochem Biophys Res Commun · April 21, 1999 We describe a modification of the tetracycline-controlled expression system that facilitates the rapid identification of tetracycline-sensitive clones. The TetR/VP16 transactivator protein was tagged with the green fluorescent protein (GFP) at its N-termin ... Full text Link to item Cite

Interleukin-3-induced activation of the JAK/STAT pathway is prolonged by proteasome inhibitors.

Journal Article Blood · May 1, 1998 One facet of cytokine receptor signaling involves the activation of signal transducers and activators of transcription (STATs). STATs are rapidly activated via tyrosine phosphorylation by Janus kinase (JAK) family members and subsequently inactivated withi ... Link to item Cite

JAK2 is required for induction of the murine DUB-1 gene.

Journal Article Mol Cell Biol · June 1997 Cytokine receptors activate multiple signal transduction pathways, resulting in the induction of specific target genes. We have recently identified a hematopoietic cell-specific immediate-early gene, DUB-1, that encodes a growth-regulatory deubiquitinating ... Full text Link to item Cite

Receptors that induce erythroid differentiation of Ba/F3 cells: structural requirements and effect on STAT5 binding.

Journal Article Blood · May 1, 1997 Ectopic expression of the erythropoietin receptor (EpoR) in the interleukin-3 (IL-3)-dependent cell line Ba/F3 results in growth and partial erythroid differentiation in Epo. In contrast, introduction and activation of the interleukin-5 receptor (IL-5R) or ... Link to item Cite

The murine DUB-1 gene is specifically induced by the betac subunit of interleukin-3 receptor.

Journal Article Mol Cell Biol · September 1996 Cytokines regulate cell growth and differentiation by inducing the expression of specific target genes. We have recently isolated a cytokine-inducible, immediate-early cDNA, DUB-1, that encodes a deubiquitinating enzyme. The DUB-1 mRNA was specifically ind ... Full text Link to item Cite

Cooperation between core binding factor and adjacent promoter elements contributes to the tissue-specific expression of interleukin-3.

Journal Article J Biol Chem · June 14, 1996 Tissue-specific expression of interleukin-3 (IL-3) is mediated via cis-acting elements located within 315 base pairs of the transcription start. This is achieved in part through the positive activities of the AP-1 and Elf-1 sites in the IL-3 promoter. The ... Full text Link to item Cite

Multiple proteins interact with the nuclear inhibitory protein repressor element in the human interleukin-3 promoter.

Journal Article J Biol Chem · October 13, 1995 T cell expression of interleukin 3 (IL-3) is directed by positive and negative cis-acting DNA elements clustered within 300 base pairs of the transcriptional start site. A strong repressor element, termed nuclear inhibitory protein (NIP), was previously ma ... Full text Link to item Cite

Multiple transcription start sites and 5' alternate splicing of murine IL-3 receptor beta-chain transcripts.

Journal Article Biochem Biophys Res Commun · November 30, 1994 The murine interleukin-3 receptor beta-chain genes, IL-3R beta IL-3 and IL-3R beta c, encode the signal transducing chains of the high affinity receptors for IL-3 and IL-3, GM-CSF and IL-5 respectively. Little is known about the regulation of their express ... Full text Link to item Cite

Differentiation domains of the erythropoietin receptor.

Journal Article Proc Soc Exp Biol Med · July 1994 Ectopic expression of the erythropoietin receptor (Epo-R) in Ba/F3, an interleukin-3 (IL-3)-dependent progenitor cell line, confers both Epo-dependent cell growth and Epo-dependent induction of beta-globin mRNA. We have used this system of limited erythroi ... Full text Link to item Cite

Identification of a critical regulatory site in the human interleukin-3 promoter by in vivo footprinting.

Journal Article Blood · May 15, 1994 Interleukin-3 (IL-3) is involved in proliferation and differentiation of hematopoietic progenitor cells. Its expression is subject to precise, tissue-specific regulation, and has been studied extensively in the gibbon T-cell line MLA 144 by a combination o ... Link to item Cite

Erythropoietin receptor signals both proliferation and erythroid-specific differentiation.

Journal Article Proc Natl Acad Sci U S A · December 1, 1993 Ectopic expression of the erythropoietin receptor (EPO-R) in Ba/F3, an interleukin 3-dependent progenitor cell line, confers EPO-dependent cell growth. To examine whether the introduced EPO-R could affect differentiation, we isolated Ba/F3-EPO-R subclones ... Full text Link to item Cite

Interleukin-3 expression by activated T cells involves an inducible, T-cell-specific factor and an octamer binding protein.

Journal Article Blood · February 15, 1993 Interleukin-3 (IL-3) is exclusively expressed by activated T and natural killer cells, a function that is tightly controlled both in a lineage-specific and in a stimulation-dependent manner. We have investigated the protein binding characteristics and func ... Link to item Cite

Enhanced expression of interleukin-3 and granulocyte-macrophage colony-stimulating factor receptor subunits in murine hematopoietic cells stimulated with hematopoietic growth factors.

Journal Article Blood · September 1, 1992 AIC2A and AIC2B are closely related genes encoding components of the receptors for murine interleukin-3 (IL-3) (AIC2A) and granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-5 (AIC2B). We have studied the parallel regulation of expression of ... Link to item Cite

The erythropoietin receptor transmembrane region is necessary for activation by the Friend spleen focus-forming virus gp55 glycoprotein.

Journal Article Mol Cell Biol · July 1992 The erythropoietin receptor (EPO-R), a member of the cytokine receptor superfamily, can be activated by binding either erythropoietin (EPO) or gp55, the Friend spleen focus-forming virus glycoprotein. The highly specific interaction between gp55 and EPO-R ... Full text Link to item Cite

A functional isoform of the human granulocyte/macrophage colony-stimulating factor receptor has an unusual cytoplasmic domain.

Journal Article Proc Natl Acad Sci U S A · September 1, 1991 The granulocyte/macrophage colony-stimulating factor (GM-CSF) receptor (GMR) transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells. This study sought to determine whether functional isoform ... Full text Link to item Cite

Preneoplastic lesions induced by myc and src oncogenes in a heterotopic rat colon.

Journal Article Oncogene · February 1991 With the use of viral vectors harboring myc and src oncogenes, we have assessed the potential contribution of these different elements to colonic neoplasia using a transplantation technique resulting in the formation of a heterotopic colon in Wistar Furth ... Link to item Cite

Positive and negative elements regulate human interleukin 3 expression.

Journal Article Proc Natl Acad Sci U S A · July 1990 The human interleukin 3 (IL-3) promoter is comprised of several cis-acting DNA sequences that modulate T-cell expression of IL-3. These are located within 315 nucleotides upstream of the mRNA start site. Transient expression of reporter genes linked to ser ... Full text Link to item Cite

Granulocyte-macrophage colony-stimulating factor and interleukin-3 mRNAs are produced by a small fraction of blood mononuclear cells.

Journal Article Blood · October 1989 Northern blot analysis has identified granulocyte macrophage colony stimulating factor (GM-CSF) mRNA in monocytes and both GM-CSF and interleukin-3 (IL-3) mRNA in lymphocytes. However, these results have not addressed whether all cells or a subset of the p ... Link to item Cite

Expression of human interleukin-3 (multi-CSF) is restricted to human lymphocytes and T-cell tumor lines.

Journal Article Blood · March 1989 While the cellular sources for granulocyte-macrophage colony-stimulating factor (GM-CSF) are known to be widely distributed among several cell types, interleukin-3 (IL-3) gene expression has been demonstrated in only certain T-cell clones and in blood mono ... Link to item Cite

Regulation of the human interleukin-3 gene.

Journal Article Trans Assoc Am Physicians · 1989 Link to item Cite

A human immunoglobulin gene reduces the incidence of lymphomas in c-Myc-bearing transgenic mice.

Journal Article Nature · December 1, 1988 Transgenic mice carrying an immunoglobulin enhancer-driven c-myc oncogene develop rapid-onset pre-B cell lymphomas. The incidence of these malignancies is greatly reduced when an additional transgene encoding the membrane-bound form (but not the secreted f ... Full text Link to item Cite

Recombinants within the tyrosine kinase region of v-abl and v-src identify a v-abl segment that confers lymphoid specificity.

Journal Article Mol Cell Biol · January 1988 The v-abl and v-src oncogenes encode protein-tyrosine kinases that possess different biological properties in spite of their high degree of amino acid conservation. To correlate functional differences with structural domains of the two oncogenes, we recomb ... Full text Link to item Cite

Neuronal pp60c-src contains a six-amino acid insertion relative to its non-neuronal counterpart.

Journal Article Science · July 24, 1987 Neuronal cells express a pp60c-src variant that displays an altered electrophoretic mobility and a different V8 peptide pattern relative to pp60c-src expressed in tissues of non-neuronal origin. To determine whether the neuronal form of pp60c-src is encode ... Full text Link to item Cite

Abelson virus abrogation of interleukin-3 dependence in a lymphoid cell line.

Journal Article Mol Cell Biol · November 1986 Among several tyrosine-protein kinases, only v-abl could abrogate interleukin 3 dependence of a lymphoblastoid cell line; v-src and v-fps proteins gave partial or no interleukin 3 independence, respectively. Lymphokine independence was achieved via a nonau ... Full text Link to item Cite

Specific transforming potential of oncogenes encoding protein-tyrosine kinases.

Journal Article EMBO J · July 1985 Several chimeric murine retroviruses were constructed to test whether the gag sequence of Abelson murine leukemia virus (A-MuLV) could influence the in vitro specificity of two sarcoma-inducing oncogenes: src of Rous sarcoma virus and fps of Fujinami sarco ... Full text Link to item Cite

Preferential expression of the c-fps protein in chicken macrophages and granulocytic cells.

Journal Article Mol Cell Biol · May 1985 We have studied the expression of the protein kinase activity of NCP98, the c-fps gene product, in several hemopoietic tissues of chickens as a function of the developmental stage of these organs. We found that in bone marrow, spleen, and bursa, maximum NC ... Full text Link to item Cite

Revertants and partial transformants of rat fibroblasts infected with Fujinami sarcoma virus.

Journal Article J Virol · May 1984 Fifteen revertants were isolated from three independent clones of rat fibroblasts transformed by Fujinami sarcoma virus (FSV). Three revertant clones resulted from the deletion of the one copy of the FSV provirus, and one encoded an enzymatically inactive, ... Full text Link to item Cite

Isolation of 16L virus: a rapidly transforming sarcoma virus from an avian leukosis virus-induced sarcoma.

Journal Article Proc Natl Acad Sci U S A · August 1982 We have isolated a replication-defective rapidly transforming sarcoma virus (designated 16L virus) from a fibro-sarcoma in a chicken infected with td107A, a transformation-defective deletion mutant of subgroup A Schmidt-Ruppin Rous sarcoma virus. 16L virus ... Full text Link to item Cite

A cellular protein is immunologically crossreactive with and functionally homologous to the Fujinami sarcoma virus transforming protein.

Journal Article Cell · April 1982 We obtained a regressing-tumor antiserum specific for the unique sequence of the transforming protein P140 of Fujinami sarcoma virus by injecting Fischer rats with syngeneic embryo cells transformed with Fujinami sarcoma virus. This serum is capable of imm ... Full text Link to item Cite

Mutants of Fujinami sarcoma virus which are temperature sensitive for cellular transformation and protein kinase activity.

Journal Article J Virol · April 1981 Two temperature-sensitive mutants of Fujinami sarcoma virus were isolated and characterized. Cells infected with the mutants were temperature sensitive in focus formation, colony formation, increased sugar uptake, and synthesis of plasminogen activator. Th ... Full text Link to item Cite

Purification and chemical characterization of melittin and acetylated derivatives.

Journal Article Biochim Biophys Acta · October 21, 1980 Melittin, the main basic and hydrophobic peptide of bee venom, displays marked detergent-like properties. At high peptide concentration, and depending on salt and pH, it forms a tetramer. This is prevented by using urea. A purification procedure in presenc ... Full text Link to item Cite