Bruce Klitzman

Journal article Nano Research · August 1, 2018 Featured Publication Although nanotechnology has led to important advances in in vitro diagnostics, the development of nanosensors for in vivo detection remains very challenging. Here, we demonstrated the proof-of-principle of in vivo detection of nucleic acid targets using a ... Full text Open Access Cite

Antibiotic-induced changes in the microbiota disrupt redox dynamics in the gut.

Journal article Elife · June 19, 2018 Featured Publication How host and microbial factors combine to structure gut microbial communities remains incompletely understood. Redox potential is an important environmental feature affected by both host and microbial actions. We assessed how antibiotics, which can impact ... Full text Open Access Link to item Cite

Characterization of the Foreign Body Response to Common Surgical Biomaterials in a Murine Model.

Journal article Eur J Plast Surg · November 2017 Featured Publication BACKGROUND: Implanted biomaterials are subject to a significant reaction from the host, known as the foreign body response (FBR). We quantified the FBR to five materials following subcutaneous implantation in mice. MATERIALS AND METHODS: Polyvinyl alcohol ... Full text Open Access Link to item Cite

Injectable Phosphorescence-based Oxygen Biosensors Identify Post Ischemic Reactive Hyperoxia.

Journal article Sci Rep · August 15, 2017 Featured Publication Novel injectable biosensors were used to measure interstitial oxygenation before, during, and after transient ischemia. It is well known that reactive hyperemia occurs following a period of ischemia. However, increased blood flow does not necessarily mean ... Full text Open Access Link to item Cite

Stiffness of Protease Sensitive and Cell Adhesive PEG Hydrogels Promotes Neovascularization In Vivo.

Journal article Ann Biomed Eng · June 2017 Featured Publication Materials that support the assembly of new vasculature are critical for regenerative medicine. Controlling the scaffold's mechanical properties may help to optimize neovascularization within implanted biomaterials. However, reducing the stiffness of synthe ... Full text Open Access Link to item Cite

A medical-toxicological view of tattooing.

Journal article Lancet · January 23, 2016 Featured Publication Long perceived as a form of exotic self-expression in some social fringe groups, tattoos have left their maverick image behind and become mainstream, particularly for young people. Historically, tattoo-related health and safety regulations have focused on ... Full text Link to item Cite

Porous, Dexamethasone-loaded polyurethane coatings extend performance window of implantable glucose sensors in vivo.

Journal article Acta Biomater · January 2016 Featured Publication UNLABELLED: Continuous glucose sensors offer the promise of tight glycemic control for insulin dependent diabetics; however, utilization of such systems has been hindered by issues of tissue compatibility. Here we report on the in vivo performance of impla ... Full text Link to item Cite

In vivo detection of SERS-encoded plasmonic nanostars in human skin grafts and live animal models.

Journal article Anal Bioanal Chem · November 2015 Featured Publication Surface-enhanced Raman scattering (SERS)-active plasmonic nanomaterials have become a promising agent for molecular imaging and multiplex detection. Among the wide variety of plasmonics-active nanoparticles, gold nanostars offer unique plasmon properties t ... Full text Link to item Cite

Scaffold-free, Human Mesenchymal Stem Cell-Based Tissue Engineered Blood Vessels.

Journal article Sci Rep · October 12, 2015 Featured Publication Tissue-engineered blood vessels (TEBV) can serve as vascular grafts and may also play an important role in the development of organs-on-a-chip. Most TEBV construction involves scaffolding with biomaterials such as collagen gel or electrospun fibrous mesh. ... Full text Open Access Link to item Cite

In vitro and in vivo characterization of porous poly-L-lactic acid coatings for subcutaneously implanted glucose sensors.

Journal article J Biomed Mater Res A · December 1, 2008 Featured Publication The purpose of this study was to test the hypothesis that porous poly-L-lactic acid (PLLA) sensor coatings reduce fibrosis and promote blood microvessel formation in tissue adjacent to the sensor surface. Porous PLLA coatings were produced using ammonium b ... Full text Link to item Cite

IFATS collection: Adipose-derived stromal cells improve the foreign body response.

Journal article Stem Cells · October 2008 Featured Publication Many implanted devices fail due to the formation of an avascular capsule surrounding the device. Additionally, fat has long been known to promote healing and vascularization. The goals of this study were to identify potential mechanisms of the provascular ... Full text Link to item Cite

Advantages of minimally invasive, balloon-dissector-assisted tissue expander placement: a clinical and experimental study.

Journal article Ann Plast Surg · January 2008 Featured Publication BACKGROUND: Tissue expansion has been dependent upon healing the access incision after placement. Delay can be reduced with minimally placed expanders. Smaller, remote incisions allow for earlier expansion. METHODS: Balloon-assisted tissue expander placeme ... Full text Link to item Cite

Reduced foreign body response at nitric oxide-releasing subcutaneous implants.

Journal article Biomaterials · November 2007 Featured Publication The tissue response to nitric oxide (NO)-releasing subcutaneous implants is presented. Model implants were created by coating silicone elastomer with diazeniumdiolate-modified xerogel polymers capable of releasing NO. The host tissue response to such impla ... Full text Link to item Cite

Efficacy of ethyl nitrite in reversing surgical vasospasm.

Journal article J Reconstr Microsurg · July 2007 Featured Publication Vasospasm leading to thrombosis is a major cause of free flap failure. Flap blood flow may improve by application of vasodilating agents to the pedicle. The purpose of this study is to evaluate the effect of a novel S-nitrosylating agent, O-ethyl nitrite, ... Full text Link to item Cite

Vascular endothelial growth factor and dexamethasone release from nonfouling sensor coatings affect the foreign body response.

Journal article J Biomed Mater Res A · June 15, 2007 Featured Publication Vascular endothelial growth factor (VEGF) and dexamethasone (DX) release from hydrogel coatings were examined as a means to modify tissue inflammation and induce angiogenesis. Antibiofouling hydrogels for implantable glucose sensor coatings were prepared f ... Full text Link to item Cite

Combined bone allograft and adipose-derived stem cell autograft in a rabbit model.

Journal article Ann Plast Surg · May 2007 Featured Publication Currently available options for the repair of bony defects have substantial limitations. Much work has looked to the possibility of engineering bone using stem cells. These tissue-engineering efforts have focused on calvarial defect models, which have the ... Full text Link to item Cite

Adult adipose-derived stem cell attachment to biomaterials.

Journal article Biomaterials · February 2007 Featured Publication Attachment of adipose-derived stem cells (ASCs) to biomaterials prior to implantation is a possible strategy for mediating inflammation and wound healing. In this study, the ASC percent coverage was measured on common medical grade biosensor materials subj ... Full text Link to item Cite

Vein graft anastomoses with magnets.

Journal article J Plast Reconstr Aesthet Surg · 2007 Featured Publication BACKGROUND: Performing vascular anastomoses requires complete circumferential access and is time-consuming and technically challenging. The purpose of our study was to assess a sutureless magnetic device for anastomosing vein grafts. METHODS AND RESULTS: O ... Full text Link to item Cite

Direct demonstration of instabilities in oxygen concentrations within the extravascular compartment of an experimental tumor.

Journal article Cancer Res · February 15, 2006 Featured Publication To test the hypothesis that temporal variations in microvessel red cell flux cause unstable oxygen levels in tumor interstitium, extravascular oxygenation of R3230Ac mammary tumors grown in skin-fold window chambers was measured using recessed tip polarogr ... Full text Link to item Cite

The effects of ZD6474, an inhibitor of VEGF signaling, on cutaneous wound healing in mice.

Journal article J Surg Res · December 2005 Featured Publication BACKGROUND: ZD6474 is an inhibitor of the VEGFR-2 receptor tyrosine kinase with additional activity against EGFR-1 receptor tyrosine kinases that has been shown to inhibit tumor growth and wound-induced neovascularization in pre-clinical studies and phase ... Full text Link to item Cite

Inhibition of implant-associated infections via nitric oxide release.

Journal article Biomaterials · December 2005 Featured Publication The in vivo antibacterial activity of nitric oxide (NO)-releasing xerogel coatings was evaluated against an aggressive subcutaneous Staphylococcus aureus infection in a rat model. The NO-releasing implants were created by coating a medical-grade silicone e ... Full text Link to item Cite

In vivo performance of dual ligand augmented endothelialized expanded polytetrafluoroethylene vascular grafts.

Journal article J Biomed Mater Res B Appl Biomater · January 15, 2005 Featured Publication In this study, we examined combinations of three approaches to improve the adhesion of endothelial cells (EC) onto expanded polytetrafluoroethylene (ePTFE) vascular grafts placed at the femoral artery of rats: (1) high-affinity receptor-ligand binding of R ... Full text Link to item Cite

Method for improved accuracy in endogenous urea recovery marker calibrations for microdialysis in tumors.

Journal article J Pharmacol Toxicol Methods · 2005 Featured Publication INTRODUCTION: Urea has been proposed as an endogenous recovery marker for microdialysis for absolute concentration calculations of analytes in microdialysis samples. Previously we demonstrated a linear relationship between urea concentrations in a rat mamm ... Full text Link to item Cite

Engineered zinc finger-activating vascular endothelial growth factor transcription factor plasmid DNA induces therapeutic angiogenesis in rabbits with hindlimb ischemia.

Journal article Circulation · October 19, 2004 Featured Publication BACKGROUND: Therapeutic angiogenesis seeks to promote blood vessel growth to improve tissue perfusion. Vascular endothelial growth factor (VEGF) exists in multiple isoforms. We investigated an engineered zinc finger-containing transcription factor plasmid ... Full text Link to item Cite

Immunohistochemical identification of vascular endothelial growth factor in pig latissimus dorsi musculocutaneous flaps following ischemia-reperfusion injury.

Journal article Ann Plast Surg · October 2004 Featured Publication Vascular Endothelial Growth Factor (VEGF), a potent angiogenic, mitogenic and vascular permeability enhancing protein, appears to improve survival of ischemic flaps independent of its route of administration. The purpose of this study was to examine VEGF p ... Full text Link to item Cite

Side-to-side sutureless vascular anastomosis with magnets.

Journal article J Vasc Surg · September 2004 Featured Publication OBJECTIVE: Abbe and Payr introduced vascular techniques and devices to facilitate vessel anastomosis over a century ago. Obora published the idea of a sutureless vascular anastomosis with use of magnetic rings in 1978. The purpose of this study was to asse ... Full text Link to item Cite

Basic fibroblast growth factor expression following surgical delay of rat transverse rectus abdominis myocutaneous flaps.

Journal article Plast Reconstr Surg · June 2004 Featured Publication Partial transverse rectus abdominis myocutaneous (TRAM) flap loss in breast reconstruction can be a devastating complication for both patient and surgeon. Surgical delay of the TRAM flap has been shown to improve flap viability and has been advocated in "h ... Full text Link to item Cite

Vascular endothelial growth factor expression in pig latissimus dorsi myocutaneous flaps after ischemia reperfusion injury.

Journal article Plast Reconstr Surg · February 2003 Featured Publication Exogenous administration of vascular endothelial growth factor (VEGF) improves long-term viability of myocutaneous flaps. However, endogenous expression of this substance in flaps following ischemia-reperfusion injury has not been reported previously. Endo ... Full text Link to item Cite

Multimodality evaluation of pressure relief surfaces.

Journal article Plast Reconstr Surg · December 1998 Featured Publication Multimodality evaluation of six different support surfaces was performed measuring interface pressure, transcutaneous oxygen tension, and blood flow in pressure ulcer-prone areas on healthy subjects. Interface pressure was measured with a flexible force-se ... Full text Link to item Cite

Comparison of supine and lateral positioning on various clinically used support surfaces.

Journal article Ann Plast Surg · November 1998 Featured Publication In this study, interface pressures, transcutaneous oxygen tension (TcPO2), and blood flow were compared with the classic pressure ulcer-prone areas in the supine and lateral position on six different high- and low-air-loss support surfaces. Interface press ... Full text Link to item Cite

Pressure ulcers and pressure relief surfaces.

Journal article Clin Plast Surg · July 1998 Featured Publication Pressure ulcer incidence has been reduced but not eliminated. A few patients still develop pressure ulcers despite using air-fluidized beds and other specialty beds. In the future, very high-risk patients may possibly be sent to space clinics to recuperate ... Link to item Cite

Engineering the tissue which encapsulates subcutaneous implants. I. Diffusion properties.

Journal article J Biomed Mater Res · December 5, 1997 Featured Publication This report uses normal rat subcutis as a reference point to provide a quantitative analysis of small analyte transport through the tissue which encapsulates implants. Polyvinyl alcohol (PVA) with 60- and 350-micron mean pore size (PVA-60, PVA-350), nonpor ... Full text Link to item Cite

Heparin coating of vascular prostheses reduces thromboemboli.

Journal article Surgery · November 1997 Featured Publication BACKGROUND: Synthetic conduits made from currently available materials are suboptimal for use in small-diameter vascular reconstruction because of their high surface thrombogenicity, which leads to failure. METHODS: In this study control, heparin-irrigated ... Full text Link to item Cite

Modulation of ultraviolet light-induced epidermal damage: beneficial effects of tocopherol.

Journal article Plast Reconstr Surg · September 1997 Featured Publication Oxygen free radicals have been shown to result from and mediate deleterious effects of ultraviolet radiation on the skin. The purpose of this study was to determine if topical DL-alpha-tocopherol (vitamin E) could reduce ultraviolet-induced damage to the e ... Full text Link to item Cite

Iloprost in alginate decreases the thrombogenicity of expanded polytetrafluoroethylene.

Journal article J Reconstr Microsurg · May 1997 Featured Publication Vascular prostheses of small diameter perform poorly in vivo, in part because of the high thrombogenicity of available biomaterials. This study examined the thrombogenicity of expanded polytetrafluoroethylene (ePTFE) vascular graft segments (10 mm long x 4 ... Full text Link to item Cite

Rheology and the regulation of oxygen delivery.

Journal article Microsurgery · 1994 Featured Publication Full text Link to item Cite