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Carlos Perez Arques CV

Postdoctoral Associate
Molecular Genetics and Microbiology
Office hours 9:00 AM - 6:00 PM  
CV

Selected Publications


Heterochromatin and RNAi act independently to ensure genome stability in Mucorales human fungal pathogens.

Journal Article Proc Natl Acad Sci U S A · February 14, 2023 Chromatin modifications play a fundamental role in controlling transcription and genome stability and yet despite their importance, are poorly understood in early-diverging fungi. We present a comprehensive study of histone lysine and DNA methyltransferase ... Full text Link to item Cite

Transformation and CRISPR-Cas9-mediated homologous recombination in the fungus Rhizopus microsporus.

Journal Article STAR protocols · March 2022 Here, we describe a reliable approach for targeted DNA integrations in the genome of R. microsporus, one of the main causal agents of mucormycosis. We provide a strategy for stable, targeted integration of DNA templates by homologous recombination ( ... Full text Cite

Stable and reproducible homologous recombination enables CRISPR-based engineering in the fungus Rhizopus microsporus.

Journal Article Cell reports methods · December 2021 Mucormycosis is a lethal and emerging disease that has lacked a genetic model fulfilling both high virulence and the possibility of performing stable and reproducible gene manipulation by homologous recombination (HR). Here, we developed a new methodology ... Full text Cite

Role of the Non-Canonical RNAi Pathway in the Antifungal Resistance and Virulence of Mucorales.

Journal Article Genes · April 2021 Mucorales are the causal agents for the lethal disease known as mucormycosis. Mortality rates of mucormycosis can reach up to 90%, due to the mucoralean antifungal drug resistance and the lack of effective therapies. A concerning urgency among the medical ... Full text Cite

A Mucoralean White Collar-1 Photoreceptor Controls Virulence by Regulating an Intricate Gene Network during Host Interactions.

Journal Article Microorganisms · February 2021 Mucolares are an ancient group of fungi encompassing the causal agents for the lethal infection mucormycosis. The high lethality rates, the emerging character of this disease, and the broad antifungal resistance of its causal agents are mucormycosis featur ... Full text Cite

The RNAi Mechanism Regulates a New Exonuclease Gene Involved in the Virulence of Mucorales.

Journal Article International journal of molecular sciences · February 2021 Mucormycosis is a lethal disease caused by Mucorales, which are emerging as human causes that explain the high mortality for this disease. Consequently, the research community is searching for virulence determinants that could be repurposed as targets to d ... Full text Cite

The heterotrimeric G-protein beta subunit Gpb1 controls hyphal growth under low oxygen conditions through the protein kinase A pathway and is essential for virulence in the fungus Mucor circinelloides.

Journal Article Cellular microbiology · October 2020 Mucor circinelloides, a dimorphic opportunistic pathogen, expresses three heterotrimeric G-protein beta subunits (Gpb1, Gpb2 and Gpb3). The Gpb1-encoding gene is up-regulated during mycelial growth compared with that in the spore or yeast stage. gpb1 delet ... Full text Cite

A non-canonical RNAi pathway controls virulence and genome stability in Mucorales.

Journal Article PLoS genetics · July 2020 Epimutations in fungal pathogens are emerging as novel phenomena that could explain the fast-developing resistance to antifungal drugs and other stresses. These epimutations are generated by RNA interference (RNAi) mechanisms that transiently silence speci ... Full text Cite

Mucorales Species and Macrophages.

Journal Article Journal of fungi (Basel, Switzerland) · June 2020 Mucormycosis is an emerging fungal infection caused by Mucorales with an unacceptable high mortality rate. Mucorales is a complex fungal group, including eleven different genera that can infect humans. This heterogeneity is associated with species-specific ... Full text Cite

Genes, Pathways, and Mechanisms Involved in the Virulence of Mucorales.

Journal Article Genes · March 2020 The order Mucorales is a group of ancient fungi with limited tools for gene manipulation. The main consequence of this manipulation unwillingness is the limited knowledge about its biology compared to other fungal groups. However, the emerging of mucormyco ... Full text Cite

Comparative genomics applied to Mucor species with different lifestyles.

Journal Article BMC genomics · February 2020 BackgroundDespite a growing number of investigations on early diverging fungi, the corresponding lineages have not been as extensively characterized as Ascomycota or Basidiomycota ones. The Mucor genus, pertaining to one of these lineages is not a ... Full text Cite

Early Diverging Fungus Mucor circinelloides Lacks Centromeric Histone CENP-A and Displays a Mosaic of Point and Regional Centromeres.

Journal Article Curr Biol · November 18, 2019 Centromeres are rapidly evolving across eukaryotes, despite performing a conserved function to ensure high-fidelity chromosome segregation. CENP-A chromatin is a hallmark of a functional centromere in most organisms. Due to its critical role in kinetochore ... Full text Link to item Cite

Role of Arf-like proteins (Arl1 and Arl2) of Mucor circinelloides in virulence and antifungal susceptibility.

Journal Article Fungal genetics and biology : FG & B · August 2019 Mucor circinelloides is an etiologic agent of mucormycosis, a fungal infection produced by Mucorales often associated with mortality due to unavailability of antifungal drugs. Arl proteins belong to the Arf family and are involved in vesicle trafficking an ... Full text Cite

Mucor circinelloides Thrives inside the Phagosome through an Atf-Mediated Germination Pathway.

Journal Article mBio · February 5, 2019 Mucormycosis is an emerging fungal infection that is often lethal due to the ineffectiveness of current therapies. Here, we have studied the first stage of this infection-the germination of Mucor circinelloides spores inside phagocytic cells-from an integr ... Full text Link to item Cite

Heterotrimeric G-alpha subunits Gpa11 and Gpa12 define a transduction pathway that control spore size and virulence in Mucor circinelloides.

Journal Article PloS one · January 2019 Mucor circinelloides is one of the causal agents of mucormycosis, an emerging and high mortality rate fungal infection produced by asexual spores (sporangiospores) of fungi that belong to the order Mucorales. M. circinelloides has served as a model genetic ... Full text Cite

Understanding Mucor circinelloides pathogenesis by comparative genomics and phenotypical studies.

Journal Article Virulence · December 2018 The increasing number of infections by species of Mucorales and their high mortality constitute an important concern for public health. This study aims to decipher the genetic basis of Mucor circinelloides pathogenicity, which displays virulence in a strai ... Full text Cite

Control of morphology and virulence by ADP-ribosylation factors (Arf) in Mucor circinelloides.

Journal Article Current genetics · August 2018 Mucor circinelloides is a dimorphic fungus used to study cell differentiation that has emerged as a model to characterize mucormycosis. In this work, we identified four ADP-ribosylation factor (Arf)-encoding genes (arf1-arf4) and study their role in the mo ... Full text Cite

Components of a new gene family of ferroxidases involved in virulence are functionally specialized in fungal dimorphism.

Journal Article Scientific reports · May 2018 Mucormycosis is an emerging angio-invasive infection caused by Mucorales that presents unacceptable mortality rates. Iron uptake has been related to mucormycosis, since serum iron availability predisposes the host to suffer this infection. In addition, iro ... Full text Cite

Mucor circinelloides: Growth, Maintenance, and Genetic Manipulation.

Journal Article Current protocols in microbiology · May 2018 Mucor circinelloides is a fungus that belongs to the order Mucorales. It grows as mold in the environment and can cause mucormycosis, a potentially fatal infection in immunocompromised patients. M. circinelloides is a biodiesel producer and serves as a mod ... Full text Cite

Molecular Tools for Carotenogenesis Analysis in the Mucoral Mucor circinelloides.

Chapter · January 2018 The carotene producer Mucor circinelloides is the fungus within the Mucoromycota phylum with the widest repertoire of molecular tools to manipulate its genome. The initial development of an effective procedure for genetic transformation and later improveme ... Full text Cite

Heterochromatin and RNAi act independently to ensure genome stability in Mucorales human fungal pathogens.

Journal Article Proc Natl Acad Sci U S A · February 14, 2023 Chromatin modifications play a fundamental role in controlling transcription and genome stability and yet despite their importance, are poorly understood in early-diverging fungi. We present a comprehensive study of histone lysine and DNA methyltransferase ... Full text Link to item Cite

Transformation and CRISPR-Cas9-mediated homologous recombination in the fungus Rhizopus microsporus.

Journal Article STAR protocols · March 2022 Here, we describe a reliable approach for targeted DNA integrations in the genome of R. microsporus, one of the main causal agents of mucormycosis. We provide a strategy for stable, targeted integration of DNA templates by homologous recombination ( ... Full text Cite

Stable and reproducible homologous recombination enables CRISPR-based engineering in the fungus Rhizopus microsporus.

Journal Article Cell reports methods · December 2021 Mucormycosis is a lethal and emerging disease that has lacked a genetic model fulfilling both high virulence and the possibility of performing stable and reproducible gene manipulation by homologous recombination (HR). Here, we developed a new methodology ... Full text Cite

Role of the Non-Canonical RNAi Pathway in the Antifungal Resistance and Virulence of Mucorales.

Journal Article Genes · April 2021 Mucorales are the causal agents for the lethal disease known as mucormycosis. Mortality rates of mucormycosis can reach up to 90%, due to the mucoralean antifungal drug resistance and the lack of effective therapies. A concerning urgency among the medical ... Full text Cite

A Mucoralean White Collar-1 Photoreceptor Controls Virulence by Regulating an Intricate Gene Network during Host Interactions.

Journal Article Microorganisms · February 2021 Mucolares are an ancient group of fungi encompassing the causal agents for the lethal infection mucormycosis. The high lethality rates, the emerging character of this disease, and the broad antifungal resistance of its causal agents are mucormycosis featur ... Full text Cite

The RNAi Mechanism Regulates a New Exonuclease Gene Involved in the Virulence of Mucorales.

Journal Article International journal of molecular sciences · February 2021 Mucormycosis is a lethal disease caused by Mucorales, which are emerging as human causes that explain the high mortality for this disease. Consequently, the research community is searching for virulence determinants that could be repurposed as targets to d ... Full text Cite

The heterotrimeric G-protein beta subunit Gpb1 controls hyphal growth under low oxygen conditions through the protein kinase A pathway and is essential for virulence in the fungus Mucor circinelloides.

Journal Article Cellular microbiology · October 2020 Mucor circinelloides, a dimorphic opportunistic pathogen, expresses three heterotrimeric G-protein beta subunits (Gpb1, Gpb2 and Gpb3). The Gpb1-encoding gene is up-regulated during mycelial growth compared with that in the spore or yeast stage. gpb1 delet ... Full text Cite

A non-canonical RNAi pathway controls virulence and genome stability in Mucorales.

Journal Article PLoS genetics · July 2020 Epimutations in fungal pathogens are emerging as novel phenomena that could explain the fast-developing resistance to antifungal drugs and other stresses. These epimutations are generated by RNA interference (RNAi) mechanisms that transiently silence speci ... Full text Cite

Mucorales Species and Macrophages.

Journal Article Journal of fungi (Basel, Switzerland) · June 2020 Mucormycosis is an emerging fungal infection caused by Mucorales with an unacceptable high mortality rate. Mucorales is a complex fungal group, including eleven different genera that can infect humans. This heterogeneity is associated with species-specific ... Full text Cite

Genes, Pathways, and Mechanisms Involved in the Virulence of Mucorales.

Journal Article Genes · March 2020 The order Mucorales is a group of ancient fungi with limited tools for gene manipulation. The main consequence of this manipulation unwillingness is the limited knowledge about its biology compared to other fungal groups. However, the emerging of mucormyco ... Full text Cite

Comparative genomics applied to Mucor species with different lifestyles.

Journal Article BMC genomics · February 2020 BackgroundDespite a growing number of investigations on early diverging fungi, the corresponding lineages have not been as extensively characterized as Ascomycota or Basidiomycota ones. The Mucor genus, pertaining to one of these lineages is not a ... Full text Cite

Early Diverging Fungus Mucor circinelloides Lacks Centromeric Histone CENP-A and Displays a Mosaic of Point and Regional Centromeres.

Journal Article Curr Biol · November 18, 2019 Centromeres are rapidly evolving across eukaryotes, despite performing a conserved function to ensure high-fidelity chromosome segregation. CENP-A chromatin is a hallmark of a functional centromere in most organisms. Due to its critical role in kinetochore ... Full text Link to item Cite

Role of Arf-like proteins (Arl1 and Arl2) of Mucor circinelloides in virulence and antifungal susceptibility.

Journal Article Fungal genetics and biology : FG & B · August 2019 Mucor circinelloides is an etiologic agent of mucormycosis, a fungal infection produced by Mucorales often associated with mortality due to unavailability of antifungal drugs. Arl proteins belong to the Arf family and are involved in vesicle trafficking an ... Full text Cite

Mucor circinelloides Thrives inside the Phagosome through an Atf-Mediated Germination Pathway.

Journal Article mBio · February 5, 2019 Mucormycosis is an emerging fungal infection that is often lethal due to the ineffectiveness of current therapies. Here, we have studied the first stage of this infection-the germination of Mucor circinelloides spores inside phagocytic cells-from an integr ... Full text Link to item Cite

Heterotrimeric G-alpha subunits Gpa11 and Gpa12 define a transduction pathway that control spore size and virulence in Mucor circinelloides.

Journal Article PloS one · January 2019 Mucor circinelloides is one of the causal agents of mucormycosis, an emerging and high mortality rate fungal infection produced by asexual spores (sporangiospores) of fungi that belong to the order Mucorales. M. circinelloides has served as a model genetic ... Full text Cite

Understanding Mucor circinelloides pathogenesis by comparative genomics and phenotypical studies.

Journal Article Virulence · December 2018 The increasing number of infections by species of Mucorales and their high mortality constitute an important concern for public health. This study aims to decipher the genetic basis of Mucor circinelloides pathogenicity, which displays virulence in a strai ... Full text Cite

Control of morphology and virulence by ADP-ribosylation factors (Arf) in Mucor circinelloides.

Journal Article Current genetics · August 2018 Mucor circinelloides is a dimorphic fungus used to study cell differentiation that has emerged as a model to characterize mucormycosis. In this work, we identified four ADP-ribosylation factor (Arf)-encoding genes (arf1-arf4) and study their role in the mo ... Full text Cite

Components of a new gene family of ferroxidases involved in virulence are functionally specialized in fungal dimorphism.

Journal Article Scientific reports · May 2018 Mucormycosis is an emerging angio-invasive infection caused by Mucorales that presents unacceptable mortality rates. Iron uptake has been related to mucormycosis, since serum iron availability predisposes the host to suffer this infection. In addition, iro ... Full text Cite

Mucor circinelloides: Growth, Maintenance, and Genetic Manipulation.

Journal Article Current protocols in microbiology · May 2018 Mucor circinelloides is a fungus that belongs to the order Mucorales. It grows as mold in the environment and can cause mucormycosis, a potentially fatal infection in immunocompromised patients. M. circinelloides is a biodiesel producer and serves as a mod ... Full text Cite

Molecular Tools for Carotenogenesis Analysis in the Mucoral Mucor circinelloides.

Chapter · January 2018 The carotene producer Mucor circinelloides is the fungus within the Mucoromycota phylum with the widest repertoire of molecular tools to manipulate its genome. The initial development of an effective procedure for genetic transformation and later improveme ... Full text Cite

RNAi-Based Functional Genomics Identifies New Virulence Determinants in Mucormycosis.

Journal Article PLoS pathogens · January 2017 Mucorales are an emerging group of human pathogens that are responsible for the lethal disease mucormycosis. Unfortunately, functional studies on the genetic factors behind the virulence of these organisms are hampered by their limited genetic tractability ... Full text Cite