Scholars@Duke
Jun Chen

Jun Chen

Professor of Medicine
Medicine, Hematologic Malignancies and Cellular Therapy
chen0032@mc.duke.edu
Box 103866 Med Ctr, Durham, NC 27710
GSRB1 Rm 4004B, 905 S LaSalle Street, Durham, NC 27710

Scholarly Works

Imatinib-Sensitive Chronic Myeloid Leukaemia Is Associated with mtDNA Mutations and Reduced Oxphos Capacity

Conference Blood · November 5, 2024 Introduction: Therapy resistance driven by Leukemic Stem/Progenitor Cells (LSPCs) remains a challenge in Chronic Myeloid Leukemia (CML).Recent studies indicate that Tyrosine Kinase Inhibi ... Full text Cite

NT-I7, a Long-Acting Interleukin-7 Molecule, Promotes T Cell Reconstitution Following Radiation Injury through Thymic-Dependent and Independent Pathways

Conference Blood · November 5, 2024 Total body irradiation (TBI) causes suppression of hematopoiesis and T cell depletion. T cell lymphopenia following radiation exposure increases the risk of opportunistic infections. Curr ... Full text Cite

Human Endothelial Cell-Derived Extracellular Vesicles Mitigate Radiation-Induced Hematopoietic Injury

Conference Blood · November 5, 2024 Endothelial cells (ECs) are the major participants in bone marrow microenvironment and key regulators of hematopoiesis. ECs regulate hematopoiesis at least in part by shedding the extrace ... Full text Cite

Endothelial Cell Derived Extracellular Vesicles and Hematopoiesis.

Journal article Radiat Res · August 1, 2024 Extracellular vesicles (EVs) have been recognized as a novel way of cell-to-cell communication in the last several decades. It is believed that EVs exert their functions on nearby or distant cells through transfer of the cargo that they carry. In this revi ... Full text Link to item Cite

Exogenous Growth Hormone Exacerbates Post-Irradiation Atherosclerosis in Susceptible Epicardial Coronary Arteries.

Journal article Toxicol Pathol · August 2024 Cardiac exposure to ionizing radiation can damage both the microvasculature and coronary arteries, as well as increase the long-term risk of heart disease, myocardial fibrosis, and conduction abnormalities. Therapeutic agents capable of promoting recovery ... Full text Link to item Cite

Prebiotic galactooligosaccharides interact with mouse gut microbiota to attenuate acute graft-versus-host disease.

Journal article Blood · November 24, 2022 Previous studies suggest that gut microbiome disruption induced by chemotherapy, dietary deficiencies, and/or antibiotics are associated with increased incidence of acute graft-versus-host disease (aGVHD) following hematopoietic stem cell transplantation ( ... Full text Link to item Cite

Chronic GVHD of the CNS.

Journal article Blood · March 3, 2022 Full text Link to item Cite

Targeting Glycolysis in Alloreactive T Cells to Prevent Acute Graft-Versus-Host Disease While Preserving Graft-Versus-Leukemia Effect.

Journal article Front Immunol · 2022 Alloreactive donor T cells undergo extensive metabolic reprogramming to become activated and induce graft-versus-host disease (GVHD) upon alloantigen encounter. It is generally thought that glycolysis, which promotes T cell growth and clonal expansion, is ... Full text Link to item Cite

Alphavirus Replicon Particle Vaccine Breaks B Cell Tolerance and Rapidly Induces IgG to Murine Hematolymphoid Tumor Associated Antigens.

Journal article Front Immunol · 2022 De novo immune responses to myeloid and other blood-borne tumors are notably limited and ineffective, making our ability to promote immune responses with vaccines a major challenge. While focus has been largely on cytotoxic cell-mediated tumor eradication, ... Full text Open Access Link to item Cite

Bob.1 Blocks a SYK Protein Degradation Pathway in Mice That Develop Chronic Graft Versus Host Disease Manifestations

Conference Blood · November 5, 2021 AbstractAfter elucidating a role for aberrant B Cell Receptor (BCR) signaling in chronic graft versus host disease (cGVHD) (Allen et al, Blood. 2014;123:2108), we showed that the proximal BCR molecule SYK is ... Full text Cite

Proteomics and Transcriptomics of the Hippocampus and Cortex in SUDEP and High-Risk SUDEP Patients.

Journal article Neurology · May 25, 2021 OBJECTIVE: To identify the molecular signaling pathways underlying sudden unexpected death in epilepsy (SUDEP) and high-risk SUDEP compared to control patients with epilepsy. METHODS: For proteomics analyses, we evaluated the hippocampus and frontal cortex ... Full text Link to item Cite

BAFF promotes heightened BCR responsiveness and manifestations of chronic GVHD after allogeneic stem cell transplantation.

Journal article Blood · May 6, 2021 Patients with chronic graft-versus-host disease (cGVHD) have increased B cell-activating factor (BAFF) levels, but whether BAFF promotes disease after allogeneic bone marrow transplantation (allo-BMT) remains unknown. In a major histocompatibility complex- ... Full text Link to item Cite

Phase I dose escalation study of naive T-cell depleted donor lymphocyte infusion following allogeneic stem cell transplantation.

Journal article Bone Marrow Transplant · January 2021 Prophylactic donor lymphocyte infusions (DLI) are used to augment post-transplant immune recovery to reduce both infectious complications and disease recurrence. Preclinical studies implicate the naive T-cell subset as the primary driver of graft-versus-ho ... Full text Link to item Cite

Analysis of the Circular Transcriptome in the Synaptosomes of Aged Mice.

Journal article Neuroscience · November 21, 2020 Recently, circular RNAs (circRNAs) have been revealed to be an important non-coding element of the transcriptome. The brain contains the most abundant and widespread expression of circRNA. There are also indications that the circular transcriptome undergoe ... Full text Link to item Cite

Fibrinogen-Coated Albumin Nanospheres Prevent Thrombocytopenia-Related Bleeding.

Journal article Radiat Res · August 1, 2020 Thrombocytopenia (TCP) may cause severe and life-threatening bleeding. While this may be prevented by platelet transfusions, transfusions are associated with potential complications, do not always work (platelet refractory) and are not always available. Th ... Full text Link to item Cite

Coding and non-coding transcriptome of mesial temporal lobe epilepsy: Critical role of small non-coding RNAs.

Journal article Neurobiol Dis · February 2020 Our understanding of mesial temporal lobe epilepsy (MTLE), one of the most common form of drug-resistant epilepsy in humans, is derived mainly from clinical, imaging, and physiological data from humans and animal models. High-throughput gene expression stu ... Full text Link to item Cite

T cell metabolism in graft-versus-host disease.

Journal article Blood Sci · January 2020 Graft-versus-host disease (GVHD) is a major source of morbidity and mortality following allogeneic hematopoietic stem cell transplant (allo-HSCT), one of the most effective approaches to treat hematopoietic malignancies.1 However, current prophylaxis regim ... Full text Link to item Cite

Early transcriptome changes in response to chemical long-term potentiation induced via activation of synaptic NMDA receptors in mouse hippocampal neurons.

Journal article Genomics · December 2019 Long term potentiation (LTP) is a form of synaptic plasticity. In the present study LTP was induced via activation of synaptic NMDA receptors in primary hippocampal neuron cultures from neonate mice and RNA was isolated for RNA sequencing at 20 min followi ... Full text Link to item Cite

Microrna-191 Regulates T-Cell Clonal Expansion during Graft-Versus-Host Disease

Conference Blood · November 13, 2019 Allogeneic hematopoietic cell transplantation is a potentially curative treatment choice for a wide variety of hematological malignancies. However, graft-versus-host disease (GVHD), which is mediated by donor alloreactive T cells, limits the succes ... Full text Cite

Tracing Tumor Evolution in Sarcoma Reveals Clonal Origin of Advanced Metastasis.

Journal article Cell Rep · September 10, 2019 Cellular heterogeneity is frequently observed in cancer, but the biological significance of heterogeneous tumor clones is not well defined. Using multicolor reporters and CRISPR-Cas9 barcoding, we trace clonal dynamics in a mouse model of sarcoma. We show ... Full text Link to item Cite

Changes in circular RNA expression patterns during human foetal brain development.

Journal article Genomics · July 2019 Circular RNAs (circRNAs) are a recently identified class of long non-coding RNAs and their expression is regulated in a tissue- and developmental stage-specific manner. Recent studies indicate the potential regulatory role that circRNAs may have, particula ... Full text Link to item Cite

Endothelial Cell-Derived Extracellular Vesicles Mitigate Radiation-Induced Hematopoietic Injury.

Journal article Int J Radiat Oncol Biol Phys · June 1, 2019 PURPOSE: Extracellular vesicles (EVs) are shed vesicles that bear a combination of nucleic acids and proteins. EVs are becoming recognized as a mode of cell-to-cell communication. Because hematopoietic stem cells reside in proximity to endothelial cells (E ... Full text Link to item Cite

Donor Allospecific CD44high Central Memory T Cells Have Decreased Ability to Mediate Graft-vs.-Host Disease.

Journal article Front Immunol · 2019 Data from both animal models and humans have demonstrated that effector memory T cells (TEM) and central memory T cells (TCM) from unprimed donors have decreased ability to induce graft-vs-host disease (GVHD). Allospecific TEM from primed donors do not med ... Full text Link to item Cite

Biodistribution and sensitive tracking of immune cells with plasmonic gold nanostars.

Journal article Int J Nanomedicine · 2019 Aim: To quantitatively and sensitively investigate the biodistribution of immune cells after systemic administration. Methods: Immune cells were loaded with plasmonic gold nanostars (GNS) tracking probes. Inductively coupled plasma mass spectrometry (ICP-M ... Full text Link to item Cite

Endothelial Cell-Derived Extracellular Vesicles Mitigate Radiation-Induced Hematopoietic Injury

Conference Blood · November 29, 2018 AbstractHematopoietic stem cells (HSCs) reside in specialized microenvironments and adjacent to endothelial cells (ECs), from which they receive regulatory cues. Following injury from radiation or chemothera ... Full text Cite

SYK inhibitor entospletinib prevents ocular and skin GVHD in mice.

Journal article JCI Insight · October 4, 2018 Graft-versus-host disease (GVHD) is a major complication of hematopoietic stem cell transplantation (HCT). The tyrosine kinase SYK contributes to both acute and chronic GVHD development, making it an attractive target for GVHD prevention. Entospletinib (EN ... Full text Link to item Cite

Multiple System Atrophy: Many Lessons from the Transcriptome.

Journal article Neuroscientist · June 2018 Multiple system atrophy (MSA) is a complex, multifactorial, debilitating neurodegenerative disease that is often misdiagnosed and misunderstood. MSA has two subclasses, MSA-P and MSA-C, defined by the dominance of parkinsonism or cerebellar dysfunction in ... Full text Link to item Cite

Tropomyosin isoforms have specific effects on the transcriptome of undifferentiated and differentiated B35 neuroblastoma cells.

Journal article FEBS Open Bio · April 2018 UNLABELLED: Tropomyosins, a family of actin-associated proteins, bestow actin filaments with distinct biochemical and physical properties which are important for determining cell shape and regulating many cellular processes in eukaryotic cells. Here, we us ... Full text Link to item Cite

Region-specific expression of circular RNAs in the mouse brain.

Journal article Neurosci Lett · February 14, 2018 Circular RNAs (circRNAs) are abundant in mammalian brain and their expression is regulated in a tissue- and developmental stage-specific manner. Mammalian brain is the most transcriptionally complex organ. While many studies have extensively studied linear ... Full text Link to item Cite

Epidermal Growth Factor and Granulocyte Colony Stimulating Factor Signaling Are Synergistic for Hematopoietic Regeneration.

Journal article Stem Cells · February 2018 Hematopoietic regeneration following chemotherapy may be distinct from regeneration following radiation. While we have shown that epidermal growth factor (EGF) accelerates regeneration following radiation, its role following chemotherapy is currently unkno ... Full text Link to item Cite

Transcriptome landscape of long intergenic non-coding RNAs in endometrial cancer.

Journal article Gynecol Oncol · December 2017 UNLABELLED: Endometrial cancer is the most common gynecological malignancy in the developed world. It is the fifth most common cancer and accounts for 4.8% of all cancers in women. Long intergenic non-coding RNAs (lincRNAs), a subclass of long non-coding R ... Full text Link to item Cite

Thioredoxin mitigates radiation-induced hematopoietic stem cell injury in mice.

Journal article Stem cell research & therapy · November 2017 BackgroundRadiation exposure poses a significant threat to public health. Hematopoietic injury is one of the major manifestations of acute radiation sickness. Protection and/or mitigation of hematopoietic stem cells (HSCs) from radiation injury is ... Full text Cite

The emerging role of circular RNAs in transcriptome regulation.

Journal article Genomics · October 2017 Circular RNAs (circRNAs) are a recently discovered form of RNA that has been found to regulate mammalian transcription. CircRNAs are covalently closed, single-stranded transcripts produced from precursor mRNA. While initially circRNAs were considered to be ... Full text Link to item Cite

RNA sequencing reveals pronounced changes in the noncoding transcriptome of aging synaptosomes.

Journal article Neurobiol Aging · August 2017 Normal aging is associated with impairments in cognitive functions. These alterations are caused by diminutive changes in the biology of synapses, and ineffective neurotransmission, rather than loss of neurons. Hitherto, only a few studies, exploring molec ... Full text Link to item Cite

Thymus Transplantation for Chronic Graft Versus Host Disease

Conference Journal of Allergy and Clinical Immunology · February 2017 Full text Cite

Recipient-Derived BAFF and Alloantigen Synergistically Activate B Cells in Murine Chronic Gvhd

Conference Blood · December 2, 2016 AbstractIncreased B cell-activating factor (BAFF) and aberrant B cell survival and activation are associated with chronic graft versus host disease (cGVHD) in patients. Whether excessive BAFF production has ... Full text Cite

EGF Accelerates Hematopoietic Stem Cell Regeneration Following 5-FU Chemotherapy Via G-CSF Receptor Signaling

Conference Blood · December 2, 2016 AbstractHematopoietic stem cells (HSCs) reside in specialized bone marrow microenvironments adjacent to endothelial cells (BM ECs). BM ECs regulate both HSC self-renewal and regeneration by expressing solubl ... Full text Cite

All-Trans Retinoic Acid (ATRA) Targets IRF4 Deficient, NOTCH2-Activated B Cells from Chronic Gvhd Patients

Conference Blood · December 2, 2016 AbstractAllogeneic hematopoietic stem cell transplantation (HCT)-related immune pathology severely limits patient (Pt) survival, largely due to infections. Chronic graft versus host disease (cGVHD) Pts are p ... Full text Cite

Characterization of circular RNAs landscape in multiple system atrophy brain.

Journal article J Neurochem · November 2016 Circular RNAs (circRNAs) have been recently identified as a naturally occurring family of widespread and diverse endogenous non-coding RNAs that may regulate gene expression in mammals. They are unusually stable RNA molecules with cell type- or development ... Full text Link to item Cite

The emerging role of long non-coding RNAs in endometrial cancer.

Journal article Cancer Genet · October 2016 The human genome is pervasively transcribed and approximately 98% of the genome is non-coding. Long non-coding RNAs (lncRNAs) are a heterogeneous group of RNA transcripts that are >200 nucleotides in length with minimal to no protein-coding potential. Simi ... Full text Link to item Cite

Uncommon structural motifs dominate the antigen binding site in human autoantibodies reactive with basement membrane collagen.

Journal article Mol Immunol · August 2016 Autoantibodies mediate organ destruction in multiple autoimmune diseases, yet their origins in patients remain poorly understood. To probe the genetic origins and structure of disease-associated autoantibodies, we engrafted immunodeficient mice with human ... Full text Link to item Cite

LINC00507 Is Specifically Expressed in the Primate Cortex and Has Age-Dependent Expression Patterns.

Journal article J Mol Neurosci · August 2016 Over the past decade, there has been an increase in the appreciation of the role of non-coding RNA in the development of organism phenotype. It is possible to divide the non-coding elements of the transcriptome into three categories: short non-coding RNAs, ... Full text Link to item Cite

RNA-Sequencing to Elucidate Early Patterns of Dysregulation Underlying the Onset of Alzheimer's Disease.

Book section · 2016 With its ability to perform rapid transcriptome profiling and profound transcriptomic analysis powered by high-throughput sequencing at a high resolution with deep coverage, the advent of RNA sequencing technology, RNA-Seq, outperforms other methods in the ... Full text Link to item Cite

The Antisense Transcriptome and the Human Brain.

Journal article J Mol Neurosci · January 2016 The transcriptome of a cell is made up of a varied array of RNA species, including protein-coding RNAs, long non-coding RNAs, short non-coding RNAs, and circular RNAs. The cellular transcriptome is dynamic and can change depending on environmental factors, ... Full text Link to item Cite

Differential Requirements of TCR Signaling in Homeostatic Maintenance and Function of Dendritic Epidermal T Cells.

Journal article J Immunol · November 1, 2015 Dendritic epidermal T cells (DETCs) are generated exclusively in the fetal thymus and maintained in the skin epithelium throughout postnatal life of the mouse. DETCs have restricted antigenic specificity as a result of their exclusive usage of a canonical ... Full text Link to item Cite

Recovery of a human natural antibody against the noncollagenous-1 domain of type IV collagen using humanized models.

Journal article J Transl Med · June 6, 2015 BACKGROUND: Anti-glomerular basement membrane nephritis and Goodpasture syndrome result from autoantibody (Ab)-mediated destruction of kidney and lung. Ab target the noncollagenous 1 (NC1) domain of alpha3(IV) collagen, but little is known about Ab origins ... Full text Link to item Cite

Fibrinogen-Coated Nanospheres Prevent Thrombocytopenia-Related Bleeding

Conference Biology of Blood and Marrow Transplantation · February 2015 Full text Cite

High expression of long intervening non-coding RNA OLMALINC in the human cortical white matter is associated with regulation of oligodendrocyte maturation.

Journal article Mol Brain · January 10, 2015 BACKGROUND: Long intervening non-coding RNAs (lincRNAs) are a recently discovered subclass of non-coding RNAs. LincRNAs are expressed across the mammalian genome and contribute to the pervasive transcription phenomenon. They display a tissue-specific and s ... Full text Link to item Cite

The glucose transporter Glut1 is selectively essential for CD4 T cell activation and effector function.

Journal article Cell Metab · July 1, 2014 CD4 T cell activation leads to proliferation and differentiation into effector (Teff) or regulatory (Treg) cells that mediate or control immunity. While each subset prefers distinct glycolytic or oxidative metabolic programs in vitro, requirements and mech ... Full text Link to item Cite

Toward an organ based dose prescription method for the improved accuracy of murine dose in orthovoltage x-ray irradiators.

Journal article Med Phys · March 2014 PURPOSE: Accurate dosimetry is essential when irradiating mice to ensure that functional and molecular endpoints are well understood for the radiation dose delivered. Conventional methods of prescribing dose in mice involve the use of a single dose rate me ... Full text Link to item Cite

Central Memory T Cells That Express High Level Of CD44 Mediate Strong Anti-Tumor Response Without Gvhd

Conference Blood · November 15, 2013 AbstractSeveral groups have demonstrated directly and indirectly that central memory T cells (TCM) have decreased ability to induce graft-versus-host disease (GVHD) compared with naïve T cells (TN). We have ... Full text Cite

Insulin-like growth factor 1 mitigates hematopoietic toxicity after lethal total body irradiation.

Journal article Int J Radiat Oncol Biol Phys · March 15, 2013 PURPOSE: To investigate whether and how insulin-like growth factor 1 (IGF-1) mitigates hematopoietic toxicity after total body irradiation. METHODS AND MATERIALS: BALB/c mice were irradiated with a lethal dose of radiation (7.5 Gy) and treated with IGF-1 a ... Full text Link to item Cite

Insulin-Like Growth Factor 1 Mitigates Hematopoietic Toxicity After Lethal Total Body Irradiation

Conference Biology of Blood and Marrow Transplantation · February 2013 Full text Cite

Unique transcriptome patterns of the white and grey matter corroborate structural and functional heterogeneity in the human frontal lobe.

Journal article PLoS One · 2013 The human frontal lobe has undergone accelerated evolution, leading to the development of unique human features such as language and self-reflection. Cortical grey matter and underlying white matter reflect distinct cellular compositions in the frontal lob ... Full text Link to item Cite

Long-term in vivo imaging of multiple organs at the single cell level.

Journal article PLoS One · 2013 Two-photon microscopy has enabled the study of individual cell behavior in live animals. Many organs and tissues cannot be studied, especially longitudinally, because they are located too deep, behind bony structures or too close to the lung and heart. Her ... Full text Link to item Cite

Allospecific CD4(+) effector memory T cells do not induce graft-versus-host disease in mice.

Journal article Biol Blood Marrow Transplant · October 2012 We studied whether allospecific CD4(+) effector memory T cells (T(EM)) could induce graft-versus-host disease (GVHD) using a novel GVHD model induced solely by CD4(+) T cell receptor transgenic TEa cells. Allospecific T(EM) generated in a lymphopenic host ... Full text Link to item Cite

CD62L- memory T cells enhance T-cell regeneration after allogeneic stem cell transplantation by eliminating host resistance in mice.

Journal article Blood · June 28, 2012 A major challenge in allogeneic hematopoietic cell transplantation is how to transfer T-cell immunity without causing graft-versus-host disease (GVHD). Effector memory T cells (CD62L(-)) are a cell subset that can potentially address this challenge because ... Full text Link to item Cite

An ear punch model for studying the effect of radiation on wound healing.

Journal article Int J Radiat Biol · August 2011 PURPOSE: Radiation and wound combined injury represents a major clinical challenge because of the synergistic interactions that lead to higher morbidity and mortality than either insult would produce singly. The purpose of this study was to develop a mouse ... Full text Link to item Cite

Prevention of GVHD without losing GVL effect: windows of opportunity.

Journal article Immunol Res · April 2011 Allogeneic hematopoietic cell transplantation has developed into a most successful form of immunotherapy for hematologic malignancies in the past 50 years. However, its effectiveness and wider applications have been greatly limited by the development of gr ... Full text Link to item Cite

Progenitor cell dose determines the pace and completeness of engraftment in a xenograft model for cord blood transplantation.

Journal article Blood · December 16, 2010 Two critical concerns in clinical cord blood transplantation are the initial time to engraftment and the subsequent restoration of immune function. These studies measured the impact of progenitor cell dose on both the pace and strength of hematopoietic rec ... Full text Link to item Cite

Selective enhancement of donor hematopoietic cell engraftment by the CXCR4 antagonist AMD3100 in a mouse transplantation model.

Journal article PLoS One · June 28, 2010 The interaction between stromal cell-derived factor-1 (SDF-1) with CXCR4 chemokine receptors plays an important role in hematopoiesis following hematopoietic stem cell transplantation. We examined the efficacy of post transplant administration of a specifi ... Full text Open Access Link to item Cite

Growth hormone mitigates against lethal irradiation and enhances hematologic and immune recovery in mice and nonhuman primates.

Journal article PLoS One · June 16, 2010 Medications that can mitigate against radiation injury are limited. In this study, we investigated the ability of recombinant human growth hormone (rhGH) to mitigate against radiation injury in mice and nonhuman primates. BALB/c mice were irradiated with 7 ... Full text Open Access Link to item Cite

Novel mechanism of rapamycin in GVHD: increase in interstitial regulatory T cells.

Journal article Bone Marrow Transplant · February 2010 Rapamycin (RAPA) is an immunosuppressive drug that prevents and treats graft-versus-host disease (GVHD) after allogeneic hematopoietic cell transplant (HCT). One possible mechanism for its efficacy is induction of tolerance, through increased number or enh ... Full text Link to item Cite

Increased skin carcinogenesis in caspase-activated DNase knockout mice.

Journal article Carcinogenesis · October 2009 Caspase-activated DNase (CAD), also called DNA fragmentation factor (DFF), is the enzyme responsible for DNA fragmentation during apoptosis, a hallmark of programmed cell death. CAD/DFF has been shown to suppress radiation-induced carcinogenesis by prevent ... Full text Link to item Cite

Glomerular type 1 angiotensin receptors augment kidney injury and inflammation in murine autoimmune nephritis.

Journal article J Clin Invest · April 2009 Studies in humans and animal models indicate a key contribution of angiotensin II to the pathogenesis of glomerular diseases. To examine the role of type 1 angiotensin (AT1) receptors in glomerular inflammation associated with autoimmune disease, we genera ... Full text Link to item Cite

Endothelial progenitor cell infusion induces hematopoietic stem cell reconstitution in vivo.

Journal article Blood · February 26, 2009 Hematopoietic stem cells (HSCs) reside in association with bone marrow (BM) sinusoidal vessels in vivo, but the function of BM endothelial cells (ECs) in regulating hematopoiesis is unclear. We hypothesized that hematopoietic regeneration following injury ... Full text Link to item Cite

Human Memory T CELL S Elicit Decreased Cytotoxicity Against Allogeneic Targets

Conference Biology of Blood and Marrow Transplantation · February 2009 Full text Cite

Complete Long-Term Hematopoietic Engraftment by Lineage-Depleted ALDH CD34+ UCB Cells

Conference Biology of Blood and Marrow Transplantation · February 2009 Full text Cite

366: Allospecific Effector Memory T cells have Decreased Ability to Induce Acute GVHD

Conference Biology of Blood and Marrow Transplantation · February 2008 Full text Cite

Allogeneic memory T cell response

Journal article BIOLOGY OF BLOOD AND MARROW TRANSPLANTATION · January 1, 2008 Link to item Cite

Allogeneic memory T cell response.

Journal article Biol Blood Marrow Transplant · January 2008 Recent data have revealed marked differences in alloresponses mediated by memory T cells during GVH and host-versus-graft (HVG) reactions (summarized in Table 1). Even though the mechanisms are not completely understood, it is clear that all memory T cells ... Link to item Cite

Prevention of Graft-Versus-Host Disease in Mouse Model Using Anti-Mouse C5 Antibody.

Conference Blood · November 16, 2007 AbstractGraft versus-host disease (GVHD) is a major cause of morbidity and mortality after bone marrow transplantation (BMT). An allogeneic GVH reaction is a response of donor lymphoid cells to host minor or ... Full text Cite

Treatment of Established Graft-Versus-Host Disease by PG490-88.

Conference Blood · November 16, 2007 AbstractGraft-versus-host disease (GVHD) is a major complication following hematopoietic cell transplantation. While prophylaxis of GVHD has improved, treatment for GVHD remains unsatisfactory. PG490–88, a s ... Full text Cite

Allogeneic Committed Hematopoietic Progenitors Are Protective Against Radiation.

Conference Blood · November 16, 2007 AbstractWhole-body irradiation may lead to bone marrow failure and death. It was previously reported that congenic myeloerythroid-restricted progenitors are able to radioprotect lethally irradiated animals. ... Full text Cite

Alloimmune lung injury induced by local innate immune activation through inhaled lipopolysaccharide.

Journal article Transplantation · October 27, 2007 BACKGROUND: Alloimmune lung injury, characterized by perivascular lymphocytic inflammation, lymphocytic bronchiolitis (LB), and obliterative bronchiolitis (OB), causes substantial morbidity and mortality after lung transplantation and bone marrow transplan ... Full text Link to item Cite

High-resolution in vivo imaging of blood vessels without labeling.

Journal article Opt Lett · September 15, 2007 We demonstrate that both oxyhemoglobin and deoxyhemoglobin have sequential two-color, two-photon absorption properties that can serve as endogenous contrasts in microvasculature imaging. Using a sensitive modulation transfer technique, we are able to image ... Full text Link to item Cite

Inability of memory T cells to induce graft-versus-host disease is a result of an abortive alloresponse.

Journal article Blood · April 1, 2007 Several groups, including our own, have independently demonstrated that effector memory T cells from non-alloantigen-primed donors do not cause graft-versus-host disease (GVHD). In the current study, we further investigated whether this approach could be e ... Full text Link to item Cite

Transplantation of vascular endothelial cells mediates the hematopoietic recovery and survival of lethally irradiated mice.

Journal article Blood · March 15, 2007 Flk-1(+) endothelial progenitors contribute critically to the definitive onset of hematopoiesis during embryogenesis. Recent studies have suggested that adult sources of endothelial cells also possess hematopoietic activity. In this study, we sought to det ... Full text Link to item Cite

345: Radioprotection by growth hormone

Conference Biology of Blood and Marrow Transplantation · February 2007 Full text Cite

Treg-cell expansion: Better to be "naive"

Journal article Blood · December 15, 2006 Full text Cite

In Vivo Radiaprotective Effects of Growth Hormone.

Conference Blood · November 16, 2006 AbstractIonizing irradiation can cause bone marrow failure and death. Very few effective therapeutic agents are currently available for this condition. It was previously published that human recombinant grow ... Full text Cite

Vascular endothelial cells produce soluble factors that mediate the recovery of human hematopoietic stem cells after radiation injury.

Journal article Biol Blood Marrow Transplant · May 2006 The risk of terrorism with nuclear or radiologic weapons is considered to be high over the coming decade. Ionizing radiation can cause a spectrum of hematologic toxicities, from mild myelosuppression to myeloablation and death. However, the potential regen ... Full text Link to item Cite

Failure of memory T cells to induce GVHD is a result of an abortive alloresponse

Conference Biology of Blood and Marrow Transplantation · February 2006 Full text Cite

Prophylaxis and treatment of acute graft-versus-host disease.

Journal article Semin Hematol · January 2006 Featured Publication Acute graft-versus-host disease (GVHD) remains a major obstacle to successful allogeneic hematopoietic stem cell transplantation (HSCT). The ability to prevent GVHD--the application of successful prophylaxis--is crucial as treatment when prophylaxis fails ... Full text Link to item Cite

The critical role of hematopoietic cells in lipopolysaccharide-induced airway inflammation.

Journal article Am J Respir Crit Care Med · April 15, 2005 Featured Publication Rapid and selective recruitment of neutrophils into the airspace in response to LPS facilitates the clearance of bacterial pathogens. However, neutrophil infiltration can also participate in the development and progression of environmental airway disease. ... Full text Link to item Cite

Mature T cells alone separate GVL from GVHD: The need for better controls

Conference Biology of Blood and Marrow Transplantation · February 2005 Full text Cite

Selective elimination of alloreactivity from immunotherapeutic T cells by photodynamic cell purging and memory T-cell sorting.

Journal article Cytotherapy · 2005 Featured Publication Allogeneic stem cell transplantation (alloSCT), especially in the mismatched setting, carries a high risk of life-threatening GvHD because of activation of donor T cells by Ag present on host cells. Removal of mature donor T cells can prevent GvHD but lead ... Full text Link to item Cite

Hematopoietic stem cell dose correlates with the speed of immune reconstitution after stem cell transplantation.

Journal article Blood · June 1, 2004 Featured Publication In the current study, we tested whether higher numbers of hematopoietic stem cells correlate with the speed of immune reconstitution in a congenic transplantation model (C57BL/Ka, CD45.1, Thy1.1-->C57BL/6, CD45.2, Thy1.2) using purified hematopoietic stem ... Full text Link to item Cite

Transfer of allogeneic CD62L- memory T cells without graft-versus-host disease.

Journal article Blood · February 15, 2004 Featured Publication The major challenge in allogeneic hematopoietic cell transplantation is how to transfer allogeneic T-cell immunity without causing graft-versus-host disease (GVHD). Here we report a novel strategy to selectively prevent GVHD by depleting CD62L(+) T cells ( ... Full text Link to item Cite

Growth hormone accelerates immune recovery following allogeneic T-cell-depleted bone marrow transplantation in mice.

Journal article Exp Hematol · October 2003 Featured Publication OBJECTIVE: To test in a murine model whether recombinant human growth hormone can promote immune recovery after allogeneic T-cell-depleted bone marrow transplantation. MATERIALS AND METHODS: Lethally irradiated (8.5 Gy) BALB/c mice (H2(d)) were transplante ... Full text Link to item Cite

Prevention of graft-versus-host disease while preserving graft-versus-leukemia effect after selective depletion of host-reactive T cells by photodynamic cell purging process.

Journal article Blood · May 1, 2002 Featured Publication In this study, we investigated the possibility of selective depletion of donor alloantigen-specific T cells from C57BL/6 (H-2(b)) mice to prevent graft-versus-host disease (GVHD). These cells were first activated with irradiated BALB/c (H-2(d)) host spleen ... Full text Link to item Cite

Immunosuppressive and antiinflammatory effects of triptolide and its prodrug PG-490-88

Journal article Drugs of the Future · March 26, 2002 Triptolide is a diterpenoid triepoxide purified from Tripterygium wilfordii Hook F, an herb found in China. Triptolide inhibits T cell activation mainly through inhibition of interleukin-2 production. In contrast to the target of ciclosporin and FK506, the ... Full text Cite

Addition of a second, different allogeneic graft accelerates white cell and platelet engraftment after T-cell-depleted bone marrow transplantation.

Journal article Blood · March 15, 2002 Featured Publication Significant delays in engraftment and lymphoid recovery are the 2 major challenges in cord blood transplantation. The cause for this delay is presumed to be the low numbers of hematopoietic precursors found in one unit of cord blood. One approach to increa ... Full text Link to item Cite

Mechanisms of tolerance induced by PG490-88 in a bone marrow transplantation model.

Journal article Transplantation · January 15, 2002 Featured Publication BACKGROUND: PG490-88, a semisynthetic derivative of a novel compound PG490 (triptolide) purified from a Chinese herb (Tripterygium wilfordii Hook F), is effective in prevention of murine graft-versus-host disease (GVHD). METHODS: PG490-88 was administrated ... Full text Link to item Cite

Nonmyeloablative regimen preserves "niches" allowing for peripheral expansion of donor T-cells.

Journal article Biol Blood Marrow Transplant · 2002 Featured Publication T-cell recovery following myeloablative preparatory regimens and cord blood transplantation in adult patients gen erally occurs between 1 and 3 years following allogeneic bone marrow transplantation. T-cell reconstitution may involve thymic education of do ... Full text Link to item Cite

A comparison of murine T-cell-depleted adult bone marrow and full-term fetal blood cells in hematopoietic engraftment and immune reconstitution.

Journal article Blood · January 1, 2002 Featured Publication Umbilical cord blood has been increasingly used as a source of hematopoietic stem cells. A major area of concern for the use of cord blood transplantation is the delay in myeloid and lymphoid recovery. To directly compare myeloid and lymphoid recovery usin ... Full text Link to item Cite

Triptolide, a novel immunosuppressive and anti-inflammatory agent purified from a Chinese herb Tripterygium wilfordii Hook F.

Journal article Leuk Lymphoma · July 2001 Featured Publication Triptolide is a diterpenoid triepoxide purified from a Chinese herb Tripterygium Wilfordii Hook F (TWHF). TWHF has been used in traditional Chinese medicine for more than two thousand years. However, its potential value was recognized by the western medici ... Full text Link to item Cite

T-Cell recovery in adults and children following umbilical cord blood transplantation.

Journal article Biol Blood Marrow Transplant · 2001 Featured Publication T-cell reconstitution following allogeneic stem cell transplantation may involve thymic education of donor-derived precursors or peripheral expansion of mature T cells transferred in the graft. T cell-receptor excision circles (sjTRECs) are generated withi ... Full text Link to item Cite

Mechanisms of tolerance induced by PG490-88 in a bone marrow transplantation model

Journal article Blood · December 1, 2000 PG490-88 is a semisyntheüc derivative of a novel compound PG490 (triptolide) purified from a Chinese herb (Tripterygium Wilfordii Hook F). Host specific tolerance in vivo was demonstrated in PG490-88-treated BALB/c recipients (H2d, Mls-21, Mls-3") of bone ... Cite

Addition of stem cells from allogeneic donors accelerates engraftment and immune reconstitution after stem cell transplantation

Journal article Blood · December 1, 2000 Umbilical cord blood is a valuable source of hematopoietic stem cells. However, stem cell numbers are limited and there are significant delays in engraftment and immune reconstitution. Since full matching at the major histocompatibility complex (MHC) appea ... Cite

Prevention of graft-versus-host disease by a novel immunosuppressant, PG490-88, through inhibition of alloreactive T cell expansion.

Journal article Transplantation · November 27, 2000 Featured Publication BACKGROUND: PG490-88 is a water soluble, semisynthetic derivative of a novel compound PG490 (triptolide) purified from the Chinese herb Tripterygium Wilfordii Hook F. METHODS: PG490-88 was administrated into recipient mice in a model (B10.D2-->BALB/c) of l ... Full text Link to item Cite

Graft-versus-host disease prevention by rapamycin: cellular mechanisms.

Journal article Biol Blood Marrow Transplant · 2000 Featured Publication Understanding the cellular mechanisms that lead to graft-versus-host disease (GVHD) may lead to alternative approaches in the prevention or therapy of this disease process. In this manuscript, we investigated the mechanisms of action of the immunosuppressi ... Full text Link to item Cite

A synthetic random basic copolymer with promiscuous binding to class II major histocompatibility complex molecules inhibits T-cell proliferative responses to major and minor histocompatibility antigens in vitro and confers the capacity to prevent murine graft-versus-host disease in vivo.

Journal article Proc Natl Acad Sci U S A · May 14, 1996 Featured Publication Graft-versus-host disease (GVHD) is a T-cell-mediated disease of transplanted donor T cells recognizing host alloantigens. Data presented in this report show, to our knowledge, for the first time that a synthetic copolymer of the amino acids L-Glu, L-Lys, ... Full text Link to item Cite