Christopher Lee Holley

Preprint · January 19, 2026 We have compared genome-wide patterns of RNA 2'-O-methylation (Nm) between two isogenic pairs of neurons. Each pair includes one line harboring a small deletion of orphan box C/D snoRNAs (SNORD116s) from the paternal chr15q11-q13 region. One isogenic pair ... Full text Link to item Cite

Genome-wide profiling of RNA 2'-O-methylation in neurons and identification of orphan snoRNA targets.

Preprint · December 17, 2025 We have compared genome-wide patterns of RNA 2'-O-methylation (Nm) between two isogenic pairs of neurons. Each pair includes one line harboring a small deletion of orphan box C/D snoRNAs (SNORD116s) from the paternal chr15q11-q13 region. One isogenic pair ... Full text Link to item Cite

snoCLASH Reveals Extensive snoRNA-mRNA Interaction Networks.

Preprint · December 11, 2025 Small nucleolar RNAs (snoRNAs) are classically defined as guides for ribosomal RNA (rRNA) modification, yet increasing evidence suggests that box C/D snoRNAs also interact with non-rRNA transcripts. Systematic discovery of such interactions has been hinder ... Full text Link to item Cite

Rpl13a snoRNAs Downregulate Smooth Muscle Cell COX4I2 and Promote Neointimal Hyperplasia.

Preprint · July 27, 2025 BACKGROUND: Reactive oxygen species (ROS) augment the activation of vascular smooth muscle cells (SMCs) and promote neointimal hyperplasia evoked by arterial injury or atherogenesis. We have previously shown that small nucleolar RNAs (snoRNAs) from the Rpl ... Full text Link to item Cite

Generating snoRNA-guided Programmable 2'- O -methylation.

Preprint · November 18, 2024 Small nucleolar RNAs (snoRNAs) are critical in guiding post-transcriptional modifications like 2'- O -methylation (Nm), which play crucial roles in downstream processes such as splicing and translation. This study tests a novel method for Nm validation, ad ... Full text Link to item Cite

Snord67 promotes lymph node metastasis and regulates U6-mediated alternative splicing in breast cancer

Preprint · 2020 Small nucleolar RNAs (snoRNAs) have long been considered “housekeeping genes”, important for ribosomal biogenesis and protein synthesis. However, there is increasing evidence that this largely ignored class of non-coding RNAs (ncRNAs) also have wide-rangin ... Full text Cite

2’- O -methylation alters the RNA secondary structural ensemble

Preprint · 2020 ABSTRACT 2’- O -methyl (Nm) is a highly abundant post-transcriptional RNA modification that plays important biological roles through mechanisms that are not entirely understood. There is evidence that Nm can alter the biological activities of RN ... Full text Cite

Extensive epitranscriptomic methylation of A and C residues on murine leukemia virus transcripts enhances viral gene expression

Preprint · 2019 While it has been known for several years that viral RNAs are subject to the addition of several distinct covalent modifications to individual nucleotides, collectively referred to as epitranscriptomic modifications, the effect of these editing events on v ... Full text Cite

Altered m ⁶ A modification of specific cellular transcripts affects Flaviviridae infection

Preprint · 2019 Summary The RNA modification N6 -methyladenosine (m 6 A) can modulate mRNA fate and thus affect many biological processes. We analyzed m 6 A modification across the transcriptome following infection by dengue virus (DENV), Zika virus (ZIKV), W ... Full text Cite