Scholars@Duke
Cristian Tudorel Badea

Cristian Tudorel Badea

Professor in Radiology
Radiology
Bryan Research Building, Room 161F, Durham, NC 27710

Scholarly Works - Journal articles

The Next Frontier in Quantitative Co-Clinical Imaging to Advance Functional Precision Oncology.

Journal article Clin Cancer Res · September 23, 2026 Precision oncology seeks to transform cancer treatment into one tailored for individual patients. Genomic alterations have been the dominant drivers of patient-specific therapeutic strategies. While genomic insights can guide treatment planning, genotype-d ... Full text Link to item Cite

Virtual photon-counting micro-CT platform for simulation of head and neck cancer imaging in mice.

Journal article Phys Med Biol · June 18, 2026 Objective.Our goal was to develop a simulation platform for photon-counting CT (PCCT) imaging in mouse models of head and neck squamous cell carcinoma (HNSCC).Approach.High-resolution vasculature from an energy-integrating detector micro-CT scan of a bariu ... Full text Link to item Cite

Photon-Counting Micro-CT for Bone Morphometry in Murine Models.

Journal article Tomography · November 13, 2025 BACKGROUND/OBJECTIVES: This study evaluates photon-counting CT (PCCT) for the imaging of mouse femurs and investigates how APOE genotype, sex, and humanized nitric oxide synthase (HN) expression influence bone morphology during aging. METHODS: A custom-bui ... Full text Link to item Cite

Image-Guided Cardiac Regeneration via a 3D Bioprinted Vascular Patch with Built-in CT Visibility.

Journal article Chem Eng J · September 15, 2025 Cardiac patch-based regenerative therapies have shown great promise in the treatment of myocardial infarction (MI). The clinical applications of patch devices, however, face major limitations mainly due to the inadequate integration of typically nonvascula ... Full text Link to item Cite

Development of anatomically variable digital Wistar rat phantoms for small animal imaging research.

Journal article Med Phys · August 2025 BACKGROUND: Digital phantoms are valuable tools for evaluating small animal imaging systems. They can help optimize imaging parameters before live studies, supporting efforts to reduce animal use and refine experimental protocols. Wistar rats are widely us ... Full text Link to item Cite

Photon-counting micro-CT scanner for deep learning-enabled small animal perfusion imaging.

Journal article Phys Med Biol · July 7, 2025 Objective.In this work, we introduce a benchtop, turn-table photon-counting (PC) micro-computed tomography (CT) scanner and highlight its application for dynamic small animal perfusion imaging.Approach.Built on recently published hardware, the system now f ... Full text Link to item Cite

Denoising pediatric cardiac photon-counting CT data with sparse coding and data-adaptive, self-supervised deep learning.

Journal article Med Phys · July 2025 BACKGROUND: The judicious use of CT in pediatric cardiac applications is warranted because young patients face the need for repeated imaging and increased lifetime cancer risk after ionizing radiation exposure. The quality of pediatric cardiac CT scans is ... Full text Link to item Cite

Nanoparticle Contrast Agents for Photon-Counting Computed Tomography: Recent Developments and Future Opportunities.

Journal article Wiley Interdiscip Rev Nanomed Nanobiotechnol · 2025 The clinical availability of photon-counting computed tomography (PCCT) has ushered in a new era of CT imaging. Spectral imaging coupled with superior contrast resolution, and ultrahigh spatial resolution (200 μm) offered by PCCT has the potential to revol ... Full text Link to item Cite

Assessing the cardioprotective effects of exercise in APOE mouse models using deep learning and photon-counting micro-CT.

Journal article PLoS One · 2025 BACKGROUND: The allelic variations of the apolipoprotein E (APOE) gene play a critical role in regulating lipid metabolism and significantly impact cardiovascular disease risk (CVD). This study aimed to evaluate the impact of exercise on cardiac structure ... Full text Link to item Cite

Exercise mitigates high-fat diet-induced cardiac dysfunction via APOE genotype- and immune-dependent mechanisms: A photon-counting CT study in adult mice.

Journal article PLoS One · 2025 BACKGROUND: Cardiovascular dysfunction frequently accompanies aging and is often worsened by adverse lifestyle factors and genetic susceptibility. The apolipoprotein E (APOE) gene modulates susceptibility to cardiovascular disease, but how exercise and die ... Full text Link to item Cite

Investigating deep learning strategies for fast denoising of 5D cardiac photon-counting micro-CT images.

Journal article Phys Med Biol · September 25, 2024 Photon-counting detectors (PCDs) for CT imaging use energy thresholds to simultaneously acquire projections at multiple energies, making them suitable for spectral imaging and material decomposition. Unfortunately, setting multiple energy thresholds r ... Full text Link to item Cite

Turn-table micro-CT scanner for dynamic perfusion imaging in mice: design, implementation, and evaluation.

Journal article Phys Med Biol · August 23, 2024 Objective.This study introduces a novel desktop micro-CT scanner designed for dynamic perfusion imaging in mice, aimed at enhancing preclinical imaging capabilities with high resolution and low radiation doses.Approach.The micro-CT system features a custom ... Full text Link to item Cite

High-resolution hybrid micro-CT imaging pipeline for mouse brain region segmentation and volumetric morphometry.

Journal article PLoS One · 2024 BACKGROUND: Brain region segmentation and morphometry in humanized apolipoprotein E (APOE) mouse models with a human NOS2 background (HN) contribute to Alzheimer's disease (AD) research by demonstrating how various risk factors affect the brain. Photon-cou ... Full text Open Access Link to item Cite

MR histology reveals tissue features beneath heterogeneous MRI signal in genetically engineered mouse models of sarcoma.

Journal article Front Oncol · 2024 PURPOSE: To identify significant relationships between quantitative cytometric tissue features and quantitative MR (qMRI) intratumorally in preclinical undifferentiated pleomorphic sarcomas (UPS). MATERIALS AND METHODS: In a prospective study of geneticall ... Full text Link to item Cite

Feature attention graph neural network for estimating brain age and identifying important neural connections in mouse models of genetic risk for Alzheimer's disease.

Journal article Imaging Neurosci (Camb) · 2024 Alzheimer's disease (AD), a widely studied neurodegenerative disorder, poses significant research challenges due to its high prevalence and complex etiology. Age, a critical risk factor for AD, is typically assessed by comparing physiological and estimated ... Full text Link to item Cite

Leveraging 3D Bioprinting and Photon-Counting Computed Tomography to Enable Noninvasive Quantitative Tracking of Multifunctional Tissue Engineered Constructs.

Journal article Adv Healthc Mater · December 2023 3D bioprinting is revolutionizing the fields of personalized and precision medicine by enabling the manufacturing of bioartificial implants that recapitulate the structural and functional characteristics of native tissues. However, the lack of quantitative ... Full text Link to item Cite

Evaluation of the impact of a novel denoising algorithm on image quality in dual-energy abdominal CT of obese patients.

Journal article Eur Radiol · October 2023 OBJECTIVES: Evaluate a novel algorithm for noise reduction in obese patients using dual-source dual-energy (DE) CT imaging. METHODS: Seventy-nine patients with contrast-enhanced abdominal imaging (54 women; age: 58 ± 14 years; BMI: 39 ± 5 kg/m2, range: 35- ... Full text Open Access Link to item Cite

MCR toolkit: A GPU-based toolkit for multi-channel reconstruction of preclinical and clinical x-ray CT data.

Journal article Med Phys · August 2023 BACKGROUND: The advancement of x-ray CT into the domains of photon counting spectral imaging and dynamic cardiac and perfusion imaging has created many new challenges and opportunities for clinicians and researchers. To address challenges such as dose cons ... Full text Open Access Link to item Cite

A Deep Learning Approach for Rapid and Generalizable Denoising of Photon-Counting Micro-CT Images.

Journal article Tomography · July 2, 2023 Photon-counting CT (PCCT) is powerful for spectral imaging and material decomposition but produces noisy weighted filtered backprojection (wFBP) reconstructions. Although iterative reconstruction effectively denoises these images, it requires extensive com ... Full text Open Access Link to item Cite

Co-Clinical Imaging Metadata Information (CIMI) for Cancer Research to Promote Open Science, Standardization, and Reproducibility in Preclinical Imaging.

Journal article Tomography · May 11, 2023 Preclinical imaging is a critical component in translational research with significant complexities in workflow and site differences in deployment. Importantly, the National Cancer Institute's (NCI) precision medicine initiative emphasizes the use of trans ... Full text Open Access Link to item Cite

Micro-CT imaging of multiple K-edge elements using GaAs and CdTe photon counting detectors.

Journal article Phys Med Biol · April 12, 2023 Objective.To evaluate the performance of two photon-counting (PC) detectors based on different detector materials, gallium arsenide (GaAs) and cadmium telluride (CdTe), for PC micro-CT imaging of phantoms with multiple contrast materials. Another objective ... Full text Open Access Link to item Cite

An Online Repository for Pre-Clinical Imaging Protocols (PIPs).

Journal article Tomography · March 27, 2023 Providing method descriptions that are more detailed than currently available in typical peer reviewed journals has been identified as an actionable area for improvement. In the biochemical and cell biology space, this need has been met through the creatio ... Full text Open Access Link to item Cite

Animal Models and Their Role in Imaging-Assisted Co-Clinical Trials.

Journal article Tomography · March 16, 2023 The availability of high-fidelity animal models for oncology research has grown enormously in recent years, enabling preclinical studies relevant to prevention, diagnosis, and treatment of cancer to be undertaken. This has led to increased opportunities to ... Full text Open Access Link to item Cite

Neoadjuvant Radiation Therapy and Surgery Improves Metastasis-Free Survival over Surgery Alone in a Primary Mouse Model of Soft Tissue Sarcoma.

Journal article Mol Cancer Ther · January 3, 2023 This study aims to investigate whether adding neoadjuvant radiotherapy (RT), anti-programmed cell death protein-1 (PD-1) antibody (anti-PD-1), or RT + anti-PD-1 to surgical resection improves disease-free survival for mice with soft tissue sarcomas (STS). ... Full text Open Access Link to item Cite

Advanced photon counting CT imaging pipeline for cardiac phenotyping of apolipoprotein E mouse models.

Journal article PLoS One · 2023 BACKGROUND: Cardiovascular disease (CVD) is associated with the apolipoprotein E (APOE) gene and lipid metabolism. This study aimed to develop an imaging-based pipeline to comprehensively assess cardiac structure and function in mouse models expressing dif ... Full text Link to item Cite

Automated multimodal segmentation of acute ischemic stroke lesions on clinical MR images.

Journal article Magn Reson Imaging · October 2022 Magnetic resonance (MR) imaging (MRI) is commonly used to diagnose, assess and monitor stroke. Accurate and timely segmentation of stroke lesions provides the anatomico-structural information that can aid physicians in predicting prognosis, as well as in d ... Full text Open Access Link to item Cite

Material decomposition from photon-counting CT using a convolutional neural network and energy-integrating CT training labels.

Journal article Phys Med Biol · July 18, 2022 Featured Work Objective.Photon-counting CT (PCCT) has better dose efficiency and spectral resolution than energy-integrating CT, which is advantageous for material decomposition. Unfortunately, the accuracy of PCCT-based material decomposition is limited due to spectral ... Full text Open Access Link to item Cite

Photon Counting CT and Radiomic Analysis Enables Differentiation of Tumors Based on Lymphocyte Burden.

Journal article Tomography · March 10, 2022 Featured Work The purpose of this study was to investigate if radiomic analysis based on spectral micro-CT with nanoparticle contrast-enhancement can differentiate tumors based on lymphocyte burden. High mutational load transplant soft tissue sarcomas were initiated in ... Full text Open Access Link to item Cite

Detection of Lung Nodules in Micro-CT Imaging Using Deep Learning.

Journal article Tomography · August 7, 2021 Featured Work We are developing imaging methods for a co-clinical trial investigating synergy between immunotherapy and radiotherapy. We perform longitudinal micro-computed tomography (micro-CT) of mice to detect lung metastasis after treatment. This work explores deep ... Full text Open Access Link to item Cite

Advances in micro-CT imaging of small animals.

Journal article Phys Med · August 2021 Featured Work PURPOSE: Micron-scale computed tomography (micro-CT) imaging is a ubiquitous, cost-effective, and non-invasive three-dimensional imaging modality. We review recent developments and applications of micro-CT for preclinical research. METHODS: Based on a comp ... Full text Open Access Link to item Cite

Evaluating renal lesions using deep-learning based extension of dual-energy FoV in dual-source CT-A retrospective pilot study.

Journal article Eur J Radiol · June 2021 PURPOSE: Dual-source (DS) CT, dual-energy (DE) field of view (FoV) is limited to the size of the smaller detector array. The purpose was to establish a deep learning-based approach to DE extrapolation by estimating missing image data using data from both t ... Full text Open Access Link to item Cite

Ex Vivo MR Histology and Cytometric Feature Mapping Connect Three-dimensional in Vivo MR Images to Two-dimensional Histopathologic Images of Murine Sarcomas.

Journal article Radiol Imaging Cancer · May 2021 Purpose To establish a platform for quantitative tissue-based interpretation of cytoarchitecture features from tumor MRI measurements. Materials and Methods In a pilot preclinical study, multicontrast in vivo MRI of murine soft-tissue sarcomas in 10 mice, ... Full text Open Access Link to item Cite

Characterization of cardiovascular injury in mice following partial-heart irradiation with clinically relevant dose and fractionation.

Journal article Radiother Oncol · April 2021 BACKGROUND AND PURPOSE: Late cardiac toxicity is a major side effect of radiation therapy (RT) for breast cancer. We developed and characterized a mouse model of radiation-induced heart disease that mimics the dose, fractionation, and beam arrangement of l ... Full text Link to item Cite

Microcephaly with altered cortical layering in GIT1 deficiency revealed by quantitative neuroimaging.

Journal article Magn Reson Imaging · February 2021 G Protein-Coupled Receptor Kinase-Interacting Protein-1 (GIT1) regulates neuronal functions, including cell and axon migration and synapse formation and maintenance, and GIT1 knockout (KO) mice exhibit learning and memory deficits. We noted that male and f ... Full text Link to item Cite

Dual source hybrid spectral micro-CT using an energy-integrating and a photon-counting detector.

Journal article Phys Med Biol · October 21, 2020 Featured Work Preclinical micro-CT provides a hotbed in which to develop new imaging technologies, including spectral CT using photon counting detector (PCD) technology. Spectral imaging using PCDs promises to expand x-ray CT as a functional imaging modality, capable of ... Full text Open Access Link to item Cite

Deep learning based spectral extrapolation for dual-source, dual-energy x-ray computed tomography.

Journal article Med Phys · September 2020 PURPOSE: Data completion is commonly employed in dual-source, dual-energy computed tomography (CT) when physical or hardware constraints limit the field of view (FoV) covered by one of two imaging chains. Practically, dual-energy data completion is accompl ... Full text Open Access Link to item Cite

Co-Clinical Imaging Resource Program (CIRP): Bridging the Translational Divide to Advance Precision Medicine.

Journal article Tomography · September 2020 The National Institutes of Health's (National Cancer Institute) precision medicine initiative emphasizes the biological and molecular bases for cancer prevention and treatment. Importantly, it addresses the need for consistency in preclinical and clinical ... Full text Open Access Link to item Cite

MRI-Based Deep Learning Segmentation and Radiomics of Sarcoma in Mice.

Journal article Tomography · March 2020 Featured Work Small-animal imaging is an essential tool that provides noninvasive, longitudinal insight into novel cancer therapies. However, considerable variability in image analysis techniques can lead to inconsistent results. We have developed quantitative imaging f ... Full text Open Access Link to item Cite

The impact of respiratory gating on improving volume measurement of murine lung tumors in micro-CT imaging.

Journal article PLoS One · 2020 Small animal imaging has become essential in evaluating new cancer therapies as they are translated from the preclinical to clinical domain. However, preclinical imaging faces unique challenges that emphasize the gap between mouse and man. One example is t ... Full text Open Access Link to item Cite

The impact of respiratory gating on improving volume measurement of murine lung tumors in micro-CT imaging

Journal article · October 29, 2019 ABSTRACT Small animal imaging has become essential in evaluating new cancer therapies as they are translated from the preclinical to clinical domain. However, preclinical imaging faces uni ... Full text Open Access Cite

Sensitization of Vascular Endothelial Cells to Ionizing Radiation Promotes the Development of Delayed Intestinal Injury in Mice.

Journal article Radiat Res · September 2019 Exposure of the gastrointestinal (GI) tract to ionizing radiation can cause acute and delayed injury. However, critical cellular targets that regulate the development of radiation-induced GI injury remain incompletely understood. Here, we investigated the ... Full text Open Access Link to item Cite

Photon-counting cine-cardiac CT in the mouse

Journal article · June 4, 2019 Abstract The maturation of photon-counting detector (PCD) technology promises to enhance routine CT imaging applications with high-fidelity spectral information. In this paper, we demonstrate the power of this synergy and our complementary reconstruction t ... Full text Cite

Vascular Endothelial Growth Factor Receptor 3 Regulates Endothelial Function Through β-Arrestin 1.

Journal article Circulation · March 26, 2019 BACKGROUND: Receptor signaling is central to vascular endothelial function and is dysregulated in vascular diseases such as atherosclerosis and pulmonary arterial hypertension (PAH). Signaling pathways involved in endothelial function include vascular endo ... Full text Link to item Cite

Functional imaging of tumor vasculature using iodine and gadolinium-based nanoparticle contrast agents: a comparison of spectral micro-CT using energy integrating and photon counting detectors.

Journal article Phys Med Biol · March 12, 2019 Advances in computed tomography (CT) hardware have propelled the development of novel CT contrast agents. In particular, the spectral capabilities of x-ray CT can facilitate simultaneous imaging of multiple contrast agents. This approach is particularly us ... Full text Open Access Link to item Cite

Overcoming detector limitations of x-ray photon counting for preclinical microcomputed tomography.

Journal article J Med Imaging (Bellingham) · January 2019 Spectral computed tomography (CT) using photon counting detectors (PCDs) can provide accurate tissue composition measurements by utilizing the energy dependence of x-ray attenuation in different materials. PCDs are especially suited for K-edge imaging, rev ... Full text Open Access Link to item Cite

Bridging the translational gap: Implementation of multimodal small animal imaging strategies for tumor burden assessment in a co-clinical trial.

Journal article PLoS One · 2019 Featured Work In designing co-clinical cancer studies, preclinical imaging brings unique challenges that emphasize the gap between man and mouse. Our group is developing quantitative imaging methods for the preclinical arm of a co-clinical trial studying immunotherapy a ... Full text Open Access Link to item Cite

Photon-counting cine-cardiac CT in the mouse.

Journal article PLoS One · 2019 Featured Work The maturation of photon-counting detector (PCD) technology promises to enhance routine CT imaging applications with high-fidelity spectral information. In this paper, we demonstrate the power of this synergy and our complementary reconstruction techniques ... Full text Open Access Link to item Cite

Low-dose 4D cardiac imaging in small animals using dual source micro-CT.

Journal article Phys Med Biol · January 9, 2018 Micro-CT is widely used in preclinical studies, generating substantial interest in extending its capabilities in functional imaging applications such as blood perfusion and cardiac function. However, imaging cardiac structure and function in mice is challe ... Full text Open Access Link to item Cite

Dual-Energy CT Imaging of Tumor Liposome Delivery After Gold Nanoparticle-Augmented Radiation Therapy.

Journal article Theranostics · 2018 Featured Work Gold nanoparticles (AuNPs) are emerging as promising agents for both cancer therapy and computed tomography (CT) imaging. AuNPs absorb x-rays and subsequently release low-energy, short-range photoelectrons during external beam radiation therapy (RT), incre ... Full text Open Access Link to item Cite

A comparative analysis of EGFR-targeting antibodies for gold nanoparticle CT imaging of lung cancer.

Journal article PLoS One · 2018 Computed tomography (CT) is the standard imaging test used for the screening and assessment of suspected lung cancer, but distinguishing malignant from benign nodules by CT is an ongoing challenge. Consequently, a large number of avoidable invasive procedu ... Full text Open Access Link to item Cite

Bridging the translational gap: Implementation of multimodal small animal imaging strategies for tumor burden assessment in a co-clinical trial

Journal article · 2018 In designing co-clinical cancer studies, preclinical imaging brings unique challenges that emphasize the gap between man and mouse. Our group is developing quantitative imaging methods for the preclinical arm of a co-clinical trial studying immunotherapy a ... Full text Cite

Hybrid spectral CT reconstruction.

Journal article PLoS One · 2017 Current photon counting x-ray detector (PCD) technology faces limitations associated with spectral fidelity and photon starvation. One strategy for addressing these limitations is to supplement PCD data with high-resolution, low-noise data acquired with an ... Full text Open Access Link to item Cite

A neural network-based method for spectral distortion correction in photon counting x-ray CT.

Journal article Phys Med Biol · August 21, 2016 Spectral CT using a photon counting x-ray detector (PCXD) shows great potential for measuring material composition based on energy dependent x-ray attenuation. Spectral CT is especially suited for imaging with K-edge contrast agents to address the otherwis ... Full text Open Access Link to item Cite

Spectrotemporal CT data acquisition and reconstruction at low dose.

Journal article Med Phys · November 2015 Featured Work PURPOSE: X-ray computed tomography (CT) is widely used, both clinically and preclinically, for fast, high-resolution anatomic imaging; however, compelling opportunities exist to expand its use in functional imaging applications. For instance, spectral info ... Full text Open Access Link to item Cite

SU-C-303-07: Influence of Image Registration Algorithms and Noise Levels On the Accuracy of Fractional Regional Ventilation.

Journal article Med Phys · June 2015 PURPOSE: The derivation of fractional regional lung-ventilation (FRV) involves registering images corresponding to extreme phases of the respiratory cycle. The purpose of this work was to investigate the influence of registration algorithms and image noise ... Full text Link to item Cite

A Plasmonic Gold Nanostar Theranostic Probe for In Vivo Tumor Imaging and Photothermal Therapy.

Journal article Theranostics · 2015 Nanomedicine has attracted increasing attention in recent years, because it offers great promise to provide personalized diagnostics and therapy with improved treatment efficacy and specificity. In this study, we developed a gold nanostar (GNS) probe for m ... Full text Open Access Link to item Cite

Localization of Metal Electrodes in the Intact Rat Brain Using Registration of 3D Microcomputed Tomography Images to a Magnetic Resonance Histology Atlas.

Journal article eNeuro · 2015 Simultaneous neural recordings taken from multiple areas of the rodent brain are garnering growing interest due to the insight they can provide about spatially distributed neural circuitry. The promise of such recordings has inspired great progress in meth ... Full text Open Access Link to item Cite

In vivo small animal micro-CT using nanoparticle contrast agents.

Journal article Front Pharmacol · 2015 Featured Work Computed tomography (CT) is one of the most valuable modalities for in vivo imaging because it is fast, high-resolution, cost-effective, and non-invasive. Moreover, CT is heavily used not only in the clinic (for both diagnostics and treatment planning) but ... Full text Open Access Link to item Cite

Spectral diffusion: an algorithm for robust material decomposition of spectral CT data.

Journal article Phys Med Biol · November 7, 2014 Clinical successes with dual energy CT, aggressive development of energy discriminating x-ray detectors, and novel, target-specific, nanoparticle contrast agents promise to establish spectral CT as a powerful functional imaging modality. Common to all of t ... Full text Open Access Link to item Cite

Micro-CT of rodents: state-of-the-art and future perspectives.

Journal article Phys Med · September 2014 Featured Work Micron-scale computed tomography (micro-CT) is an essential tool for phenotyping and for elucidating diseases and their therapies. This work is focused on preclinical micro-CT imaging, reviewing relevant principles, technologies, and applications. Commonly ... Full text Open Access Link to item Cite

Comparison of 4D-microSPECT and microCT for murine cardiac function.

Journal article Mol Imaging Biol · April 2014 PURPOSE: The objective of this study was to compare a new generation of four-dimensional micro-single photon emission computed tomography (microSPECT) with microCT for the quantitative in vivo assessment of murine cardiac function. PROCEDURES: Four-dimensi ... Full text Link to item Cite

Assessing cardiac injury in mice with dual energy-microCT, 4D-microCT, and microSPECT imaging after partial heart irradiation.

Journal article Int J Radiat Oncol Biol Phys · March 1, 2014 Featured Work PURPOSE: To develop a mouse model of cardiac injury after partial heart irradiation (PHI) and to test whether dual energy (DE)-microCT and 4-dimensional (4D)-microCT can be used to assess cardiac injury after PHI to complement myocardial perfusion imaging ... Full text Open Access Link to item Cite

Dual-energy micro-CT functional imaging of primary lung cancer in mice using gold and iodine nanoparticle contrast agents: a validation study.

Journal article PLoS One · 2014 Featured Work PURPOSE: To provide additional functional information for tumor characterization, we investigated the use of dual-energy computed tomography for imaging murine lung tumors. Tumor blood volume and vascular permeability were quantified using gold and iodine ... Full text Open Access Link to item Cite

Anatomical and functional imaging of myocardial infarction in mice using micro-CT and eXIA 160 contrast agent.

Journal article Contrast Media Mol Imaging · 2014 Featured Work Noninvasive small animal imaging techniques are essential for evaluation of cardiac disease and potential therapeutics. A novel preclinical iodinated contrast agent called eXIA 160 has recently been developed, which has been evaluated for micro-CT cardiac ... Full text Link to item Cite

Constructing a 4D murine cardiac micro-CT atlas for automated segmentation and phenotyping applications

Journal article Progress in Biomedical Optics and Imaging Proceedings of SPIE · June 3, 2013 A number of investigators have demonstrated the potential of preclinical micro-CT in characterizing cardiovascular disease in mouse models. One major hurdle to advancing this approach is the extensive user interaction required to derive quantitative metric ... Full text Cite

WE-G-134-08: Extracting Fractional Regional Ventilation in Mice.

Journal article Med Phys · June 2013 PURPOSE: Testing altered treatment paradigms, prospectively in cohorts of patients remains challenging due to the large variability in tumor characteristics and underlying comorbidities. Preclinical models of lung cancer can enable such studies in a contro ... Full text Link to item Cite

Dual-energy micro-computed tomography imaging of radiation-induced vascular changes in primary mouse sarcomas.

Journal article Int J Radiat Oncol Biol Phys · April 1, 2013 Featured Work PURPOSE: To evaluate the effects of radiation therapy on primary tumor vasculature using dual-energy (DE) micro-computed tomography (micro-CT). METHODS AND MATERIALS: Primary sarcomas were generated with mutant Kras and p53. Unirradiated tumors were compar ... Full text Link to item Cite

In vivo characterization of tumor vasculature using iodine and gold nanoparticles and dual energy micro-CT.

Journal article Phys Med Biol · March 21, 2013 Featured Work Tumor blood volume and vascular permeability are well established indicators of tumor angiogenesis and important predictors in cancer diagnosis, planning and treatment. In this work, we establish a novel preclinical imaging protocol which allows quantitati ... Full text Link to item Cite

Dual-energy computed tomography imaging of atherosclerotic plaques in a mouse model using a liposomal-iodine nanoparticle contrast agent.

Journal article Circ Cardiovasc Imaging · March 1, 2013 BACKGROUND: The accumulation of macrophages in inflamed atherosclerotic plaques has long been recognized. In an attempt to develop an imaging agent for detection of vulnerable plaques, we evaluated the feasibility of a liposomal-iodine nanoparticle contras ... Full text Link to item Cite

A LabVIEW Platform for Preclinical Imaging Using Digital Subtraction Angiography and Micro-CT.

Journal article J Med Eng · 2013 CT and digital subtraction angiography (DSA) are ubiquitous in the clinic. Their preclinical equivalents are valuable imaging methods for studying disease models and treatment. We have developed a dual source/detector X-ray imaging system that we have used ... Full text Open Access Link to item Cite

Assessing the radiation response of lung cancer with different gene mutations using genetically engineered mice.

Journal article Front Oncol · 2013 PURPOSE: Non-small cell lung cancers (NSCLC) are a heterogeneous group of carcinomas harboring a variety of different gene mutations. We have utilized two distinct genetically engineered mouse models of human NSCLC (adenocarcinoma) to investigate how genet ... Full text Open Access Link to item Cite

Registration-based segmentation of murine 4D cardiac micro-CT data using symmetric normalization.

Journal article Phys Med Biol · October 7, 2012 Featured Work Micro-CT can play an important role in preclinical studies of cardiovascular disease because of its high spatial and temporal resolution. Quantitative analysis of 4D cardiac images requires segmentation of the cardiac chambers at each time point, an extrem ... Full text Link to item Cite

Temporal and spectral imaging with micro-CT.

Journal article Med Phys · August 2012 Featured Work PURPOSE: Micro-CT is widely used for small animal imaging in preclinical studies of cardiopulmonary disease, but further development is needed to improve spatial resolution, temporal resolution, and material contrast. We present a technique for visualizing ... Full text Link to item Cite

Dual-energy micro-CT of the rodent lung.

Journal article Am J Physiol Lung Cell Mol Physiol · May 15, 2012 Featured Work The purpose of this work is to investigate the use of dual-energy micro-computed tomography (CT) for the estimation of vascular, tissue, and air fractions in rodent lungs using a postreconstruction three material decomposition method. Using simulations, we ... Full text Link to item Cite

A spectral calibration technique for x-ray CT

Journal article Progress in Biomedical Optics and Imaging Proceedings of SPIE · May 4, 2012 Spectral reconstruction algorithms for x-ray CT require an accurate spectral model of the system, including the spectrum of the photons emitted by the source and the spectral sensitivity of the detector. Although these components of the spectral model have ... Full text Cite

Lung imaging in rodents using dual energy micro-CT.

Journal article Proc SPIE Int Soc Opt Eng · April 3, 2012 Dual energy CT imaging is expected to play a major role in the diagnostic arena as it provides material decomposition on an elemental basis. The purpose of this work is to investigate the use of dual energy micro-CT for the estimation of vascular, tissue, ... Full text Link to item Cite

4D micro-CT using fast prospective gating.

Journal article Phys Med Biol · January 7, 2012 Featured Work Micro-CT is currently used in preclinical studies to provide anatomical information. But, there is also significant interest in using this technology to obtain functional information. We report here a new sampling strategy for 4D micro-CT for functional ca ... Full text Link to item Cite

Computed tomography imaging of primary lung cancer in mice using a liposomal-iodinated contrast agent.

Journal article PLoS One · 2012 Featured Work PURPOSE: To investigate the utility of a liposomal-iodinated nanoparticle contrast agent and computed tomography (CT) imaging for characterization of primary nodules in genetically engineered mouse models of non-small cell lung cancer. METHODS: Primary lun ... Full text Open Access Link to item Cite

Data analysis: evaluation of nanoscale contrast agent enhanced CT scan to differentiate between benign and malignant lung cancer in mouse model.

Journal article AMIA Annu Symp Proc · 2012 Proposed is a method for statistical analysis for a small sample size, repeated measure experiment with nesting factors. In the original experiment the Student t-test was used for analysis. Using the same data, we modeled the experiment into two groups of ... Link to item Cite

High-resolution reconstruction of fluorescent inclusions in mouse thorax using anatomically guided sampling and parallel Monte Carlo computing.

Journal article Biomed Opt Express · September 1, 2011 Featured Work We present a method for high-resolution reconstruction of fluorescent images of the mouse thorax. It features an anatomically guided sampling method to retrospectively eliminate problematic data and a parallel Monte Carlo software package to compute the Ja ... Full text Open Access Link to item Cite

4D micro-CT for cardiac and perfusion applications with view under sampling.

Journal article Phys Med Biol · June 7, 2011 Featured Work Micro-CT is commonly used in preclinical studies to provide anatomical information. There is growing interest in obtaining functional measurements from 4D micro-CT. We report here strategies for 4D micro-CT with a focus on two applications: (i) cardiac ima ... Full text Link to item Cite

Multi-modality PET-CT imaging of breast cancer in an animal model using nanoparticle x-ray contrast agent and 18F-FDG

Journal article Progress in Biomedical Optics and Imaging Proceedings of SPIE · May 16, 2011 Multi-modality PET-CT imaging is playing an important role in the field of oncology. While PET imaging facilitates functional interrogation of tumor status, the use of CT imaging is primarily limited to anatomical reference. In an attempt to extract compre ... Full text Cite

Temporal and spectral reconstruction algorithms for x-ray CT

Journal article Progress in Biomedical Optics and Imaging Proceedings of SPIE · May 13, 2011 X-ray CT imaging of dynamic physiological processes entails the reconstruction of volumetric images of objects with x-ray attenuation properties that vary over time and energy. We show how the same algebraic model can be used to represent both temporal and ... Full text Cite

Highly efficient detection in fluorescence tomography of quantum dots using time-gated acquisition and ultrafast pulsed laser.

Journal article Proc SPIE Int Soc Opt Eng · January 23, 2011 Quantum dots (QDs) are widely used in fluorescence tomography due to its unique advantages. Despite the very high quantum efficiency of the QDs, low fluorescent signal and autofluorescence are the most fundamental limitations in optical data acquisition. T ... Full text Open Access Link to item Cite

Evaluation of tumor microenvironment in an animal model using a nanoparticle contrast agent in computed tomography imaging.

Journal article Acad Radiol · January 2011 Featured Work RATIONALE AND OBJECTIVES: Non-invasive longitudinal imaging of tumor vasculature could provide new insights into the development of solid tumors, facilitating efficient delivery of therapeutics. In this study, we report three-dimensional imaging and charac ... Full text Link to item Cite

Phenylephrine-modulated cardiopulmonary blood flow measured with use of X-ray digital subtraction angiography.

Journal article J Pharmacol Toxicol Methods · 2011 Featured Work INTRODUCTION: Cardiopulmonary blood flow is an important indicator of organ function. Limitations in measuring blood flow in live rodents suggest that rapid physiological changes may be overlooked. For instance, relative measurements limit imaging to whole ... Full text Link to item Cite

In vivo imaging of rat coronary arteries using bi-plane digital subtraction angiography.

Journal article J Pharmacol Toxicol Methods · 2011 Featured Work INTRODUCTION: X-ray based digital subtraction angiography (DSA) is a common clinical imaging method for vascular morphology and function. Coronary artery characterization is one of its most important applications. We show that bi-plane DSA of rat coronary ... Full text Link to item Cite

Micro-CT imaging assessment of dobutamine-induced cardiac stress in rats.

Journal article J Pharmacol Toxicol Methods · 2011 Featured Work INTRODUCTION: Dobutamine (DOB) stress in animal models of heart disease has been imaged so far using echocardiography and magnetic resonance imaging. The purpose of this study was to assess normal response to DOB stress in rats using anatomical and functio ... Full text Link to item Cite

Phase-selective image reconstruction of the lungs in small animals using Micro-CT.

Journal article Proc SPIE Int Soc Opt Eng · February 15, 2010 Featured Work Gating in small animal imaging can compensate for artifacts due to physiological motion. This paper presents a strategy for sampling and image reconstruction in the rodent lung using micro-CT. The approach involves rapid sampling of free-breathing mice wit ... Full text Link to item Cite

Lung perfusion imaging in small animals using 4D micro-CT at heartbeat temporal resolution.

Journal article Med Phys · January 2010 Featured Work PURPOSE: Quantitative in vivo imaging of lung perfusion in rodents can provide critical information for preclinical studies. However, the combined challenges of high temporal and spatial resolution have made routine quantitative perfusion imaging difficult ... Full text Link to item Cite

Micro-CT imaging of breast tumors in rodents using a liposomal, nanoparticle contrast agent

Journal article International Journal of Nanomedicine · November 2009 Full text Cite

Quantitative blood flow measurements in the small animal cardiopulmonary system using digital subtraction angiography.

Journal article Med Phys · November 2009 Featured Work PURPOSE: The use of preclinical rodent models of disease continues to grow because these models help elucidate pathogenic mechanisms and provide robust test beds for drug development. Among the major anatomic and physiologic indicators of disease progressi ... Full text Link to item Cite

ZINC SULFIDE FINE PARTICLES OBTAINED AT LOW TEMPERATURE

Journal article CHALCOGENIDE LETTERS · September 1, 2009 Link to item Cite

Effects of sampling strategy on image quality in noncontact panoramic fluorescence diffuse optical tomography for small animal imaging.

Journal article Opt Express · March 30, 2009 Featured Work Fluorescence diffuse optical tomography is an emerging technology for molecular imaging with recent technological advances in biomarkers and photonics. The introduction of noncontact imaging methods enables very large-scale data acquisition that is orders ... Full text Link to item Cite

Three-dimensional reconstruction in free-space whole-body fluorescence tomography of mice using optically reconstructed surface and atlas anatomy.

Journal article J Biomed Opt · 2009 We present a 3-D image reconstruction method for free-space fluorescence tomography of mice using hybrid anatomical prior information. Specifically, we use an optically reconstructed surface of the experimental animal and a digital mouse atlas to approxima ... Full text Link to item Cite

Micro-CT imaging of breast tumors in rodents using a liposomal, nanoparticle contrast agent.

Journal article Int J Nanomedicine · 2009 Featured Work A long circulating liposomal, nanoscale blood pool agent encapsulating traditional iodinated contrast agent (65 mg I/mL) was used for micro-computed tomography (CT) imaging of rats implanted with R3230AC mammary carcinoma. Three-dimensional vascular archit ... Open Access Link to item Cite

In vivo small-animal imaging using micro-CT and digital subtraction angiography.

Journal article Phys Med Biol · October 7, 2008 Small-animal imaging has a critical role in phenotyping, drug discovery and in providing a basic understanding of mechanisms of disease. Translating imaging methods from humans to small animals is not an easy task. The purpose of this work is to review in ... Full text Link to item Cite

A micro-CT analysis of murine lung recruitment in bleomycin-induced lung injury.

Journal article J Appl Physiol (1985) · August 2008 The effects of lung injury on pulmonary recruitment are incompletely understood. X-ray computed tomography (CT) has been a valuable tool in assessing changes in recruitment during lung injury. With the development of preclinical CT scanners designed for th ... Full text Link to item Cite

Utility of a prototype liposomal contrast agent for x-ray imaging of breast cancer: A proof of concept using micro-CT in small animals

Journal article Progress in Biomedical Optics and Imaging Proceedings of SPIE · May 14, 2008 Imaging tumor angiogenesis in small animals is extremely challenging due to the size of the tumor vessels. Consequently, both dedicated small animal imaging systems and specialized intravascular contrast agents are required. The goal of this study was to i ... Full text Cite

Optimization of dual energy contrast enhanced breast tomosynthesis for improved mammographic lesion detection and diagnosis

Journal article Progress in Biomedical Optics and Imaging Proceedings of SPIE · May 14, 2008 Dual-energy contrast-enhanced breast tomosynthesis has been proposed as a technique to improve the detection of early-stage cancer in young, high-risk women. This study focused on optimizing this technique using computer simulations. The computer simulatio ... Full text Cite

Geometric calibration for a dual tube/detector micro-CT system.

Journal article Med Phys · May 2008 The authors describe a dual tube/detector micro-computed tomography (micro-CT) system that has the potential to improve temporal resolution and material contrast in small animal imaging studies. To realize this potential, it is necessary to precisely calib ... Full text Link to item Cite

Left ventricle volume measurements in cardiac micro-CT: the impact of radiation dose and contrast agent.

Journal article Comput Med Imaging Graph · April 2008 Micro-CT-based cardiac function estimation in small animals requires measurement of left ventricle (LV) volume at multiple time points during the cardiac cycle. Measurement accuracy depends on the image resolution, its signal and noise properties, and the ... Full text Link to item Cite

A registration based approach for 4D cardiac micro-CT using combined prospective and retrospective gating.

Journal article Med Phys · April 2008 Recent advances in murine cardiac studies with three-dimensional cone beam micro-computed tomography (CT) have used either prospective or retrospective gating technique. While prospective gating ensures the best image quality and the highest resolution, it ... Full text Link to item Cite

A high-precision contrast injector for small animal x-ray digital subtraction angiography.

Journal article IEEE Trans Biomed Eng · March 2008 Featured Work The availability of genetically altered animal models of human disease for basic research has generated great interest in new imaging methodologies. Digital subtraction angiography (DSA) offers an appealing approach to functional imaging in small animals b ... Full text Link to item Cite

Sparseness prior based iterative image reconstruction for retrospectively gated cardiac micro-CT.

Journal article Med Phys · November 2007 Featured Work Recent advances in murine cardiac studies with three-dimensional (3D) cone beam micro-CT used a retrospective gating technique. However, this sampling technique results in a limited number of projections with an irregular angular distribution due to the te ... Full text Link to item Cite

High-resolution imaging of murine myocardial infarction with delayed-enhancement cine micro-CT.

Journal article Am J Physiol Heart Circ Physiol · June 2007 Featured Work The objective of this study was to determine the feasibility of delayed-enhancement micro-computed tomography (microCT) imaging to quantify myocardial infarct size in experimental mouse models. A total of 20 mice were imaged 5 or 35 days after surgical lig ... Full text Link to item Cite

Tomographic digital subtraction angiography for lung perfusion estimation in rodents.

Journal article Med Phys · May 2007 Featured Work In vivo measurements of perfusion present a challenge to existing small animal imaging techniques such as magnetic resonance microscopy, micro computed tomography, micro positron emission tomography, and microSPECT, due to combined requirements for high sp ... Full text Link to item Cite

A micro-computed tomography-based method for the measurement of pulmonary compliance in healthy and bleomycin-exposed mice.

Journal article Exp Lung Res · 2007 Micro-computed tomography (microCT) is being increasingly used to examine small animal models of pulmonary injury. The authors have developed a microCT technique suitable for the determination of pulmonary compliance in injured mice. Lung volumes in normal ... Full text Link to item Cite

Cardiac micro-computed tomography for morphological and functional phenotyping of muscle LIM protein null mice.

Journal article Mol Imaging · 2007 The purpose of this study was to investigate the use of micro-computed tomography (micro-CT) for morphological and functional phenotyping of muscle LIM protein (MLP) null mice and to compare micro-CT with M-mode echocardiography. MLP null mice and controls ... Link to item Cite

Optimized radiographic spectra for small animal digital subtraction angiography.

Journal article Med Phys · November 2006 Featured Work The increasing use of small animals in basic research has spurred interest in new imaging methodologies. Digital subtraction angiography (DSA) offers a particularly appealing approach to functional imaging in the small animal. This study examines the optim ... Full text Link to item Cite

Three-dimensional imaging of xenograft tumors using optical computed and emission tomography.

Journal article Med Phys · September 2006 Featured Work The physical basis and preliminary applications of optical computed tomography (optical-CT) and optical emission computed tomography (optical-ECT) are introduced, as new techniques with potential to provide unique 3D information on a variety of aspects of ... Full text Link to item Cite

A liposomal nanoscale contrast agent for preclinical CT in mice.

Journal article AJR Am J Roentgenol · February 2006 Featured Work OBJECTIVE: The goal of this study was to determine if an iodinated, liposomal contrast agent could be used for high-resolution, micro-CT of low-contrast, small-size vessels in a murine model. MATERIALS AND METHODS: A second-generation, liposomal blood pool ... Full text Link to item Cite

Tumor imaging in small animals with a combined micro-CT/micro-DSA system using iodinated conventional and blood pool contrast agents.

Journal article Contrast Media Mol Imaging · 2006 Featured Work X-ray based micro-computed tomography (CT) and micro-digital subtraction angiography (DSA) are important non-invasive imaging modalities for following tumorogenesis in small animals. To exploit these imaging capabilities further, the two modalities were co ... Full text Link to item Cite

Imaging methods for morphological and functional phenotyping of the rodent heart.

Journal article Toxicol Pathol · 2006 Featured Work Small animal imaging has a critical role in phenotyping, drug discovery, and in providing a basic understanding of mechanisms of disease. Translating imaging methods from humans to small animals is not an easy task. The purpose of this work is to compare t ... Full text Link to item Cite

Development and evaluation of an ODL course on Medical Image Processing.

Journal article Med Eng Phys · September 2005 Recent developments in the field of Biomedical Engineering have led to considerable improvement in health care delivery, but also impose continuous change and improvement in education and training schemes of its professionals, in order to assure appropriat ... Full text Link to item Cite

Effects of breathing and cardiac motion on spatial resolution in the microscopic imaging of rodents.

Journal article Magn Reson Med · April 2005 Featured Work One can acquire high-resolution pulmonary and cardiac images in live rodents with MR microscopy by synchronizing the image acquisition to the breathing cycle across multiple breaths, and gating to the cardiac cycle. The precision with which one can synchro ... Full text Link to item Cite

4-D micro-CT of the mouse heart.

Journal article Mol Imaging · 2005 Featured Work PURPOSE: Demonstrate noninvasive imaging methods for in vivo characterization of cardiac structure and function in mice using a micro-CT system that provides high photon fluence rate and integrated motion control. MATERIALS AND METHODS: Simultaneous cardia ... Full text Link to item Cite

Micro-CT with respiratory and cardiac gating.

Journal article Med Phys · December 2004 Featured Work Cardiopulmonary imaging in rodents using micro-computed tomography (CT) is a challenging task due to both cardiac and pulmonary motion and the limited fluence rate available from micro-focus x-ray tubes of most commercial systems. Successful imaging in the ... Full text Link to item Cite

Experiments with the nonlinear and chaotic behaviour of the multiplicative algebraic reconstruction technique (MART) algorithm for computed tomography.

Journal article Phys Med Biol · April 21, 2004 Featured Work Among the iterative reconstruction algorithms for tomography, the multiplicative algebraic reconstruction technique (MART) has two advantages that make it stand out from other algorithms: it confines the image (and therefore the projection data) to the con ... Full text Link to item Cite

Estimation of the heart respiratory motion with applications for cone beam computed tomography imaging: a simulation study.

Journal article IEEE Trans Inf Technol Biomed · December 2003 Featured Work Computed tomography (CT) reconstruction methods assume imaging of static objects; object movement during projection data acquisition causes tomogram artifacts. The continuously moving heart, therefore, represents a complicated imaging case. The associated ... Full text Link to item Cite

A novel approach for distortion correction for X-ray image intensifiers.

Journal article Comput Med Imaging Graph · 2003 Featured Work Applications of X-ray image intensifiers in medical imaging, include the use of fluoroscopic projection images to generate three-dimensional tomographic reconstructions. Unfortunately, the inherent distortions on the acquired projections deteriorate the qu ... Full text Link to item Cite

Respiratory displacement modelling in cone beam computed tomography

Journal article Computers in Cardiology · January 1, 2003 Any organ motion during the projection acquisition causes blurring artefacts in the Computed Tomography (CT) reconstructions. It is a drawback in applications of Cone Beam CT (CBCT) for which, the complete scanning cannot be performed during a single breat ... Full text Cite

Logarithmic amplifier for computed tomography tasks using fluoroscopic projections.

Journal article J Med Eng Technol · 2002 Featured Work The image intensifier (II)-based imaging systems, as radiotherapy simulators or C-arm X-ray units, have also been used for image acquisition in computed tomography. When analogue-to-digital conversion is performed on the output signal of the television cam ... Full text Link to item Cite

Image quality in extended arc filtered digital tomosynthesis.

Journal article Acta Radiol · March 2001 Featured Work PURPOSE: To study image quality in filtered digital tomosynthesis (FDTS) tomograms as a function of their reconstruction arc, using isocentrically acquired, fluoroscopic projection data. MATERIAL AND METHODS: Both digital tomosynthesis (DTS) and cone beam ... Full text Link to item Cite

Three-dimensional localisation based on projectional and tomographic image correlation: an application for digital tomosynthesis.

Journal article Med Eng Phys · March 1999 Featured Work Accurate three-dimensional tumor localisation in Radiotherapy, is critical to the treatment outcome, particularly when high dose gradients are present. A number of techniques have been proposed for the localisation of anatomical structures or markers. The ... Full text Link to item Cite

A software tool to assist the planning of electrophysiological experiments on rat brain slices

Journal article Studies in Health Technology and Informatics · January 1, 1999 We propose an interactive software tool for finding the plane which can be cut through the rat brain so as to contain maximum cross-section with a selected brain structure or combination of structures, i.e. a path of nerve fibers and the area they are know ... Full text Cite

A wavelet-based method for removal of out-of-plane structures in digital tomosynthesis.

Journal article Comput Med Imaging Graph · 1998 Featured Work Reconstructed images in digital tomosynthesis (DTS) are affected by artifacts due to blur from planes other than the fulcrum plane. A wavelet-based method has been developed for the discrimination and subsequent removal of unrelated structures from the rec ... Full text Link to item Cite

427A localization tool on the basis of digital tomosynthesis

Journal article Radiotherapy and Oncology · January 1996 Full text Cite

Medical device surveillance world-wide.

Journal article Stud Health Technol Inform · 1996 Featured Work Link to item Cite