Daniel Julio Lew | Scholars@Duke

Daniel Julio Lew
James B. Duke Professor of Pharmacology and Cancer Biology

Our research interests encompass questions on cell cycle control, the control of cell polarity, and the specification of distinct cortical domains within cells. We are also trying to understand how cells can monitor their shape and react to environmental influences that affect cytoskeletal behavior.
One focus is the study of how the Cyclin Dependent Kinases (CDKs) that control cell cycle progression act to promote specific changes in cell polarity. A ras-related G protein, Cdc42p, is key for enacting changes in cell polarity involving reorganization of both actin and septins (a poorly understood filamentous system that specializes specific cortical domains) in response to CDK activity. We are tracing the links between the CDK and Cdc42p to understand how polarity is established, and the links between Cdc42p and the cytoskeleton to determine how polarized behavior is executed.
A second focus involves investigation of a cell cycle checkpoint control that monitors cell shape. When environmental insults disrupt cytoskeletal organization, this checkpoint delays entry into mitosis through inhibition of CDK/cyclin kinases. A tyrosine kinase, Swe1p, is responsible for the cell cycle block, and we have found that the degradation of Swe1p is regulated both by cell shape and by perturbation of the actin cytoskeleton. Recently, we discovered that the septin cytoskeleton is directly affected by local cell shape, and that proteins controlling Swe1p degradation can monitor this septin change.
The biological problems we address are universal, and the proteins that we study are widely conserved. We have chosen the experimentally tractable budding yeast as our experimental system and are using genetic, cell biological, and biochemical approaches to study these pathways.

Current Research Interests

Cell polarity
Gradient sensing
Mating
Cell Cycle

Current Appointments & Affiliations

James B. Duke Professor of Pharmacology and Cancer Biology, Pharmacology & Cancer Biology, Basic Science Departments 2011
Professor of Pharmacology and Cancer Biology, Pharmacology & Cancer Biology, Basic Science Departments 2006
Professor in Molecular Genetics and Microbiology, Molecular Genetics and Microbiology, Basic Science Departments 2008
Professor of Cell Biology, Cell Biology, Basic Science Departments 2009
Member of the Duke Cancer Institute, Duke Cancer Institute, Institutes and Centers 2013

Contact Information

C359 Lev Sci Res Ctr, Durham, NC 27708
Duke Box 3813, Durham, NC 27710
daniel.lew@duke.edu (919) 613-8627
Lew Lab

Background
Education, Training, & Certifications
- Ph.D., Rockefeller University 1990
Duke Appointment History
- Assistant Professor of Molecular Genetics and Microbiology, Molecular Genetics and Microbiology, Basic Science Departments 2002 - 2008
- Associate Professor of Pharmacology & Cancer Biology, Pharmacology & Cancer Biology, Basic Science Departments 2001 - 2006
- Assistant Professor of Genetics, School of Medicine, Duke University 1995 - 2002
- Assistant Professor of Pharmacology & Cancer Biology, Pharmacology & Cancer Biology, Basic Science Departments 1997 - 2001
- Assistant Professor of Molecular Cancer Biology, Pharmacology & Cancer Biology, Basic Science Departments 1994 - 1997
Recognition
Awards & Honors
Expertise
Subject Headings
Research
Selected Grants
- Studies of cell polarity, chemotropism, and cell-cycle control awarded by National Institutes of Health 2017 - 2022
- Training Program in Developmental and Stem Cell Biology awarded by National Institutes of Health 2001 - 2022
- Bioinformatics and Computational Biology Training Program awarded by National Institutes of Health 2005 - 2021
- Organization and Function of Cellular Structure awarded by National Institutes of Health 1975 - 2020
- Pharmacological Sciences Training Program awarded by National Institutes of Health 1975 - 2020
- Program to Support Student Development and Diversity in Duke Biosciences awarded by National Institutes of Health 2013 - 2019
- Mechanisms of cell fusion during mating in Saccharomyces cerevisiae awarded by Burroughs Wellcome Fund 2017 - 2018
- Spatiotemporal modeling of signal transduction in yeast awarded by University of North Carolina - Chapel Hill 2014 - 2018
- Gradient Tracking and Chemotropism awarded by National Institutes of Health 2013 - 2018
- Polarity Establishment in Yeast awarded by National Institutes of Health 2001 - 2017
- Cell Cycle Checkpoint that Monitors Morphogenesis awarded by National Institutes of Health 1995 - 2013
Publications & Artistic Works
Selected Publications
- Book Sections
  - Lew, DJ. "Cell Cycle." In Brenner's Encyclopedia of Genetics: Second Edition, 456-464. February 27, 2013. Full Text
- Conference Papers
  - Ghose, D, Lew, DJ, and Elston, TC. "Chemotropism in yeast." 2017. Link to Item
  - Daniels, CN, and Lew, DJ. "A novel role for Cdc42 effectors Gic1/2 in cell polarity." 2016. Link to Item
  - Kang, H, and Lew, DJ. "Elm1 regulates Hsl1/Hsl7 interaction in the morphogenesis checkpoint." 2015. Link to Item
  - Woods, B, Lai, H, Wu, C, Kuo, C, Zyla, T, and Lew, DJ. "Polarity establishment requires localized activation of Cdc42 by its GEF." 2015. Link to Item
  - Lai, H, Zyla, T, and Lew, DJ. "Regulation of pheromone-induced cell polarization by the polarisome proteins in yeast." December 2014. Link to Item
  - Woods, B, Wu, C, Zyla, T, Savage, N, and Lew, DJ. "A GDI-independent mechanism to concentrate the Rho-GTPase Cdc42 during polarity establishment." December 2014. Link to Item
  - Chen, H, Howell, AS, Kang, H, and Lew, DJ. "How does Cdc42p polarize actin cables?." 2011. Link to Item
  - Howell, AS, Irazoqui, JE, Theesfeld, CL, and Lew, DJ. "Opposing roles for actin in Cdc42p polarization." November 2004. Link to Item
  - Kozubowski, L, Larson, JR, Lew, DJ, and Tatchell, K. "Exploring the basis for the asymmetry of septin-dependent proteins at the mother-bud neck in Saccharomyces cerevisiae." November 2004. Link to Item
  - McNulty, JJ, and Lew, DJ. "Bud size doesnt matter." November 2004. Link to Item
  - Irazoqui, JE, and Lew, DJ. "The role of F-actin in Cdc42p polarization." July 2003. Link to Item
  - Harrison, JC, Bardes, EG, Zyla, TR, and Lew, DJ. "Stress-specific activation mechanisms for the "cell integrity" MAPK pathway in Saccharomyces cerevisiae." November 2002. Link to Item
  - Irazoqui, JE, and Lew, DJ. "Bem1p is required for Cdc42p polarization in the absence of bud site selection." November 2002. Link to Item
- Journal Articles
  - Chiou, J-G, Balasubramanian, MK, and Lew, DJ. "Cell Polarity in Yeast." Annual review of cell and developmental biology 33 (October 2017): 77-101. Full Text
  - Woods, B, and Lew, DJ. "Polarity establishment by Cdc42: Key roles for positive feedback and differential mobility." Small GTPases (March 28, 2017): 1-8. Full Text
  - Kang, H, and Lew, DJ. "How do cells know what shape they are?." Current genetics 63, no. 1 (February 2017): 75-77. (Review) Full Text
  - Woods, B, Lai, H, Wu, C-F, Zyla, TR, Savage, NS, and Lew, DJ. "Parallel Actin-Independent Recycling Pathways Polarize Cdc42 in Budding Yeast." Current biology : CB 26, no. 16 (August 2016): 2114-2126. Full Text Open Access Copy
  - Kang, H, Tsygankov, D, and Lew, DJ. "Sensing a bud in the yeast morphogenesis checkpoint: a role for Elm1." Molecular biology of the cell 27, no. 11 (June 2016): 1764-1775. Full Text
  - McClure, AW, Wu, C-F, Johnson, SA, and Lew, DJ. "Imaging Polarization in Budding Yeast." Methods in molecular biology (Clifton, N.J.) 1407 (January 2016): 13-23. Full Text Open Access Copy
  - Wu, C-F, Chiou, J-G, Minakova, M, Woods, B, Tsygankov, D, Zyla, TR, Savage, NS, Elston, TC, and Lew, DJ. "Role of competition between polarity sites in establishing a unique front." eLife 4 (November 2, 2015). Full Text
  - Wu, CF, Chiou, JG, Minakova, M, Woods, B, Tsygankov, D, Zyla, TR, Savage, NS, Elston, TC, and Lew, DJ. "Role of competition between polarity sites in establishing a unique front." eLife 4, no. NOVEMBER2015 (November 2, 2015). Full Text
  - McClure, AW, Minakova, M, Dyer, JM, Zyla, TR, Elston, TC, and Lew, DJ. "Role of Polarized G Protein Signaling in Tracking Pheromone Gradients." Developmental cell 35, no. 4 (November 2015): 471-482. Full Text
  - Ramirez, SA, Raghavachari, S, and Lew, DJ. "Dendritic spine geometry can localize GTPase signaling in neurons." Molecular biology of the cell 26, no. 22 (November 2015): 4171-4181. Full Text
  - Woods, B, Kuo, C-C, Wu, C-F, Zyla, TR, and Lew, DJ. "Polarity establishment requires localized activation of Cdc42." The Journal of cell biology 211, no. 1 (October 2015): 19-26. Full Text
  - McClure, AW, and Lew, DJ. "To avoid a mating mishap, yeast focus and communicate." The Journal of cell biology 208, no. 7 (March 2015): 867-868. (Review) Full Text
  - McClure, AW, and Lew, DJ. "Cell polarity: netrin calms an excitable system." Current biology : CB 24, no. 21 (November 3, 2014): R1050-R1052. Full Text
  - Kuo, C-C, Savage, NS, Chen, H, Wu, C-F, Zyla, TR, and Lew, DJ. "Inhibitory GEF phosphorylation provides negative feedback in the yeast polarity circuit." Current biology : CB 24, no. 7 (March 13, 2014): 753-759. Full Text Open Access Copy
  - Kuo, C-C, Savage, NS, Chen, H, Wu, C-F, Zyla, TR, and Lew, DJ. "Inhibitory GEF phosphorylation provides negative feedback in the yeast polarity circuit." Current Biology 24, no. 7 (2014): 753-759. Full Text
  - Wu, C-F, and Lew, DJ. "Beyond symmetry-breaking: competition and negative feedback in GTPase regulation." Trends Cell Biol 23, no. 10 (October 2013): 476-483. (Review) Full Text Link to Item
  - Atkins, BD, Yoshida, S, Saito, K, Wu, C-F, Lew, DJ, and Pellman, D. "Inhibition of Cdc42 during mitotic exit is required for cytokinesis." JOURNAL OF CELL BIOLOGY 202, no. 2 (July 22, 2013): 231-240. Full Text Link to Item
  - King, K, Kang, H, Jin, M, and Lew, DJ. "Feedback control of Swe1p degradation in the yeast morphogenesis checkpoint." Mol Biol Cell 24, no. 7 (April 2013): 914-922. Full Text Link to Item
  - Dyer, JM, Savage, NS, Jin, M, Zyla, TR, Elston, TC, and Lew, DJ. "Tracking shallow chemical gradients by actin-driven wandering of the polarization site." Curr Biol 23, no. 1 (January 7, 2013): 32-41. Full Text Link to Item
  - Dyer, JM, Savage, NS, Jin, M, Zyla, TR, Elston, TC, and Lew, DJ. "Tracking shallow chemical gradients by actin-driven wandering of the polarization site." Current Biology 23, no. 1 (2013): 32-41. Full Text
  - Wu, C-F, Savage, NS, Zyla, TR, and Lew, DJ. "The yeast guanine nucleotide dissociation inhibitor (GDI) enforces singularity by enhancing competition between polarity sites." MOLECULAR BIOLOGY OF THE CELL 24 (2013). Link to Item
  - Wu, C-F, Savage, NS, and Lew, DJ. "Interaction between bud-site selection and polarity-establishment machineries in budding yeast. (Published online)" Philos Trans R Soc Lond B Biol Sci 368, no. 1629 (2013): 20130006-. Full Text Link to Item
  - Wu, C-F, and Lew, DJ. "Beyond symmetry-breaking: Competition and negative feedback in GTPase regulation." Trends in Cell Biology 23, no. 10 (2013): 476-483. Full Text
  - Chen, H, Kuo, C-C, Kang, H, Howell, AS, Zyla, TR, Jin, M, and Lew, DJ. "Cdc42p regulation of the yeast formin Bni1p mediated by the effector Gic2p." Mol Biol Cell 23, no. 19 (October 2012): 3814-3826. Full Text Link to Item
  - Savage, NS, Layton, AT, and Lew, DJ. "Mechanistic mathematical model of polarity in yeast." Mol Biol Cell 23, no. 10 (May 2012): 1998-2013. Full Text Link to Item
  - Howell, AS, Jin, M, Wu, C-F, Zyla, TR, Elston, TC, and Lew, DJ. "Negative feedback enhances robustness in the yeast polarity establishment circuit." Cell 149, no. 2 (April 13, 2012): 322-333. Full Text Link to Item
  - Howell, AS, and Lew, DJ. "Morphogenesis and the cell cycle." Genetics 190, no. 1 (January 2012): 51-77. (Review) Full Text Link to Item
  - King, K, Jin, M, and Lew, D. "Roles of hsl1p and Hsl7p in Swe1p degradation: Beyond septin tethering." Eukaryotic Cell 11, no. 12 (2012): 1496-1502. Full Text
  - Wu, C-F, and Lew, DJ. "The yeast guanine nucleotide dissociation inhibitor (GDI) enforces singularity by enhancing competition between polarity sites." MOLECULAR BIOLOGY OF THE CELL 23 (2012). Link to Item
  - Johnson, JM, Jin, M, and Lew, DJ. "Symmetry breaking and the establishment of cell polarity in budding yeast." Curr Opin Genet Dev 21, no. 6 (December 2011): 740-746. (Review) Full Text Link to Item
  - Chen, H, Howell, AS, Robeson, A, and Lew, DJ. "Dynamics of septin ring and collar formation in Saccharomyces cerevisiae." Biol Chem 392, no. 8-9 (August 2011): 689-697. Full Text Link to Item
  - Layton, AT, Savage, NS, Howell, AS, Carroll, SY, Drubin, DG, and Lew, DJ. "Modeling vesicle traffic reveals unexpected consequences for Cdc42p-mediated polarity establishment." Curr Biol 21, no. 3 (February 8, 2011): 184-194. Full Text Link to Item
  - Howell, AS, Savage, NS, Johnson, SA, Bose, I, Wagner, AW, Zyla, TR, Nijhout, HF, Reed, MC, Goryachev, AB, and Lew, DJ. "Singularity in polarization: rewiring yeast cells to make two buds." Cell 139, no. 4 (November 13, 2009): 731-743. Full Text Link to Item
  - Crutchley, J, King, KM, Keaton, MA, Szkotnicki, L, Orlando, DA, Zyla, TR, Bardes, ESG, and Lew, DJ. "Molecular dissection of the checkpoint kinase Hsl1p." Mol Biol Cell 20, no. 7 (April 2009): 1926-1936. Full Text Link to Item
  - Kozubowski, L, Saito, K, Johnson, JM, Howell, AS, and Lew, DJ. "Response: GEF localization, not just activation, is needed for yeast polarity establishment." Current Biology 19, no. 5 (2009): R195-. Full Text
  - Lew, DJ, and Rout, MP. "Cell structure and dynamics." Current Opinion in Cell Biology 21, no. 1 (2009): 1-3. Full Text
  - Kozubowski, L, Saito, K, Johnson, JM, Howell, AS, Zyla, TR, and Lew, DJ. "Symmetry-breaking polarization driven by a Cdc42p GEF-PAK complex." Curr Biol 18, no. 22 (November 25, 2008): 1719-1726. Full Text Link to Item
  - Szkotnicki, L, Crutchley, JM, Zyla, TR, Bardes, ESG, and Lew, DJ. "The checkpoint kinase Hsl1p is activated by Elm1p-dependent phosphorylation." Mol Biol Cell 19, no. 11 (November 2008): 4675-4686. Full Text Link to Item
  - Keaton, MA, Szkotnicki, L, Marquitz, AR, Harrison, J, Zyla, TR, and Lew, DJ. "Nucleocytoplasmic trafficking of G2/M regulators in yeast." Mol Biol Cell 19, no. 9 (September 2008): 4006-4018. Full Text Link to Item
  - Lew, DJ, Burke, DJ, and Dutta, A. "The immortal strand hypothesis: how could it work?." Cell 133, no. 1 (April 4, 2008): 21-23. (Letter) Full Text Link to Item
  - York, JD, and Lew, DJ. "IP7 guards the CDK gate." Nat Chem Biol 4, no. 1 (January 2008): 16-17. Full Text Link to Item
  - Tong, Z, Gao, X-D, Howell, AS, Bose, I, Lew, DJ, and Bi, E. "Adjacent positioning of cellular structures enabled by a Cdc42 GTPase-activating protein-mediated zone of inhibition." J Cell Biol 179, no. 7 (December 31, 2007): 1375-1384. Full Text Link to Item
  - Keaton, MA, Bardes, ESG, Marquitz, AR, Freel, CD, Zyla, TR, Rudolph, J, and Lew, DJ. "Differential susceptibility of yeast S and M phase CDK complexes to inhibitory tyrosine phosphorylation." Curr Biol 17, no. 14 (July 17, 2007): 1181-1189. Full Text Link to Item
  - Haase, SB, and Lew, DJ. "Microtubule organization: cell shape is destiny." Curr Biol 17, no. 7 (April 3, 2007): R249-R251. Full Text Link to Item
  - Keaton, MA, and Lew, DJ. "Eavesdropping on the cytoskeleton: progress and controversy in the yeast morphogenesis checkpoint." Curr Opin Microbiol 9, no. 6 (December 2006): 540-546. (Review) Full Text Link to Item
  - Lew, DJ. "Yeast polarity: negative feedback shifts the focus." Curr Biol 15, no. 24 (December 20, 2005): R994-R996. (Review) Full Text Link to Item
  - McNulty, JJ, and Lew, DJ. "Swe1p responds to cytoskeletal perturbation, not bud size, in S. cerevisiae." Curr Biol 15, no. 24 (December 20, 2005): 2190-2198. Full Text Link to Item
  - Gladfelter, AS, Kozubowski, L, Zyla, TR, and Lew, DJ. "Interplay between septin organization, cell cycle and cell shape in yeast." J Cell Sci 118, no. Pt 8 (April 15, 2005): 1617-1628. Full Text Link to Item
  - Irazoqui, JE, Howell, AS, Theesfeld, CL, and Lew, DJ. "Opposing roles for actin in Cdc42p polarization." Mol Biol Cell 16, no. 3 (March 2005): 1296-1304. Full Text Link to Item
  - Gladfelter, AS, Zyla, TR, and Lew, DJ. "Genetic interactions among regulators of septin organization." Eukaryot Cell 3, no. 4 (August 2004): 847-854. Full Text Link to Item
  - Irazoqui, JE, Gladfelter, AS, and Lew, DJ. "Cdc42p, GTP hydrolysis, and the cell's sense of direction." Cell Cycle 3, no. 7 (July 2004): 861-864. (Review) Link to Item
  - Irazoqui, JE, and Lew, DJ. "Polarity establishment in yeast." J Cell Sci 117, no. Pt 11 (May 1, 2004): 2169-2171. (Review) Full Text Link to Item
  - Harrison, JC, Zyla, TR, Bardes, ESG, and Lew, DJ. "Stress-specific activation mechanisms for the "cell integrity" MAPK pathway." J Biol Chem 279, no. 4 (January 23, 2004): 2616-2622. Full Text Link to Item
  - Irazoqui, JE, Gladfelter, AS, and Lew, DJ. "Scaffold-mediated symmetry breaking by Cdc42p." Nat Cell Biol 5, no. 12 (December 2003): 1062-1070. Full Text Link to Item
  - Lew, DJ. "The morphogenesis checkpoint: how yeast cells watch their figures." Curr Opin Cell Biol 15, no. 6 (December 2003): 648-653. (Review) Link to Item
  - Theesfeld, CL, Zyla, TR, Bardes, EGS, and Lew, DJ. "A monitor for bud emergence in the yeast morphogenesis checkpoint." Mol Biol Cell 14, no. 8 (August 2003): 3280-3291. Full Text Link to Item
  - Lew, DJ, and Burke, DJ. "The spindle assembly and spindle position checkpoints." Annu Rev Genet 37 (2003): 251-282. (Review) Full Text Link to Item
  - McMillan, JN, Theesfeld, CL, Harrison, JC, Bardes, ESG, and Lew, DJ. "Determinants of Swe1p degradation in Saccharomyces cerevisiae." Mol Biol Cell 13, no. 10 (October 2002): 3560-3575. Full Text Link to Item
  - Marquitz, AR, Harrison, JC, Bose, I, Zyla, TR, McMillan, JN, and Lew, DJ. "The Rho-GAP Bem2p plays a GAP-independent role in the morphogenesis checkpoint." EMBO J 21, no. 15 (August 1, 2002): 4012-4025. Full Text Link to Item
  - Lew, DJ. "Formin' actin filament bundles." Nat Cell Biol 4, no. 2 (February 2002): E29-E30. Full Text Link to Item
  - Gladfelter, AS, Bose, I, Zyla, TR, Bardes, ESG, and Lew, DJ. "Septin ring assembly involves cycles of GTP loading and hydrolysis by Cdc42p." J Cell Biol 156, no. 2 (January 21, 2002): 315-326. Full Text Link to Item
  - Gladfelter, AS, Pringle, JR, and Lew, DJ. "The septin cortex at the yeast mother-bud neck." Curr Opin Microbiol 4, no. 6 (December 2001): 681-689. (Review) Link to Item
  - Adamo, JE, Moskow, JJ, Gladfelter, AS, Viterbo, D, Lew, DJ, and Brennwald, PJ. "Yeast Cdc42 functions at a late step in exocytosis, specifically during polarized growth of the emerging bud." J Cell Biol 155, no. 4 (November 12, 2001): 581-592. Full Text Link to Item
  - Marquitz, AR, Harrison, JC, Theesfeld, CL, Bardes, ESG, and Lew, DJ. "The Rho GAP Bem2p plays a role in the morphogenesis checkpoint of Saccharomyces cerevisiae." MOLECULAR BIOLOGY OF THE CELL 12 (November 2001): 408A-408A. Link to Item
  - Theesfeld, CL, and Lew, DJ. "Septin-dependent monitoring of local cell shape in the yeast morphogenesis checkpoint." MOLECULAR BIOLOGY OF THE CELL 12 (November 2001): 142A-143A. Link to Item
  - Theesfeld, CL, Bardes, E, and Lew, DJ. "Hsl1 as a sensor of cell shape." YEAST 18 (August 2001): S142-S142. Link to Item
  - Gladfelter, AS, Moskow, JJ, Zyla, TR, and Lew, DJ. "Isolation and characterization of effector-loop mutants of CDC42 in yeast." Mol Biol Cell 12, no. 5 (May 2001): 1239-1255. Link to Item
  - Harrison, JC, Bardes, ES, Ohya, Y, and Lew, DJ. "A role for the Pkc1p/Mpk1p kinase cascade in the morphogenesis checkpoint." Nat Cell Biol 3, no. 4 (April 2001): 417-420. Full Text Link to Item
  - Bose, I, Irazoqui, JE, Moskow, JJ, Bardes, ES, Zyla, TR, and Lew, DJ. "Assembly of scaffold-mediated complexes containing Cdc42p, the exchange factor Cdc24p, and the effector Cla4p required for cell cycle-regulated phosphorylation of Cdc24p." J Biol Chem 276, no. 10 (March 9, 2001): 7176-7186. Full Text Link to Item
  - Adamo, JE, Moskow, JJ, Gladfelter, AS, Viterbo, D, Lew, DJ, and Brennwald, PJ. "Yeast Cdc42 functions at a late step in exocytosis, specifically during polarized growth of the emerging bud." Journal of Cell Biology 155, no. 3 (2001): 581-592. Full Text
  - Yeh, E, Yang, C, Chin, E, Maddox, P, Salmon, ED, Lew, DJ, and Bloom, K. "Dynamic positioning of mitotic spindles in yeast: role of microtubule motors and cortical determinants." Mol Biol Cell 11, no. 11 (November 2000): 3949-3961. Link to Item
  - Moskow, JJ, Gladfelter, AS, Lamson, RE, Pryciak, PM, and Lew, DJ. "Role of Cdc42p in pheromone-stimulated signal transduction in Saccharomyces cerevisiae." Mol Cell Biol 20, no. 20 (October 2000): 7559-7571. Link to Item
  - Longtine, MS, Theesfeld, CL, McMillan, JN, Weaver, E, Pringle, JR, and Lew, DJ. "Septin-dependent assembly of a cell cycle-regulatory module in Saccharomyces cerevisiae." Mol Cell Biol 20, no. 11 (June 2000): 4049-4061. Link to Item
  - Lew, DJ. "Cell-cycle checkpoints that ensure coordination between nuclear and cytoplasmic events in Saccharomyces cerevisiae." Curr Opin Genet Dev 10, no. 1 (February 2000): 47-53. (Review) Link to Item
  - Kim, JM, Chang, KH, Choi, YH, Song, YG, Kang, SM, Yoon, TY, Choi, JM, Park, SY, and Lew, DJ. "Seroprevalence of HTLV-I in Cheju Island, a Korean island adjacent to the endemic area of Japan." J Acquir Immune Defic Syndr 22, no. 4 (December 1, 1999): 409-412. (Letter) Link to Item
  - Gladfelter, AS, Moskow, JJ, and Lew, DJ. "Analysis of cdc42effector loop mutants in budding yeast." MOLECULAR BIOLOGY OF THE CELL 10 (November 1999): 54A-54A. Link to Item
  - Lew, DJ. "Cell cycle and checkpoint control of Swe1p localization and degradation." MOLECULAR BIOLOGY OF THE CELL 10 (November 1999): 3A-3A. Link to Item
  - McMillan, JN, Longtine, MS, Sia, RA, Theesfeld, CL, Bardes, ES, Pringle, JR, and Lew, DJ. "The morphogenesis checkpoint in Saccharomyces cerevisiae: cell cycle control of Swe1p degradation by Hsl1p and Hsl7p." Mol Cell Biol 19, no. 10 (October 1999): 6929-6939. Link to Item
  - Theesfeld, CL, Irazoqui, JE, Bloom, K, and Lew, DJ. "The role of actin in spindle orientation changes during the Saccharomyces cerevisiae cell cycle." J Cell Biol 146, no. 5 (September 6, 1999): 1019-1032. Link to Item
  - McMillan, JN, Sia, RA, Bardes, ES, and Lew, DJ. "Phosphorylation-independent inhibition of Cdc28p by the tyrosine kinase Swe1p in the morphogenesis checkpoint." Mol Cell Biol 19, no. 9 (September 1999): 5981-5990. Link to Item
  - Sia, RA, Bardes, ES, and Lew, DJ. "Control of Swe1p degradation by the morphogenesis checkpoint." EMBO J 17, no. 22 (November 16, 1998): 6678-6688. Full Text Link to Item
  - McMillan, JN, Sia, RA, and Lew, DJ. "A morphogenesis checkpoint monitors the actin cytoskeleton in yeast." J Cell Biol 142, no. 6 (September 21, 1998): 1487-1499. Link to Item
  - Bi, E, Maddox, P, Lew, DJ, Salmon, ED, McMillan, JN, Yeh, E, and Pringle, JR. "Involvement of an actomyosin contractile ring in Saccharomyces cerevisiae cytokinesis." J Cell Biol 142, no. 5 (September 7, 1998): 1301-1312. Link to Item
  - Kaiser, P, Sia, RA, Bardes, EG, Lew, DJ, and Reed, SI. "Cdc34 and the F-box protein Met30 are required for degradation of the Cdk-inhibitory kinase Swe1." Genes Dev 12, no. 16 (August 15, 1998): 2587-2597. Link to Item
  - Haase, SB, and Lew, DJ. "Flow cytometric analysis of DNA content in budding yeast." Methods Enzymol 283 (1997): 322-332. Link to Item
  - Lew, DJ, and Kornbluth, S. "Regulatory roles of cyclin dependent kinase phosphorylation in cell cycle control." Curr Opin Cell Biol 8, no. 6 (December 1996): 795-804. (Review) Link to Item
  - McMillan, JN, and Lew, DJ. "Genetic screen to uncover proteins involved in the morphogenesis cell cycle checkpoint in budding yeast." MOLECULAR BIOLOGY OF THE CELL 7 (December 1996): 2077-2077. Link to Item
  - Sia, RA, Herald, HA, and Lew, DJ. "Cdc28 tyrosine phosphorylation and the morphogenesis checkpoint in budding yeast." Mol Biol Cell 7, no. 11 (November 1996): 1657-1666. Link to Item
  - Lew, DJ, and Reed, SI. "A cell cycle checkpoint monitors cell morphogenesis in budding yeast." J Cell Biol 129, no. 3 (May 1995): 739-749. Link to Item
  - Parks, B, Spielman, S, Orenstein, J, Nemeth, DT, Ludwig, F, Clarke, J, Merchant, P, and Lew, DJ. "Phase-sensitive measurements of vortex dynamics in the terahertz domain." Phys Rev Lett 74, no. 16 (April 17, 1995): 3265-3268. Full Text Link to Item
  - Lew, DJ, and Reed, SI. "Cell cycle control of morphogenesis in budding yeast." Curr Opin Genet Dev 5, no. 1 (February 1995): 17-23. (Review) Link to Item
  - Spielman, S, Parks, B, Orenstein, J, Nemeth, DT, Ludwig, F, Clarke, J, Merchant, P, and Lew, DJ. "Observation of the quasiparticle Hall effect in superconducting YBa2Cu3O7- delta." Phys Rev Lett 73, no. 11 (September 12, 1994): 1537-1540. Full Text Link to Item
  - Stueland, CS, Lew, DJ, Cismowski, MJ, and Reed, SI. "Full activation of p34CDC28 histone H1 kinase activity is unable to promote entry into mitosis in checkpoint-arrested cells of the yeast Saccharomyces cerevisiae." Mol Cell Biol 13, no. 6 (June 1993): 3744-3755. Link to Item
  - Lew, DJ, and Reed, SI. "Morphogenesis in the yeast cell cycle: regulation by Cdc28 and cyclins." J Cell Biol 120, no. 6 (March 1993): 1305-1320. Link to Item
  - Lew, DJ, Marini, NJ, and Reed, SI. "A suppressor of cln3 for size control." Cell 72, no. 4 (1993): 488-489. Full Text
  - Richardson, H, Lew, DJ, Henze, M, Sugimoto, K, and Reed, SI. "Cyclin-B homologs in Saccharomyces cerevisiae function in S phase and in G2." Genes Dev 6, no. 11 (November 1992): 2021-2034. Link to Item
  - Lew, DJ, and I Reed, S. "A proliferation of cyclins." Trends Cell Biol 2, no. 3 (March 1992): 77-81. Link to Item
  - Lew, DJ, Marini, NJ, and Reed, SI. "Different G1 cyclins control the timing of cell cycle commitment in mother and daughter cells of the budding yeast S. cerevisiae." Cell 69, no. 2 (1992): 317-327. Full Text
  - Lu, XP, Koch, KS, Lew, DJ, Dulic, V, Pines, J, Reed, SI, Hunter, T, and Leffert, HL. "Induction of cyclin mRNA and cyclin-associated histone H1 kinase during liver regeneration." Journal of Biological Chemistry 267, no. 5 (1992): 2841-2844.
  - Reed, SI, Dulic, V, Lew, DJ, Richardson, HE, and Wittenberg, C. "G1 control in yeast and animal cells." Ciba Found Symp 170 (1992): 7-15. (Review) Link to Item
  - Decker, T, Lew, DJ, and Darnell, JE. "Two distinct alpha-interferon-dependent signal transduction pathways may contribute to activation of transcription of the guanylate-binding protein gene." Mol Cell Biol 11, no. 10 (October 1991): 5147-5153. Link to Item
  - Lew, DJ, Dulić, V, and Reed, SI. "Isolation of three novel human cyclins by rescue of G1 cyclin (Cln) function in yeast." Cell 66, no. 6 (September 20, 1991): 1197-1206. Link to Item
  - Lew, DJ, and Simon, SM. "Characterization of constitutive exocytosis in the yeast Saccharomyces cerevisiae." J Membr Biol 123, no. 3 (September 1991): 261-268. Link to Item
  - Ghiara, JB, Richardson, HE, Sugimoto, K, Henze, M, Lew, DJ, Wittenberg, C, and Reed, SI. "A cyclin B homolog in S. cerevisiae: chronic activation of the Cdc28 protein kinase by cyclin prevents exit from mitosis." Cell 65, no. 1 (April 5, 1991): 163-174. Link to Item
  - Decker, T, Lew, DJ, Mirkovitch, J, and Darnell, JE. "Cytoplasmic activation of GAF, an IFN-gamma-regulated DNA-binding factor." EMBO J 10, no. 4 (April 1991): 927-932. Link to Item
  - Lew, DJ, Decker, T, Strehlow, I, and Darnell, JE. "Overlapping elements in the guanylate-binding protein gene promoter mediate transcriptional induction by alpha and gamma interferons." Mol Cell Biol 11, no. 1 (January 1991): 182-191. Link to Item
  - Reed, SI, Wittenberg, C, Lew, DJ, Dulic, V, and Henze, M. "G1 control in yeast and animal cells." Cold Spring Harb Symp Quant Biol 56 (1991): 61-67. Link to Item
  - Reed, SI, Wittenberg, C, Lew, DJ, Dulic, V, and Henze, M. "G₁ control in yeast and animal cells." Cold Spring Harbor Symposia on Quantitative Biology 56 (1991): 61-68.
  - Levy, DE, Lew, DJ, Decker, T, Kessler, DS, and Darnell, JE. "Synergistic interaction between interferon-alpha and interferon-gamma through induced synthesis of one subunit of the transcription factor ISGF3." EMBO J 9, no. 4 (April 1990): 1105-1111. Link to Item
  - Lew, DJ, Decker, T, and Darnell, JE. "Alpha interferon and gamma interferon stimulate transcription of a single gene through different signal transduction pathways." Mol Cell Biol 9, no. 12 (December 1989): 5404-5411. Link to Item
  - Decker, T, Lew, DJ, Cheng, YS, Levy, DE, and Darnell, JE. "Interactions of alpha- and gamma-interferon in the transcriptional regulation of the gene encoding a guanylate-binding protein." EMBO J 8, no. 7 (July 1989): 2009-2014. Link to Item
  - Bookchin, RM, Lew, DJ, Balazs, T, Ueda, Y, and Lew, VL. "Dehydration and delayed proton equilibria of red blood cells suspended in isosmotic phosphate buffers. Implications for studies of sickled cells." J Lab Clin Med 104, no. 6 (December 1984): 855-866. Link to Item
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