Daniel Julio Lew | Scholars@Duke

Daniel Julio Lew
James B. Duke Distinguished Professor of Pharmacology and Cancer Biology

Our research interests focus on the control of cell polarity. Cell polarity is a nearly universal feature of eukaryotic cells. A polarized cell usually has a single, clear axis of asymmetry: a “front” and a “back”. In the past several years it has become apparent that the highly conserved Rho-family GTPase Cdc42, first discovered in yeast, is a component of a master pathway, employed time and again to promote polarity in different contexts.

Most cells know which way to polarize. Concentration gradients of attractants, repellents, nutrients, or pheromones reveal the optimal directions for successful attack, escape, feeding, or mating. However, cells can and do polarize even when deprived of directional cues, choosing a random axis and committing to it as if they knew where they were going. This process, called "symmetry breaking", reflects the presence of a core internal polarity program. Our work has uncovered the biochemical basis for this core program, which uses positive feedback loops to reinforce inequalities in the local concentrations of polarity factors, so that stochastic fluctuations are amplified into a single dominating asymmetry.

We use the tractable budding yeast as a model system. Because the genes and processes we study are highly conserved, we anticipate that learning the answers to fundamental questions in yeast will be relevant and informative for a wide range of organisms. Our work combines molecular genetics, cell biology, and mathematical modeling, and addresses questions including:

Why is there one and only one “front”?
How is polarity turned on and off?
How does Cdc42 organize the cytoskeleton?
How is polarity guided by pheromone gradients?

Current Appointments & Affiliations

James B. Duke Distinguished Professor of Pharmacology and Cancer Biology, Pharmacology & Cancer Biology, Basic Science Departments 2011
Professor of Pharmacology and Cancer Biology, Pharmacology & Cancer Biology, Basic Science Departments 2006
Professor in Molecular Genetics and Microbiology, Molecular Genetics and Microbiology, Basic Science Departments 2008
Professor of Cell Biology, Cell Biology, Basic Science Departments 2009
Member of the Duke Cancer Institute, Duke Cancer Institute, Institutes and Centers 2013

Contact Information

C359 Lev Sci Res Ctr, Durham, NC 27708
Duke Box 3813, Durham, NC 27710
daniel.lew@duke.edu (919) 613-8627
Lew Lab

Background
Education, Training, & Certifications
- Ph.D., Rockefeller University 1990
Duke Appointment History
- Assistant Professor of Molecular Genetics and Microbiology, Molecular Genetics and Microbiology, Basic Science Departments 2002 - 2008
- Associate Professor of Pharmacology & Cancer Biology, Pharmacology & Cancer Biology, Basic Science Departments 2001 - 2006
- Assistant Professor of Genetics, School of Medicine, Duke University 1995 - 2002
- Assistant Professor of Pharmacology & Cancer Biology, Pharmacology & Cancer Biology, Basic Science Departments 1997 - 2001
- Assistant Professor of Molecular Cancer Biology, Pharmacology & Cancer Biology, Basic Science Departments 1994 - 1997
Recognition
Awards & Honors
Expertise
Subject Headings
Research
Selected Grants
- Studies of cell polarity, chemotropism, and cell-cycle control awarded by National Institutes of Health 2017 - 2022
- Studies of cell polarity, chemotropism, and cell-cycle control awarded by National Institutes of Health 2017 - 2022
- Training Program in Developmental and Stem Cell Biology awarded by National Institutes of Health 2001 - 2022
- Bioinformatics and Computational Biology Training Program awarded by National Institutes of Health 2005 - 2020
- Genetics Training Grant awarded by National Institutes of Health 1979 - 2020
- Genetics Training Grant awarded by National Institutes of Health 1979 - 2020
- Organization and Function of Cellular Structure awarded by National Institutes of Health 1975 - 2020
- Organization and Function of Cellular Structure awarded by National Institutes of Health 1975 - 2020
- Pharmacological Sciences Training Program awarded by National Institutes of Health 1975 - 2020
- Program to Support Student Development and Diversity in Duke Biosciences awarded by National Institutes of Health 2013 - 2019
- Mechanisms of cell fusion during mating in Saccharomyces cerevisiae awarded by Burroughs Wellcome Fund 2017 - 2018
- Spatiotemporal modeling of signal transduction in yeast awarded by University of North Carolina - Chapel Hill 2014 - 2018
- Gradient Tracking and Chemotropism awarded by National Institutes of Health 2013 - 2018
- Polarity Establishment in Yeast awarded by National Institutes of Health 2001 - 2017
- Cell Cycle Checkpoint that Monitors Morphogenesis awarded by National Institutes of Health 1995 - 2013
Publications & Artistic Works
Selected Publications
- Academic Articles
  - Mitchison-Field, Lorna M. Y., José M. Vargas-Muñiz, Benjamin M. Stormo, Ellysa J. D. Vogt, Sarah Van Dierdonck, James F. Pelletier, Christoph Ehrlich, Daniel J. Lew, Christine M. Field, and Amy S. Gladfelter. “Unconventional Cell Division Cycles from Marine-Derived Yeasts..” Curr Biol 29, no. 20 (October 21, 2019): 3439-3456.e5. https://doi.org/10.1016/j.cub.2019.08.050.
    Full Text Link to Item
  - Henderson, Nicholas T., Michael Pablo, Debraj Ghose, Manuella R. Clark-Cotton, Trevin R. Zyla, James Nolen, Timothy C. Elston, and Daniel J. Lew. “Ratiometric GPCR signaling enables directional sensing in yeast..” Plos Biol 17, no. 10 (October 2019). https://doi.org/10.1371/journal.pbio.3000484.
    Full Text Link to Item
  - Woods, Benjamin, and Daniel J. Lew. “Polarity establishment by Cdc42: Key roles for positive feedback and differential mobility..” Small Gtpases 10, no. 2 (March 2019): 130–37. https://doi.org/10.1080/21541248.2016.1275370.
    Full Text Link to Item
  - Moran, Kyle D., Hui Kang, Ana V. Araujo, Trevin R. Zyla, Koji Saito, Denis Tsygankov, and Daniel J. Lew. “Cell-cycle control of cell polarity in yeast..” J Cell Biol 218, no. 1 (January 7, 2019): 171–89. https://doi.org/10.1083/jcb.201806196.
    Full Text Link to Item
  - McClure, Allison W., Katherine C. Jacobs, Trevin R. Zyla, and Daniel J. Lew. “Mating in wild yeast: delayed interest in sex after spore germination..” Mol Biol Cell, October 24, 2018. https://doi.org/10.1091/mbc.E18-08-0528.
    Full Text Link to Item
  - Lai, Helen, Jian-Geng Chiou, Anastasia Zhurikhina, Trevin R. Zyla, Denis Tsygankov, and Daniel J. Lew. “Temporal regulation of morphogenetic events in Saccharomyces cerevisiae..” Mol Biol Cell 29, no. 17 (August 15, 2018): 2069–83. https://doi.org/10.1091/mbc.E18-03-0188.
    Full Text Link to Item
  - Chiou, Jian-Geng, Samuel A. Ramirez, Timothy C. Elston, Thomas P. Witelski, David G. Schaeffer, and Daniel J. Lew. “Principles that govern competition or co-existence in Rho-GTPase driven polarization..” Plos Comput Biol 14, no. 4 (April 2018). https://doi.org/10.1371/journal.pcbi.1006095.
    Full Text Open Access Copy Link to Item
  - Daniels, Christine N., Trevin R. Zyla, and Daniel J. Lew. “A role for Gic1 and Gic2 in Cdc42 polarization at elevated temperature..” Plos One 13, no. 12 (2018). https://doi.org/10.1371/journal.pone.0200863.
    Full Text Link to Item
  - Chiou, Jian-Geng, Mohan K. Balasubramanian, and Daniel J. Lew. “Cell Polarity in Yeast..” Annu Rev Cell Dev Biol 33 (October 6, 2017): 77–101. https://doi.org/10.1146/annurev-cellbio-100616-060856.
    Full Text Link to Item
  - Kang, Hui, and Daniel J. Lew. “How do cells know what shape they are?.” Curr Genet 63, no. 1 (February 2017): 75–77. https://doi.org/10.1007/s00294-016-0623-1.
    Full Text Link to Item
  - Woods, Benjamin, Helen Lai, Chi-Fang Wu, Trevin R. Zyla, Natasha S. Savage, and Daniel J. Lew. “Parallel Actin-Independent Recycling Pathways Polarize Cdc42 in Budding Yeast..” Curr Biol 26, no. 16 (August 22, 2016): 2114–26. https://doi.org/10.1016/j.cub.2016.06.047.
    Full Text Open Access Copy Link to Item
  - Kang, Hui, Denis Tsygankov, and Daniel J. Lew. “Sensing a bud in the yeast morphogenesis checkpoint: a role for Elm1..” Mol Biol Cell 27, no. 11 (June 1, 2016): 1764–75. https://doi.org/10.1091/mbc.E16-01-0014.
    Full Text Link to Item
  - McClure, Allison W., Chi-Fang Wu, Sam A. Johnson, and Daniel J. Lew. “Imaging Polarization in Budding Yeast..” Methods Mol Biol 1407 (2016): 13–23. https://doi.org/10.1007/978-1-4939-3480-5_2.
    Full Text Open Access Copy Link to Item
  - McClure, Allison W., Maria Minakova, Jayme M. Dyer, Trevin R. Zyla, Timothy C. Elston, and Daniel J. Lew. “Role of Polarized G Protein Signaling in Tracking Pheromone Gradients..” Dev Cell 35, no. 4 (November 23, 2015): 471–82. https://doi.org/10.1016/j.devcel.2015.10.024.
    Full Text Link to Item
  - Ramirez, Samuel A., Sridhar Raghavachari, and Daniel J. Lew. “Dendritic spine geometry can localize GTPase signaling in neurons..” Mol Biol Cell 26, no. 22 (November 5, 2015): 4171–81. https://doi.org/10.1091/mbc.E15-06-0405.
    Full Text Link to Item
  - Wu, C. F., J. G. Chiou, M. Minakova, B. Woods, D. Tsygankov, T. R. Zyla, N. S. Savage, T. C. Elston, and D. J. Lew. “Role of competition between polarity sites in establishing a unique front.” Elife 4, no. NOVEMBER2015 (November 2, 2015). https://doi.org/10.7554/eLife.11611.001.
    Full Text
  - Wu, Chi-Fang, Jian-Geng Chiou, Maria Minakova, Benjamin Woods, Denis Tsygankov, Trevin R. Zyla, Natasha S. Savage, Timothy C. Elston, and Daniel J. Lew. “Role of competition between polarity sites in establishing a unique front..” Elife 4 (November 2, 2015). https://doi.org/10.7554/eLife.11611.
    Full Text Link to Item
  - Woods, Benjamin, Chun-Chen Kuo, Chi-Fang Wu, Trevin R. Zyla, and Daniel J. Lew. “Polarity establishment requires localized activation of Cdc42..” J Cell Biol 211, no. 1 (October 12, 2015): 19–26. https://doi.org/10.1083/jcb.201506108.
    Full Text Link to Item
  - McClure, Allison W., and Daniel J. Lew. “To avoid a mating mishap, yeast focus and communicate..” J Cell Biol 208, no. 7 (March 30, 2015): 867–68. https://doi.org/10.1083/jcb.201502095.
    Full Text Link to Item
  - McClure, Allison W., and Daniel J. Lew. “Cell polarity: netrin calms an excitable system..” Curr Biol 24, no. 21 (November 3, 2014): R1050–52. https://doi.org/10.1016/j.cub.2014.09.042.
    Full Text Link to Item
  - Kuo, Chun-Chen, Natasha S. Savage, Hsin Chen, Chi-Fang Wu, Trevin R. Zyla, and Daniel J. Lew. “Inhibitory GEF phosphorylation provides negative feedback in the yeast polarity circuit..” Curr Biol 24, no. 7 (March 31, 2014): 753–59. https://doi.org/10.1016/j.cub.2014.02.024.
    Full Text Open Access Copy Link to Item
  - Wu, Chi-Fang, and Daniel J. Lew. “Beyond symmetry-breaking: competition and negative feedback in GTPase regulation..” Trends Cell Biol 23, no. 10 (October 2013): 476–83. https://doi.org/10.1016/j.tcb.2013.05.003.
    Full Text Link to Item
  - Atkins, Benjamin D., Satoshi Yoshida, Koji Saito, Chi-Fang Wu, Daniel J. Lew, and David Pellman. “Inhibition of Cdc42 during mitotic exit is required for cytokinesis..” J Cell Biol 202, no. 2 (July 22, 2013): 231–40. https://doi.org/10.1083/jcb.201301090.
    Full Text Link to Item
  - King, Kindra, Hui Kang, Michelle Jin, and Daniel J. Lew. “Feedback control of Swe1p degradation in the yeast morphogenesis checkpoint..” Mol Biol Cell 24, no. 7 (April 2013): 914–22. https://doi.org/10.1091/mbc.E12-11-0812.
    Full Text Link to Item
  - Dyer, Jayme M., Natasha S. Savage, Meng Jin, Trevin R. Zyla, Timothy C. Elston, and Daniel J. Lew. “Tracking shallow chemical gradients by actin-driven wandering of the polarization site..” Curr Biol 23, no. 1 (January 7, 2013): 32–41. https://doi.org/10.1016/j.cub.2012.11.014.
    Full Text Link to Item
  - Wu, C. -. F., N. S. Savage, T. R. Zyla, and D. J. Lew. “The yeast guanine nucleotide dissociation inhibitor (GDI) enforces singularity by enhancing competition between polarity sites.” Molecular Biology of the Cell 24 (January 1, 2013).
    Link to Item
  - Wu, Chi-Fang, Natasha S. Savage, and Daniel J. Lew. “Interaction between bud-site selection and polarity-establishment machineries in budding yeast..” Philos Trans R Soc Lond B Biol Sci 368, no. 1629 (2013). https://doi.org/10.1098/rstb.2013.0006.
    Full Text Link to Item
  - King, Kindra, Michelle Jin, and Daniel Lew. “Roles of Hsl1p and Hsl7p in Swe1p degradation: beyond septin tethering..” Eukaryot Cell 11, no. 12 (December 2012): 1496–1502. https://doi.org/10.1128/EC.00196-12.
    Full Text Link to Item
  - Chen, Hsin, Chun-Chen Kuo, Hui Kang, Audrey S. Howell, Trevin R. Zyla, Michelle Jin, and Daniel J. Lew. “Cdc42p regulation of the yeast formin Bni1p mediated by the effector Gic2p..” Mol Biol Cell 23, no. 19 (October 2012): 3814–26. https://doi.org/10.1091/mbc.E12-05-0400.
    Full Text Link to Item
  - Savage, Natasha S., Anita T. Layton, and Daniel J. Lew. “Mechanistic mathematical model of polarity in yeast..” Mol Biol Cell 23, no. 10 (May 2012): 1998–2013. https://doi.org/10.1091/mbc.E11-10-0837.
    Full Text Link to Item
  - Howell, Audrey S., Meng Jin, Chi-Fang Wu, Trevin R. Zyla, Timothy C. Elston, and Daniel J. Lew. “Negative feedback enhances robustness in the yeast polarity establishment circuit..” Cell 149, no. 2 (April 13, 2012): 322–33. https://doi.org/10.1016/j.cell.2012.03.012.
    Full Text Link to Item
  - Howell, Audrey S., and Daniel J. Lew. “Morphogenesis and the cell cycle..” Genetics 190, no. 1 (January 2012): 51–77. https://doi.org/10.1534/genetics.111.128314.
    Full Text Link to Item
  - Wu, C. -. F., and D. J. Lew. “The yeast guanine nucleotide dissociation inhibitor (GDI) enforces singularity by enhancing competition between polarity sites..” Molecular Biology of the Cell 23 (January 1, 2012).
    Link to Item
  - Johnson, Jayme M., Meng Jin, and Daniel J. Lew. “Symmetry breaking and the establishment of cell polarity in budding yeast..” Curr Opin Genet Dev 21, no. 6 (December 2011): 740–46. https://doi.org/10.1016/j.gde.2011.09.007.
    Full Text Link to Item
  - Chen, Hsin, Audrey S. Howell, Alex Robeson, and Daniel J. Lew. “Dynamics of septin ring and collar formation in Saccharomyces cerevisiae..” Biol Chem 392, no. 8–9 (August 2011): 689–97. https://doi.org/10.1515/BC.2011.075.
    Full Text Link to Item
  - Layton, Anita T., Natasha S. Savage, Audrey S. Howell, Susheela Y. Carroll, David G. Drubin, and Daniel J. Lew. “Modeling vesicle traffic reveals unexpected consequences for Cdc42p-mediated polarity establishment..” Curr Biol 21, no. 3 (February 8, 2011): 184–94. https://doi.org/10.1016/j.cub.2011.01.012.
    Full Text Link to Item
  - Howell, Audrey S., Natasha S. Savage, Sam A. Johnson, Indrani Bose, Allison W. Wagner, Trevin R. Zyla, H Frederik Nijhout, Michael C. Reed, Andrew B. Goryachev, and Daniel J. Lew. “Singularity in polarization: rewiring yeast cells to make two buds..” Cell 139, no. 4 (November 13, 2009): 731–43. https://doi.org/10.1016/j.cell.2009.10.024.
    Full Text Link to Item
  - Crutchley, John, Kindra M. King, Mignon A. Keaton, Lee Szkotnicki, David A. Orlando, Trevin R. Zyla, Elaine S. G. Bardes, and Daniel J. Lew. “Molecular dissection of the checkpoint kinase Hsl1p..” Mol Biol Cell 20, no. 7 (April 2009): 1926–36. https://doi.org/10.1091/mbc.e08-08-0848.
    Full Text Link to Item
  - Kozubowski, L., K. Saito, J. M. Johnson, A. S. Howell, and D. J. Lew. “Response: GEF localization, not just activation, is needed for yeast polarity establishment.” Current Biology 19, no. 5 (March 10, 2009). https://doi.org/10.1016/j.cub.2009.01.022.
    Full Text
  - Lew, Daniel J., and Michael P. Rout. “Cell structure and dynamics..” Curr Opin Cell Biol 21, no. 1 (February 2009): 1–3. https://doi.org/10.1016/j.ceb.2009.01.010.
    Full Text Link to Item
  - Kozubowski, Lukasz, Koji Saito, Jayme M. Johnson, Audrey S. Howell, Trevin R. Zyla, and Daniel J. Lew. “Symmetry-breaking polarization driven by a Cdc42p GEF-PAK complex..” Curr Biol 18, no. 22 (November 25, 2008): 1719–26. https://doi.org/10.1016/j.cub.2008.09.060.
    Full Text Link to Item
  - Szkotnicki, Lee, John M. Crutchley, Trevin R. Zyla, Elaine S. G. Bardes, and Daniel J. Lew. “The checkpoint kinase Hsl1p is activated by Elm1p-dependent phosphorylation..” Mol Biol Cell 19, no. 11 (November 2008): 4675–86. https://doi.org/10.1091/mbc.e08-06-0663.
    Full Text Link to Item
  - Keaton, Mignon A., Lee Szkotnicki, Aron R. Marquitz, Jake Harrison, Trevin R. Zyla, and Daniel J. Lew. “Nucleocytoplasmic trafficking of G2/M regulators in yeast..” Mol Biol Cell 19, no. 9 (September 2008): 4006–18. https://doi.org/10.1091/mbc.e08-03-0286.
    Full Text Link to Item
  - Lew, Daniel J., Daniel J. Burke, and Anindya Dutta. “The immortal strand hypothesis: how could it work?.” Cell 133, no. 1 (April 4, 2008): 21–23. https://doi.org/10.1016/j.cell.2008.03.016.
    Full Text Link to Item
  - York, John D., and Daniel J. Lew. “IP7 guards the CDK gate..” Nat Chem Biol 4, no. 1 (January 2008): 16–17. https://doi.org/10.1038/nchembio0108-16.
    Full Text Link to Item
  - Tong, Zongtian, Xiang-Dong Gao, Audrey S. Howell, Indrani Bose, Daniel J. Lew, and Erfei Bi. “Adjacent positioning of cellular structures enabled by a Cdc42 GTPase-activating protein-mediated zone of inhibition..” J Cell Biol 179, no. 7 (December 31, 2007): 1375–84. https://doi.org/10.1083/jcb.200705160.
    Full Text Link to Item
  - Keaton, Mignon A., Elaine S. G. Bardes, Aron R. Marquitz, Christopher D. Freel, Trevin R. Zyla, Johannes Rudolph, and Daniel J. Lew. “Differential susceptibility of yeast S and M phase CDK complexes to inhibitory tyrosine phosphorylation..” Curr Biol 17, no. 14 (July 17, 2007): 1181–89. https://doi.org/10.1016/j.cub.2007.05.075.
    Full Text Link to Item
  - Haase, Steven B., and Daniel J. Lew. “Microtubule organization: cell shape is destiny..” Curr Biol 17, no. 7 (April 3, 2007): R249–51. https://doi.org/10.1016/j.cub.2007.02.003.
    Full Text Link to Item
  - Keaton, Mignon A., and Daniel J. Lew. “Eavesdropping on the cytoskeleton: progress and controversy in the yeast morphogenesis checkpoint..” Curr Opin Microbiol 9, no. 6 (December 2006): 540–46. https://doi.org/10.1016/j.mib.2006.10.004.
    Full Text Link to Item
  - Lew, Daniel J. “Yeast polarity: negative feedback shifts the focus..” Curr Biol 15, no. 24 (December 20, 2005): R994–96. https://doi.org/10.1016/j.cub.2005.11.048.
    Full Text Link to Item
  - McNulty, John J., and Daniel J. Lew. “Swe1p responds to cytoskeletal perturbation, not bud size, in S. cerevisiae..” Curr Biol 15, no. 24 (December 20, 2005): 2190–98. https://doi.org/10.1016/j.cub.2005.11.039.
    Full Text Link to Item
  - Gladfelter, Amy S., Lukasz Kozubowski, Trevin R. Zyla, and Daniel J. Lew. “Interplay between septin organization, cell cycle and cell shape in yeast..” J Cell Sci 118, no. Pt 8 (April 15, 2005): 1617–28. https://doi.org/10.1242/jcs.02286.
    Full Text Link to Item
  - Irazoqui, Javier E., Audrey S. Howell, Chandra L. Theesfeld, and Daniel J. Lew. “Opposing roles for actin in Cdc42p polarization..” Mol Biol Cell 16, no. 3 (March 2005): 1296–1304. https://doi.org/10.1091/mbc.e04-05-0430.
    Full Text Link to Item
  - Gladfelter, Amy S., Trevin R. Zyla, and Daniel J. Lew. “Genetic interactions among regulators of septin organization..” Eukaryot Cell 3, no. 4 (August 2004): 847–54. https://doi.org/10.1128/EC.3.4.847-854.2004.
    Full Text Link to Item
  - Irazoqui, Javier E., Amy S. Gladfelter, and Daniel J. Lew. “Cdc42p, GTP hydrolysis, and the cell's sense of direction..” Cell Cycle 3, no. 7 (July 2004): 861–64.
    Link to Item
  - Irazoqui, Javier E., and Daniel J. Lew. “Polarity establishment in yeast..” J Cell Sci 117, no. Pt 11 (May 1, 2004): 2169–71. https://doi.org/10.1242/jcs.00953.
    Full Text Link to Item
  - Harrison, Jacob C., Trevin R. Zyla, Elaine S. G. Bardes, and Daniel J. Lew. “Stress-specific activation mechanisms for the "cell integrity" MAPK pathway..” J Biol Chem 279, no. 4 (January 23, 2004): 2616–22. https://doi.org/10.1074/jbc.M306110200.
    Full Text Link to Item
  - Irazoqui, Javier E., Amy S. Gladfelter, and Daniel J. Lew. “Scaffold-mediated symmetry breaking by Cdc42p..” Nat Cell Biol 5, no. 12 (December 2003): 1062–70. https://doi.org/10.1038/ncb1068.
    Full Text Link to Item
  - Lew, Daniel J. “The morphogenesis checkpoint: how yeast cells watch their figures..” Curr Opin Cell Biol 15, no. 6 (December 2003): 648–53. https://doi.org/10.1016/j.ceb.2003.09.001.
    Full Text Link to Item
  - Theesfeld, Chandra L., Trevin R. Zyla, Elaine G. S. Bardes, and Daniel J. Lew. “A monitor for bud emergence in the yeast morphogenesis checkpoint..” Mol Biol Cell 14, no. 8 (August 2003): 3280–91. https://doi.org/10.1091/mbc.e03-03-0154.
    Full Text Link to Item
  - Lew, Daniel J., and Daniel J. Burke. “The spindle assembly and spindle position checkpoints..” Annu Rev Genet 37 (2003): 251–82. https://doi.org/10.1146/annurev.genet.37.042203.120656.
    Full Text Link to Item
  - McMillan, John N., Chandra L. Theesfeld, Jacob C. Harrison, Elaine S. G. Bardes, and Daniel J. Lew. “Determinants of Swe1p degradation in Saccharomyces cerevisiae..” Mol Biol Cell 13, no. 10 (October 2002): 3560–75. https://doi.org/10.1091/mbc.e02-05-0283.
    Full Text Link to Item
  - Marquitz, Aron R., Jacob C. Harrison, Indrani Bose, Trevin R. Zyla, John N. McMillan, and Daniel J. Lew. “The Rho-GAP Bem2p plays a GAP-independent role in the morphogenesis checkpoint..” Embo J 21, no. 15 (August 1, 2002): 4012–25. https://doi.org/10.1093/emboj/cdf416.
    Full Text Link to Item
  - Lew, Daniel J. “Formin' actin filament bundles..” Nat Cell Biol 4, no. 2 (February 2002): E29–30. https://doi.org/10.1038/ncb0202-e29.
    Full Text Link to Item
  - Gladfelter, Amy S., Indrani Bose, Trevin R. Zyla, Elaine S. G. Bardes, and Daniel J. Lew. “Septin ring assembly involves cycles of GTP loading and hydrolysis by Cdc42p..” J Cell Biol 156, no. 2 (January 21, 2002): 315–26. https://doi.org/10.1083/jcb.200109062.
    Full Text Link to Item
  - Gladfelter, A. S., J. R. Pringle, and D. J. Lew. “The septin cortex at the yeast mother-bud neck..” Curr Opin Microbiol 4, no. 6 (December 2001): 681–89. https://doi.org/10.1016/s1369-5274(01)00269-7.
    Full Text Link to Item
  - Adamo, J. E., J. J. Moskow, A. S. Gladfelter, D. Viterbo, D. J. Lew, and P. J. Brennwald. “Yeast Cdc42 functions at a late step in exocytosis, specifically during polarized growth of the emerging bud..” J Cell Biol 155, no. 4 (November 12, 2001): 581–92. https://doi.org/10.1083/jcb.200106065.
    Full Text Link to Item
  - Marquitz, A. R., J. C. Harrison, C. L. Theesfeld, E. S. G. Bardes, and D. J. Lew. “The Rho GAP Bem2p plays a role in the morphogenesis checkpoint of Saccharomyces cerevisiae.” Molecular Biology of the Cell 12 (November 1, 2001): 408A-408A.
    Link to Item
  - Theesfeld, C. L., and D. J. Lew. “Septin-dependent monitoring of local cell shape in the yeast morphogenesis checkpoint.” Molecular Biology of the Cell 12 (November 1, 2001): 142A-143A.
    Link to Item
  - Adamo, J. E., J. J. Moskow, A. S. Gladfelter, D. Viterbo, D. J. Lew, and P. J. Brennwald. “Yeast Cdc42 functions at a late step in exocytosis, specifically during polarized growth of the emerging bud.” Journal of Cell Biology 155, no. 3 (October 29, 2001): 581–92.
  - Theesfeld, C. L., E. Bardes, and D. J. Lew. “Hsl1 as a sensor of cell shape..” Yeast 18 (August 1, 2001): S142–S142.
    Link to Item
  - Gladfelter, A. S., J. J. Moskow, T. R. Zyla, and D. J. Lew. “Isolation and characterization of effector-loop mutants of CDC42 in yeast..” Mol Biol Cell 12, no. 5 (May 2001): 1239–55. https://doi.org/10.1091/mbc.12.5.1239.
    Full Text Link to Item
  - Harrison, J. C., E. S. Bardes, Y. Ohya, and D. J. Lew. “A role for the Pkc1p/Mpk1p kinase cascade in the morphogenesis checkpoint..” Nat Cell Biol 3, no. 4 (April 2001): 417–20. https://doi.org/10.1038/35070104.
    Full Text Link to Item
  - Bose, I., J. E. Irazoqui, J. J. Moskow, E. S. Bardes, T. R. Zyla, and D. J. Lew. “Assembly of scaffold-mediated complexes containing Cdc42p, the exchange factor Cdc24p, and the effector Cla4p required for cell cycle-regulated phosphorylation of Cdc24p..” J Biol Chem 276, no. 10 (March 9, 2001): 7176–86. https://doi.org/10.1074/jbc.M010546200.
    Full Text Link to Item
  - Yeh, E., C. Yang, E. Chin, P. Maddox, E. D. Salmon, D. J. Lew, and K. Bloom. “Dynamic positioning of mitotic spindles in yeast: role of microtubule motors and cortical determinants..” Mol Biol Cell 11, no. 11 (November 2000): 3949–61. https://doi.org/10.1091/mbc.11.11.3949.
    Full Text Link to Item
  - Moskow, J. J., A. S. Gladfelter, R. E. Lamson, P. M. Pryciak, and D. J. Lew. “Role of Cdc42p in pheromone-stimulated signal transduction in Saccharomyces cerevisiae..” Mol Cell Biol 20, no. 20 (October 2000): 7559–71. https://doi.org/10.1128/mcb.20.20.7559-7571.2000.
    Full Text Link to Item
  - Longtine, M. S., C. L. Theesfeld, J. N. McMillan, E. Weaver, J. R. Pringle, and D. J. Lew. “Septin-dependent assembly of a cell cycle-regulatory module in Saccharomyces cerevisiae..” Mol Cell Biol 20, no. 11 (June 2000): 4049–61. https://doi.org/10.1128/mcb.20.11.4049-4061.2000.
    Full Text Link to Item
  - Lew, D. J. “Cell-cycle checkpoints that ensure coordination between nuclear and cytoplasmic events in Saccharomyces cerevisiae..” Curr Opin Genet Dev 10, no. 1 (February 2000): 47–53. https://doi.org/10.1016/s0959-437x(99)00051-9.
    Full Text Link to Item
  - Kim, J. M., K. H. Chang, Y. H. Choi, Y. G. Song, S. M. Kang, T. Y. Yoon, J. M. Choi, S. Y. Park, and D. J. Lew. “Seroprevalence of HTLV-I in Cheju Island, a Korean island adjacent to the endemic area of Japan..” J Acquir Immune Defic Syndr 22, no. 4 (December 1, 1999): 409–12. https://doi.org/10.1097/00126334-199912010-00016.
    Full Text Link to Item
  - Gladfelter, A. S., J. J. Moskow, and D. J. Lew. “Analysis of cdc42effector loop mutants in budding yeast.” Molecular Biology of the Cell 10 (November 1, 1999): 54A-54A.
    Link to Item
  - Lew, D. J. “Cell cycle and checkpoint control of Swe1p localization and degradation..” Molecular Biology of the Cell 10 (November 1, 1999): 3A-3A.
    Link to Item
  - McMillan, J. N., M. S. Longtine, R. A. Sia, C. L. Theesfeld, E. S. Bardes, J. R. Pringle, and D. J. Lew. “The morphogenesis checkpoint in Saccharomyces cerevisiae: cell cycle control of Swe1p degradation by Hsl1p and Hsl7p..” Mol Cell Biol 19, no. 10 (October 1999): 6929–39. https://doi.org/10.1128/mcb.19.10.6929.
    Full Text Link to Item
  - Theesfeld, C. L., J. E. Irazoqui, K. Bloom, and D. J. Lew. “The role of actin in spindle orientation changes during the Saccharomyces cerevisiae cell cycle..” J Cell Biol 146, no. 5 (September 6, 1999): 1019–32. https://doi.org/10.1083/jcb.146.5.1019.
    Full Text Link to Item
  - McMillan, J. N., R. A. Sia, E. S. Bardes, and D. J. Lew. “Phosphorylation-independent inhibition of Cdc28p by the tyrosine kinase Swe1p in the morphogenesis checkpoint..” Mol Cell Biol 19, no. 9 (September 1999): 5981–90. https://doi.org/10.1128/mcb.19.9.5981.
    Full Text Link to Item
  - Sia, R. A., E. S. Bardes, and D. J. Lew. “Control of Swe1p degradation by the morphogenesis checkpoint..” Embo J 17, no. 22 (November 16, 1998): 6678–88. https://doi.org/10.1093/emboj/17.22.6678.
    Full Text Link to Item
  - McMillan, J. N., R. A. Sia, and D. J. Lew. “A morphogenesis checkpoint monitors the actin cytoskeleton in yeast..” J Cell Biol 142, no. 6 (September 21, 1998): 1487–99. https://doi.org/10.1083/jcb.142.6.1487.
    Full Text Link to Item
  - Bi, E., P. Maddox, D. J. Lew, E. D. Salmon, J. N. McMillan, E. Yeh, and J. R. Pringle. “Involvement of an actomyosin contractile ring in Saccharomyces cerevisiae cytokinesis..” J Cell Biol 142, no. 5 (September 7, 1998): 1301–12. https://doi.org/10.1083/jcb.142.5.1301.
    Full Text Link to Item
  - Kaiser, P., R. A. Sia, E. G. Bardes, D. J. Lew, and S. I. Reed. “Cdc34 and the F-box protein Met30 are required for degradation of the Cdk-inhibitory kinase Swe1..” Genes Dev 12, no. 16 (August 15, 1998): 2587–97. https://doi.org/10.1101/gad.12.16.2587.
    Full Text Link to Item
  - Haase, S. B., and D. J. Lew. “Flow cytometric analysis of DNA content in budding yeast..” Methods Enzymol 283 (1997): 322–32. https://doi.org/10.1016/s0076-6879(97)83026-1.
    Full Text Link to Item
  - Lew, D. J., and S. Kornbluth. “Regulatory roles of cyclin dependent kinase phosphorylation in cell cycle control..” Curr Opin Cell Biol 8, no. 6 (December 1996): 795–804. https://doi.org/10.1016/s0955-0674(96)80080-9.
    Full Text Link to Item
  - McMillan, J. N., and D. J. Lew. “Genetic screen to uncover proteins involved in the morphogenesis cell cycle checkpoint in budding yeast..” Molecular Biology of the Cell 7 (December 1, 1996): 2077–2077.
    Link to Item
  - Sia, R. A., H. A. Herald, and D. J. Lew. “Cdc28 tyrosine phosphorylation and the morphogenesis checkpoint in budding yeast..” Mol Biol Cell 7, no. 11 (November 1996): 1657–66. https://doi.org/10.1091/mbc.7.11.1657.
    Full Text Link to Item
  - Lew, D. J., and S. I. Reed. “A cell cycle checkpoint monitors cell morphogenesis in budding yeast..” J Cell Biol 129, no. 3 (May 1995): 739–49. https://doi.org/10.1083/jcb.129.3.739.
    Full Text Link to Item
  - Parks, B., S. Spielman, J. Orenstein, D. T. Nemeth, F. Ludwig, J. Clarke, P. Merchant, and D. J. Lew. “Phase-sensitive measurements of vortex dynamics in the terahertz domain..” Phys Rev Lett 74, no. 16 (April 17, 1995): 3265–68. https://doi.org/10.1103/PhysRevLett.74.3265.
    Full Text Link to Item
  - Lew, D. J., and S. I. Reed. “Cell cycle control of morphogenesis in budding yeast..” Curr Opin Genet Dev 5, no. 1 (February 1995): 17–23. https://doi.org/10.1016/s0959-437x(95)90048-9.
    Full Text Link to Item
  - Spielman, S., B. Parks, J. Orenstein, D. T. Nemeth, F. Ludwig, J. Clarke, P. Merchant, and D. J. Lew. “Observation of the quasiparticle Hall effect in superconducting YBa2Cu3O7- delta..” Phys Rev Lett 73, no. 11 (September 12, 1994): 1537–40. https://doi.org/10.1103/PhysRevLett.73.1537.
    Full Text Link to Item
  - Stueland, C. S., D. J. Lew, M. J. Cismowski, and S. I. Reed. “Full activation of p34CDC28 histone H1 kinase activity is unable to promote entry into mitosis in checkpoint-arrested cells of the yeast Saccharomyces cerevisiae..” Mol Cell Biol 13, no. 6 (June 1993): 3744–55. https://doi.org/10.1128/mcb.13.6.3744.
    Full Text Link to Item
  - Lew, D. J., and S. I. Reed. “Morphogenesis in the yeast cell cycle: regulation by Cdc28 and cyclins..” J Cell Biol 120, no. 6 (March 1993): 1305–20. https://doi.org/10.1083/jcb.120.6.1305.
    Full Text Link to Item
  - Lew, D. J., N. J. Marini, and S. I. Reed. “A suppressor of cln3 for size control..” Cell 72, no. 4 (February 26, 1993): 488–89. https://doi.org/10.1016/0092-8674(93)90068-2.
    Full Text Link to Item
  - Richardson, H., D. J. Lew, M. Henze, K. Sugimoto, and S. I. Reed. “Cyclin-B homologs in Saccharomyces cerevisiae function in S phase and in G2..” Genes Dev 6, no. 11 (November 1992): 2021–34. https://doi.org/10.1101/gad.6.11.2021.
    Full Text Link to Item
  - Lew, D. J., N. J. Marini, and S. I. Reed. “Different G1 cyclins control the timing of cell cycle commitment in mother and daughter cells of the budding yeast S. cerevisiae..” Cell 69, no. 2 (April 17, 1992): 317–27. https://doi.org/10.1016/0092-8674(92)90412-6.
    Full Text Link to Item
  - Lew, D. J., and S. I Reed. “A proliferation of cyclins..” Trends Cell Biol 2, no. 3 (March 1992): 77–81. https://doi.org/10.1016/0962-8924(92)90076-y.
    Full Text Link to Item
  - Lu, X. P., K. S. Koch, D. J. Lew, V. Dulic, J. Pines, S. I. Reed, T. Hunter, and H. L. Leffert. “Induction of cyclin mRNA and cyclin-associated histone H1 kinase during liver regeneration..” J Biol Chem 267, no. 5 (February 15, 1992): 2841–44.
    Link to Item
  - Reed, S. I., V. Dulic, D. J. Lew, H. E. Richardson, and C. Wittenberg. “G1 control in yeast and animal cells..” Ciba Found Symp 170 (1992): 7–15. https://doi.org/10.1002/9780470514320.ch2.
    Full Text Link to Item
  - Decker, T., D. J. Lew, and J. E. Darnell. “Two distinct alpha-interferon-dependent signal transduction pathways may contribute to activation of transcription of the guanylate-binding protein gene..” Mol Cell Biol 11, no. 10 (October 1991): 5147–53. https://doi.org/10.1128/mcb.11.10.5147.
    Full Text Link to Item
  - Lew, D. J., V. Dulić, and S. I. Reed. “Isolation of three novel human cyclins by rescue of G1 cyclin (Cln) function in yeast..” Cell 66, no. 6 (September 20, 1991): 1197–1206. https://doi.org/10.1016/0092-8674(91)90042-w.
    Full Text Link to Item
  - Lew, D. J., and S. M. Simon. “Characterization of constitutive exocytosis in the yeast Saccharomyces cerevisiae..” J Membr Biol 123, no. 3 (September 1991): 261–68. https://doi.org/10.1007/bf01870409.
    Full Text Link to Item
  - Ghiara, J. B., H. E. Richardson, K. Sugimoto, M. Henze, D. J. Lew, C. Wittenberg, and S. I. Reed. “A cyclin B homolog in S. cerevisiae: chronic activation of the Cdc28 protein kinase by cyclin prevents exit from mitosis..” Cell 65, no. 1 (April 5, 1991): 163–74. https://doi.org/10.1016/0092-8674(91)90417-w.
    Full Text Link to Item
  - Decker, T., D. J. Lew, J. Mirkovitch, and J. E. Darnell. “Cytoplasmic activation of GAF, an IFN-gamma-regulated DNA-binding factor..” Embo J 10, no. 4 (April 1991): 927–32.
    Link to Item
  - Lew, D. J., T. Decker, I. Strehlow, and J. E. Darnell. “Overlapping elements in the guanylate-binding protein gene promoter mediate transcriptional induction by alpha and gamma interferons..” Mol Cell Biol 11, no. 1 (January 1991): 182–91. https://doi.org/10.1128/mcb.11.1.182.
    Full Text Link to Item
  - Reed, S. I., C. Wittenberg, D. J. Lew, V. Dulic, and M. Henze. “G1 control in yeast and animal cells..” Cold Spring Harb Symp Quant Biol 56 (1991): 61–67. https://doi.org/10.1101/sqb.1991.056.01.009.
    Full Text Link to Item
  - Reed, S. I., C. Wittenberg, D. J. Lew, V. Dulic, and M. Henze. “G₁ control in yeast and animal cells.” Cold Spring Harbor Symposia on Quantitative Biology 56 (1991): 61–68.
  - Levy, D. E., D. J. Lew, T. Decker, D. S. Kessler, and J. E. Darnell. “Synergistic interaction between interferon-alpha and interferon-gamma through induced synthesis of one subunit of the transcription factor ISGF3..” Embo J 9, no. 4 (April 1990): 1105–11.
    Link to Item
  - Lew, D. J., T. Decker, and J. E. Darnell. “Alpha interferon and gamma interferon stimulate transcription of a single gene through different signal transduction pathways..” Mol Cell Biol 9, no. 12 (December 1989): 5404–11. https://doi.org/10.1128/mcb.9.12.5404.
    Full Text Link to Item
  - Decker, T., D. J. Lew, Y. S. Cheng, D. E. Levy, and J. E. Darnell. “Interactions of alpha- and gamma-interferon in the transcriptional regulation of the gene encoding a guanylate-binding protein..” Embo J 8, no. 7 (July 1989): 2009–14.
    Link to Item
  - Bookchin, R. M., D. J. Lew, T. Balazs, Y. Ueda, and V. L. Lew. “Dehydration and delayed proton equilibria of red blood cells suspended in isosmotic phosphate buffers. Implications for studies of sickled cells..” J Lab Clin Med 104, no. 6 (December 1984): 855–66.
    Link to Item
- Book Sections
  - Lew, D. J. “Cell Cycle.” In Brenner’s Encyclopedia of Genetics: Second Edition, 456–64, 2013. https://doi.org/10.1016/B978-0-12-374984-0.00206-0.
    Full Text
- Conference Papers
  - Ghose, D., D. J. Lew, and T. C. Elston. “Chemotropism in yeast..” In Molecular Biology of the Cell, Vol. 28. AMER SOC CELL BIOLOGY, 2017.
    Link to Item
  - Daniels, C. N., and D. J. Lew. “A novel role for Cdc42 effectors Gic1/2 in cell polarity..” In Molecular Biology of the Cell, Vol. 27. AMER SOC CELL BIOLOGY, 2016.
    Link to Item
  - Kang, H., and D. J. Lew. “Elm1 regulates Hsl1/Hsl7 interaction in the morphogenesis checkpoint.” In Molecular Biology of the Cell, Vol. 26. AMER SOC CELL BIOLOGY, 2015.
    Link to Item
  - Woods, B., H. Lai, C. Wu, C. Kuo, T. Zyla, and D. J. Lew. “Polarity establishment requires localized activation of Cdc42 by its GEF.” In Molecular Biology of the Cell, Vol. 26. AMER SOC CELL BIOLOGY, 2015.
    Link to Item
  - Lai, H., T. Zyla, and D. J. Lew. “Regulation of pheromone-induced cell polarization by the polarisome proteins in yeast..” In Molecular Biology of the Cell, Vol. 25. AMER SOC CELL BIOLOGY, 2014.
    Link to Item
  - Woods, B., C. Wu, T. Zyla, N. Savage, and D. J. Lew. “A GDI-independent mechanism to concentrate the Rho-GTPase Cdc42 during polarity establishment..” In Molecular Biology of the Cell, Vol. 25. AMER SOC CELL BIOLOGY, 2014.
    Link to Item
  - Chen, H., A. S. Howell, H. Kang, and D. J. Lew. “How does Cdc42p polarize actin cables?.” In Molecular Biology of the Cell, Vol. 22. AMER SOC CELL BIOLOGY, 2011.
    Link to Item
  - Howell, A. S., J. E. Irazoqui, C. L. Theesfeld, and D. J. Lew. “Opposing roles for actin in Cdc42p polarization.” In Molecular Biology of the Cell, 15:335A-335A. AMER SOC CELL BIOLOGY, 2004.
    Link to Item
  - Kozubowski, L., J. R. Larson, D. J. Lew, and K. Tatchell. “Exploring the basis for the asymmetry of septin-dependent proteins at the mother-bud neck in Saccharomyces cerevisiae.” In Molecular Biology of the Cell, 15:46A-46A. AMER SOC CELL BIOLOGY, 2004.
    Link to Item
  - McNulty, J. J., and D. J. Lew. “Bud size doesnt matter.” In Molecular Biology of the Cell, 15:20A-20A. AMER SOC CELL BIOLOGY, 2004.
    Link to Item
  - Irazoqui, J. E., and D. J. Lew. “The role of F-actin in Cdc42p polarization..” In Yeast, 20:S67–S67. JOHN WILEY & SONS LTD, 2003.
    Link to Item
  - Harrison, J. C., E. G. Bardes, T. R. Zyla, and D. J. Lew. “Stress-specific activation mechanisms for the "cell integrity" MAPK pathway in Saccharomyces cerevisiae.” In Molecular Biology of the Cell, 13:294A-294A. AMER SOC CELL BIOLOGY, 2002.
    Link to Item
  - Irazoqui, J. E., and D. J. Lew. “Bem1p is required for Cdc42p polarization in the absence of bud site selection.” In Molecular Biology of the Cell, 13:200A-200A. AMER SOC CELL BIOLOGY, 2002.
    Link to Item
Teaching & Mentoring
Recent Courses

Some information on this profile has been compiled automatically from Duke databases and external sources. (Our About page explains how this works.) If you see a problem with the information, please write to Scholars@Duke and let us know. We will reply promptly.

Education, Training, & Certifications

Duke Appointment History

Awards & Honors

Subject Headings

Selected Grants

Selected Publications

Academic Articles

Book Sections

Conference Papers

Recent Courses