Daniel Raphael Saban

Journal article Bioinformatics · February 15, 2020 Featured Publication MOTIVATION: Alternative polyadenylation (APA) plays a key post-transcriptional regulatory role in mRNA stability and functions in eukaryotes. Single cell RNA-seq (scRNA-seq) is a powerful tool to discover cellular heterogeneity at gene expression level. Gi ... Full text Link to item Cite

Large-scale death of retinal astrocytes during normal development is non-apoptotic and implemented by microglia.

Journal article PLoS Biol · October 2019 Featured Publication Naturally occurring cell death is a fundamental developmental mechanism for regulating cell numbers and sculpting developing organs. This is particularly true in the nervous system, where large numbers of neurons and oligodendrocytes are eliminated via apo ... Full text Link to item Cite

Complement and CD4+ T cells drive context-specific corneal sensory neuropathy.

Journal article Elife · August 15, 2019 Featured Publication Whether complement dysregulation directly contributes to the pathogenesis of peripheral nervous system diseases, including sensory neuropathies, is unclear. We addressed this important question in a mouse model of ocular HSV-1 infection, where sensory nerv ... Full text Link to item Cite

PRCD is essential for high-fidelity photoreceptor disc formation.

Journal article Proc Natl Acad Sci U S A · June 25, 2019 Featured Publication Progressive rod-cone degeneration (PRCD) is a small protein residing in the light-sensitive disc membranes of the photoreceptor outer segment. Until now, the function of PRCD has remained enigmatic despite multiple demonstrations that its mutations cause b ... Full text Link to item Cite

Immune cells in the retina and choroid: Two different tissue environments that require different defenses and surveillance.

Journal article Prog Retin Eye Res · May 2019 Featured Publication In the eye immune defenses must take place in a plethora of differing microenvironments ranging from the corneal and conjunctival epithelia facing the external environment to the pigmented connective tissue of the uveal tract containing smooth muscle, bloo ... Full text Link to item Cite

Microglial Function Is Distinct in Different Anatomical Locations during Retinal Homeostasis and Degeneration.

Journal article Immunity · March 19, 2019 Featured Publication Microglia from different nervous system regions are molecularly and anatomically distinct, but whether they also have different functions is unknown. We combined lineage tracing, single-cell transcriptomics, and electrophysiology of the mouse retina and sh ... Full text Link to item Cite

Resolvin D1 treatment on goblet cell mucin and immune responses in the chronic allergic eye disease (AED) model.

Journal article Mucosal Immunol · January 2019 Featured Publication Severe, chronic eye allergy is an understudied, vision-threatening condition. Treatments remain limited. We used a mouse model of severe allergic eye disease (AED) to determine whether topical application of the pro-resolution mediator Resolvin D1 (RvD1) t ... Full text Link to item Cite

Fate Mapping In Vivo to Distinguish Bona Fide Microglia Versus Recruited Monocyte-Derived Macrophages in Retinal Disease.

Journal article Methods Mol Biol · 2019 Featured Publication With the new understanding that adult microglia in mice have embryonic origins and are maintained in situ throughout life, it has become pertinent to now understand how these unique cells differ from monocyte-derived macrophages. The latter are recruited i ... Full text Link to item Cite

SYK inhibitor entospletinib prevents ocular and skin GVHD in mice.

Journal article JCI Insight · October 4, 2018 Featured Publication Graft-versus-host disease (GVHD) is a major complication of hematopoietic stem cell transplantation (HCT). The tyrosine kinase SYK contributes to both acute and chronic GVHD development, making it an attractive target for GVHD prevention. Entospletinib (EN ... Full text Link to item Cite

New concepts in macrophage ontogeny in the adult neural retina.

Journal article Cell Immunol · August 2018 Featured Publication The number of neurons dedicated to vision itself is thought to be greater than the sum of the four other senses combined. Yet, little attention has been payed to the retina as compared to elsewhere in the central nervous system with respect to microglia, t ... Full text Link to item Cite

Neutrophils cause obstruction of eyelid sebaceous glands in inflammatory eye disease in mice.

Journal article Sci Transl Med · July 25, 2018 Featured Publication Meibomian glands (MGs) are sebaceous glands of the eyelid margin that secrete lipids needed to avert tear evaporation and to help maintain ocular surface homeostasis. Obstruction of MGs or other forms of MG dysfunction can promote chronic diseases of the o ... Full text Link to item Cite

A Commencement for Eye Commensals.

Journal article Immunity · July 18, 2017 Featured Publication "Paucibacterial" levels of the normal eye surface have left immunologists wondering whether a true microbiome exists there. In this issue of Immunity, St. Leger et al. (2017) address this head-on, discovering a naturally existing commensal in mice that ind ... Full text Link to item Cite

New insights into mononuclear phagocyte biology from the visual system.

Journal article Nat Rev Immunol · May 2017 Featured Publication Major advances in mononuclear phagocyte biology have been made but key questions pertinent to their roles in health and disease remain, including in the visual system. One problem concerns how dendritic cells can trigger immune responses from certain tight ... Full text Link to item Cite

Recipient-Derived BAFF and Alloantigen Synergistically Activate B Cells in Murine Chronic Gvhd

Conference Blood · December 2, 2016 Featured Publication AbstractIncreased B cell-activating factor (BAFF) and aberrant B cell survival and activation are associated with chronic graft versus host disease (cGVHD) in patients. Whether excessive BAFF production has ... Full text Cite

Classical dendritic cells mediate fibrosis directly via the retinoic acid pathway in severe eye allergy.

Journal article JCI Insight · August 4, 2016 Featured Publication Fibrosis is a shared end-stage pathway to lung, liver, and heart failure. In the ocular mucosa (conjunctiva), fibrosis leads to blindness in trachoma, pemphigoid, and allergy. The indirect fibrogenic role of DCs via T cell activation and inflammatory cell ... Full text Link to item Cite

Aldehyde dehydrogenase inhibition blocks mucosal fibrosis in human and mouse ocular scarring.

Journal article JCI Insight · August 4, 2016 Featured Publication Mucous membrane pemphigoid (MMP) is a systemic mucosal scarring disease, commonly causing blindness, for which there is no antifibrotic therapy. Aldehyde dehydrogenase family 1 (ALDH1) is upregulated in both ocular MMP (OMMP) conjunctiva and cultured fibro ... Full text Link to item Cite

Secondary allergic T cell responses are regulated by dendritic cell-derived thrombospondin-1 in the setting of allergic eye disease.

Journal article J Leukoc Biol · August 2016 Featured Publication Allergic eye disease, as in most forms of atopy, ranges in severity among individuals from immediate hypersensitivity to a severe and debilitating chronic disease. Dendritic cells play a key role in stimulating pathogenic T cells in allergen re-exposure, o ... Full text Link to item Cite

Fate mapping reveals that microglia and recruited monocyte-derived macrophages are definitively distinguishable by phenotype in the retina.

Journal article Sci Rep · February 9, 2016 Featured Publication The recent paradigm shift that microglia are yolk sac-derived, not hematopoietic-derived, is reshaping our knowledge about the isolated role of microglia in CNS diseases, including degenerative conditions of the retina. However, unraveling microglial-speci ... Full text Link to item Cite

Regulation of age-related macular degeneration-like pathology by complement factor H.

Journal article Proc Natl Acad Sci U S A · June 9, 2015 Featured Publication Complement factor H (CFH) is a major susceptibility gene for age-related macular degeneration (AMD); however, its impact on AMD pathobiology is unresolved. Here, the role of CFH in the development of AMD pathology in vivo was interrogated by analyzing aged ... Full text Link to item Cite

Involvement of corneal lymphangiogenesis in a mouse model of allergic eye disease.

Journal article Invest Ophthalmol Vis Sci · May 2015 Featured Publication PURPOSE: The contribution of lymphangiogenesis (LA) to allergy has received considerable attention and therapeutic inhibition of this process via targeting VEGF has been considered. Likewise, certain inflammatory settings affecting the ocular mucosa can tr ... Full text Link to item Cite

CCR7 is critical for the induction and maintenance of Th17 immunity in dry eye disease.

Journal article Invest Ophthalmol Vis Sci · August 19, 2014 Featured Publication PURPOSE: We characterized antigen-presenting cell (APC)-relevant chemokine receptor expression in dry eye disease (DED), and investigated the effect of topical CC chemokine receptor (CCR)-7 blockade specifically on Th17 cell immunity and dry eye disease se ... Full text Link to item Cite