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David M. Tobin

Professor of Molecular Genetics and Microbiology
Molecular Genetics and Microbiology
Duke Box 3020, Durham, NC 27710
207 Research Drive, 207A Jones Building, Box 3020, Durham, NC 27710

Overview


Tuberculosis: Mycobacterial Pathogenesis and Host Susceptibility

Tuberculosis kills 1.5 million people annually. Our laboratory aims to understand the intricate interplay between mycobacteria and their hosts using a combination of model organism genetics, human genetics, pharmacology and high-resolution microscopy. By identifying key pathways utilized by the infecting bacteria and the host innate immune system, we hope to discover new therapeutic targets and interventions to combat this enduringly destructive disease.

Using a Mycobacterium/zebrafish model, we have identified new host susceptibility loci for tuberculosis. Zebrafish are natural hosts to Mycobacterium marinum, the closest relative of the Mycobacterium tuberculosis complex. Because zebrafish embryos and larvae are optically transparent, we are able to visualize the complex details of mycobacterial pathogenesis in whole, live animals. The facile genetics of the zebrafish allow us to map and positionally clone affected host susceptibility genes. In addition, zebrafish larvae are remarkably permeable to small molecules, providing a platform for whole-animal pharmacological manipulation of specific host immune responses.

We have identified novel pathways that modulate susceptibility to tuberculosis. We have shown that genes identified in the zebrafish model are also important in human tuberculosis. We find robust associations of human variants in a specific eicosanoid pathway with susceptibility to both tuberculosis and leprosy.

We have active collaborations in both Vietnam and Guatemala. In Guatemala, we are working with the Clínica Familiar Luis Angel García and the Asociación de Salud Integral to support projects involving HIV-infected patients and to understand the dynamics of TB transmission in Central America.

Current Appointments & Affiliations


Professor of Molecular Genetics and Microbiology · 2023 - Present Molecular Genetics and Microbiology, Basic Science Departments
Professor of Cell Biology · 2023 - Present Cell Biology, Basic Science Departments
Professor in Integrative Immunobiology · 2023 - Present Integrative Immunobiology, Basic Science Departments

Education, Training & Certifications


University of California San Francisco, School of Medicine · 2002 Ph.D.

External Links


Tobin lab website