David M. Tobin | Scholars@Duke

David M. Tobin
Associate Professor of Molecular Genetics and Microbiology

Tuberculosis: Mycobacterial Pathogenesis and Host Susceptibility

Tuberculosis kills 1.5 million people annually. Our laboratory aims to understand the intricate interplay between mycobacteria and their hosts using a combination of model organism genetics, human genetics, pharmacology and high-resolution microscopy. By identifying key pathways utilized by the infecting bacteria and the host innate immune system, we hope to discover new therapeutic targets and interventions to combat this enduringly destructive disease.

Using a Mycobacterium/zebrafish model, we have identified new host susceptibility loci for tuberculosis. Zebrafish are natural hosts to Mycobacterium marinum, the closest relative of the Mycobacterium tuberculosis complex. Because zebrafish embryos and larvae are optically transparent, we are able to visualize the complex details of mycobacterial pathogenesis in whole, live animals. The facile genetics of the zebrafish allow us to map and positionally clone affected host susceptibility genes. In addition, zebrafish larvae are remarkably permeable to small molecules, providing a platform for whole-animal pharmacological manipulation of specific host immune responses.

We have identified novel pathways that modulate susceptibility to tuberculosis. We have shown that genes identified in the zebrafish model are also important in human tuberculosis. We find robust associations of human variants in a specific eicosanoid pathway with susceptibility to both tuberculosis and leprosy.

We have active collaborations in both Vietnam and Guatemala. In Guatemala, we are working with the Clínica Familiar Luis Angel García and the Asociación de Salud Integral to support projects involving HIV-infected patients and to understand the dynamics of TB transmission in Central America.

Current Appointments & Affiliations

Associate Professor of Molecular Genetics and Microbiology, Molecular Genetics and Microbiology, Basic Science Departments 2017
Associate Professor in Integrative Immunobiology, Integrative Immunobiology, Basic Science Departments 2017
Associate Professor of Cell Biology, Cell Biology, Basic Science Departments 2022

Contact Information

207 Research Drive, 207A Jones Building, Box 3020, Durham, NC 27710
Duke Box 3020, Durham, NC 27710
david.tobin@duke.edu (919) 684-9152
Tobin lab website

Background
Education, Training, & Certifications
- Ph.D., University of California San Francisco, School of Medicine 2002
Previous Appointments & Affiliations
- Assistant Professor of Molecular Genetics and Microbiology, Molecular Genetics and Microbiology, Basic Science Departments 2011 - 2017
- Assistant Professor in Immunology, Integrative Immunobiology, Basic Science Departments 2015 - 2017
Leadership & Clinical Positions at Duke
- Director of Graduate Studies, Department of Molecular Genetics and Microbiology, 2018-present
Recognition
In the News
- NOV 9, 2022
  
  Mysterious Outbreak of Bone-Eating TB Resembled an Ancestral Form
- NOV 26, 2019
  
  Seven From Duke Named Fellows of American Association for the Advancement of Science
- OCT 1, 2015
  
  Zebrafish Reveals Infection’s Sci-Fi-Worthy Creep Into the Brain
- FEB 16, 2015
  
  Researchers Discover Molecular Trigger of Inflammatory Bowel Disease
- NOV 24, 2014
  
  Drugs to Block Angiogenesis Could Provide New Treatment for TB
Awards & Honors
Expertise
Subject Headings
Global Scholarship
- Research
Research
External Relationships
- Company of Biologists
- Vlaams Instituut voor Biotechnologie (Belgium)
Publications & Artistic Works
Selected Publications
- Academic Articles
  - Brewer, W Jared, Ana María Xet-Mull, Anne Yu, Mollie I. Sweeney, Eric M. Walton, and David M. Tobin. “Macrophage NFATC2 mediates angiogenic signaling during mycobacterial infection.” Cell Rep 41, no. 11 (December 13, 2022): 111817. https://doi.org/10.1016/j.celrep.2022.111817.
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  - Saelens, Joseph W., Mollie I. Sweeney, Gopinath Viswanathan, Ana María Xet-Mull, Kristen L. Jurcic Smith, Dana M. Sisk, Daniel D. Hu, et al. “An ancestral mycobacterial effector promotes dissemination of infection.” Cell 185, no. 24 (November 23, 2022): 4507-4525.e18. https://doi.org/10.1016/j.cell.2022.10.019.
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  - Pagán, Antonio J., Lauren J. Lee, Joy Edwards-Hicks, Cecilia B. Moens, David M. Tobin, Elisabeth M. Busch-Nentwich, Erika L. Pearce, and Lalita Ramakrishnan. “mTOR-regulated mitochondrial metabolism limits mycobacterium-induced cytotoxicity.” Cell 185, no. 20 (September 29, 2022): 3720-3738.e13. https://doi.org/10.1016/j.cell.2022.08.018.
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  - Tobin, David M. “Modelling infectious disease to support human health.” Dis Model Mech 15, no. 8 (August 1, 2022). https://doi.org/10.1242/dmm.049824.
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  - Hughes, Erika J., and David M. Tobin. “Decoding the tuberculous granuloma.” Immunity 55, no. 5 (May 10, 2022): 819–21. https://doi.org/10.1016/j.immuni.2022.04.009.
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  - Kam, Julia Y., Tina Cheng, Danielle C. Garland, Warwick J. Britton, David M. Tobin, and Stefan H. Oehlers. “Inhibition of infection-induced vascular permeability modulates host leukocyte recruitment to Mycobacterium marinum granulomas in zebrafish.” Pathog Dis 80, no. 1 (April 29, 2022). https://doi.org/10.1093/femspd/ftac009.
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  - Pyle, Charlie J., and David M. Tobin. “Consequential drug combinations for tuberculosis treatments.” Cell Syst 12, no. 11 (November 17, 2021): 1021–22. https://doi.org/10.1016/j.cels.2021.10.005.
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  - Whitworth, L. J., R. Troll, A. J. Pagán, F. J. Roca, P. H. Edelstein, M. Troll, D. M. Tobin, et al. “Erratum: Elevated cerebrospinal fluid cytokine levels in tuberculous meningitis predict survival in response to dexamethasone (Proc. Natl. Acad. Sci. U.S.A. (2021) 118 (e2024852118) DOI: 10.1073/pnas.2024852118).” Proceedings of the National Academy of Sciences of the United States of America 118, no. 41 (October 12, 2021). https://doi.org/10.1073/pnas.2116316118.
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  - Turner, Nicholas A., Mollie I. Sweeney, Ana M. Xet-Mull, Jeremy Storm, Suhail K. Mithani, David B. Jones, Jeremy J. Miles, David M. Tobin, and Jason E. Stout. “A Cluster of Nontuberculous Mycobacterial Tenosynovitis Following Hurricane Relief Efforts.” Clin Infect Dis 72, no. 12 (June 15, 2021): e931–37. https://doi.org/10.1093/cid/ciaa1665.
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  - Wang, Yueyang, Alan Y. Hsu, Eric M. Walton, Sung Jun Park, Ramizah Syahirah, Tianqi Wang, Wenqing Zhou, et al. “A robust and flexible CRISPR/Cas9-based system for neutrophil-specific gene inactivation in zebrafish.” J Cell Sci 134, no. 8 (April 15, 2021). https://doi.org/10.1242/jcs.258574.
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  - Cronan, Mark R., Erika J. Hughes, W Jared Brewer, Gopinath Viswanathan, Emily G. Hunt, Bindu Singh, Smriti Mehra, et al. “A non-canonical type 2 immune response coordinates tuberculous granuloma formation and epithelialization.” Cell 184, no. 7 (April 1, 2021): 1757-1774.e14. https://doi.org/10.1016/j.cell.2021.02.046.
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  - Whitworth, Laura J., Rajan Troll, Antonio J. Pagán, Francisco J. Roca, Paul H. Edelstein, Mark Troll, David M. Tobin, et al. “Elevated cerebrospinal fluid cytokine levels in tuberculous meningitis predict survival in response to dexamethasone.” Proc Natl Acad Sci U S A 118, no. 10 (March 9, 2021). https://doi.org/10.1073/pnas.2024852118.
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  - Ordonez, Alvaro A., Elizabeth W. Tucker, Carolyn J. Anderson, Claire L. Carter, Shashank Ganatra, Deepak Kaushal, Igor Kramnik, et al. “Visualizing the dynamics of tuberculosis pathology using molecular imaging.” J Clin Invest 131, no. 5 (March 1, 2021). https://doi.org/10.1172/JCI145107.
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  - Kam, Julia, Tina Cheng, Danielle Garland, Warwick Britton, David Tobin, and Stefan Oehlers. “Inhibition of infection-induced vascular permeability modulates host leukocyte recruitment to Mycobacterium marinum granulomas in zebrafish,” 2021. https://doi.org/10.1101/2021.11.23.469781.
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  - Viswanathan, Gopinath, and David M. Tobin. “Macrophage ACKRobatics: An atypical Cxcr3 keeps macrophages in check.” J Leukoc Biol 107, no. 2 (February 2020): 171–73. https://doi.org/10.1002/JLB.4CE1019-384R.
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  - McClean, Colleen M., and David M. Tobin. “Early cell-autonomous accumulation of neutral lipids during infection promotes mycobacterial growth.” Plos One 15, no. 5 (2020): e0232251. https://doi.org/10.1371/journal.pone.0232251.
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  - Wang, Yueyang, Alan Hsu, Eric Walton, Ramizah Syahirah, Tianqi Wang, Wenqing Zhou, Chang Ding, Abby Pei Lemke, David Tobin, and Qing Deng. “A CRISPR/Cas9 vector system for neutrophil-specific gene disruption in zebrafish,” 2020. https://doi.org/10.1101/2020.07.27.223008.
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  - Whitworth, Laura, Rajan Troll, Antonio Pagán, Francisco Roca, Paul Edelstein, Mark Troll, David Tobin, et al. “Elevated cerebrospinal fluid cytokine levels in tuberculous meningitis predict survival in response to dexamethasone,” 2020. https://doi.org/10.1101/2020.11.23.394437.
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  - Espenschied, Scott T., Mark R. Cronan, Molly A. Matty, Olaf Mueller, Matthew R. Redinbo, David M. Tobin, and John F. Rawls. “Epithelial delamination is protective during pharmaceutical-induced enteropathy.” Proc Natl Acad Sci U S A 116, no. 34 (August 20, 2019): 16961–70. https://doi.org/10.1073/pnas.1902596116.
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  - Hortle, Elinor, Khelsey E. Johnson, Matt D. Johansen, Tuong Nguyen, Jordan A. Shavit, Warwick J. Britton, David M. Tobin, and Stefan H. Oehlers. “Thrombocyte Inhibition Restores Protective Immunity to Mycobacterial Infection in Zebrafish.” J Infect Dis 220, no. 3 (July 2, 2019): 524–34. https://doi.org/10.1093/infdis/jiz110.
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  - Cronan, Mark R., and David M. Tobin. “Endogenous Tagging at the cdh1 Locus for Live Visualization of E-Cadherin Dynamics.” Zebrafish 16, no. 3 (June 2019): 324–25. https://doi.org/10.1089/zeb.2019.1746.
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  - Patton, E Elizabeth, and David M. Tobin. “Spotlight on zebrafish: the next wave of translational research.” Dis Model Mech 12, no. 3 (March 7, 2019). https://doi.org/10.1242/dmm.039370.
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  - Murdoch, Caitlin C., Scott T. Espenschied, Molly A. Matty, Olaf Mueller, David M. Tobin, and John F. Rawls. “Intestinal Serum amyloid A suppresses systemic neutrophil activation and bactericidal activity in response to microbiota colonization.” Plos Pathog 15, no. 3 (March 2019): e1007381. https://doi.org/10.1371/journal.ppat.1007381.
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  - Keshavjee, Salmaan, Farhana Amanullah, Adithya Cattamanchi, Richard Chaisson, Karen M. Dobos, Gregory J. Fox, Howard E. Gendelman, et al. “Moving toward Tuberculosis Elimination. Critical Issues for Research in Diagnostics and Therapeutics for Tuberculosis Infection.” Am J Respir Crit Care Med 199, no. 5 (March 1, 2019): 564–71. https://doi.org/10.1164/rccm.201806-1053PP.
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  - Matty, Molly A., Daphne R. Knudsen, Eric M. Walton, Rebecca W. Beerman, Mark R. Cronan, Charlie J. Pyle, Rafael E. Hernandez, and David M. Tobin. “Potentiation of P2RX7 as a host-directed strategy for control of mycobacterial infection.” Elife 8 (January 29, 2019). https://doi.org/10.7554/eLife.39123.
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  - Saelens, Joseph W., Gopinath Viswanathan, and David M. Tobin. “Mycobacterial Evolution Intersects With Host Tolerance.” Front Immunol 10 (2019): 528. https://doi.org/10.3389/fimmu.2019.00528.
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  - Cronan, Mark R., Molly A. Matty, Allison F. Rosenberg, Landry Blanc, Charlie J. Pyle, Scott T. Espenschied, John F. Rawls, Véronique Dartois, and David M. Tobin. “An explant technique for high-resolution imaging and manipulation of mycobacterial granulomas.” Nat Methods 15, no. 12 (December 2018): 1098–1107. https://doi.org/10.1038/s41592-018-0215-8.
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  - Walton, Eric M., Mark R. Cronan, C. J. Cambier, Andrea Rossi, Michele Marass, Matthew D. Foglia, W Jared Brewer, et al. “Cyclopropane Modification of Trehalose Dimycolate Drives Granuloma Angiogenesis and Mycobacterial Growth through Vegf Signaling.” Cell Host Microbe 24, no. 4 (October 10, 2018): 514-525.e6. https://doi.org/10.1016/j.chom.2018.09.004.
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  - Murdoch, Caitlin, Scott Espenschied, Molly Matty, Olaf Mueller, David Tobin, and John Rawls. “Intestinal Serum Amyloid A suppresses systemic neutrophil activation and bactericidal activity in response to microbiota colonization,” October 4, 2018. https://doi.org/10.1101/435503.
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  - Jain, Sanjay K., David M. Tobin, Elizabeth W. Tucker, Vishwanath Venketaraman, Alvaro A. Ordonez, Lakshmi Jayashankar, Omar K. Siddiqi, et al. “Tuberculous meningitis: a roadmap for advancing basic and translational research.” Nat Immunol 19, no. 6 (June 2018): 521–25. https://doi.org/10.1038/s41590-018-0119-x.
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  - Saelens, Joseph W., Dalia Lau-Bonilla, Anneliese Moller, Ana M. Xet-Mull, Narda Medina, Brenda Guzmán, Maylena Calderón, et al. “Annotated Genome Sequences of 16 Lineage 4 Mycobacterium tuberculosis Strains from Guatemala.” Genome Announc 6, no. 7 (February 15, 2018). https://doi.org/10.1128/genomeA.00024-18.
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  - Hortle, Elinor, Khelsey Johnson, Matt Johansen, Tuong Nguyen, Jordan Shavit, Warwick Britton, David Tobin, and Stefan Oehlers. “Thrombocyte inhibition restores protective immunity to mycobacterial infection in zebrafish,” 2018. https://doi.org/10.1101/338111.
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  - Wilkinson, Robert J., Ursula Rohlwink, Usha Kant Misra, Reinout van Crevel, Nguyen Thi Hoang Mai, Kelly E. Dooley, Maxine Caws, et al. “Tuberculous meningitis.” Nat Rev Neurol 13, no. 10 (October 2017): 581–98. https://doi.org/10.1038/nrneurol.2017.120.
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  - Alvarez, Monica I., Luke C. Glover, Peter Luo, Liuyang Wang, Elizabeth Theusch, Stefan H. Oehlers, Eric M. Walton, et al. “Human genetic variation in VAC14 regulates Salmonella invasion and typhoid fever through modulation of cholesterol.” Proc Natl Acad Sci U S A 114, no. 37 (September 12, 2017): E7746–55. https://doi.org/10.1073/pnas.1706070114.
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  - Oehlers, Stefan H., Mark R. Cronan, Rebecca W. Beerman, Matthew G. Johnson, Jianhua Huang, Christopher D. Kontos, Jason E. Stout, and David M. Tobin. “Infection-Induced Vascular Permeability Aids Mycobacterial Growth.” J Infect Dis 215, no. 5 (March 1, 2017): 813–17. https://doi.org/10.1093/infdis/jiw355.
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  - Sullivan, C., M. A. Matty, D. Jurczyszak, K. A. Gabor, P. J. Millard, D. M. Tobin, and C. H. Kim. “Infectious disease models in zebrafish.” Methods Cell Biol 138 (2017): 101–36. https://doi.org/10.1016/bs.mcb.2016.10.005.
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  - Cronan, Mark R., Rebecca W. Beerman, Allison F. Rosenberg, Joseph W. Saelens, Matthew G. Johnson, Stefan H. Oehlers, Dana M. Sisk, et al. “Macrophage Epithelial Reprogramming Underlies Mycobacterial Granuloma Formation and Promotes Infection.” Immunity 45, no. 4 (October 18, 2016): 861–76. https://doi.org/10.1016/j.immuni.2016.09.014.
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  - McClean, Colleen M., and David M. Tobin. “Macrophage form, function, and phenotype in mycobacterial infection: lessons from tuberculosis and other diseases.” Pathog Dis 74, no. 7 (October 2016). https://doi.org/10.1093/femspd/ftw068.
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  - Matty, Molly A., Rebecca W. Beerman, and David M. Tobin. “Drug-Inducible, Cell-Specific Manipulation of Intracellular Calcium in Zebrafish Through Mammalian TRPV1 Expression.” Zebrafish 13, no. 4 (August 2016): 374–75. https://doi.org/10.1089/zeb.2016.29004.mat.
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  - Berg, Russell D., Steven Levitte, Mary P. O’Sullivan, Seónadh M. O’Leary, C. J. Cambier, James Cameron, Kevin K. Takaki, et al. “Lysosomal Disorders Drive Susceptibility to Tuberculosis by Compromising Macrophage Migration.” Cell 165, no. 1 (March 24, 2016): 139–52. https://doi.org/10.1016/j.cell.2016.02.034.
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  - Matty, Molly A., Stefan H. Oehlers, and David M. Tobin. “Live Imaging of Host-Pathogen Interactions in Zebrafish Larvae.” Methods Mol Biol 1451 (2016): 207–23. https://doi.org/10.1007/978-1-4939-3771-4_14.
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  - Beerman, Rebecca W., Molly A. Matty, Gina G. Au, Loren L. Looger, Kingshuk Roy Choudhury, Philipp J. Keller, and David M. Tobin. “Direct In Vivo Manipulation and Imaging of Calcium Transients in Neutrophils Identify a Critical Role for Leading-Edge Calcium Flux.” Cell Rep 13, no. 10 (December 15, 2015): 2107–17. https://doi.org/10.1016/j.celrep.2015.11.010.
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  - Cronan, Mark R., Allison F. Rosenberg, Stefan H. Oehlers, Joseph W. Saelens, Dana M. Sisk, Kristen L. Jurcic Smith, Sunhee Lee, and David M. Tobin. “CLARITY and PACT-based imaging of adult zebrafish and mouse for whole-animal analysis of infections.” Dis Model Mech 8, no. 12 (December 2015): 1643–50. https://doi.org/10.1242/dmm.021394.
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  - Saelens, Joseph W., Dalia Lau-Bonilla, Anneliese Moller, Narda Medina, Brenda Guzmán, Maylena Calderón, Raúl Herrera, et al. “Whole genome sequencing identifies circulating Beijing-lineage Mycobacterium tuberculosis strains in Guatemala and an associated urban outbreak.” Tuberculosis (Edinb) 95, no. 6 (December 2015): 810–16. https://doi.org/10.1016/j.tube.2015.09.001.
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  - Tenor, Jennifer L., Stefan H. Oehlers, Jialu L. Yang, David M. Tobin, and John R. Perfect. “Live Imaging of Host-Parasite Interactions in a Zebrafish Infection Model Reveals Cryptococcal Determinants of Virulence and Central Nervous System Invasion.” Mbio 6, no. 5 (September 29, 2015): e01425–e01415. https://doi.org/10.1128/mBio.01425-15.
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  - Wang, Liuyang, Stefan H. Oehlers, Scott T. Espenschied, John F. Rawls, David M. Tobin, and Dennis C. Ko. “CPAG: software for leveraging pleiotropy in GWAS to reveal similarity between human traits links plasma fatty acids and intestinal inflammation.” Genome Biol 16, no. 1 (September 15, 2015): 190. https://doi.org/10.1186/s13059-015-0722-1.
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  - Hart, Bryan E., Rose Asrican, So-Yon Lim, Jaimie D. Sixsmith, Regy Lukose, Sommer J. R. Souther, Swati D. G. Rayasam, et al. “Stable Expression of Lentiviral Antigens by Quality-Controlled Recombinant Mycobacterium bovis BCG Vectors.” Clin Vaccine Immunol 22, no. 7 (July 2015): 726–41. https://doi.org/10.1128/CVI.00075-15.
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  - Tobin, David M. “Host-Directed Therapies for Tuberculosis.” Cold Spring Harb Perspect Med 5, no. 10 (May 18, 2015). https://doi.org/10.1101/cshperspect.a021196.
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  - Marjoram, Lindsay, Ashley Alvers, M Elizabeth Deerhake, Jennifer Bagwell, Jamie Mankiewicz, Jordan L. Cocchiaro, Rebecca W. Beerman, et al. “Epigenetic control of intestinal barrier function and inflammation in zebrafish.” Proc Natl Acad Sci U S A 112, no. 9 (March 3, 2015): 2770–75. https://doi.org/10.1073/pnas.1424089112.
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  - Matty, Molly A., Francisco J. Roca, Mark R. Cronan, and David M. Tobin. “Adventures within the speckled band: heterogeneity, angiogenesis, and balanced inflammation in the tuberculous granuloma.” Immunol Rev 264, no. 1 (March 2015): 276–87. https://doi.org/10.1111/imr.12273.
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  - Oehlers, S. H., M. R. Cronan, N. R. Scott, M. I. Thomas, K. S. Okuda, E. M. Walton, R. W. Beerman, P. S. Crosier, and D. M. Tobin. “Interception of host angiogenic signalling limits mycobacterial growth.” Nature 517, no. 7536 (January 29, 2015): 612–15. https://doi.org/10.1038/nature13967.
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  - Oehlers, Stefan H., Mark R. Cronan, Ninecia R. Scott, Monica I. Thomas, Kazuhide S. Okuda, Eric M. Walton, Rebecca W. Beerman, Philip S. Crosier, and David M. Tobin. “Interception of host angiogenic signalling limits mycobacterial growth.” Nature 517, no. 7536 (January 29, 2015): 612–15. https://doi.org/10.1038/nature13967.
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  - Walton, Eric M., Mark R. Cronan, Rebecca W. Beerman, and David M. Tobin. “The Macrophage-Specific Promoter mfap4 Allows Live, Long-Term Analysis of Macrophage Behavior during Mycobacterial Infection in Zebrafish.” Plos One 10, no. 10 (2015): e0138949. https://doi.org/10.1371/journal.pone.0138949.
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  - Cronan, Mark R., and David M. Tobin. “Fit for consumption: zebrafish as a model for tuberculosis.” Dis Model Mech 7, no. 7 (July 2014): 777–84. https://doi.org/10.1242/dmm.016089.
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  - Whipps, Christopher M., Larry G. Moss, Dana M. Sisk, Katrina N. Murray, David M. Tobin, and Jennifer B. Moss. “Detection of autofluorescent Mycobacterium chelonae in living zebrafish.” Zebrafish 11, no. 1 (February 2014): 76–82. https://doi.org/10.1089/zeb.2012.0863.
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  - Cambier, C. J., Kevin K. Takaki, Ryan P. Larson, Rafael E. Hernandez, David M. Tobin, Kevin B. Urdahl, Christine L. Cosma, and Lalita Ramakrishnan. “Mycobacteria manipulate macrophage recruitment through coordinated use of membrane lipids.” Nature 505, no. 7482 (January 9, 2014): 218–22. https://doi.org/10.1038/nature12799.
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  - Cambier, C. J., K. K. Takaki, R. P. Larson, R. E. Hernandez, D. M. Tobin, K. B. Urdahl, C. L. Cosma, and L. Ramakrishnan. “Mycobacteria manipulate macrophage recruitment through coordinated use of membrane lipids.” Nature 505, no. 7482 (2014): 218–22. https://doi.org/10.1038/nature12799.
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  - Furuse, Yuki, Ryan Finethy, Hector A. Saka, Ana M. Xet-Mull, Dana M. Sisk, Kristen L Jurcic Smith, Sunhee Lee, et al. “Search for microRNAs expressed by intracellular bacterial pathogens in infected mammalian cells.” Plos One 9, no. 9 (2014): e106434. https://doi.org/10.1371/journal.pone.0106434.
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  - Tobin, David M., and Lalita Ramakrishnan. “TB: the Yin and Yang of lipid mediators.” Curr Opin Pharmacol 13, no. 4 (August 2013): 641–45. https://doi.org/10.1016/j.coph.2013.06.007.
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  - Tobin, David M., Francisco J. Roca, John P. Ray, Dennis C. Ko, and Lalita Ramakrishnan. “An enzyme that inactivates the inflammatory mediator leukotriene b4 restricts mycobacterial infection.” Plos One 8, no. 7 (2013): e67828. https://doi.org/10.1371/journal.pone.0067828.
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  - Tobin, David M., Francisco J. Roca, Sungwhan F. Oh, Ross McFarland, Thad W. Vickery, John P. Ray, Dennis C. Ko, et al. “Host genotype-specific therapies can optimize the inflammatory response to mycobacterial infections.” Cell 148, no. 3 (February 3, 2012): 434–46. https://doi.org/10.1016/j.cell.2011.12.023.
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  - Tobin, David M., Robin C. May, and Robert T. Wheeler. “Zebrafish: a see-through host and a fluorescent toolbox to probe host-pathogen interaction.” Plos Pathog 8, no. 1 (January 2012): e1002349. https://doi.org/10.1371/journal.ppat.1002349.
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  - Tobin, David M., Jay C. Vary, John P. Ray, Gregory S. Walsh, Sarah J. Dunstan, Nguyen D. Bang, Deanna A. Hagge, et al. “The lta4h locus modulates susceptibility to mycobacterial infection in zebrafish and humans.” Cell 140, no. 5 (March 5, 2010): 717–30. https://doi.org/10.1016/j.cell.2010.02.013.
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  - Tobin, David M., and Lalita Ramakrishnan. “Comparative pathogenesis of Mycobacterium marinum and Mycobacterium tuberculosis.” Cell Microbiol 10, no. 5 (May 2008): 1027–39. https://doi.org/10.1111/j.1462-5822.2008.01133.x.
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  - Tobin, David M., and Cornelia I. Bargmann. “Invertebrate nociception: behaviors, neurons and molecules.” J Neurobiol 61, no. 1 (October 2004): 161–74. https://doi.org/10.1002/neu.20082.
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  - Liedtke, Wolfgang, David M. Tobin, Cornelia I. Bargmann, and Jeffrey M. Friedman. “Mammalian TRPV4 (VR-OAC) directs behavioral responses to osmotic and mechanical stimuli in Caenorhabditis elegans.” Proc Natl Acad Sci U S A 100 Suppl 2, no. Suppl 2 (November 25, 2003): 14531–36. https://doi.org/10.1073/pnas.2235619100.
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  - Bono, Mario de, David M. Tobin, M Wayne Davis, Leon Avery, and Cornelia I. Bargmann. “Social feeding in Caenorhabditis elegans is induced by neurons that detect aversive stimuli.” Nature 419, no. 6910 (October 31, 2002): 899–903. https://doi.org/10.1038/nature01169.
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  - Tobin, David M., David M. Madsen, Amanda Kahn-Kirby, Erin L. Peckol, Gary Moulder, Robert Barstead, Andres V. Maricq, and Cornelia I. Bargmann. “Combinatorial expression of TRPV channel proteins defines their sensory functions and subcellular localization in C. elegans neurons.” Neuron 35, no. 2 (July 18, 2002): 307–18. https://doi.org/10.1016/s0896-6273(02)00757-2.
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  - Zallen, J. A., E. L. Peckol, D. M. Tobin, and C. I. Bargmann. “Neuronal cell shape and neurite initiation are regulated by the Ndr kinase SAX-1, a member of the Orb6/COT-1/warts serine/threonine kinase family.” Mol Biol Cell 11, no. 9 (September 2000): 3177–90. https://doi.org/10.1091/mbc.11.9.3177.
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  - Chou, J. H., E. R. Troemel, P. Sengupta, H. A. Colbert, L. Tong, D. M. Tobin, K. Roayaie, J. G. Crump, N. D. Dwyer, and C. I. Bargmann. “Olfactory recognition and discrimination in Caenorhabditis elegans.” Cold Spring Harb Symp Quant Biol 61 (1996): 157–64.
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- Conference Papers
  - Pagan, Antonio J., Steven Levitte, Russell D. Berg, Laura Hernandez, Joseph Zimmerman, David M. Tobin, and Lalita Ramakrishnan. “mTOR deficiency reveals an immunological trade-off in innate resistance to mycobacterial infection in vivo.” In Journal of Immunology, Vol. 196. AMER ASSOC IMMUNOLOGISTS, 2016.
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  - Bahr, Nathan C., Suzaan Marais, Maxine Caws, Reinout van Crevel, Robert J. Wilkinson, Jaya S. Tyagi, Guy E. Thwaites, David R. Boulware, and David R. Tuberculous Meningitis International Research Consortium. “GeneXpert MTB/Rif to Diagnose Tuberculous Meningitis: Perhaps the First Test but not the Last.” In Clin Infect Dis, 62:1133–35, 2016. https://doi.org/10.1093/cid/ciw083.
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- Preprints
  - Nicholson, Kathleen R., Rachel M. Cronin, Aruna R. Menon, Madeleine K. Jennisch, David M. Tobin, and Patricia A. Champion. “The EspN transcription factor is an infection-dependent regulator of the ESX-1 system in M. marinum.,” February 16, 2023. https://doi.org/10.1101/2023.02.15.528779.
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  - Pagán, Antonio, Lauren Lee, Joy Edwards-Hicks, Cecilia Moens, David Tobin, Elisabeth Busch-Nentwich, Erika Pearce, and Lalita Ramakrishnan. “mTOR-regulated Mitochondrial Metabolism Limits Mycobacterium-induced Cytotoxicity.” BioRxiv, 2022. https://doi.org/10.1101/2022.01.30.478369.
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Teaching & Mentoring
Recent Courses
Scholarly, Clinical, & Service Activities
Service to Duke
- Executive Committee of Graduate Faculty. September 2017 - 2019 2017 - 2019

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Global Scholarship

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