Scholars@Duke

• Background
• 

  Education, Training, & Certifications

  • Ph.D., Louisiana State University 1999
• 

  Duke Appointment History

  • Assistant Professor of Surgery, Surgery, Surgical Sciences, Surgery 2006 - 2012
  • Assistant Research Professor in Surgery, Surgery, Cardiovascular and Thoracic Surgery, Surgery 2005 - 2006
  • Instructor, Temporary in the Department of Surgery, Surgery, Cardiovascular and Thoracic Surgery, Surgery 2004 - 2005
• Research
• 

  Selected Grants

  • Proteomic Signatures of Space Radiation Induced Cardiovascular Degeneration awarded by National Aeronautics and Space Administration 2016 - 2020
  • Utilization of Two Heartmate III Pumps as a Total Artificial Heart Replacement awarded by St. Jude Medical Inc. 2017 - 2018
  • Protection from cardiac ischemic injury through use of KP544 awarded by Krenitsky Pharmaceuticals Inc. 2017 - 2018
  • A Prospective Pilot Study to Examine the Involvement of apoE Gene Variants in Intracranial Hemorrhage in LVAD Patients awarded by HeartWare, Inc 2015 - 2016
  • E3 Ligases and Deubiquitinases in GPCR Down Regulation awarded by National Institutes of Health 2005 - 2016
  • Heart Risk Model awarded by National Aeronautics and Space Administration 2013 - 2016
  • Expanding the Donor Pool for Heart Transplantation awarded by National Institutes of Health 2012 - 2014
  • Proteomic Profiling of Human Heart Tissue Exposed to Microgravity awarded by National Aeronautics and Space Administration 2012 - 2013
  • Peripheral endothelial and muscle cell pathology in cardiovascular disease awarded by National Institutes of Health 2010 - 2013
  • Autologous EPC lining to improve biocompatibility of circulatory assist devices awarded by National Institutes of Health 2009 - 2012
  • PTEN Gene Therapy for Vein Graft Disease awarded by National Institutes of Health 2009 - 2012
  • Myocardial Gene Transfer during Cardiac Surgery in Swine awarded by National Institutes of Health 2003 - 2008
• Publications & Artistic Works
• 

  Selected Publications

  • Conference Papers

    • Schechter, M, Southerland, K, Feger, B, Njoroge, L, Linder, D, Bowles, D, and Milano, C. "Functional and Biochemical Assessment of Porcine Hearts After Simulated Donation After Circulatory Death." April 2015.  Full Text
    • Schechter, M, Southerland, K, Watson, M, Feger, B, Mishra, R, Schroder, J, Dibernardo, L, Kuchibhatla, M, Daneshmand, M, Patel, C, Rogers, J, Milano, C, and Bowles, D. "Cardiac Troponin I Levels in Preservation Solution Predict Primary Graft Dysfunction After Heart Transplant." April 2015.  Full Text
    • III, PV, Thompson, JW, Soderblom, EJ, Foster, M, Burns, VA, Rogers, JG, Milano, CA, Moseley, MA, and Bowles, DE. "Ischemic Human Heart Failure is Associated With Decreased Phosphorylation of Pyruvate Dehydrogenase and Triosephosphate Isomerase: Proteomic Evidence Supporting Carbohydrate Utilization Preference in the End-Stage Failing Ischemic Heart." November 23, 2010.  Link to Item

  • Journal Articles

    • Jean-Charles, P-Y, Yu, SM-W, Abraham, D, Kommaddi, RP, Mao, L, Strachan, RT, Zhang, Z-S, Bowles, DE, Brian, L, Stiber, JA, Jones, SN, Koch, WJ, Rockman, HA, and Shenoy, SK. "Mdm2 regulates cardiac contractility by inhibiting GRK2-mediated desensitization of β-adrenergic receptor signaling." JCI insight 2, no. 17 (September 7, 2017).  Full Text
    • Wang, T, O'Brien, EC, Rogers, JG, Jacoby, DL, Chen, ME, Testani, JM, Bowles, DE, Milano, CA, Felker, GM, Patel, CB, Bonde, PN, and Ahmad, T. "Plasma Levels of MicroRNA-155 Are Upregulated with Long-Term Left Ventricular Assist Device Support." ASAIO journal (American Society for Artificial Internal Organs : 1992) 63, no. 5 (September 2017): 536-541.  Full Text
    • Santacruz, L, Arciniegas, AJL, Darrabie, M, Mantilla, JG, Baron, RM, Bowles, DE, Mishra, R, and Jacobs, DO. "Hypoxia decreases creatine uptake in cardiomyocytes, while creatine supplementation enhances HIF activation." Physiological reports 5, no. 16 (August 2017).  Full Text
    • Jensen, BC, Bultman, SJ, Holley, D, Tang, W, de Ridder, G, Pizzo, S, Bowles, D, and Willis, MS. "Upregulation of autophagy genes and the unfolded protein response in human heart failure." International Journal of Clinical and Experimental Medicine 10, no. 1 (January 30, 2017): 1051-1058.
    • Guo, H, Mi, Z, Bowles, DE, Bhattacharya, SD, and Kuo, PC. "Osteopontin and protein kinase C regulate PDLIM2 activation and STAT1 ubiquitination in LPS-treated murine macrophages." Journal of Biological Chemistry 292, no. 3 (January 20, 2017): 1142-1142.  Full Text
    • Feger, BJ, Thompson, JW, Dubois, LG, Kommaddi, RP, Foster, MW, Mishra, R, Shenoy, SK, Shibata, Y, Kidane, YH, Moseley, MA, Carnell, LS, and Bowles, DE. "Microgravity induces proteomics changes involved in endoplasmic reticulum stress and mitochondrial protection." Scientific reports 6 (September 27, 2016): 34091-.  Full Text
    • Schechter, MA, Watson, MJ, Feger, BJ, Southerland, KW, Mishra, R, Dibernardo, LR, Kuchibhatla, M, Schroder, JN, Daneshmand, MA, Patel, CB, Rogers, JG, Milano, CA, and Bowles, DE. "Elevated Cardiac Troponin I in Preservation Solution Is Associated With Primary Graft Dysfunction." Journal of cardiac failure 22, no. 2 (February 2016): 158-162.  Full Text
    • Ahmad, T, Kelly, JP, McGarrah, RW, Hellkamp, AS, Fiuzat, M, Testani, JM, Wang, TS, Verma, A, Samsky, MD, Donahue, MP, Ilkayeva, OR, Bowles, DE, Patel, CB, Milano, CA, Rogers, JG, Felker, GM, O'Connor, CM, Shah, SH, and Kraus, WE. "Prognostic Implications of Long-Chain Acylcarnitines in Heart Failure and Reversibility With Mechanical Circulatory Support." Journal of the American College of Cardiology 67, no. 3 (January 2016): 291-299.  Full Text
    • Gaval-Cruz, M, Goertz, RB, Puttick, DJ, Bowles, DE, Meyer, RC, Hall, RA, Ko, D, Paladini, CA, and Weinshenker, D. "Chronic loss of noradrenergic tone produces β-arrestin2-mediated cocaine hypersensitivity and alters cellular D2 responses in the nucleus accumbens." Addiction biology 21, no. 1 (January 2016): 35-48.  Full Text
    • Pratico, ED, Feger, BJ, Watson, MJ, Sullenger, BA, Bowles, DE, Milano, CA, and Nair, SK. "RNA-Mediated Reprogramming of Primary Adult Human Dermal Fibroblasts into c-kit(+) Cardiac Progenitor Cells." Stem cells and development 24, no. 22 (November 2015): 2622-2633.  Full Text
    • Ahmad, T, Wang, T, O'Brien, EC, Samsky, MD, Pura, JA, Lokhnygina, Y, Rogers, JG, Hernandez, AF, Craig, D, Bowles, DE, Milano, CA, Shah, SH, Januzzi, JL, Felker, GM, and Patel, CB. "Effects of left ventricular assist device support on biomarkers of cardiovascular stress, fibrosis, fluid homeostasis, inflammation, and renal injury." JACC. Heart failure 3, no. 1 (January 2015): 30-39.  Full Text
    • Schechter, MA, Hsieh, MKH, Njoroge, LW, Thompson, JW, Soderblom, EJ, Feger, BJ, Troupes, CD, Hershberger, KA, Ilkayeva, OR, Nagel, WL, Landinez, GP, Shah, KM, Burns, VA, Santacruz, L, Hirschey, MD, Foster, MW, Milano, CA, Moseley, MA, Piacentino, V, and Bowles, DE. "Phosphoproteomic profiling of human myocardial tissues distinguishes ischemic from non-ischemic end stage heart failure." PLoS ONE 9, no. 8 (January 2014): e104157-.  Full Text  Open Access Copy
    • Schechter, MA, Southerland, KW, Feger, BJ, Linder, D, Ali, AA, Njoroge, L, Milano, CA, and Bowles, DE. "An isolated working heart system for large animal models." Journal of visualized experiments : JoVE , no. 88 (January 2014).  Full Text  Open Access Copy
    • Foster, MW, Thompson, JW, Forrester, MT, Sha, Y, McMahon, TJ, Bowles, DE, Moseley, MA, and Marshall, HE. "Proteomic analysis of the NOS2 interactome in human airway epithelial cells." Nitric oxide : biology and chemistry 34 (November 2013): 37-46.  Full Text
    • Southerland, KW, Frazier, SB, Bowles, DE, Milano, CA, and Kontos, CD. "Gene therapy for the prevention of vein graft disease." Transl Res 161, no. 4 (April 2013): 321-338. (Review)  Full Text  Link to Item
    • Messina, EL, Nienaber, J, Daneshmand, M, Villamizar, N, Samulski, J, Milano, C, and Bowles, DE. "Adeno-associated viral vectors based on serotype 3b use components of the fibroblast growth factor receptor signaling complex for efficient transduction." Hum Gene Ther 23, no. 10 (October 2012): 1031-1042.  Full Text  Link to Item
    • Piacentino, V, Milano, CA, Bolanos, M, Schroder, J, Messina, E, Cockrell, AS, Jones, E, Krol, A, Bursac, N, Mao, L, Devi, GR, Samulski, RJ, and Bowles, DE. "X-linked inhibitor of apoptosis protein-mediated attenuation of apoptosis, using a novel cardiac-enhanced adeno-associated viral vector." Hum Gene Ther 23, no. 6 (June 2012): 635-646.  Full Text  Link to Item
    • Bowles, DE, McPhee, SWJ, Li, C, Gray, SJ, Samulski, JJ, Camp, AS, Li, J, Wang, B, Monahan, PE, Rabinowitz, JE, Grieger, JC, Govindasamy, L, Agbandje-McKenna, M, Xiao, X, and Samulski, RJ. "Phase 1 gene therapy for Duchenne muscular dystrophy using a translational optimized AAV vector." Mol Ther 20, no. 2 (February 2012): 443-455.  Full Text  Link to Item
    • Li, C, Diprimio, N, Bowles, DE, Hirsch, ML, Monahan, PE, Asokan, A, Rabinowitz, J, Agbandje-McKenna, M, and Samulski, RJ. "Single amino acid modification of adeno-associated virus capsid changes transduction and humoral immune profiles." Journal of Virology 86, no. 15 (2012): 7752-7759.  Full Text
    • Sun, Q-A, Hess, DT, Nogueira, L, Yong, S, Bowles, DE, Eu, J, Laurita, KR, Meissner, G, and Stamler, JS. "Oxygen-coupled redox regulation of the skeletal muscle ryanodine receptor-Ca2+ release channel by NADPH oxidase 4." Proc Natl Acad Sci U S A 108, no. 38 (September 20, 2011): 16098-16103.  Full Text  Link to Item
    • Darrabie, MD, Arciniegas, AJL, Mishra, R, Bowles, DE, Jacobs, DO, and Santacruz, L. "AMPK and substrate availability regulate creatine transport in cultured cardiomyocytes." Am J Physiol Endocrinol Metab 300, no. 5 (May 2011): E870-E876.  Full Text  Link to Item
    • Villamizar, NR, Crow, JH, Piacentino, V, DiBernardo, LR, Daneshmand, MA, Bowles, DE, Groh, MA, and Milano, CA. "Reproducibility of left atrial ablation with high-intensity focused ultrasound energy in a calf model." J Thorac Cardiovasc Surg 140, no. 6 (December 2010): 1381-7.e1.  Full Text  Link to Item
    • Guo, H, Mi, Z, Bowles, DE, Bhattacharya, SD, and Kuo, PC. "Osteopontin and protein kinase C regulate PDLIM2 activation and STAT1 ubiquitination in LPS-treated murine macrophages." J Biol Chem 285, no. 48 (November 26, 2010): 37787-37796.  Full Text  Link to Item
    • Chen, E, Everett, ML, Holzknecht, ZE, Holzknecht, RA, Lin, SS, Bowles, DE, and Parker, W. "Short-lived alpha-helical intermediates in the folding of beta-sheet proteins." Biochemistry 49, no. 26 (July 6, 2010): 5609-5619.  Full Text  Open Access Copy  Link to Item
    • Lee, SM, Wyse, A, Lesher, A, Everett, ML, Lou, L, Holzknecht, ZE, Whitesides, JF, Spears, PA, Bowles, DE, Lin, SS, Tonkonogy, SL, Orndorff, PE, Bollinger, RR, and Parker, W. "Adaptation in a mouse colony monoassociated with Escherichia coli K-12 for more than 1,000 days." Appl Environ Microbiol 76, no. 14 (July 2010): 4655-4663.  Full Text  Link to Item
    • Crow, S, Chen, D, Milano, C, Thomas, W, Joyce, L, III, VP, Sharma, R, Wu, J, Arepally, G, Bowles, D, Rogers, J, and Villamizar-Ortiz, N. "Acquired von Willebrand syndrome in continuous-flow ventricular assist device recipients." Annals of Thoracic Surgery 90, no. 4 (2010): 1263-1269.  Full Text
    • Crow, S, Milano, C, Joyce, L, Chen, D, Arepally, G, Bowles, D, Thomas, W, and Ortiz, NV. "Comparative analysis of von willebrand factor profiles in pulsatile and continuous left ventricular assist device recipients." ASAIO Journal 56, no. 5 (2010): 441-445.  Full Text
    • Mendell, JR, Campbell, K, Rodino-Klapac, L, Sahenk, Z, Shilling, C, Lewis, S, Bowles, D, Gray, S, Li, C, Galloway, G, Malik, V, Coley, B, Clark, KR, Li, J, Xiao, X, Samulski, J, McPhee, SW, Samulski, RJ, and Walker, CM. "Dystrophin immunity in Duchenne's muscular dystrophy." New England Journal of Medicine 363, no. 15 (2010): 1429-1437.  Full Text
    • Petersen, TH, Hitchcock, T, Muto, A, Calle, EA, Zhao, L, Gong, Z, Gui, L, Dardik, A, Bowles, DE, Counter, CM, and Niklason, LE. "Utility of telomerase-pot1 fusion protein in vascular tissue engineering." Cell Transplant 19, no. 1 (2010): 79-87.  Full Text  Open Access Copy  Link to Item
    • Petersen, TH, Hitchcock, T, Muto, A, Calle, EA, Zhao, L, Gong, Z, Gui, L, Dardik, A, Bowles, DE, Counter, CM, and Niklason, LE. "Utility of telomerase-pot1 fusion protein in vascular tissue engineering." Cell transplantation 19, no. 1 (2010): 79-87.  Full Text
    • Lima, B, Lam, GKW, Xie, L, Diesen, DL, Villamizar, N, Nienaber, J, Messina, E, Bowles, D, Kontos, CD, Hare, JM, Stamler, JS, and Rockman, HA. "Endogenous S-nitrosothiols protect against myocardial injury." Proc Natl Acad Sci U S A 106, no. 15 (April 14, 2009): 6297-6302.  Full Text  Link to Item
    • Li, C, Bowles, DE, Dyke, TV, and Samulski, RJ. "Adeno-associated virus vectors: Potential applications for cancer gene therapy." Cancer Gene Therapy 12, no. 12 (2005): 913-925.  Full Text
    • Rabinowitz, JE, Bowles, DE, Faust, SM, Ledford, JG, Cunningham, SE, and Samulski, RJ. "Cross-Dressing the Virion: The Transcapsidation of Adeno- Associated Virus Serotypes Functionally Defines Subgroups." Journal of Virology 78, no. 9 (2004): 4421-4432.  Full Text
    • Bowles, DE, Rabinowitz, JE, and Samulski, RJ. "Marker rescue of adeno-associated virus (AAV) capsid mutants: A novel approach for chimeric AAV production." Journal of Virology 77, no. 1 (2003): 423-432.  Full Text
    • Bowles, DE, Kim, SK, and O'Callaghan, DJ. "Characterization of the trans-activation properties of equine herpesvirus 1 EICP0 protein." Journal of Virology 74, no. 3 (2000): 1200-1208.  Full Text
    • Kim, SK, Bowles, DE, and O'Callaghan, DJ. "The γ2 late glycoprotein K promoter of equine herpesvirus 1 is differentially regulated by the IE and EICP0 proteins." Virology 256, no. 2 (1999): 173-179.  Full Text
    • Bowles, DE, Holden, VR, Zhao, Y, and O'Callaghan, DJ. "The ICP0 protein of equine herpesvirus 1 is an early protein that independently transactivates expression of all classes of viral promoters." Journal of Virology 71, no. 7 (1997): 4904-4914.

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