Scholars@Duke

• Background
• 

  Education, Training, & Certifications

  • Ph.D., Louisiana State University 1999
• 

  Duke Appointment History

  • Assistant Professor of Surgery, Surgery, Surgical Sciences, Surgery 2006 - 2012
  • Assistant Research Professor in Surgery, Surgery, Cardiovascular and Thoracic Surgery, Surgery 2005 - 2006
  • Instructor, Temporary in the Department of Surgery, Surgery, Cardiovascular and Thoracic Surgery, Surgery 2004 - 2005
• Research
• 

  Selected Grants

  • Evolving Inside the Box awarded by North Carolina Biotechnology Center 2018 - 2020
  • Proteomic Signatures of Space Radiation Induced Cardiovascular Degeneration awarded by National Aeronautics and Space Administration 2016 - 2020
  • Utilization of Two Heartmate III Pumps as a Total Artificial Heart Replacement awarded by St. Jude Medical Inc. 2017 - 2019
  • Expanding the capabilities and the utilization of the Duke Human Heart Repository awarded by North Carolina Biotechnology Center 2017 - 2019
  • Cadaver Implant Studies of PABP awarded by 3R Life Sciences Taiwan Ltd. 2018
  • Protection from cardiac ischemic injury through use of KP544 awarded by Krenitsky Pharmaceuticals Inc. 2017 - 2018
  • A Prospective Pilot Study to Examine the Involvement of apoE Gene Variants in Intracranial Hemorrhage in LVAD Patients awarded by HeartWare, Inc 2015 - 2016
  • E3 Ligases and Deubiquitinases in GPCR Down Regulation awarded by National Institutes of Health 2005 - 2016
  • Heart Risk Model awarded by National Aeronautics and Space Administration 2013 - 2016
  • Expanding the Donor Pool for Heart Transplantation awarded by National Institutes of Health 2012 - 2014
  • Proteomic Profiling of Human Heart Tissue Exposed to Microgravity awarded by National Aeronautics and Space Administration 2012 - 2013
  • Peripheral endothelial and muscle cell pathology in cardiovascular disease awarded by National Institutes of Health 2010 - 2013
  • Autologous EPC lining to improve biocompatibility of circulatory assist devices awarded by National Institutes of Health 2009 - 2012
  • PTEN Gene Therapy for Vein Graft Disease awarded by National Institutes of Health 2009 - 2012
  • Myocardial Gene Transfer during Cardiac Surgery in Swine awarded by National Institutes of Health 2003 - 2008
• Publications & Artistic Works
• 

  Selected Publications

  • Academic Articles

    • Ren, Linda, Robert A. Holzknecht, Zoie E. Holzknecht, Sanet H. Kotzé, Dawn E. Bowles, Shu S. Lin, Erin A. McKenney, and William Parker. “A mole rat's gut microbiota suggests selective influence of diet on microbial niche space and evolution..” Exp Biol Med (Maywood) 244, no. 6 (April 2019): 471–83. https://doi.org/10.1177/1535370219828703.
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    • Beak, JY, Kang, HS, Huang, W, Myers, PH, Bowles, DE, Jetten, AM, and Jensen, BC. "The nuclear receptor RORα protects against angiotensin II-induced cardiac hypertrophy and heart failure." American Journal of Physiology. Heart and Circulatory Physiology 316, no. 1 (January 2019): H186-H200.  Full Text
    • Stevenson, Mark D., Chandrika Canugovi, Aleksandr E. Vendrov, Takayuki Hayami, Dawn E. Bowles, Karl-Heinz Krause, Nageswara R. Madamanchi, and Marschall S. Runge. “NADPH Oxidase 4 Regulates Inflammation in Ischemic Heart Failure: Role of Soluble Epoxide Hydrolase..” Antioxidants & Redox Signaling, December 28, 2018. https://doi.org/10.1089/ars.2018.7548.
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    • Bishawi, Muath, Jeremy Joseph, Chetan Patel, Jacob Schroder, Mani Daneshmand, Dawn Bowles, Joseph Rogers, and Carmelo Milano. “Risk factors for stroke on left ventricular assist devices..” Journal of Cardiac Surgery 33, no. 6 (June 2018): 348–52. https://doi.org/10.1111/jocs.13718.
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    • Bishawi, Muath, Jun-Neng Roan, Jordan Richards, Zachary Brown, Laura Blue, Mani A. Daneshmand, Jacob N. Schroder, Dawn E. Bowles, and Carmelo A. Milano. “Use of Two Intracorporeal Ventricular Assist Devices As a Total Artificial Heart..” Journal of Visualized Experiments : Jove, no. 135 (May 11, 2018). https://doi.org/10.3791/55961.
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    • Hayashi, Hiroki, Douglas T. Hess, Rongli Zhang, Keiki Sugi, Huiyun Gao, Bea L. Tan, Dawn E. Bowles, et al. “S-Nitrosylation of β-Arrestins Biases Receptor Signaling and Confers Ligand Independence..” Mol Cell 70, no. 3 (May 3, 2018): 473-487.e6. https://doi.org/10.1016/j.molcel.2018.03.034.
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    • Jean-Charles, Pierre-Yves, Samuel Mon-Wei Yu, Dennis Abraham, Reddy Peera Kommaddi, Lan Mao, Ryan T. Strachan, Zhu-Shan Zhang, et al. “Mdm2 regulates cardiac contractility by inhibiting GRK2-mediated desensitization of β-adrenergic receptor signaling..” Jci Insight 2, no. 17 (September 7, 2017). https://doi.org/10.1172/jci.insight.95998.
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    • Wang, Teresa, Emily C. O’Brien, Joseph G. Rogers, Daniel L. Jacoby, Michael E. Chen, Jeffrey M. Testani, Dawn E. Bowles, et al. “Plasma Levels of MicroRNA-155 Are Upregulated with Long-Term Left Ventricular Assist Device Support..” Asaio Journal (American Society for Artificial Internal Organs : 1992) 63, no. 5 (September 2017): 536–41. https://doi.org/10.1097/MAT.0000000000000564.
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    • Santacruz, Lucia, Antonio Jose Luis Arciniegas, Marcus Darrabie, Jose G. Mantilla, Rebecca M. Baron, Dawn E. Bowles, Rajashree Mishra, and Danny O. Jacobs. “Hypoxia decreases creatine uptake in cardiomyocytes, while creatine supplementation enhances HIF activation..” Physiological Reports 5, no. 16 (August 2017). https://doi.org/10.14814/phy2.13382.
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    • Jensen, B. C., S. J. Bultman, D. Holley, W. Tang, G. de Ridder, S. Pizzo, D. Bowles, and M. S. Willis. “Upregulation of autophagy genes and the unfolded protein response in human heart failure.” International Journal of Clinical and Experimental Medicine 10, no. 1 (January 30, 2017): 1051–58.
    • Guo, H., Z. Mi, D. E. Bowles, S. D. Bhattacharya, and P. C. Kuo. “Erratum: Osteopontin and protein kinase C regulate PDLIM2 activation and STAT1 ubiquitination in LPS-treated murine macrophages (The Journal of Biological Chemistry (2017) 292:3 (1142) DOI: 10.1074/jbc.M110.161869).” Journal of Biological Chemistry 292, no. 3 (January 20, 2017). https://doi.org/10.1074/jbc.A110.161869.
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    • Feger, Bryan J., J Will Thompson, Laura G. Dubois, Reddy P. Kommaddi, Matthew W. Foster, Rajashree Mishra, Sudha K. Shenoy, et al. “Microgravity induces proteomics changes involved in endoplasmic reticulum stress and mitochondrial protection..” Scientific Reports 6 (September 27, 2016). https://doi.org/10.1038/srep34091.
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    • Schechter, Matthew A., Michael J. Watson, Bryan J. Feger, Kevin W. Southerland, Rajashree Mishra, Louis R. Dibernardo, Maragatha Kuchibhatla, et al. “Elevated Cardiac Troponin I in Preservation Solution Is Associated With Primary Graft Dysfunction..” Journal of Cardiac Failure 22, no. 2 (February 2016): 158–62. https://doi.org/10.1016/j.cardfail.2015.08.339.
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    • Ahmad, Tariq, Jacob P. Kelly, Robert W. McGarrah, Anne S. Hellkamp, Mona Fiuzat, Jeffrey M. Testani, Teresa S. Wang, et al. “Prognostic Implications of Long-Chain Acylcarnitines in Heart Failure and Reversibility With Mechanical Circulatory Support..” Journal of the American College of Cardiology 67, no. 3 (January 2016): 291–99. https://doi.org/10.1016/j.jacc.2015.10.079.
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    • Gaval-Cruz, Meriem, Richard B. Goertz, Daniel J. Puttick, Dawn E. Bowles, Rebecca C. Meyer, Randy A. Hall, Daijin Ko, Carlos A. Paladini, and David Weinshenker. “Chronic loss of noradrenergic tone produces β-arrestin2-mediated cocaine hypersensitivity and alters cellular D2 responses in the nucleus accumbens..” Addiction Biology 21, no. 1 (January 2016): 35–48. https://doi.org/10.1111/adb.12174.
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    • Pratico, Elizabeth D., Bryan J. Feger, Michael J. Watson, Bruce A. Sullenger, Dawn E. Bowles, Carmelo A. Milano, and Smita K. Nair. “RNA-Mediated Reprogramming of Primary Adult Human Dermal Fibroblasts into c-kit(+) Cardiac Progenitor Cells..” Stem Cells Dev 24, no. 22 (November 15, 2015): 2622–33. https://doi.org/10.1089/scd.2015.0073.
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    • Ahmad, Tariq, Teresa Wang, Emily C. O’Brien, Marc D. Samsky, John A. Pura, Yuliya Lokhnygina, Joseph G. Rogers, et al. “Effects of left ventricular assist device support on biomarkers of cardiovascular stress, fibrosis, fluid homeostasis, inflammation, and renal injury..” Jacc Heart Fail 3, no. 1 (January 2015): 30–39. https://doi.org/10.1016/j.jchf.2014.06.013.
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    • Schechter, Matthew A., Kevin W. Southerland, Bryan J. Feger, Dean Linder, Ayyaz A. Ali, Linda Njoroge, Carmelo A. Milano, and Dawn E. Bowles. “An isolated working heart system for large animal models..” Journal of Visualized Experiments : Jove, no. 88 (January 2014). https://doi.org/10.3791/51671.
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    • Schechter, Matthew A., Michael K. H. Hsieh, Linda W. Njoroge, J Will Thompson, Erik J. Soderblom, Bryan J. Feger, Constantine D. Troupes, et al. “Phosphoproteomic profiling of human myocardial tissues distinguishes ischemic from non-ischemic end stage heart failure..” Plos One 9, no. 8 (2014). https://doi.org/10.1371/journal.pone.0104157.
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    • Foster, Matthew W., J Will Thompson, Michael T. Forrester, Yonggang Sha, Timothy J. McMahon, Dawn E. Bowles, M Arthur Moseley, and Harvey E. Marshall. “Proteomic analysis of the NOS2 interactome in human airway epithelial cells..” Nitric Oxide 34 (November 1, 2013): 37–46. https://doi.org/10.1016/j.niox.2013.02.079.
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    • Southerland, Kevin W., Sarah B. Frazier, Dawn E. Bowles, Carmelo A. Milano, and Christopher D. Kontos. “Gene therapy for the prevention of vein graft disease..” Translational Research : The Journal of Laboratory and Clinical Medicine 161, no. 4 (April 2013): 321–38. https://doi.org/10.1016/j.trsl.2012.12.003.
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    • Messina, Emily L., Jeffrey Nienaber, Mani Daneshmand, Nestor Villamizar, Jude Samulski, Carmelo Milano, and Dawn E. Bowles. “Adeno-associated viral vectors based on serotype 3b use components of the fibroblast growth factor receptor signaling complex for efficient transduction..” Human Gene Therapy 23, no. 10 (October 2012): 1031–42. https://doi.org/10.1089/hum.2012.066.
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    • Li, Chengwen, Nina Diprimio, Dawn E. Bowles, Matthew L. Hirsch, Paul E. Monahan, Aravind Asokan, Joseph Rabinowitz, Mavis Agbandje-McKenna, and R Jude Samulski. “Single amino acid modification of adeno-associated virus capsid changes transduction and humoral immune profiles..” Journal of Virology 86, no. 15 (August 2012): 7752–59. https://doi.org/10.1128/JVI.00675-12.
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    • Piacentino, Valentino, Carmelo A. Milano, Michael Bolanos, Jacob Schroder, Emily Messina, Adam S. Cockrell, Edward Jones, et al. “X-linked inhibitor of apoptosis protein-mediated attenuation of apoptosis, using a novel cardiac-enhanced adeno-associated viral vector..” Human Gene Therapy 23, no. 6 (June 2012): 635–46. https://doi.org/10.1089/hum.2011.186.
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    • Bowles, Dawn E., Scott W. J. McPhee, Chengwen Li, Steven J. Gray, Jade J. Samulski, Angelique S. Camp, Juan Li, et al. “Phase 1 gene therapy for Duchenne muscular dystrophy using a translational optimized AAV vector..” Mol Ther 20, no. 2 (February 2012): 443–55. https://doi.org/10.1038/mt.2011.237.
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    • Sun, Qi-An, Douglas T. Hess, Leonardo Nogueira, Sandro Yong, Dawn E. Bowles, Jerry Eu, Kenneth R. Laurita, Gerhard Meissner, and Jonathan S. Stamler. “Oxygen-coupled redox regulation of the skeletal muscle ryanodine receptor-Ca2+ release channel by NADPH oxidase 4..” Proceedings of the National Academy of Sciences of the United States of America 108, no. 38 (September 6, 2011): 16098–103. https://doi.org/10.1073/pnas.1109546108.
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    • Darrabie, Marcus D., Antonio Jose Luis Arciniegas, Rajashree Mishra, Dawn E. Bowles, Danny O. Jacobs, and Lucia Santacruz. “AMPK and substrate availability regulate creatine transport in cultured cardiomyocytes..” American Journal of Physiology. Endocrinology and Metabolism 300, no. 5 (May 2011): E870–76. https://doi.org/10.1152/ajpendo.00554.2010.
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    • Villamizar, Nestor R., Jennifer H. Crow, Valentino Piacentino, Louis R. DiBernardo, Mani A. Daneshmand, Dawn E. Bowles, Mark A. Groh, and Carmelo A. Milano. “Reproducibility of left atrial ablation with high-intensity focused ultrasound energy in a calf model..” The Journal of Thoracic and Cardiovascular Surgery 140, no. 6 (December 2010): 1381-7.e1. https://doi.org/10.1016/j.jtcvs.2010.08.029.
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    • Guo, Hongtao, Zhiyong Mi, Dawn E. Bowles, Syamal D. Bhattacharya, and Paul C. Kuo. “Osteopontin and protein kinase C regulate PDLIM2 activation and STAT1 ubiquitination in LPS-treated murine macrophages..” The Journal of Biological Chemistry 285, no. 48 (November 2010): 37787–96. https://doi.org/10.1074/jbc.M110.161869.
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    • Crow, Sheri, Dong Chen, Carmelo Milano, William Thomas, Lyle Joyce, Valentino Piacentino, Riti Sharma, et al. “Acquired von Willebrand syndrome in continuous-flow ventricular assist device recipients..” The Annals of Thoracic Surgery 90, no. 4 (October 2010): 1263–69. https://doi.org/10.1016/j.athoracsur.2010.04.099.
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    • Mendell, Jerry R., Katherine Campbell, Louise Rodino-Klapac, Zarife Sahenk, Chris Shilling, Sarah Lewis, Dawn Bowles, et al. “Dystrophin immunity in Duchenne's muscular dystrophy..” The New England Journal of Medicine 363, no. 15 (October 2010): 1429–37. https://doi.org/10.1056/NEJMoa1000228.
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    • Crow, Sheri, Carmelo Milano, Lyle Joyce, Dong Chen, Gowthami Arepally, Dawn Bowles, William Thomas, and Nestor Villamizar Ortiz. “Comparative analysis of von Willebrand factor profiles in pulsatile and continuous left ventricular assist device recipients..” Asaio Journal (American Society for Artificial Internal Organs : 1992) 56, no. 5 (September 2010): 441–45. https://doi.org/10.1097/mat.0b013e3181e5de0a.
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    • Chen, Eefei, Mary Lou Everett, Zoie E. Holzknecht, Robert A. Holzknecht, Shu S. Lin, Dawn E. Bowles, and William Parker. “Short-lived alpha-helical intermediates in the folding of beta-sheet proteins..” Biochemistry 49, no. 26 (July 2010): 5609–19. https://doi.org/10.1021/bi100288q.
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    • Lee, Sean M., Aaron Wyse, Aaron Lesher, Mary Lou Everett, Linda Lou, Zoie E. Holzknecht, John F. Whitesides, et al. “Adaptation in a mouse colony monoassociated with Escherichia coli K-12 for more than 1,000 days..” Applied and Environmental Microbiology 76, no. 14 (July 2010): 4655–63. https://doi.org/10.1128/AEM.00358-10.
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    • Petersen, T. H., T. Hitchcock, A. Muto, E. A. Calle, L. Zhao, Z. Gong, L. Gui, et al. “Utility of telomerase-pot1 fusion protein in vascular tissue engineering..” Cell Transplantation 19, no. 1 (2010): 79–87. https://doi.org/10.3727/096368909X478650.
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    • Petersen, Thomas H., Thomas Hitchcock, Akihito Muto, Elizabeth A. Calle, Liping Zhao, Zhaodi Gong, Liqiong Gui, et al. “Utility of telomerase-pot1 fusion protein in vascular tissue engineering..” Cell Transplantation 19, no. 1 (January 2010): 79–87. https://doi.org/10.3727/096368909X478650.
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    • Lima, Brian, Gregory K. W. Lam, Liang Xie, Diana L. Diesen, Nestor Villamizar, Jeffrey Nienaber, Emily Messina, et al. “Endogenous S-nitrosothiols protect against myocardial injury..” Proc Natl Acad Sci U S A 106, no. 15 (April 14, 2009): 6297–6302. https://doi.org/10.1073/pnas.0901043106.
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    • Li, Chengwen, Dawn E. Bowles, Terry van Dyke, and Richard Jude Samulski. “Adeno-associated virus vectors: potential applications for cancer gene therapy..” Cancer Gene Therapy 12, no. 12 (December 2005): 913–25. https://doi.org/10.1038/sj.cgt.7700876.
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    • Rabinowitz, Joseph E., Dawn E. Bowles, Susan M. Faust, Julie G. Ledford, Scott E. Cunningham, and R Jude Samulski. “Cross-dressing the virion: the transcapsidation of adeno-associated virus serotypes functionally defines subgroups..” Journal of Virology 78, no. 9 (May 2004): 4421–32. https://doi.org/10.1128/JVI.78.9.4421-4432.2004.
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    • Bowles, Dawn E., Joseph E. Rabinowitz, and R Jude Samulski. “Marker rescue of adeno-associated virus (AAV) capsid mutants: a novel approach for chimeric AAV production..” Journal of Virology 77, no. 1 (January 2003): 423–32. https://doi.org/10.1128/JVI.77.1.423-432.2003.
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    • Bowles, D. E., S. K. Kim, and D. J. O’Callaghan. “Characterization of the trans-activation properties of equine herpesvirus 1 EICP0 protein..” Journal of Virology 74, no. 3 (February 2000): 1200–1208. https://doi.org/10.1128/jvi.74.3.1200-1208.2000.
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    • Kim, S. K., D. E. Bowles, and D. J. O’callaghan. “The gamma2 late glycoprotein K promoter of equine herpesvirus 1 is differentially regulated by the IE and EICP0 proteins..” Virology 256, no. 2 (April 10, 1999): 173–79. https://doi.org/10.1006/viro.1999.9608.
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    • Bowles, D. E., V. R. Holden, Y. Zhao, and D. J. O’Callaghan. “The ICP0 protein of equine herpesvirus 1 is an early protein that independently transactivates expression of all classes of viral promoters..” Journal of Virology 71, no. 7 (July 1997): 4904–14.

  • Conference Papers

    • Bishawi, Muath, Jun-Neng Roan, Carmelo A. Milano, Mani A. Daneshmand, Jacob N. Schroder, Zachary D. Brown, Adam Nevo, et al. “Wide Spread Adenoviral Vector Delivery to a Cardiac Allograft Utilizing an Ex Vivo Perfusion Storage Strategy.” In Molecular Therapy, 26:69–69. CELL PRESS, 2018.
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    • Schechter, M. A., K. W. Southerland, B. J. Feger, L. Njoroge, D. Linder, D. E. Bowles, and C. A. Milano. “Functional and Biochemical Assessment of Porcine Hearts After Simulated Donation After Circulatory Death.” In The Journal of Heart and Lung Transplantation, 34:S150–S150. Elsevier BV, 2015. https://doi.org/10.1016/j.healun.2015.01.403.
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    • Schechter, M. A., K. W. Southerland, M. J. Watson, B. J. Feger, R. Mishra, J. N. Schroder, L. R. Dibernardo, et al. “Cardiac Troponin I Levels in Preservation Solution Predict Primary Graft Dysfunction After Heart Transplant.” In The Journal of Heart and Lung Transplantation, 34:S268–S268. Elsevier BV, 2015. https://doi.org/10.1016/j.healun.2015.01.750.
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    • Piacentino III, P., J. W. Thompson, E. J. Soderblom, M. Foster, V. A. Burns, J. G. Rogers, C. A. Milano, M. A. Moseley, and D. E. Bowles. “Ischemic Human Heart Failure is Associated With Decreased Phosphorylation of Pyruvate Dehydrogenase and Triosephosphate Isomerase: Proteomic Evidence Supporting Carbohydrate Utilization Preference in the End-Stage Failing Ischemic Heart.” In Circulation, Vol. 122, 2010.
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