Scholars@Duke
David E. Hinton

David E. Hinton

Nicholas Distinguished Professor Emeritus of Environmental Quality
Environmental Natural Science
dhinton@duke.edu
Box 90328, Durham, NC 27708-0328
A333A LSRC, Durham, NC 27708

Scholarly Works - Journal articles

The role of chorion integrity on the bioaccumulation and toxicity of selenium nanoparticles in Japanese medaka (Oryzias latipes).

Journal article Aquatic toxicology (Amsterdam, Netherlands) · January 2025 Selenium nanoparticles (nano-Se) have a wide range of biomedical and agricultural applications. However, there is little information on the potential toxicity of nano-Se once it enters the environment, particularly in fish. The first line of defense from c ... Full text Cite

Morphologic alterations across three levels of biological organization following oral exposure to silver-polymer nanocomposites in Japanese medaka (Oryzias latipes)

Journal article Environmental Science Nano · July 3, 2024 Polymer nanocomposites have diverse industrial and commercial uses. While many toxicity studies have assessed the individual materials (e.g., polymer, nanomaterial) comprising nanocomposites, few have examined the potential toxicity of the nanocomposite as ... Full text Cite

Single and mixture toxicity of cadmium and copper to swim bladder in early life stages of Japanese medaka (Oryzias latipes).

Journal article Environmental geochemistry and health · January 2024 Toxicity observed in aquatic ecosystems often cannot be explained by the action of a single pollutant. Likewise, evaluation standards formulated by a single effect cannot truly reflect the environmental quality requirements. The study of mixtures is needed ... Full text Cite

Impacts of ingested MWCNT-Embedded nanocomposites in Japanese medaka (Oryzias latipes).

Journal article Nanotoxicology · December 2021 Polymer nanocomposites combine the versatile, lightweight characteristics of polymers with the properties of nanomaterials. Polyethylene terephthalate glycol (PETG) is commonly used in polymer additive manufacturing due to its controllable transparency, hi ... Full text Cite

Pre-validation of choriogenin H transgenic medaka eleutheroembryos as a quantitative estrogenic activity test method.

Journal article Analytical biochemistry · September 2021 The choriogenin H - EGFP transgenic medaka (Oryzias melastigma) has been used to test estrogenic substances and quantify estrogenic activity into 17β-estradiol (E2) equivalency (EEQ). The method uses 8 eleutheroembryos in 2 ml solution per well and 3 wells ... Full text Cite

Microplastics decrease the toxicity of triphenyl phosphate (TPhP) in the marine medaka (Oryzias melastigma) larvae.

Journal article The Science of the total environment · April 2021 Plastics have been recognized as a serious threat to the environment. Besides their own toxicity, microplastics can interact with other environmental pollutants, acting as carriers and potentially modulating their toxicity. In this study, the toxicity of p ... Full text Cite

Heart development in two populations of Atlantic killifish (Fundulus heteroclitus) following exposure to a polycyclic aromatic hydrocarbon mixture.

Journal article Ecotoxicology and environmental safety · January 2021 Historic industrial pollution of the Elizabeth River, Virginia resulted in polycyclic aromatic hydrocarbon (PAH) contamination in sediments. Atlantic killifish (Fundulus heteroclitus) inhabiting the Atlantic Wood (AW) industrial site adapted to complex PAH ... Full text Cite

Developmental toxicity from exposure to various forms of mercury compounds in medaka fish (Oryzias latipes) embryos

Journal article Peerj · January 1, 2021 This study examined developmental toxicity of different mercury compounds, including some used in traditional medicines. Medaka (Oryzias latipes) embryos were exposed to 0.001-10 μM concentrations of MeHg, HgCl2, α-HgS (Zhu Sha), and β-HgS (Zuotai) from st ... Full text Cite

PAH-pollution effects on sensitive and resistant embryos: Integrating structure and function with gene expression.

Journal article PloS one · January 2021 Polycyclic aromatic hydrocarbons (PAHs) are among the most widespread natural and anthropogenic pollutants, and some PAHs are proven developmental toxicants. We chemically characterized clean and heavily polluted sites and exposed fish embryos to PAH pollu ... Full text Cite

Selenomethionine exposure affects chondrogenic differentiation and bone formation in Japanese medaka (Oryzias latipes).

Journal article Journal of hazardous materials · April 2020 Excess selenium entering the aquatic environment from anthropogenic activities has been associated with developmental abnormalities in fish including skeletal deformities of the head and spine. However, mechanisms of this developmental toxicity have not be ... Full text Cite

Chronic dietary exposure to polystyrene microplastics in maturing Japanese medaka (Oryzias latipes).

Journal article Aquatic toxicology (Amsterdam, Netherlands) · March 2020 Fish studies report consumption of microplastics (MPs) in the field, and concern exists over associated risks. However, laboratory studies with adult fish are scarce. In this study, outbred and see-through Japanese medaka (Oryzias latipes) were fed diets a ... Full text Cite

Chronic microfiber exposure in adult Japanese medaka (Oryzias latipes).

Journal article PloS one · January 2020 Microplastic fibers (MFs) pollute aquatic habitats globally via sewage release, stormwater runoff, or atmospheric deposition. Of the synthetic MFs, polyester (PES) and polypropylene (PP) are the most common. Field studies show that fish ingest large quanti ... Full text Open Access Cite

Pathology in Ecological Research With Implications for One Health: Session Summary.

Journal article Toxicologic pathology · December 2019 This session explored the effects of pollutants on One Health at the ecosystem level that included microbes, insects, fish, and humans. The concept of One Health seeks to synergize medical, veterinary, and other health science disciplines to more effective ... Full text Cite

HBx-K130M/V131I Promotes Liver Cancer in Transgenic Mice via AKT/FOXO1 Signaling Pathway and Arachidonic Acid Metabolism.

Journal article Molecular cancer research : MCR · July 2019 Chronic hepatitis B viral (HBV) infection remains a high underlying cause for hepatocellular carcinoma (HCC) worldwide, while the genetic mechanisms behind this remain unclear. This study elucidated the mechanisms contributing to tumor development induced ... Full text Cite

Use of biological detection methods to assess dioxin-like compounds in sediments of Bohai Bay, China.

Journal article Ecotoxicology and environmental safety · May 2019 Bohai Bay, in the western region of northeastern China's Bohai Sea, receives water from large rivers containing various pollutants including dioxin-like compounds (DLCs). This study used the established zebrafish (Danio rerio) model, its known developmenta ... Full text Open Access Cite

The case for thyroid disruption in early life stage exposures to thiram in zebrafish (Danio rerio).

Journal article General and comparative endocrinology · January 2019 Thiram, a pesticide in the dithiocarbamate chemical family, is widely used to prevent fungal disease in seeds and crops. Its off-site movement to surface waters occurs and may place aquatic organisms at potential harm. Zebrafish embryos were used for inves ... Full text Open Access Cite

Microplastics in Small Waterbodies and Tadpoles from Yangtze River Delta, China.

Journal article Environmental science & technology · August 2018 Although microplastic (MP) pollution in freshwater systems is gaining attention, our knowledge of its distribution in small waterbodies is scarce. Small waterbodies are freshwater habitats to many species, including amphibians, that are vulnerable to MP po ... Full text Cite

Maternal dietary exposure to selenium nanoparticle led to malformation in offspring.

Journal article Ecotoxicology and environmental safety · July 2018 Selenium (Se) is an essential element and its biological activity is related to its speciation. It is also well-known that in excess it can cause teratogenesis in fish and birds. In this study we compared dietary toxicity of elemental selenium nanoparticle ... Full text Cite

Increased coiling frequency linked to apoptosis in the brain and altered thyroid signaling in zebrafish embryos (Danio rerio) exposed to the PBDE metabolite 6-OH-BDE-47.

Journal article Chemosphere · May 2018 Polybrominated diphenyl ethers (PBDEs) are a group of brominated flame retardants that are ubiquitously detected in the environment and associated with adverse health outcomes. 6-OH-BDE-47 is a metabolite of the flame retardant, 2,2',4,4'-Tetrabromodipheny ... Full text Open Access Cite

Later life swimming performance and persistent heart damage following subteratogenic PAH mixture exposure in the Atlantic killifish (Fundulus heteroclitus).

Journal article Environmental toxicology and chemistry · December 2017 High-level, acute exposures to individual polycyclic aromatic hydrocarbons (PAHs) and complex PAH mixtures result in cardiac abnormalities in developing fish embryos. Whereas acute PAH exposures can be developmentally lethal, little is known about the late ... Full text Cite

Early life co-exposures to a real-world PAH mixture and hypoxia result in later life and next generation consequences in medaka (Oryzias latipes).

Journal article Aquatic toxicology (Amsterdam, Netherlands) · September 2017 Acute effects of individual and complex mixtures of polycyclic aromatic hydrocarbons (PAHs) are well documented in vertebrate species. Hypoxia in fish reduces metabolic rate and reproduction. However, less is known about the later life consequences stemmin ... Full text Cite

Exposure to a PBDE/OH-BDE mixture alters juvenile zebrafish (Danio rerio) development.

Journal article Environ Toxicol Chem · January 2017 Polybrominated diphenyl ethers (PBDEs) and their metabolites (e.g., hydroxylated BDEs [OH-BDEs]) are contaminants frequently detected together in human tissues and are structurally similar to thyroid hormones. Thyroid hormones partially mediate metamorphic ... Full text Link to item Cite

Silver nanoparticle toxicity is related to coating materials and disruption of sodium concentration regulation.

Journal article Nanotoxicology · November 2016 Silver nanoparticles (AgNPs) have been increasingly commercialized and their release into the environment is imminent. Toxicity of AgNP has been studied with a wide spectrum of organisms, yet the mechanism of toxicity remains largely unknown. This study sy ... Full text Open Access Cite

Halogenated carbazoles induce cardiotoxicity in developing zebrafish (Danio rerio) embryos.

Journal article Environmental toxicology and chemistry · October 2016 Halogenated carbazoles are increasingly identified as a novel class of environmental contaminants. However, no in vivo acute toxicity information on those compounds was available. In the present study, an in vivo zebrafish embryonic model (Danio rerio) was ... Full text Cite

AHR2 morpholino knockdown reduces the toxicity of total particulate matter to zebrafish embryos.

Journal article Toxicology and applied pharmacology · October 2016 The zebrafish embryo has been proposed as a 'bridge model' to study the effects of cigarette smoke on early development. Previous studies showed that exposure to total particulate matter (TPM) led to adverse effects in developing zebrafish, and suggested t ... Full text Cite

Developmental toxicity from exposure to various forms of mercury compounds in medaka fish (Oryzias latipes) embryos

Journal article PeerJ · August 23, 2016 This study examined developmental toxicity of different mercury compounds, including some used in traditional medicines. Medaka (Oryzias latipes) embryos were exposed to 0.001–10 µM concentrations of MeHg, HgCl2, α-HgS (Zhu Sha), and β-HgS (Zuotai) from st ... Full text Open Access Cite

Hepatic Responses of Juvenile Fundulus heteroclitus from Pollution-adapted and Nonadapted Populations Exposed to Elizabeth River Sediment Extract.

Journal article Toxicologic pathology · July 2016 Atlantic killifish (Fundulus heteroclitus) inhabiting the Atlantic Wood Industries region of the Elizabeth River, Virginia, have passed polycyclic aromatic hydrocarbon (PAH) resistance to their offspring as evidenced by early life stage testing of developm ... Full text Open Access Cite

Parental dietary seleno-L-methionine exposure and resultant offspring developmental toxicity.

Journal article Aquatic toxicology (Amsterdam, Netherlands) · January 2016 Selenium (Se) leaches into water from agricultural soils and from storage sites for coal fly ash. Se toxicity causes population and community level effects in fishes and birds. We used the laboratory aquarium model fish, Japanese medaka (Oryzias latipes), ... Full text Open Access Cite

Advancing toxicology research using in vivo high throughput toxicology with small fish models.

Journal article ALTEX · January 2016 Small freshwater fish models, especially zebrafish, offer advantages over traditional rodent models, including low maintenance and husbandry costs, high fecundity, genetic diversity, physiology similar to that of traditional biomedical models, and reduced ... Full text Cite

Developmental toxicity of the PBDE metabolite 6-OH-BDE-47 in zebrafish and the potential role of thyroid receptor β.

Journal article Aquatic toxicology (Amsterdam, Netherlands) · November 2015 6-hydroxy-2,2',4,4'-tetrabromodiphenyl ether (6-OH-BDE-47) is both a polybrominated diphenyl ether (PBDE) flame retardant metabolite and a marine natural product. It has been identified both as a neurotoxicant in cell-based studies and as a developmental t ... Full text Cite

Developmental toxicity and DNA damage from exposure to parking lot runoff retention pond samples in the Japanese medaka (Oryzias latipes).

Journal article Marine environmental research · August 2014 Parking lot runoff retention ponds (PLRRP) receive significant chemical input, but the biological effects of parking lot runoff are not well understood. We used the Japanese medaka (Oryzias latipes) as a model to study the toxicity of water and sediment sa ... Full text Cite

Bioaccumulation and speciation of selenium in fish and insects collected from a mountaintop removal coal mining-impacted stream in West Virginia.

Journal article Ecotoxicology (London, England) · July 2014 A major contaminant of concern for mountaintop removal/valley fill (MTR/VF) coal mining is selenium (Se), an essential micronutrient that can be toxic to fish. Creek chubs (Semotilus atromaculatus), green sunfish (Lepomis cyanellus), and composite insect s ... Full text Cite

Silver nanoparticle behavior, uptake, and toxicity in Caenorhabditis elegans: effects of natural organic matter.

Journal article Environmental science & technology · March 2014 Significant progress has been made in understanding the toxicity of silver nanoparticles (Ag NPs) under carefully controlled laboratory conditions. Natural organic matter (NOM) is omnipresent in complex environmental systems, where it may alter the behavio ... Full text Cite

Phenotypic characterization of transgenic Japanese medaka (Oryzias latipes) that express a red fluorescent protein in hepatocytes.

Journal article Toxicologic pathology · January 2014 Transgenic organisms that express fluorescent proteins are used frequently for in vivo visualization of proteins and cells. The phenotype of a transgenic medaka (Oryzias latipes) strain that expresses a red fluorescent protein (RFP) in hepatocytes was char ... Full text Cite

Immunohistochemical characterization of the hepatic progenitor cell compartment in medaka (Oryzias latipes) following hepatic injury.

Journal article Journal of comparative pathology · November 2013 Laboratory fish species are used increasingly in biomedical research and are considered robust models for the study of regenerative processes. Studies investigating the response of the fish liver to injury have demonstrated the presence of a ductular react ... Full text Cite

Anchoring hepatic gene expression with development of fibrosis and neoplasia in a toxicant-induced fish model of liver injury.

Journal article Toxicologic pathology · July 2013 Fish have been used as laboratory models to study hepatic development and carcinogenesis but not for pathogenesis of hepatic fibrosis. In this study, a dimethylnitrosamine-induced fish model of hepatic injury was developed in Japanese medaka (Oryzias latip ... Full text Cite

Using whole mount in situ hybridization to examine thyroid hormone deiodinase expression in embryonic and larval zebrafish: a tool for examining OH-BDE toxicity to early life stages.

Journal article Aquatic toxicology (Amsterdam, Netherlands) · May 2013 Polybrominated diphenyl ethers (PBDEs) and their oxidative metabolites (hydroxylated PBDEs; OH-BDEs) are known endocrine disrupting contaminants that have been shown to disrupt thyroid hormone regulation both in mammals and in fish. The purpose of this stu ... Full text Cite

UVC-induced mitochondrial degradation via autophagy correlates with mtDNA damage removal in primary human fibroblasts.

Journal article Journal of biochemical and molecular toxicology · January 2013 Mitochondrial DNA (mtDNA) is more susceptible than nuclear DNA to helix-distorting damage via exposure to environmental genotoxins, partially due to a lack of nucleotide excision repair. Thus, this damage is irreparable and persistent in mtDNA in the short ... Full text Cite

Uptake of silver nanoparticles and toxicity to early life stages of Japanese medaka (Oryzias latipes): effect of coating materials.

Journal article Aquatic toxicology (Amsterdam, Netherlands) · September 2012 Silver nanoparticles (AgNPs) with antimicrobial properties are perhaps the most deployed engineered nanomaterials in consumer products. Almost all AgNPs are coated with organic materials to enhance their dispersion in water. Contributions of coatings to th ... Full text Cite

A high-throughput histoarray for quantitative molecular profiling of multiple, uniformly oriented medaka (Oryzias latipes) embryos.

Journal article Comparative biochemistry and physiology. Toxicology & pharmacology : CBP · January 2012 Embryos of aquatic animal model fish have proven to be useful organisms for developmental biology and for early life stage toxicity tests. By virtue of their transparent chorions, imaging of normal and abnormal development can be detected and related to ex ... Full text Cite

Aquatic models, genomics and chemical risk management.

Journal article Comparative biochemistry and physiology. Toxicology & pharmacology : CBP · January 2012 The 5th Aquatic Animal Models for Human Disease meeting follows four previous meetings (Nairn et al., 2001; Schmale, 2004; Schmale et al., 2007; Hinton et al., 2009) in which advances in aquatic animal models for human disease research were reported, and c ... Full text Cite

TCDD disrupts hypural skeletogenesis during medaka embryonic development.

Journal article Toxicological sciences : an official journal of the Society of Toxicology · January 2012 Defective bone and cartilage development account for a large number of human birth defects annually. Normal skeletogenesis involves cartilage development in early morphogenesis through a highly coordinated and orchestrated series of events involving commit ... Full text Cite

Anchoring ethinylestradiol induced gene expression changes with testicular morphology and reproductive function in the medaka.

Journal article PloS one · January 2012 Environmental estrogens are ubiquitous in the environment and can cause detrimental effects on male reproduction. In fish, a multitude of effects from environmental estrogens have been observed including altered courting behavior and fertility, sex reversa ... Full text Cite

Generation and characterization of neurogenin1-GFP transgenic medaka with potential for rapid developmental neurotoxicity screening.

Journal article Aquatic toxicology (Amsterdam, Netherlands) · September 2011 Fish models such as zebrafish and medaka are increasingly used as alternatives to rodents in developmental and toxicological studies. These developmental and toxicological studies can be facilitated by the use of transgenic reporters that permit the real-t ... Full text Cite

Accumulation and debromination of decabromodiphenyl ether (BDE-209) in juvenile fathead minnows (Pimephales promelas) induces thyroid disruption and liver alterations.

Journal article Toxicological sciences : an official journal of the Society of Toxicology · August 2011 Polybrominated diphenyl ether (PBDE) flame retardants are known to affect thyroid hormone (TH) regulation. The TH-regulating deiodinases have been implicated in these impacts; however, PBDE effects on the fish thyroid system are largely unknown. Moreover, ... Full text Cite

Gene expression throughout a vertebrate's embryogenesis.

Journal article BMC genomics · February 2011 BackgroundDescribing the patterns of gene expression during embryonic development has broadened our understanding of the processes and patterns that define morphogenesis. Yet gene expression patterns have not been described throughout vertebrate e ... Full text Cite

A simple osmium post-fixation paraffin-embedment technique to identify lipid accumulation in fish liver using medaka (Oryziaslatipes) eggs and eleutheroembryos as lipid rich models.

Journal article Marine pollution bulletin · January 2011 Hepatic lipidosis is a non-specific biomarker of effect from pollution exposure in fish. Fatty liver is often misdiagnosed or overlooked in histological assessments due to the decreasing application of specific fat procedures and stains. For example, ethan ... Full text Cite

Comparative chronic liver toxicity of benzo[a]pyrene in two populations of the atlantic killifish (Fundulus heteroclitus) with different exposure histories.

Journal article Environmental health perspectives · October 2010 BackgroundThe Atlantic Wood Industries Superfund site on the Elizabeth River (ER) in Portsmouth, Virginia, is contaminated with polycyclic aromatic hydrocarbons (PAHs) derived from creosote. Embryos and larvae of ER killifish (Fundulus heteroclitu ... Full text Cite

Long-term trends in liver neoplasms in brown bullhead in the Buffalo River, New York, USA.

Journal article Environmental toxicology and chemistry · August 2010 The Buffalo River area of concern (AOC) was assigned an impaired status for the fish tumors and other deformities beneficial use impairment category by the New York State Department of Environmental Protection in 1989. This was initially based on an inadeq ... Full text Cite

The fish embryo toxicity test as an animal alternative method in hazard and risk assessment and scientific research.

Journal article Aquatic toxicology (Amsterdam, Netherlands) · April 2010 Animal alternatives research has historically focused on human safety assessments and has only recently been extended to environmental testing. This is particularly for those assays that involve the use of fish. A number of alternatives are being pursued b ... Full text Cite

Exposure to the synthetic FXR agonist GW4064 causes alterations in gene expression and sublethal hepatotoxicity in eleutheroembryo medaka (Oryzias latipes).

Journal article Toxicology and applied pharmacology · February 2010 The small freshwater teleost, medaka (Oryzias latipes), has a history of usage in studies of chronic toxicity of liver and biliary system. Recent progress with this model has focused on defining the medaka hepatobiliary system. Here we investigate critical ... Full text Cite

Aquatic animal models of human disease: selected papers and recommendations from the 4th Conference.

Journal article Comparative biochemistry and physiology. Toxicology & pharmacology : CBP · March 2009 Full text Cite

Use of medaka in toxicity testing.

Journal article Current protocols in toxicology · February 2009 Small aquarium fishes are increasingly used as animal models, and one of these, the Japanese Medaka (Oryzias latipes), is frequently utilized for toxicity testing. While these vertebrates have many similarities with their terrestrial counterparts, there ar ... Full text Cite

Embryo toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin to the wood duck (Aix sponsa).

Journal article Archives of environmental contamination and toxicology · November 2008 We examined the sensitivity of the wood duck (Aix sponsa) embryo to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) by injecting the toxicant into their eggs. Six groups of wood duck eggs (n = 35 to 211 per trial) were injected with 0 to 4600 pg TCDD/g egg betw ... Full text Cite

Non invasive in vivo investigation of hepatobiliary structure and function in STII medaka (Oryzias latipes): methodology and applications.

Journal article Comparative hepatology · October 2008 BackgroundA novel transparent stock of medaka (Oryzias latipes; STII), recessive for all pigments found in chromatophores, permits transcutaneous imaging of internal organs and tissues in living individuals. Findings presented describe the develop ... Full text Cite

Protective response of the Ah receptor to ANIT-induced biliary epithelial cell toxicity in see-through medaka.

Journal article Toxicological sciences : an official journal of the Society of Toxicology · April 2008 The adaptive role of the aryl hydrocarbon receptor (Ah receptor or AHR) in protecting against disease-related conditions remains unclear in nonmammalian models, particularly teleosts. Therefore, this study focused on the potential role of AHR in response t ... Full text Cite

Non invasive high resolution in vivo imaging of alpha-naphthylisothiocyanate (ANIT) induced hepatobiliary toxicity in STII medaka.

Journal article Aquatic toxicology (Amsterdam, Netherlands) · January 2008 A novel transparent stock of medaka (Oryzias latipes; STII), homozygous recessive for all four pigments (iridophores, xanthophores, leucophores, melanophores), permits transcutaneous, high resolution (<1 microm) imaging of internal organs and tissues in li ... Full text Cite

Non invasive in vivo investigation of hepatobiliary structure and function in STII medaka (Oryzias latipes): methodology and applications.

Journal article Comparative hepatology · 2008 BACKGROUND: A novel transparent stock of medaka (Oryzias latipes; STII), recessive for all pigments found in chromatophores, permits transcutaneous imaging of internal organs and tissues in living individuals. Findings presented describe the development of ... Full text Cite

Biomonitoring recycled water in the Santa Ana River Basin in southern California.

Journal article Journal of toxicology and environmental health. Part A · January 2008 The Santa Ana River (SAR) is the primary source of groundwater recharge for the Orange County Groundwater Basin in coastal southern California. Approximately 85% base flow in the SAR originates from wastewater treatment plants operated by three dischargers ... Full text Cite

An artificial fertilization method with the Japanese medaka: implications in early life stage bioassays and solvent toxicity.

Journal article Ecotoxicology and environmental safety · January 2008 An in vitro fertilization method was used to study the effects on medaka (Oryzias latipes) embryos reared either from 0.5h (early blastodisc) or 6.5h (early blastula) post-fertilization for 200 h in varying concentrations of dimethyl sulfoxide (DMSO), meth ... Full text Cite

Differential developmental toxicity of naphthoic acid isomers in medaka (Oryzias latipes) embryos.

Journal article Marine pollution bulletin · January 2008 Polycyclic aromatic hydrocarbons (PAHs) are widespread persistent pollutants that readily undergo biotic and abiotic conversion to numerous transformation products in rivers, lakes and estuarine sediments. Here we characterize the developmental toxicity of ... Full text Cite

An in vivo look at vertebrate liver architecture: three-dimensional reconstructions from medaka (Oryzias latipes).

Journal article Anatomical record (Hoboken, N.J. : 2007) · July 2007 Understanding three-dimensional (3D) hepatobiliary architecture is fundamental to elucidating structure/function relationships relevant to hepatobiliary metabolism, transport, and toxicity. To date, factual information on vertebrate liver architecture in 3 ... Full text Cite

Comparisons of polychromatic and monochromatic UV-based treatments of bisphenol-A in water via toxicity assessments.

Journal article Chemosphere · June 2007 Polychromatic ultraviolet irradiation, such as from medium pressure (MP) Hg lamps may enhance the UV degradation of environmental pollutants as compared to low pressure (LP) Hg UV sources emitting monochromatic irradiation. Typically, studies involving des ... Full text Cite

Estrogenic modulation of CYP3A38, CYP3A40, and CYP19 in mature male medaka (Oryzias latipes).

Journal article Comparative biochemistry and physiology. Toxicology & pharmacology : CBP · April 2007 We examined cytochrome P450 production and activity and circulating hormone concentrations in male medaka exposed to 17beta-estradiol (E2) or 17alpha-ethinylestradiol (EE2). Intraperitoneal injection of E2 at 1, 10, or 100 microg/g-fish completely suppress ... Full text Cite

Biological assessments of a mixture of endocrine disruptors at environmentally relevant concentrations in water following UV/H2O2 oxidation.

Journal article The Science of the total environment · April 2007 Numerous studies have investigated degradation of individual endocrine disrupting compounds (EDCs) in lab or natural waters. However, natural variations in water matrices and mixtures of EDCs in the environment may confound analysis of the treatment effici ... Full text Cite

Age-dependent in situ hepatic and gill CYP1A activity in the see-through medaka (Oryzias latipes).

Journal article Comparative biochemistry and physiology. Toxicology & pharmacology : CBP · February 2007 We used a recently introduced strain of medaka, the see-through medaka, whose internal organs can be seen through the skin, to develop an in situ toxicity assay of ethoxyresorufin-O-deethylase (EROD) activity that detected fluorescence from resorufin, a me ... Full text Cite

Vulnerable windows for developmental ethanol toxicity in the Japanese medaka fish (Oryzias latipes).

Journal article Aquatic toxicology (Amsterdam, Netherlands) · December 2006 Ethanol (EtOH) is a well-known developmental toxicant that produces a range of abnormal phenotypes in mammalian systems including craniofacial abnormalities, cognitive deficits and growth retardation. While the toxic potential of developmental EtOH exposur ... Full text Cite

Biological assessment of bisphenol A degradation in water following direct photolysis and UV advanced oxidation.

Journal article Chemosphere · November 2006 Endocrine disrupting compounds (EDCs) are exogenous environmental chemicals that can interfere with normal hormone function and present a potential threat to both environmental and human health. The fate, distribution and degradation of EDCs is a subject o ... Full text Cite

Adapting the medaka embryo assay to a high-throughput approach for developmental toxicity testing.

Journal article Neurotoxicology · September 2006 Chemical exposure during embryonic development may cause persistent effects, yet developmental toxicity data exist for very few chemicals. Current testing procedures are time consuming and costly, underlining the need for rapid and low cost screening strat ... Full text Cite

Toxic actions of dinoseb in medaka (Oryzias latipes) embryos as determined by in vivo 31P NMR, HPLC-UV and 1H NMR metabolomics.

Journal article Aquatic toxicology (Amsterdam, Netherlands) · March 2006 Changes in metabolism of Japanese medaka (Oryzias latipes) embryos exposed to dinoseb (2-sec-butyl-4,6-dinitrophenol), a substituted dinitrophenol herbicide, were determined by in vivo (31)P NMR, high-pressure liquid chromatography (HPLC)-UV, and (1)H NMR ... Full text Cite

Dynamic gene expression changes precede dioxin-induced liver pathogenesis in medaka fish.

Journal article Toxicological sciences : an official journal of the Society of Toxicology · February 2006 A major challenge for environmental genomics is linking gene expression to cellular toxicity and morphological alteration. Herein, we address complexities related to hepatic gene expression responses after a single injection of the aryl hydrocarbon recepto ... Full text Cite

Functional characterization of medaka CYP3A38 and CYP3A40: kinetics and catalysis by expression in a recombinant baculovirus system.

Journal article Comparative biochemistry and physiology. Toxicology & pharmacology : CBP · August 2005 Phylogenic analysis of the teleost genomic lineages has demonstrated the precedent for multiple genome duplications. Among many of the genes duplicated, cytochrome P450 genes have undergone independent diversification, which can be traced to a single ances ... Full text Cite

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) induces organ- specific differential gene expression in male Japanese medaka (Oryzias latipes).

Journal article Toxicological sciences : an official journal of the Society of Toxicology · May 2005 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a ubiquitous environmental contaminant with well-known adverse effects in fish. In this study, we initially exploited suppression subtractive hybridization (SSH) as a screening tool to assess qualitative gene e ... Full text Cite

A quick reference guide to the normal development of Oryzias latipes (Teleostei, Adrianichthyidae)

Journal article Journal of Applied Ichthyology · February 1, 2005 Published reports describing the normal embryo development of Oryzias latipes Temminck and Schlegel, 1846, have relied on exhaustive descriptive processes supported by a series of detailed line drawn figures representing the ideal condition for each stage. ... Full text Cite

Resolving mechanisms of toxicity while pursuing ecotoxicological relevance?

Journal article Marine pollution bulletin · January 2005 In this age of modern biology, aquatic toxicological research has pursued mechanisms of action of toxicants. This has provided potential tools for ecotoxicologic investigations. However, problems of biocomplexity and issues at higher levels of biological o ... Full text Cite

Metabolic changes in Japanese medaka (Oryzias latipes) during embryogenesis and hypoxia as determined by in vivo 31P NMR.

Journal article Comparative biochemistry and physiology. Toxicology & pharmacology : CBP · January 2005 In vivo (31)P nuclear magnetic resonance spectroscopy (NMR) was used to determine phosphometabolite changes in medaka (Oryzias latipes) during embryogenesis and hypoxia. NMR data were acquired using a flow-through NMR tube perfusion system designed to both ... Full text Cite

Stage-specific toxicity of cypermethrin to medaka (Oryzias latipes) eggs and embryos using a refined methodology for an in vitro fertilization bioassay.

Journal article Archives of environmental contamination and toxicology · January 2005 Using original artificial fertilization methods with medaka (Oryzias latipes), the effects of exposure to cypermethrin on gametes, fertilization, and embryonic development were investigated. The relative sensitivity was studied with 96-hour duration, 24-ho ... Full text Cite

Does shaking alter the time of hatching in incubated medaka (Oryzias latipes) embryos?

Journal article Bulletin of environmental contamination and toxicology · August 2004 Full text Cite

Analysis of medaka cytochrome P450 3A homotropic and heterotropic cooperativity.

Journal article Marine environmental research · August 2004 We have previously demonstrated that medaka CYP3A is associated with metabolism of several endobiotics including steroids and bile acids. In this study, we demonstrate that medaka CYP3A catalysis exhibits unusual kinetic behaviors consistent with allosteri ... Full text Cite

Biochemical and toxicopathic biomarkers assessed in smallmouth bass recovered from a polychlorinated biphenyl-contaminated river.

Journal article Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals · July 2004 Smallmouth bass (Micropterus dolomieu) were collected to quantify the nature and prevalence of biomarker responses, including biochemical indices, toxicopathic lesions and general health indices, among fish collected from polychlorinated biphenyl (PCB)-con ... Full text Cite

Imaging Liver Development/Remodeling in the See-Through Medaka Fish.

Journal article Comparative hepatology · January 2004 Full text Cite

ras oncogene mutations in diethylnitrosamine-induced hepatic tumors in medaka (Oryzias latipes), a teleost fish.

Journal article Mutation research · August 2003 Medaka fish are an established non-mammalian research model for the study of liver carcinogenesis and exposure to environmental pollutants. Studies have emphasized the development of hepatic neoplasms in medaka following exposure to model carcinogens. To d ... Full text Cite

Influence of water hardening of the chorion on cadmium accumulation in medaka (Oryzias latipes) eggs.

Journal article Chemosphere · July 2003 This report describes a study in which in vitro fertilization methods were used to expose medaka (Oryzias latipes) eggs to cadmium (Cd(2+)). This approach was applied to address the differential sensitivity and cumulative potential of Cd(2+) when exposure ... Full text Cite

Stage sensitivity of medaka (Oryzias latipes) eggs and embryos to permethrin.

Journal article Aquatic toxicology (Amsterdam, Netherlands) · February 2003 The effects of exposure to permethrin on gametes, fertilization and embryonic development were examined in medaka (Oryzias latipes). Following range finding (25, 50, 100, 200 or 300 microg/l) and duration of exposure (0, 120, 144, 168, 192, 216, or 240-h) ... Full text Cite

Joint acute toxicity of esfenvalerate and diazinon to larval fathead minnows (Pimephales promelas).

Journal article Environmental toxicology and chemistry · February 2003 California (USA) agriculture employs pyrethroid and organophosphate insecticides to control insects in orchards and other crops. Diazinon and esfenvalerate were selected for this study because of their application overlaps. Toxicological and biochemical re ... Full text Cite

Effects of husbandry practices, gender, and normal physiological variation on growth and reproduction of Japanese medaka, Oryzias latipes.

Journal article Aquatic toxicology (Amsterdam, Netherlands) · October 2002 Japanese medaka, Oryzias latipes, are currently used in a variety of research applications for toxicological and carcinogenesis research, yet the impact of certain husbandry factors on study outcome has received limited attention. In this study, we demonst ... Full text Cite

Utilizing in vivo nuclear magnetic resonance spectroscopy to study sublethal stress in aquatic organisms.

Journal article Marine environmental research · September 2002 In vivo nuclear magnetic resonance spectroscopy (NMR) is a powerful technique for characterizing the sublethal actions of physical and chemical stressors in live, intact organisms. In particular, 31P NMR is ideal for observing perturbations to cellular ene ... Full text Cite

Effects of dietary exposure to the pyrethroid pesticide esfenvalerate on medaka (Oryzias latipes).

Journal article Marine environmental research · September 2002 The pyrethroid insecticide esfenvalerate is widely used on orchard crops throughout California. In the aquatic environment, this compound is likely to accumulate in sediments, food particles and benthic organisms due to its lipophilicity and environmental ... Full text Cite

Runoff from orchard floors - Micro-plot field experiments and modeling

Journal article Journal of Hydrology · August 30, 2002 Hydrologic response patterns of three ground treatments (resident vegetation, bare soil and ripped resident vegetation) were investigated. An in situ, micro-scale, plot retention-tank system was employed and used in conjunction with a kinematic wave model ... Full text Cite

Molecular cloning of rainbow trout (Oncorhynchus mykiss) eggshell zona radiata protein complementary DNA: mRNA expression in 17beta-estradiol- and nonylphenol-treated fish.

Journal article Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology · June 2002 A complementary DNA (cDNA) encoding the eggshell zona radiata protein (RBTZR: AF407574) has been cloned from the liver of estradiol-17beta (E(2))-treated rainbow trout (Oncorhynchus mykiss) by reverse-transcriptase polymerase chain reaction (RT-PCR). A set ... Full text Cite

Selenium toxicity: cause and effects in aquatic birds.

Journal article Aquatic toxicology (Amsterdam, Netherlands) · April 2002 There are several manners in which selenium may express its toxicity: (1) an important mechanism appears to involve the formation of CH(3)Se(minus sign) which either enters a redox cycle and generates superoxide and oxidative stress, or forms free radicals ... Full text Cite

Effects of handling on heat shock protein expression in rainbow trout (Oncorhynchus mykiss).

Journal article Environmental toxicology and chemistry · March 2002 As part of an effort to validate the use of heat shock proteins (HSPs) as biomarkers of exposure to and effects of contaminants, we evaluated the effect of two handling regimens on the induction of HSP 60 and 70 in rainbow trout (Oncorhynchus mykiss). Fish ... Full text Cite

Runoff from orchard floors

Journal article Journal of Hydrology · 2002 Cite

Preface

Journal article Aquatic Toxicology · January 1, 2002 Full text Cite

Increasing uptake and bioactivation with development positively modulate diazinon toxicity in early life stage medaka (Oryzias latipes).

Journal article Toxicological sciences : an official journal of the Society of Toxicology · June 2001 Diazinon, an organophosphate pesticide, becomes biotransformed to a more potent oxon metabolite that inhibits acetylcholinesterase (AChE). Early life stages (els) of medaka, Oryzias latipes, were used to determine how development of this teleost affects se ... Full text Cite

Differential biomarker gene and protein expressions in nonylphenol and estradiol-17beta treated juvenile rainbow trout (Oncorhynchus mykiss).

Journal article Comparative biochemistry and physiology. Toxicology & pharmacology : CBP · May 2001 The time- and dose-dependent transcriptional and translational expression of biomarker genes in nonylphenol (NP) and estradiol-17beta (E(2)) treated juvenile rainbow trout is reported. Fish were exposed to NP (1, 5 and 25 mg/kg) and E(2) (5 mg/kg) and kill ... Full text Cite

Identification, characterization, and ontogeny of a second cytochrome P450 3A gene from the fresh water teleost medaka (Oryzias latipes).

Journal article Molecular reproduction and development · February 2001 Multiple copies of cytochrome P450 gene family 3 have been identified from numerous mammalian species. Often these genes exhibit differential catalytic activities and gene regulation. To date however, little information is available regarding multiple form ... Full text Cite

Evaluation of fish models of soluble epoxide hydrolase inhibition.

Journal article Environmental health perspectives · January 2001 Substituted ureas and carbamates are mechanistic inhibitors of the soluble epoxide hydrolase (sEH). We screened a set of chemicals containing these functionalities in larval fathead minnow (Pimphales promelas) and embryo/larval golden medaka (Oryzias latip ... Full text Cite

Cytochrome P450 1A- and stress protein-induction in early life stages of medaka (Oryzias latipes) exposed to trichloroethylene (TCE) soot and different fractions

Journal article Biomarkers · January 1, 2001 It has previously been shown that trichloroethylene (TCE) soot extracts cause dioxin-like toxic effects in medaka fish (Oryzias latipes) and primary liver cell culture of rainbow trout (Oncorhynchus mykiss). This study examines embryonic and larval inducti ... Full text Cite

Toxicity of methyl-tert-butyl ether to freshwater organisms.

Journal article Environmental pollution (Barking, Essex : 1987) · January 2001 Increased input of the fuel oxygenate methyl-tert-butyl ether (MTBE) into aquatic systems has led to concerns about its effect(s) on aquatic life. As part of a study conducted by University of California scientists for the State of California, the Aquatic ... Full text Cite

Estrogen signaling in trout liver: Estrogen receptor and non-estrogen receptor mediated cellular response

Journal article Marine Environmental Research · November 14, 2000 Studies were conducted to determine intracellular hepatic signaling pathways associated with estrogen exposure in rainbow trout. These studies were initiated as a first step in elucidating potential signal transduction pathways associated with exposure to ... Full text Cite

Identification and characterization of a cDNA encoding cytochrome P450 3A from the fresh water teleost medaka (Oryzias latipes).

Journal article Archives of biochemistry and biophysics · August 2000 A new member of the CYP3A gene family has been cloned from the teleost fish medaka (Oryzias latipes) by reverse-transcriptase polymerase chain reaction (RT-PCR). Degenerate primers homologous to highly conserved regions of known CYP3A sequences were used f ... Full text Cite

Determining the sensitive developmental stages of intersex induction in medaka (Oryzias latipes) exposed to 17 beta-estradiol or testosterone.

Journal article Marine environmental research · July 2000 Certain environmentally persistent compounds can adversely affect reproduction by acting as steroid hormone agonists or antagonists. The goal of the present study was to determine the developmental stage most susceptible to exogenous hormone (estradiol and ... Full text Cite

Tissue and cellular alterations in Asian clam (Potamocorbula amurensis) from San Francisco Bay: toxicological indicators of exposure and effect?

Journal article Marine environmental research · July 2000 The US Geological Survey has reported the presence of a metal contamination gradient in clam tissues, decreased condition indices, and irregular reproductive patterns have been reported in the Asian clam, Potamocorbula amurensis, from San Francisco Bay. If ... Full text Cite

Sublethal effects of chromium-VI in the Asian clam (Potamocorbula amurensis).

Journal article Marine environmental research · July 2000 Previously, we have shown that Asian clams (Potamocorbula amurensis) with highest metallic body burdens have highest prevalence of disease and lowest reproduction. The present study was designed to assess and validate potential sublethal toxicity of hexava ... Full text Cite

Spatial profiles of hsp70 proteins in Asian clam (Potamocorbula amurensis) in northern San Francisco Bay may be linked to natural rather than anthropogenic stressors.

Journal article Marine environmental research · July 2000 Multi-year investigations in northern San Francisco Bay by United States Geological Survey have linked reduced condition indices in populations of Asian clam (Potamocorbula amurensis) with elevated cadmium tissue concentrations. Our study seeks to determin ... Full text Cite

Partial hepatectomy and bile duct ligation in rainbow trout (Oncorhynchus mykiss): histologic, immunohistochemical and enzyme histochemical characterization of hepatic regeneration and biliary hyperplasia.

Journal article Toxicologic pathology · March 2000 Hepatic regeneration following partial hepatectomy (PH) and biliary hyperplasia subsequent to bile duct ligation (BDL) were characterized in rainbow trout (Oncorhynchus mykiss) by light microscopy using routine and special (immunohistochemical and enzyme h ... Full text Cite

The role of development and duration of exposure to the embryotoxicity of diazinon

Journal article Aquatic Toxicology · January 1, 2000 Medaka, Oryzias latipes, were used as a laboratory surrogate for species of concern to define the effects of diazinon exposure on teleost embryogenesis. Medaka embryos were placed in a static, non-renewal system and exposures initiated on days 1, 3, or 5 o ... Full text Cite

Hermaphroditism in laboratory-cultured albino western mosquitofish

Journal article Journal of Aquatic Animal Health · January 1, 2000 During routine inspection of a colony of albino western mosquitofish Gambusia affinis, approximately 10 females were discovered to have distended abdominal cavities. Cause for the distention was investigated by examination of tissue sections from four sacr ... Full text Cite

Thiobencarb-induced embryotoxicity in medaka (Oryzias latipes): Stage- specific toxicity and the protective role of chorion

Journal article Aquatic Toxicology · January 1, 2000 Thiobencarb (S-(4-chlorobenzyl)-N, N-diethyl thiol carbamate) has been one of the herbicides previously associated with fish kills in agricultural drains near the Sacramento/San Joaquin rivers and their Delta. This area is an important spawning ground for ... Full text Cite

Toxic responses of medaka, d-rR strain, to polychlorinated naphthalene mixtures after embryonic exposure by in ovo nanoinjection: A partial life- cycle assessment

Journal article Environmental Toxicology and Chemistry · January 1, 2000 Polychlorinated naphthalenes (PCNs) are organic compounds with some chemical properties and uses similar to polychlorinated biphenyls. Polychlorinated naphthalenes have been detected in biota from certain aquatic environments. The toxicities of several PCN ... Full text Cite

Application of whole effluent toxicity test procedures to ambient water quality assessment

Journal article Environmental Toxicology and Chemistry · January 1, 2000 The U.S. Environmental Protection Agency (U.S. EPA) protocols for conducting freshwater toxicity tests have been used in California, USA, to evaluate ambient water quality since 1986. Testing evolved from conducting broad watershed surveys for assessing th ... Full text Cite

Diazinon and chlorpyrifos in urban waterways in northern California, USA

Journal article Environmental Toxicology and Chemistry · January 1, 2000 Samples collected from urban streams in the cities of Sacramento and Stockton, California, USA, during the precipitation season were analyzed for diazinon and chlorpyrifos. Concentrations were determined with enzyme-linked immunosorbent assays specific for ... Full text Cite

Insecticide-caused toxicity to Ceriodaphnia dubia (Cladocera) in the Sacramento-San Joaquin River Delta, California, USA

Journal article Environmental Toxicology and Chemistry · January 1, 2000 In recent years, populations of resident aquatic species in California's Sacramento-San Joaquin Delta, USA, have declined appreciably in numbers. The cause of these declines is not known, but has been attributed to a number of factors including water diver ... Full text Cite

PCBs, liver lesions, and biomarker responses in adult walleye (Stizostedium vitreum vitreum) collected from Green Bay, Wisconson

Journal article Journal of Great Lakes Research · January 1, 2000 Adult walleye were collected from several locations in the Lower Fox River and Green Bay, Wisconsin (the assessment area) and two relatively uncontaminated reference locations (Lake Winnebago and Patten Lake, Wisconsin) between July and October in 1996 and ... Full text Cite

Identification of multiple isoforms of cytochrome P450 3A in medaka Oryzias latipes: Characterization and ontogeny

Journal article Marine Environmental Research · January 1, 2000 In this study, two isoforms of cytochrome P450 3A have been cloned from medaka Oryzias latipes. Screening of a cDNA library to medaka liver resulted in the identification of two full length cDNA clones containing 1750 and 2366 base pair inserts with open r ... Full text Cite

The liver of fishes as a target organ of toxic chemicals: A new look at unique structure and function in one of the 'other' vertebrates

Journal article Marine Environmental Research · January 1, 2000 With 25,000 species of fishes, it is accurate to say that most of the livers of the world are tubular in architecture. Architectural similarity exists between the adult teleost hepatic tubule, the exocrine pancreatic acinus, and elements of the fetal and j ... Full text Cite

Variations of light and temperature regimes and resulting effects on reproductive parameters in medaka (Oryzias latipes).

Journal article Biology of reproduction · November 1999 In seasonally breeding fish species, altered fecundity, fertility, and spawning interval are associated with changes in environmental cues such as temperature and photoperiod. To determine quantitative impact of these cues on a suite of reproductive endpoi ... Full text Cite

Progression of hepatic neoplasia in medaka (Oryzias latipes) exposed to diethylnitrosamine.

Journal article Carcinogenesis · June 1999 Progression of hepatic neoplasia was assessed in medaka (Oryzias latipes) following aqueous exposure to diethylnitrosamine (DEN). Larvae (2 weeks old) were exposed to 350 or 500 p.p.m. DEN for 48 h, while adults (3-6 months old) were exposed to 50 p.p.m. D ... Full text Cite

Promotion by 17β-estradiol and β-hexachlorocyclohexane of hepatocellular tumors in medaka, Oryzias latipes

Journal article Aquatic Toxicology · April 1, 1999 A feature common to many laboratory and field studies with various fish species is a higher prevalence of hepatocellular neoplasia in females than in males. During female sexual maturation, endogenous estrogens stimulate substantial increases in synthetic ... Full text Cite

Effects of maternal exposure to estrogen and PCB on different life stages of zebrafish (Danio rerio)

Journal article Ambio · February 1, 1999 PCBs have been found to impair both reproduction and development in fish. We have investigated the effects of 3 PCB congeners, 2,3,3',4,4',5,6-HpCB (PCB-190); 2,3,4,4'-TeCB (PCB-60); and 2,2',4,6,6'-PeCB (PCB-104), and the estrogenic hormone 17β-estradiol ... Cite

Editorial.

Journal article Biochemistry · January 5, 1999 Full text Link to item Cite

Field validation of hsp70 stress proteins as biomarkers in Asian clam (Potamocorbula amurensis): Is downregulation an indicator of stress?

Journal article Biomarkers · January 1, 1999 The focus of this paper is to consider the applicability of the hsp70 stress protein response as a biomarker in field studies. Stress proteins (or heat shock proteins, hsp) of the hsp70 family are induced by sublethal concentrations of a variety of environ ... Full text Cite

Enzymatic and histopathologic biomarkers as indicators of contaminant exposure and effect in Asian clam (Potamocorbula amurensis)

Journal article Biomarkers · January 1, 1999 Enzymatic and histopathologic alterations of the digestive gland, gill, gonad, and kidney were studied in Asian clam (Potamocorbula amurensis) in April, 1997 from each of four United States Geological Survey (USGS) stations in the San Francisco Estuary. St ... Full text Cite

Histopathology of adult Pacific herring in Prince William Sound, Alaska, after the Exxon Valdez oil spill

Journal article Canadian Journal of Fisheries and Aquatic Sciences · January 1, 1999 Pacific herring (Clupea pallasi) sampled from oiled sites in Prince William Sound, Alaska, U.S.A., 3 weeks after the 1989 Exxon Valdez oil spill had multifocal hepatic necrosis and significantly increased tissue concentrations of polynuclear aromatic hydro ... Full text Cite

Organophosphate-induced acetylcholinesterase inhibition and embryonic retinal cell necrosis in vivo in the teleost (Oryzias latipes).

Journal article Neurotoxicology · December 1998 Recent monitoring of the Sacramento-San Joaquin River system (CA) indicates that levels of the organophosphate pesticide, diazinon, exceed National Academy of Science guidelines and these levels result in toxicity in USEPA acute toxicity tests with Cerioda ... Cite

Technical Services Study/Fish Histopathology Damage Assessment after the Exxon Valdez Oil Spill

Journal article Exxon Valdez Oil Spill State Federal Natural Resource Damage Assessment Final Report · October 1, 1998 Tissue samples from 4 fish species examined for microscopic lesions after the Exxon Valdez oil spill revealed that fishes in Prince William Sound were significantly affected by the oil spill. In Dolly Varden char, hepatic lipidosis and megalocytosis were t ... Cite

Viral hemorrhagic septicemia virus, Ichthyophonus hoferi, and other causes of morbidity in Pacific herring Clupea pallasi spawning in Prince William Sound, Alaska, USA.

Journal article Diseases of aquatic organisms · February 1998 Pacific herring Clupea pallasi populations in Prince William Sound, Alaska, USA, declined from an estimated 9.8 x 10(7) kg in 1992 to 1.5 x 10(7) kg in 1994. To determine the role of disease in population decline, 233 Pacific herring from Prince William So ... Full text Cite

Multiple stressors in the Sacramento River watershed.

Journal article EXS · January 1998 Aquatic biota in the Sacramento River watershed are stressed by diversion of river flows, by historical mining resulting in cadmium, copper, zinc, and mercury, and, more recently, contamination by agricultural and urban chemical runoff. In addition, the pr ... Full text Cite

Architectural pattern, tissue and cellular morphology in livers of fishes: relationship to experimentally-induced neoplastic responses.

Journal article EXS · January 1998 The teleost liver is one of the most sensitive organs to show alteration in biochemistry, physiology and structure following exposure to various types of environmental pollutants. Despite the importance of this organ to environmental toxicology and to ecot ... Full text Cite

Brevetoxin-6 (PbTx-6), a nonaromatic marine neurotoxin, is a ligand of the aryl hydrocarbon receptor.

Journal article Archives of biochemistry and biophysics · July 1997 Brevetoxins (PbTx) are a family of marine polyether toxins that exert their toxic action by activating voltage-sensitive sodium channels. Two forms of brevetoxin, PbTx-2 and -3, induce hepatic cytochrome P4501A1, measured as ethoxyresorufin O-deethylase (E ... Full text Cite

Diagnostic criteria for degenerative, inflammatory, proliferative nonneoplastic and neoplastic liver lesions in medaka (Oryzias latipes): consensus of a National Toxicology Program Pathology Working Group.

Journal article Toxicologic pathology · March 1997 Diagnostic criteria are presented for degenerative, inflammatory, nonneoplastic proliferative, and neoplastic lesions in the liver of medaka (Oryzias latipes), a small fish species frequently used in carcinogenesis studies. The criteria are the consensus o ... Full text Cite

Histopathology and cytogenetic evaluation of Pacific herring larvae exposed to petroleum hydrocarbons in the laboratory or in Prince William sound, Alaska, after the Exxon Valdez oil spill

Journal article Canadian Journal of Fisheries and Aquatic Sciences · January 1, 1997 Following the 1989 Exxon Valdez oil spill in Prince William Sound, Alaska, USA, Pacific herring (Clupea pallasi) larvae sampled from oiled sites had ascites, pericardial edema, and genotoxic damage. Laboratory study confirmed that these lesions were consis ... Full text Cite

Histopathologic biomarkers in feral freshwater fish populations exposed to different types of contaminant stress

Journal article Aquatic Toxicology · January 1, 1997 Histopathologic alterations of gill, liver, and spleen were studied in feral fish from three freshwater ecosystems that experience different types of contaminant stress. East Fork Poplar Creek (EFPC), a third-order stream in East Tennessee, receives point ... Full text Cite

Histology and teratology of pink salmon larvae near the time of emergence from gravel substrate in the laboratory

Journal article Canadian Journal of Zoology · January 1, 1997 During salmonid larval development, emergence from gravel substrate is a major event marked by final yolk absorption and transition to exogenous feeding. The gross and histological changes associated with this fundamental biological process were investigat ... Full text Cite

Ascites, premature emergence, increased gonadal cell apoptosis, and cytochrome P4501A induction in pink salmon larvae continuously exposed to oil-contaminated gravel during development

Journal article Canadian Journal of Zoology · January 1, 1997 Development of pink salmon (Oncorhynchus gorbuscha) incubating in gravel contaminated with weathered Prudhoe Bay crude oil was retarded at concentrations as low as 55.2 μg oil/g gravel. Larvae exposed to various levels of oil contamination were sampled 4 w ... Full text Cite

Dioxinlike properties of a trichloroethylene combustion-generated aerosol.

Journal article Environmental health perspectives · July 1996 Conventional chemical analyses of incineration by-products identify compounds of known toxicity but often fail to indicate the presence of other chemicals that may pose health risks. In a previous report, extracts from soot aerosols formed during incomplet ... Full text Cite

In vivo modulation of 17 beta-estradiol-induced vitellogenin synthesis and estrogen receptor in rainbow trout (Oncorhynchus mykiss) liver cells by beta-naphthoflavone.

Journal article Toxicology and applied pharmacology · April 1996 Vitellogenesis or egg yolk production represents a key estrogen initiated process in oviparous vertebrates which is crucial for oocyte maturation. Previous in vitro studies have shown that cytochrome P4501A1 (CYP1A1)-inducing compounds such as beta-naphtho ... Full text Cite

Development of procedures for identifying pesticide toxicity in ambient waters: Carbofuran, diazinon, chlorpyrifos

Journal article Environmental Toxicology and Chemistry · 1996 The responses of carbofuran, diazinon, and chlorpyrifos to standard acute toxicity identification evaluation (TIE) procedures were characterized. The test species was Ceriodaphnia dubia. The TIE procedures included solid-phase extraction, recovery in metha ... Full text Cite

Induction of ras oncogene mutations and hepatocarcinogenesis in medaka (Oryzias latipes) exposed to diethylnitrosamine

Journal article Marine Environmental Research · January 1, 1996 Medaka (Oryzias latipes), a fish model for assessing the carcinogenic impact of environmental exposure to genotoxic chemicals, were subjected to 390 ppm of diethylnitrosamine (DEN) in an aqueous bath for 48 h. Histological examinations, at 5 and 7 months p ... Full text Cite

Differential effects of brevetoxin and β-naphthoflavone on xenobiotic metabolizing enzymes in striped bass (Morone saxatilis)

Journal article Aquatic Toxicology · January 1, 1996 The goal of this study was to determine the effects of brevetoxin, a polyether marine biotoxin, on xenobiotic metabolizing enzymes and to identify potential biomarkers of exposure. Brevetoxin (PbTx) is responsible for the death of millions of fish during a ... Full text Cite

In vitro modulation of 17-β-estradiol-induced vitellogenin synthesis: Effects of cytochrome P4501A1 inducing compounds on rainbow trout (Oncorhynchus mykiss) liver cells

Journal article Aquatic Toxicology · January 1, 1996 Cytochrome P4501A1 (CYP1A1) induction, particularly in liver, is a useful marker of exposure of fish to polycyclic- and halogenated-aromatic hydrocarbons. However, the relationship between toxicity and CYP1A1 induction in fish is uncertain. Some compounds ... Full text Cite

Biomarkers of hydrocarbon exposure and sublethal effects in embiotocid fishes from a natural petroleum seep in the Santa Barbara Channel

Journal article Aquatic Toxicology · January 1, 1996 Several markers of hydrocarbon exposure and sublethal effects were measured in rainbow surfperch Hypsurus caryi and rubberlip surfperch Rachochilus toxones from a shallow natural petroleum seep and from reference areas in the Santa Barbara Channel. Hydroca ... Full text Cite

Downstream gradients in bioindicator responses: Point source contaminant effects on fish health

Journal article Canadian Journal of Fisheries and Aquatic Sciences · January 1, 1996 To investigate potential causal relationships between contaminant exposure and biological responses in fish, a suite of bioindicators ranging from the biochemical to the community-level were measured in fish populations and communities downstream from a bl ... Full text Cite

Sublethal effects of the Exxon Valdez oil spill on herring embryos and larvae: Morphological, cytogenetic, and histopathological assessments, 1989-1991

Journal article Canadian Journal of Fisheries and Aquatic Sciences · January 1, 1996 Following the Exxon Valdez oil spill in Prince William Sound, Alaska, in March 1989, Pacific herring (Clupea pallasi) larvae were evaluated for sublethal damage. From 1989 to 1991, egg masses were collected from oiled and unoiled beaches and incubated to h ... Full text Cite

Trout liver slices for metabolism and toxicity studies.

Journal article Drug metabolism and disposition: the biological fate of chemicals · January 1996 Aquatic species are increasingly used in metabolism and toxicity studies, both from the perspective of potential for chemical exposure and usefulness as nonmammalian model systems. In the present study, trout liver slices were compared with freshly isolate ... Full text Cite

Pollutant responses in marine organisms (PRIMO 8)

Journal article Marine Environmental Research · January 1, 1996 This special issue consists of papers presented at the eighth international symposium on pollutant responses in marine organisms held in April 1995 in California. The contributions are divided under 11 sections: xenobiotic biotransformation enzymes - molec ... Cite

Histopathology of swimbladder noninflation in walleye (Stizostedion vitreum) larvae: role of development and inflammation

Journal article Aquaculture · December 15, 1995 Noninflation of the swimbladder is a major obstacle in culture of many fish larvae including walleye (Stizostedion vitreum). 79 walleye larvae, 4-19 days posthatch, were serially sectioned for histologic study of swimbladder development. All walleye had a ... Full text Cite

Isolation and characterization of biliary epithelial cells from rainbow trout liver.

Journal article In vitro cellular & developmental biology. Animal · November 1995 Lectin binding and density gradient centrifugation were explored for isolating epithelial cells from trout liver. Hepatocytes exhibited preferential attachment of coverslips coated with Phaseolus vulgaris erythroagglutinin. Biliary epithelial cells attache ... Full text Cite

Histological and histochemical changes in the digestive tract of white sturgeon larvae during ontogeny

Journal article Fish Physiology and Biochemistry · October 1, 1995 Ontogenetic changes in digestive tract histology and digestive enzyme histochemistry were investigated 11 to 36 days post-hatch in white sturgeon Acipenser transmontanus larvae. From initiation of exogenous feeding (12 days post-hatch), larvae were fed a c ... Full text Cite

Long-term primary culture of epithelial cells from rainbow trout Oncorhynchus mykiss) liver.

Journal article In vitro cellular & developmental biology. Animal · May 1995 Long-term primary cultures of epithelial cells from rainbow trout (Oncorhynchus mykiss) liver have been established. Nearly homogenous (> 97%) populations of hepatocytes were placed into primary culture and remained viable and proliferative for at least 70 ... Full text Cite

Aqueous sample preparation for bioassay using supercritical fluid extraction

Journal article Environmental Toxicology and Chemistry · January 1, 1995 Supercritical fluid extraction (SFE) has become a valuable tool in the preparation of environmental samples for analysis. Most applications to date have been to extract compounds from solid matrices for later analysis by gas chromatographic or high‐perform ... Full text Cite

Oxygen consumption by larval japanese medaka with inflated or uninflated swim bladders

Journal article Transactions of the American Fisheries Society · January 1, 1995 Inflation and regulation of swim bladder volume enables fish to maintain position within the water column with minimal energy cost. Larvae whose swim bladders fail to inflate must move to stay suspended and would be expected to use additional energy to mai ... Full text Cite

The effect of agricultural discharge on striped bass (Morone saxatilis) in California's Sacramento-San Joaquin drainage

Journal article Ecotoxicology · June 1, 1994 The striped bass (Morone saxatilis) population of the Sacramento-San Joaquin Delta has declined approximately 80% since the mid-1970s. This decline has been attributed to factors such as water diversions, pollution and reduced abundance of food organisms. ... Full text Cite

Hepatic cellular distribution of cytochrome P-450 IA1 in rainbow trout (Oncorhynchus mykiss): an immunohisto- and cytochemical study.

Journal article Cancer research · August 1993 Monoclonal antibody 1-12-3 reactive against scup (Stenotomus chrysops) cytochrome P450 E (a teleost CYP IA1) has been used to immunohistochemically localize CYP IA1 within hepatocytes and presumably sinusoidal endothelial and biliary epithelial cells of sc ... Cite

Acetaminophen toxicity in cultured trout liver cells. II. Maintenance of cytochrome P450 1A1.

Journal article Experimental and molecular pathology · April 1993 Acetaminophen was demonstrated to maintain cytochrome P450 1A1 (P450 1A1) in isolated rainbow trout liver cells cultured in serum-free medium. This novel finding was characterized in detail. Cultured trout liver cells retained their ability to respond to t ... Full text Cite

Acetaminophen toxicity in cultured trout liver cells. I. Morphological alterations and effects on cytochrome P450 1A1.

Journal article Experimental and molecular pathology · April 1993 To better characterize the hepatotoxicity of acetaminophen, the effects of this drug were investigated on isolated liver cells from a species relatively resistant to acetaminophen toxicity, rainbow trout. At high concentrations of acetaminophen (2-10 mM), ... Full text Cite

Chromatophoromas and chromatophore hyperplasia in Pacific rockfish (Sebastes spp.).

Journal article Cancer research · April 1993 Pacific rockfish from Cordell Bank, off central California (United States), were collected and histologically examined from 1985 to 1990. Hyperplastic and neoplastic cutaneous lesions, involving dermal chromatophores, were observed in five species; yellowt ... Cite

Biochemical and histochemical properties of hepatic tumors of rainbow trout, Oncorhynchus mykiss.

Journal article Carcinogenesis · February 1993 Biochemical and histochemical studies were conducted in aflatoxin B1-induced liver tumors in adult rainbow trout. Specific activities of the phase I enzymes, ethoxyresorufin-O-deethylase (EROD), microsomal and cytosolic epoxide hydrolase (mEH and cEH), ald ... Full text Cite

Detection of enzyme histochemical markers of hepatic preneoplasia and neoplasia in medaka (Oryzias latipes)

Journal article Aquatic Toxicology · January 1, 1993 Freeze-drying and glycolmethacrylate embedment (FDGE), without prior fixation and chemical dehydration, has proven useful in the study of the progression of lesions associated with diethylnitrosamine-induced liver neoplasia in medaka (Oryzias latipes). Loc ... Full text Cite

A purified diet for medaka (Oryzias latipes): refining a fish model for toxicological research.

Journal article Laboratory animal science · April 1992 The overall nutritional adequacy of a purified casein-based diet (PC-diet) for the medaka (Oryzias latipes) was evaluated and compared with three diets: commercially available flaked fish food (FL-diet), live newly hatched Artemia (A-diet), and a combinati ... Cite

Ultrastructural alterations in liver of medaka (Oryzias latipes) exposed to diethylnitrosamine.

Journal article Toxicologic pathology · January 1992 Liver cytotoxic alterations of adult medaka (Oryzias latipes) following short-term bath exposure (48 hr) to 500 mg/L diethylnitrosamine (DEN) were studied (days 3-21) by electron microscopy and cytochemistry. Control medaka displayed hepatic sexual dimorph ... Full text Cite

Immunocytochemical localization of cytochrome P4501A1 in liver of rainbow trout (Oncorhynchus mykiss)

Journal article Marine Environmental Research · January 1, 1992 Multiple cytochrome P450 isozymes have been characterized in livers of trout (Oncorhynchus mykiss), scup (Stenotomus chrysops) and other fishes. Of these, trout P450 LM41, and scup P450c (or P4501A1) are probably homologous, representing the dom ... Full text Cite

Chromatophoromas and Related Hyperplastic lesions in Pacific rockfish (Sebastes spp.)

Journal article Marine Environmental Research · January 1, 1992 Pacific rockfish were sampled from Cordell Bank, off central California, from 1985 to 1990. Hyperplastic and neoplastic lesions, involving dermal chromatophores, were observed in five species; yellowtail rockfish (Sebastes flavidus), bocaccio (S. paucispin ... Full text Cite

Phenotypically altered hepatocyte populations in diethylnitrosamine-induced medaka liver carcinogenesis: Resistance, growth, and fate

Journal article Marine Environmental Research · January 1, 1992 A 5-week exposure of adult medaka (Oryzias latipes) to diethylnitrosamine (DEN. 50 ppm in aquarium water), followed by recovery, in clean water, results in hepatocellular carcinoma 12-16 weeks after initiation of exposure. Serial (weekly) morphometric eval ... Full text Cite

Induction of differentiation in human myeloblastic leukemia ML-1 cells by heptachlor, a chlorinated hydrocarbon insecticide.

Journal article Toxicology and applied pharmacology · June 1991 Effects of heptachlor, an organochlorine pesticide, on human myeloblastic leukemia ML-1 cells were determined. Similar to 12-O-tetradecanoylphorbol-13-acetate (TPA), a known tumor promoter, heptachlor induced cell adherence and formation of extended cytopl ... Full text Cite

Fish-based biomonitoring to determine toxic characteristics of complex chemical mixtures: documentation of bioremediation at a pesticide disposal site

Journal article Aquatic Toxicology · January 1, 1991 Medaka (Oryzias latipes) embryo and larval short-term bioassays were used to rapidly demonstrate changes in toxicity of aqueous soil extracts at a pesticide disposal site before and after bioremediation. By chemical analysis, concentrations of most pestici ... Full text Cite

Cytotoxicity phase of diethylnitrosamine-induced hepatic neoplasia in medaka.

Journal article Cancer research · September 1990 Adult Oryzias latipes were exposed to 50 mg of diethylnitrosamine per liter of water for 5 wk and then transferred to clean water for an additional 15 wk. Response of the liver during the first 6 wk were analyzed by enzyme histochemistry and by high-resolu ... Cite

Isolated trout liver cells: establishing short-term primary cultures exhibiting cell-to-cell interactions.

Journal article In vitro cellular & developmental biology : journal of the Tissue Culture Association · March 1990 Composition and interactions of cell types in rainbow trout (Oncorhynchus mykiss) liver digested with collagenase and cultured in serum-free media were investigated. Suspensions obtained after digesting trout liver with collagenase contained all the cell t ... Full text Cite

Age-dependent changes in toxicity of N-nitroso compounds to Japanese medaka (Oryzias latipes) embryos

Journal article Aquatic Toxicology · January 1, 1990 Three different developmental stages of embryonated eggs of Oryzias latipes were exposed to 2-h pulses of a number of different concentrations of MNU, MNNG, and DENA. Lethality and teratogenic endpoints were assessed through 24 h posthatch. MNU (≥2.5 mM) a ... Full text Cite

Metabolism of endogenous and xenobiotic aldehydes by rainbow trout (Oncorhynchus mykiss) liver fractions

Journal article Aquatic Toxicology · January 1, 1990 Aldehyde dehydrogenase (ALDH) specific activity was measured in crude homogenates, post-mitochondrial supernatants, cytosolic and microsomal fractions of trout liver, using a number of endogenous and xenobiotic aldehydes and both NAD+ and NADP Full text Cite

Effect of incubation temperature on oxygen consumption and ammonia production by Japanese medaka, Oryzias latipes, eggs and newly hatched larvae

Journal article Environmental Toxicology and Chemistry · January 1, 1990 To develop a database for use in designing sealed‐container bioassays, oxygen consumption and ammonia production rates were compared for eggs and newly hatched larvae of Japanese medaka (Oryzias latipes). At 23, 26 and 29°C, the total amount of oxygen requ ... Full text Cite

Cytochrome P450IA1 induction and localization in endothelium of vertebrate (teleost) heart.

Journal article Molecular pharmacology · November 1989 Previous studies have shown that high levels of cytochrome P450 can occur in cardiac microsomes of vertebrates [Mol. Pharmacol. 21:517-526, (1982)]. Here we identify the dominant cardiac P450 in the marine fish scup as P450E, a teleost representative of P4 ... Full text Cite

DNA repair synthesis in isolated rainbow trout liver cells.

Journal article Carcinogenesis · June 1989 Isolated trout liver cells were treated with lysolecithin to produce an in situ system for characterizing DNA repair in teleosts. In this preparation, the integrity of the plasma membrane is altered, nuclei remain intact, and the concentrations of dNTPs an ... Full text Cite

Functional units in rainbow trout (Salmo gairdneri, Richardson) liver: III. Morphometric analysis of parenchyma, stroma, and component cell types.

Journal article The American journal of anatomy · May 1989 Hepatic stroma and parenchyma with its component cell types were quantitatively described in adult male and female actively-spawning 5-year-old rainbow trout (Salmo gairdneri, Richardson). Point-count morphometry of glycol methacrylate sections estimated v ... Full text Cite

Toxicity and pharmacokinetics of the antibiotic fumagillin in yearling rainbow trout (Salmo gairdneri).

Journal article Toxicology and applied pharmacology · May 1989 Yearling trout were administered fumagillin dicyclohexylamine (FDCH), an antibiotic that has shown promise for controlling myxozoan parasites in fish. FDCH was fed at 0.25 or 1 g/kg food at 1.5% body weight per day for 60 days, and gill, liver, kidney, spl ... Full text Cite

Mechanism of allyl formate-induced hepatotoxicity in rainbow trout.

Journal article Toxicology and applied pharmacology · April 1989 Hepatotoxicity of allyl formate (AF) was studied in trout, to characterize the response of the teleost liver to a mammalian periportal hepatotoxicant. A dose-dependent decrease in liver nonprotein sulfhydryl (NPSH) concentration was observed at 3, 6, and 2 ... Full text Cite

Microsomal and cytosolic epoxide hydrolase and glutathione S-transferase activities in the gill, liver, and kidney of the rainbow trout, Salmo gairdneri. Baseline levels and optimization of assay conditions.

Journal article Biochemical pharmacology · March 1989 Microsomal and cytosolic epoxide hydrolase (mEH and cEH respectively) and glutathione S-transferase (GST) activities were measured in the liver, kidney, and gills of rainbow trout. Assays were optimized for time, pH, and temperature, using trans-stilbene o ... Full text Cite

The use of bioindicators for assessing the effects of pollutant stress on fish

Journal article Marine Environmental Research · January 1, 1989 The use of bioindicators in environmental pollution studies involves monitoring a suite of selected stress responses at several levels of biological organization in order to (1) assess the effects of sublethal stress on fish, (2) predict future trends (ear ... Full text Cite

Laser desorption-ionization FT-ICR mass spectrometry: DNA-adduct bases, nucleosides and nucleotides

Journal article Marine Environmental Research · January 1, 1989 DNA-adduct formation is of particular importance in the evaluation of exposure to carcinogens, since it may be the critical initial event that ultimately results in adverse effects, e.g. tumor formation; such manifestations may not be observed until months ... Full text Cite

In vivo microscopy of liver microvasculature in rainbow trout (Oncorhynchus mykiss)

Journal article Marine Environmental Research · January 1, 1989 Morphological change is an excellent biomarker of exposure to toxicants because it is the integration of biochemical and physiological injury. Zonal patterns of necrosis in the liver have also allowed mammalian pathologists to suggest the mechanism of toxi ... Full text Cite

Morphological changes in trout hepatocytes exposed to acetaminophen

Journal article Marine Environmental Research · January 1, 1989 Isolated trout hepatocytes prepared by collagenase digestion were maintained in primary culture in chemically defined, serum-free medium for 3-5 days to evaluate the influence of various agents on cell-cell interactions. Isolated cells were plated onto pla ... Full text Cite

Environmental contamination and cancer in fish

Journal article Marine Environmental Research · January 1, 1989 Field investigations of liver neoplasia in feral fishes indicate that tumor-bearing animals are of advanced age, have bottom-dwelling life style, and reside in polluted areas near urban centers. Laboratory investigations have shown that neoplasia in fish l ... Full text Cite

Characterization of BNF-inducible cytochrome P-450IA1 in cultures of rainbow trout liver cells

Journal article Marine Environmental Research · January 1, 1989 We are investigating isolated rainbow trout hepatocytes as a model system for characterizing the carcinogenic and toxic effects of xenobiotics. For these studies, it will be important to characterize cytochrome P-450IA1, the β-naphthoflavone (BNF)-inducibl ... Full text Cite

Histogenesis of dieldrin and DDT-induced hepatocellular carcinoma in Balb/c mice.

Journal article Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer · January 1989 Male, Balb/c mice were fed diets containing dieldrin (10 ppm) and DDT (100-175 ppm) for 75 weeks. Control and treated mice were serially killed and their livers analyzed by histological and histochemical procedures after 2, 4, 8, 16, 36, 52 and 75 weeks of ... Cite

Cytochrome P-450E induction and localization in gill pillar (endothelial) cells of scup and rainbow trout

Journal article Aquatic Toxicology · January 1, 1989 Administration of β-naphthoflavone (BNF) intraperitoneally to scup (Stenotomus chrysops) increased the specific content of total cytochrome P-450 (P-450) in gill microsomes nearly ten-fold. This increase in P-450 content was accompanied by an increase in m ... Full text Cite

Functional units in rainbow trout (Salmo gairdneri, Richardson) liver: II. The biliary system.

Journal article The Anatomical record · June 1988 The intrahepatic biliary system was studied in the rainbow trout (Salmo gairdneri), a teleost known to form liver neoplasms after exposure to various carcinogens. Normal adults (N = 25) were examined using light microscopic, enzyme histochemical, and trans ... Full text Cite

Immunohistochemical localization of cytochrome P-450E in liver, gill and heart of scup (Stenotomus chrysops) and rainbow trout (Salmo gairdneri)

Journal article Marine Environmental Research · January 1, 1988 Monoclonal antibody directed against a major β-naphthoflavone (BNF)-induced form of teleost cytochrome P-450, P-450E (equivalent to P-450c in rat) was used to immunolocalize this enzyme in liver, gill and heart of scup and trout. Liver sections from both s ... Full text Cite

Immunohistochemical detection of CCl4-induced, mitosis-related DNA synthesis in livers of trout and rat

Journal article Aquatic Toxicology · January 1, 1988 Monoclonal antibodies (MAB) directed against 5-bromo, 2′deoxyuridine (BrdU) were used to detect cells in which carbon tetrachloride-induced DNA synthesis had occurred in livers of rainbow trout (Salmo gairdneri) and of rats. Trout exposed to CCl4 Full text Cite

Cellular composition and ultrastructure of hepatic neoplasms induced by diethylnitrosamine in Oryzias latipes

Journal article Marine Environmental Research · January 1, 1988 In vivo exposure of young Oryzias latipes to the carcinogen, diethylnitrosamine (DENA), results in tumor formation after a brief latent period.1,2 During serial analysis of cytologic changes accompanying, neoplastic progression,3 we r ... Full text Cite

Allyl formate-induced hepatotoxicity in rainbow trout

Journal article Marine Environmental Research · January 1, 1988 Rainbow trout liver is tubular without apparent lobules. Hepatotoxins are often defined by the portion of the hepatic lobule affected. One of these, carbon tetrachloride, has been studied in trout and has produced no consistent pattern of toxicity similar ... Full text Cite

Mediators of pulmonary injury induced by inhalation of bacterial endotoxin.

Journal article The American review of respiratory disease · January 1988 The purpose of this study has been to further define the pathophysiologic aspects of lung injury caused by the inhalation of endotoxin (LPS) using the morphometric approach to identify mediators that influence distal lung structure and function. Hamsters w ... Full text Cite

Cytological changes during progression of neoplasia in selected fish species

Journal article Aquatic Toxicology · January 1, 1988 Cytological changes during progression of experimentally induced hepatic neoplasia in fishes were reviewed with emphasis on recent findings in Cyprinodon variegatus and Oryzias latipes. Hepatocytes are particularly sensitive to toxic changes during early p ... Full text Cite

Normal versus abnormal structure: considerations in morphologic responses of teleosts to pollutants.

Journal article Environmental health perspectives · April 1987 Consideration of newer more quantitative morphologic approaches to the study of aquatic pollutants can provide opportunity for collaborative/integrated studies with other subdisciplines in toxicology. Current commonly employed morphologic approaches result ... Full text Cite

JAPANESE MEDAKA LIVER TUMOR MODEL: REVIEW OF LITERATURE AND NEW FINDINGS.

Journal article Water Chlorination Environmental Impact and Health Effects · December 1, 1986 The Japanese medaka liver tumor model provides a rapid, in vivo screen for laboratory testing the carcinogenicity of aquatic pollutants. The high yield of tumors and associated brief induction period are strong arguments for using this model as an initial ... Cite

Development of alterations in hamster distal lung following exposure to fly ash from fluidized bed coal combustion: a morphometric study.

Journal article Toxicology and applied pharmacology · January 1986 Fly ash (30 mg) from an experimental fluidized bed coal combustor (FBC) was intratracheally instilled once into male Syrian golden hamsters. At 1, 3, 6, 9, or 30 days postadministration, lungs were fixed by intratracheal perfusion and tissues were processe ... Full text Cite

Skin mucous cell response to acid stress in male and female brown bullhead catfish, Ictalurus nebulosus (Lesueur)

Journal article Aquatic Toxicology · January 1, 1986 Exposure of brown bullhead catfish Ictalurus nebulosus (Lesueur) to water made acid in pH by addition of dilute sulfuric acid, removed a sexual dimorphism observed in mucosubstance-containing cells of control epidermis. When stained by the alcian blue-peri ... Full text Cite

Morphometric changes of the lung induced by inhaled bacterial endotoxin.

Journal article Experimental and molecular pathology · December 1985 Due to the ubiquitous nature of airborne endotoxin, an understanding of pulmonary alterations which follow inhalation of environmentally realistic concentrations of purified bacteria derived lipopolysaccharide (LPS) is important. Using LPS derived from Ent ... Full text Cite

Functional units in rainbow trout (Salmo gairdneri) liver: I. Arrangement and histochemical properties of hepatocytes.

Journal article The Anatomical record · October 1985 The architectural arrangement and selected histochemical properties of hepatocytes in the rainbow trout (Salmo gairdneri Richardson) were examined. Light and transmission electron microscopic (TEM) examination following fixation by portal venous perfusion ... Full text Cite

Mucosubstance histochemistry in control and acid-stressed epidermis of brown bullhead catfish, Ictalurus nebulosus (LeSueur).

Journal article The Anatomical record · August 1985 Glycoprotein components of epidermal mucous cells in control (pH 6.8) and acid-stressed (pH 5.8, 4.8, 4.0) brown bullhead catfish were studied by histochemical and autoradiographic methods. Following exposure to acid, increased numbers of epidermal mucous ... Full text Cite

Morphometric analysis of liver in rainbow trout: Quantitatively defining an organ of xenobiotic metabolism

Journal article Marine Environmental Research · January 1, 1985 The process of uptake, metabolism and eventual biliary excretion of xenobiotics by the teleost liver affords an opportunity for alteration within at least the following cells: sinusoidal endothelial, perisinoidal fat-storing (vitamin A-containing),1 Full text Cite

Effects of carbon tetrachloride on adrenocortical structure and function in guinea pigs.

Journal article Toxicology and applied pharmacology · August 1984 Studies were carried out to evaluate the effects of carbon tetrachloride (CCl4) on adrenocortical structure and function in guinea pigs. Treatment with CCl4 reduced adrenal microsomal cytochrome P-450 concentrations and markedly decreased adrenal benzo(a)p ... Full text Cite

Pulmonary toxicity associated with fly ash from fluidized bed coal combustion. II. Cellular morphometry of distal lung following a single intratracheal instillation.

Journal article Toxicology and applied pharmacology · August 1984 Fly ash from an experimental fluidized bed coal combustor (FBC) was intratracheally instilled (once) into male Syrian golden hamsters at concentrations of 0 (control), 3, 10, 30, and 100 mg/animal. Thirty days postadministration, lungs were fixed by intrat ... Full text Cite

Morphological survey of teleost organs important in carcinogenesis with attention to fixation.

Journal article National Cancer Institute monograph · May 1984 Key features of the gross and microscopic anatomy of teleost liver, digestive tract, kidney, and skin were reviewed from the world literature. Illustrations of the above and new findings were obtained from light as well as scanning and transmission electro ... Cite

Effect of age and exposure to a carcinogen on the structure of the medaka liver: a morphometric study.

Journal article National Cancer Institute monograph · May 1984 Morphometric evaluation of liver in the Japanese medaka (Oryzias latipes) was done to define components quantitatively. Analysis was performed in individual fish at: day of hatch (larval), 3-6, and 6-9 months, and 1 year. From analyses of hematoxylin and e ... Cite

Carcinogen induced unscheduled DNA synthesis in mouse hepatocytes.

Journal article Toxicologic pathology · January 1984 Mouse primary liver cell cultures were examined for evidence of unscheduled DNA synthesis (UDS) following treatment with the carcinogens; dimethylnitrosamine (DMNA), diethylnitrosamine (DENA), 2-acetylaminofluorene (2-AAF), N-methyl-N'-nitro-N-nitrosoguani ... Full text Cite

A method for the organ explant culture of fish kidney tubules

Journal article Journal of Tissue Culture Methods · December 1, 1982 A method for long-term organ explant culture of isolated fish kidney tubules from two teleost species is provided. Tubules maintained normal structures for 8 weeks when cultured in supplemented CMRL-1066 medium in controlled atmosphere chambers. © 1983 Tis ... Full text Cite

Morphologic and functional studies of mouse hepatocytes in primary culture.

Journal article The Anatomical record · November 1982 Mouse liver cells in primary culture were evaluated by high-resolution light microscopy (HRLM) and transmission electron microscopy (TEM). Cells after 2 hours of culture in L-15 medium supplemented with 10% fetal bovine serum were spherical in shape, and w ... Full text Cite

Heterogeneity in the distal nephron of the salamander (Ambystoma tigrinum): a correlated structure function study of isolated tubule segments.

Journal article The Anatomical record · September 1982 Studies on isolated perfused tubules of the tiger salamander (Ambystoma tigrinum) have shown that the distal nephron is heterogeneous with respect to function (Stoner, 1977). In this study, the initial portion of the distal tubule (diluting segment) exhibi ... Full text Cite

Ionic content and regulation of cellular volume in rat alveolar type II cells.

Journal article Journal of applied physiology: respiratory, environmental and exercise physiology · July 1982 Alveolar type II epithelial cells were isolated by elastase digestion of rat lungs and purified by centrifugal elutriation. This method yields an enriched fraction of 1 x 10(7) cells/rat containing 85% pure type II pneumocytes. Purified type II cells exhib ... Full text Cite

Mouse liver cell culture. II. Primary culture.

Journal article In vitro · October 1981 Mouse hepatocytes in primary culture were characterized. Hepatocytes were isolated by the two-step hepatic portal vein perfusion method described previously. An optimal cell attachment of 43% was noted after 2 h incubation in 10% fetal bovine serum. Minima ... Full text Cite

Mouse liver cell culture. I. Hepatocyte isolation.

Journal article In vitro · October 1981 A method for isolation of mouse liver cells by a two-step perfusion with calcium and magnesium-free Hanks' salt solution followed by a medium containing collagenase is described. Several variations of the commonly used procedure for rat liver cell isolatio ... Full text Cite

Biology of hepatocellular neoplasia in the mouse. III. Electron microscopy of safrole-induced hepatocellular adenomas and hepatocellular carcinomas.

Journal article Journal of the National Cancer Institute · August 1981 A systematic, ultrastructural analysis wsa performed on safrole-induced hepatocellular adenomas and hepatocellular carcinoma(s) (HPC) in BALB/c mice. Adenomas were heterogeneous in cell composition containing dark-staining basophilic cells, pale-staining a ... Full text Cite

Biology of hepatocellular neoplasia in the mouse. II. Sequential enzyme histochemical analysis of BALB/c mouse liver during safrole-induced carcinogenesis.

Journal article Journal of the National Cancer Institute · August 1981 Sequential alterations in enzyme histochemical profiles and reaction of hepatocytes to rapid iron overload were examined in male BALB/c mice during chronic, safrole exposure. At 24 weeks after initiation of safrole treatment, foci of enzyme-altered hepatoc ... Full text Cite

Biology of hepatocellular neoplasia in the mouse. I. Histogenesis of safrole-induced hepatocellular carcinoma.

Journal article Journal of the National Cancer Institute · August 1981 A sequential, histologic analysis of the livers of male BALB/c mice chronically fed the hepatocarcinogen safrole (4,000 ppm) was performed at 2, 4, 8, 16, 24, 36, 52, and 75 weeks. The transplantability of selected lesions to syngeneic hosts was also asses ... Full text Cite

Morphology of the buccopharyngeal portion of the gill in the fathead minnow Pimephales promelas (Rafinesque).

Journal article The Anatomical record · May 1981 Buccopharyngeal epithelium covering gill arches and gill rakers of the fathead minnow was studied by light microscopic, scanning, and transmission electron microscopic techniques. Mature mucous cells in goblet pattern and nonmucus containing cells were in ... Full text Cite

Effects of acid-stress on epidermal mucous cells of the brown bullhead Ictalurus nebulosus (LeSeur): a morphometric study.

Journal article The Anatomical record · May 1981 The effects of acid water on alcian blue-periodic acid Schiff's stainable mucosubstances of epidermis from the anterior abdominal wall of brown bullhead catfish, Ictalurus nebulosus, were studied. Standard morphometric techniques were used to determine muc ... Full text Cite

Renal tubular carcinoma; animal model: chemically induced renal tubular carcinoma in rats.

Journal article The American journal of pathology · July 1980 Cite

Gamma glutamyl transpeptidase in safrole-induced, presumptive premalignant mouse hepatocytes

Journal article Carcinogenesis · February 1, 1980 A histochemical procedure was used to determine the presence of γ-glutamyl transpeptidase (GGT) in the livers of control, regenerating and carcinogen-treated mice. Young Balb/c mice were fed safrole, a naturally occurring hepatocarcinogen (0.4% w/w), for o ... Full text Cite

Opposite effects of lead on chemical carcinogenesis in kidney and liver of rats.

Journal article Bulletin of environmental contamination and toxicology · November 1979 Full text Cite

Iron negative foci and nodules in safrole-exposed mouse liver made siderotic by iron-dextran injection.

Journal article Pathology, research and practice · May 1979 A procedure for the production of mouse hepatic siderosis is described which results in extensive iron deposition in all lobular zones. Mice exposed to safrole for 24 weeks displayed basophilic and acidophilic foci which did not accumulate iron. 36 weeks o ... Full text Cite

Biochemical and ultrastructural changes in teleost liver following subacute exposure to PCB.

Journal article Journal of environmental pathology and toxicology · March 1979 The response of the channel catfish liver to subacute exposure of polychlorinated biphenyls was evaluated using electron microscopic and biochemical techniques. After 21 days, treated fish displayed elevated microsomal enzyme activities. Morphologically, t ... Cite

Studies on the cellular toxicity of polychlorinated biphenyls (PCBs). I. Effect of PCBs on microsomal enzymes and on synthesis and turnover of microsomal and cytoplasmic lipids of rat liver- a morphological and biochemical study.

Journal article Virchows Archiv. B, Cell pathology · June 1978 The acute effects of the PCB (polychlorinated biphenyls) mixture (Aroclor 1254) on microsomal enzymes and on synthesis and turnover of microsomal and cytoplasmic lipids of rat liver were investigated. Six daily i.p. injections of 25 and 50 mg PCB/kg body w ... Full text Cite

Ultrastructural alterations in the eggshell gland epithelium of the mallard duck after chronic exposure to DDT

Journal article Environmental Pollution 1970 · January 1, 1978 The effects of chronic ingestion of DDT were studied on the ultrastructure of the eggshell gland of the mallard duck. Ultrastructural changes seen were oedema of the Type II epithelial cells, as demonstrated by decreased electron density, and vacuoles in t ... Full text Cite

Comparative toxicity of polychlorinated biphenyl and polybrominated biphenyl in the rat liver: light and electron microscopic alterations after subacute dietary exposure.

Journal article Journal of environmental pathology and toxicology · January 1978 The comparative toxicity of polychlorinated biphenyl (PCB) and polybrominated biphenyl (PBB) in livers was studied in male Holtzman rats. Four-week-old animals were fed at dietary levels of 0, 5, 50, or 500 ppm for 5 weeks and then sacrificed. The mean liv ... Cite

Comparison of acute response to polychlorinated biphenyl in liver of rat and channel catfish: a biochemical and morphological study.

Journal article Journal of toxicology and environmental health · January 1978 The acute response of liver of channel catfish and rat to polychlorinated biphenyl was compared on a structural and functional basis. Both the rat and the fish had elevated microsomal enzyme activities. However, in the rat the response was quantitatively g ... Full text Cite

Effects of DDT on eggshell quality and calcium adenosine triphosphatase.

Journal article Journal of toxicology and environmental health · November 1977 Adult mallard ducks were fed a diet containing 50 ppm DDT for 6 months. Eggs laid during this period were collected and eggshell weight, thickness, and calcium were determined. Chronic ingestion of DDT resulted in production of eggshells that were signific ... Full text Cite

In vitro inhibition of microsomal calcium atpase from eggshell gland of mallard duck.

Journal article Bulletin of environmental contamination and toxicology · May 1977 Full text Cite

Adenocarcinoma of the kidney. II. Enzyme histochemistry of renal adenocarcinomas induced in rats by N-(4'-fluoro-4-biphenylyl)acetamide.

Journal article Journal of the National Cancer Institute · October 1976 Activities of a broad spectrum of enzymes were studied histochemically in renal adenocarcinomas induced in young male F344 rats by chronic dietary administration of the carcinogen N(4'-fluoro-4-biphenylyl)acetamide. Enzymes included were: dehydrogenases of ... Full text Cite

Acute, subacute, and residual effects of polychlorinated biphenyl (pcb) in rats. II. Pathology and electron microscopy of liver and serum enzyme study.

Journal article Journal of toxicology and environmental health · May 1976 This study was undertaken to determine the residual effects of a polychlorinated biphenyl (Aroclor 1254) fed to male rats at dietary concentrations of 0, 5, 50, and 500 ppm in diet. The animals were treated for 4 wk (acute and subacute phase), then observe ... Full text Cite

Histopathologic alterations in shell gland accompanying DDT-induced thinning of eggshell

Journal article Environmental Pollution 1970 · January 1, 1976 Eggshell gland morphology was studied in control and DDT-treated (75 ppm) mallard ducks. Histopathologic alterations in DDT-treated ducks included oedema of villous projections, pyknosis of glandular epithelium and cytoplasmic vacuolation of lining epithel ... Full text Cite

Ultrastructure of the liver in channel catfish Ictalurus punctatus (Rafinesque)

Journal article Journal of Fish Biology · January 1, 1976 Livers of 38 channel catfish were studied by light and electron microscopy and the morphology of hepatocytes, endothelial cells, Kupffer cells, fat‐storing cells, macrophages, exocrine pancreatic cells, and epithelium of bile pre‐ductules and ducts was des ... Full text Cite

Acid phosphatase activity in subcellular fractions of fish liver exposed to methyl mercuric chloride.

Journal article Comparative biochemistry and physiology. B, Comparative biochemistry · April 1975 Full text Cite

Enzyme histochemistry of renal adenocarcinoma induced in rats by N (4' fluoro 4 biphenyl)acetamide

Journal article Journal of Histochemistry and Cytochemistry · January 1, 1975 Cite

Renal adenocarcinomas induced by N 4 4' fluorobiphenyl acetamide and their comparison with human renal adenocarcinomas

Journal article Laboratory Investigation · January 1, 1975 Chronic administration of N 4 4 fluorobiphenyl acetamide to rats results in the occurrence of renal adenocarcinomas within 1 yr following initiation of administration. These tumors arise from tubules in the cortex or outer stripe and occur following a seri ... Cite

Use of histologic and histochemical assessments in the prognosis of the effects of aquatic pollutants

Journal article Amer Soc Test Material Publ · January 1, 1973 Application of histologic and histochemical techniques to the determination of effects of aquatic pollutants upon fish tissues is discussed. Definition of terms and techniques associated with appropriate preparation of tissue specimens are given. Illustrat ... Full text Cite

Hepatic fibrosis associated with aging in four stocks of mice.

Journal article Journal of gerontology · April 1968 Full text Cite