Gregory Alan Taylor

Journal article J Immunol · November 15, 2007 Featured Publication IRG proteins, or immunity-related GTPases (also known as p47 GTPases), are a group of IFN-regulated proteins that are highly expressed in response to infection. The proteins localize to intracellular membranes including vacuoles that contain pathogens in i ... Full text Link to item Cite

The IFN-inducible GTPase LRG47 (Irgm1) negatively regulates TLR4-triggered proinflammatory cytokine production and prevents endotoxemia.

Journal article J Immunol · October 15, 2007 Featured Publication LRG47/Irgm1, a 47-kDa IFN-inducible GTPase, plays a major role in regulating host resistance as well as the hemopoietic response to intracellular pathogens. LRG47 expression in macrophages has been shown previously to be stimulated in vitro by bacterial LP ... Full text Link to item Cite

IRG proteins: key mediators of interferon-regulated host resistance to intracellular pathogens.

Journal article Cell Microbiol · May 2007 Featured Publication Immunity-related GTPases (IRG) (also known as p47 GTPases) are a family of proteins found in vertebrates, which play critical roles in mediating innate resistance to intracellular pathogens. The proteins are expressed at high levels following infection wit ... Full text Link to item Cite

Macrophage activation downregulates the degradative capacity of the phagosome.

Journal article Traffic · March 2007 Featured Publication The phagosome is key to most macrophage functions. It is the site of degradation of particulate material, of bacterial killing and the generation of peptides for antigen presentation. Despite its role at the fulcrum of the innate and acquired immune system ... Full text Link to item Cite

Control of IFN-gamma-mediated host resistance to intracellular pathogens by immunity-related GTPases (p47 GTPases).

Journal article Microbes Infect · 2007 Featured Publication IRG proteins (also known as p47 GTPases) are key mediators of interferon-gamma-induced resistance to pathogens. Absence of certain IRG proteins leads to profound susceptibility to protozoa and bacteria in mice. Underlying their roles in host resistance, IR ... Full text Link to item Cite

The p47 GTPases Igtp and Irgb10 map to the Chlamydia trachomatis susceptibility locus Ctrq-3 and mediate cellular resistance in mice.

Journal article Proc Natl Acad Sci U S A · September 19, 2006 Featured Publication Infections caused by the bacteria Chlamydia trachomatis contribute to diverse pathologies in a variety of human populations. We previously used a systemic model of C. trachomatis infection in mice to map three quantitative trait loci that influence in vivo ... Full text Link to item Cite

Human IRGM induces autophagy to eliminate intracellular mycobacteria.

Journal article Science · September 8, 2006 Featured Publication Immunity-related p47 guanosine triphosphatases (IRG) play a role in defense against intracellular pathogens. We found that the murine Irgm1 (LRG-47) guanosine triphosphatase induced autophagy and generated large autolysosomal organelles as a mechanism for ... Full text Link to item Cite

Vacuolar and plasma membrane stripping and autophagic elimination of Toxoplasma gondii in primed effector macrophages.

Journal article J Exp Med · September 4, 2006 Featured Publication Apicomplexan protozoan pathogens avoid destruction and establish a replicative niche within host cells by forming a nonfusogenic parasitophorous vacuole (PV). Here we present evidence for lysosome-mediated degradation of Toxoplasma gondii after invasion of ... Full text Link to item Cite

p47 GTPases regulate Toxoplasma gondii survival in activated macrophages.

Journal article Infect Immun · June 2005 Featured Publication The cytokine gamma interferon (IFN-gamma) is critical for resistance to Toxoplasma gondii. IFN-gamma strongly activates macrophages and nonphagocytic host cells to limit intracellular growth of T. gondii; however, the cellular factors that are required for ... Full text Link to item Cite

Autophagy is a defense mechanism inhibiting BCG and Mycobacterium tuberculosis survival in infected macrophages.

Journal article Cell · December 17, 2004 Featured Publication Mycobacterium tuberculosis is an intracellular pathogen persisting within phagosomes through interference with phagolysosome biogenesis. Here we show that stimulation of autophagic pathways in macrophages causes mycobacterial phagosomes to mature into phag ... Full text Link to item Cite

Elevated expression of a subset of interferon inducible genes in primary bone marrow cells expressing p185 Bcr-Abl versus p210 Bcr-Abl by DNA microarray analysis.

Journal article Leuk Res · March 2004 Featured Publication p185 Bcr-Abl has a more aggressive biological/clinical leukemia phenotype than p210 Bcr-Abl. In this study, we examined differential gene expression using microarrays to determine if upregulation or downregulation of specific genes may explain the distinct ... Full text Link to item Cite

STAT1 is essential for antimicrobial effector function but dispensable for gamma interferon production during Toxoplasma gondii infection.

Journal article Infect Immun · March 2004 Featured Publication The opportunistic protozoan Toxoplasma gondii is a prototypic Th1-inducing pathogen inducing strong gamma interferon (IFN-gamma) cytokine responses that are required to survive infection. Intracellular signaling intermediate STAT1 mediates many effects of ... Full text Link to item Cite

p47 GTPases: regulators of immunity to intracellular pathogens.

Journal article Nat Rev Immunol · February 2004 Featured Publication Activation of the innate immune system by interferon-gamma (IFN-gamma is crucial for host resistance to infection. IFN-gamma induces the expression of a wide range of mediators that undermine the ability of pathogens to survive in host cells, including a n ... Full text Link to item Cite

Mice deficient in LRG-47 display increased susceptibility to mycobacterial infection associated with the induction of lymphopenia.

Journal article J Immunol · January 15, 2004 Featured Publication Although IFN-gamma is essential for host control of mycobacterial infection, the mechanisms by which the cytokine restricts pathogen growth are only partially understood. LRG-47 is an IFN-inducible GTP-binding protein previously shown to be required for IF ... Full text Link to item Cite

Absence of interferon-gamma-inducible gene IGTP does not significantly alter the development of chagasic cardiomyopathy in mice infected with Trypanosoma cruzi (Brazil strain).

Journal article J Parasitol · December 2003 Featured Publication Interferon-gamma (IFN-gamma) contributes to host resistance during acute infection with Trypanosoma cruzi, the causative agent of Chagas' disease. Inducibly expressed guanosine triphosphatase (IGTP), a 48-kDa guanosine triphosphatase (GTPase), is a member ... Full text Link to item Cite

Immune control of tuberculosis by IFN-gamma-inducible LRG-47.

Journal article Science · October 24, 2003 Featured Publication Interferon-gamma (IFN-gamma) provides an essential component of immunity to tuberculosis by activating infected host macrophages to directly inhibit the replication of Mycobacterium tuberculosis (Mtb). IFN-gamma-inducible nitric oxide synthase 2 (NOS2) is ... Full text Link to item Cite

The function of gamma interferon-inducible GTP-binding protein IGTP in host resistance to Toxoplasma gondii is Stat1 dependent and requires expression in both hematopoietic and nonhematopoietic cellular compartments.

Journal article Infect Immun · December 2002 Featured Publication IGTP is a member of the 47-kDa family of gamma interferon (IFN-gamma)-induced GTPases. We have previously shown that IGTP is critical for host resistance to Toxoplasma gondii infection. In the present study, we demonstrate that T. gondii-induced IGTP expre ... Full text Link to item Cite

P311 induces a TGF-beta1-independent, nonfibrogenic myofibroblast phenotype.

Journal article J Clin Invest · November 2002 Featured Publication P311, also called PTZ17, was identified by suppressive subtraction hybridization as potentially involved in smooth muscle (SM) myogenesis. P311 is an 8-kDa protein with several PEST-like motifs found in neurons and muscle. P311 transfection into two fibrob ... Full text Link to item Cite

Gamma interferon-induced inhibition of Toxoplasma gondii in astrocytes is mediated by IGTP.

Journal article Infect Immun · September 2001 Featured Publication Toxoplasma gondii is an important pathogen in the central nervous system, causing a severe and often fatal encephalitis in patients with AIDS. Gamma interferon (IFN-gamma) is the main cytokine preventing reactivation of Toxoplasma encephalitis in the brain ... Full text Link to item Cite

Inactivation of LRG-47 and IRG-47 reveals a family of interferon gamma-inducible genes with essential, pathogen-specific roles in resistance to infection.

Journal article J Exp Med · July 16, 2001 Featured Publication The cytokine interferon (IFN)-gamma regulates immune clearance of parasitic, bacterial, and viral infections; however, the underlying mechanisms are poorly understood. Recently, a family of IFN-gamma-induced genes has been identified that encode 48-kD GTP- ... Full text Link to item Cite

Regulation of P311 expression by Met-hepatocyte growth factor/scatter factor and the ubiquitin/proteasome system.

Journal article J Biol Chem · February 11, 2000 Featured Publication P311 is a mouse cDNA originally identified for its high expression in late-stage embryonic brain and adult cerebellum, hippocampus, and olfactory bulb. The protein product of P311, however, had not been identified previously, and its function remains unkno ... Full text Link to item Cite

Pathogen-specific loss of host resistance in mice lacking the IFN-gamma-inducible gene IGTP.

Journal article Proc Natl Acad Sci U S A · January 18, 2000 Featured Publication Interferon-gamma (IFN-gamma) is critical for defense against pathogens, but the molecules that mediate its antimicrobial responses are largely unknown. IGTP is the prototype for a family of IFN-gamma-regulated genes that encode 48-kDa GTP-binding proteins ... Full text Link to item Cite

The von Hippel-Lindau tumor suppressor gene inhibits hepatocyte growth factor/scatter factor-induced invasion and branching morphogenesis in renal carcinoma cells.

Journal article Mol Cell Biol · September 1999 Featured Publication Loss of function in the von Hippel-Lindau (VHL) tumor suppressor gene occurs in familial and most sporadic renal cell carcinomas (RCCs). VHL has been linked to the regulation of cell cycle cessation (G(0)) and to control of expression of various mRNAs such ... Full text Link to item Cite

Evidence for a role of Met-HGF/SF during Ras-mediated tumorigenesis/metastasis.

Journal article Oncogene · October 22, 1998 Featured Publication Aberrations in Met-hepatocyte growth factor/scatter factor (HGF/SF) signaling have been implicated in the acquisition of tumorigenic and metastatic phenotypes. Here we show that murine NIH3T3 and C127 cells transformed by the Ras oncogene overexpress the M ... Full text Link to item Cite

Decreased fibronectin expression in Met/HGF-mediated tumorigenesis.

Journal article Oncogene · September 3, 1998 Featured Publication The tyrosine kinase receptor Met and its ligand, hepatocyte growth factor (HGF)/scatter factor are involved in the etiology and progression of a number of human cancers. Coexpression of Met and HGF in mesenchymal cells increases the tumorigenic and metasta ... Full text Link to item Cite

The inducibly expressed GTPase localizes to the endoplasmic reticulum, independently of GTP binding.

Journal article J Biol Chem · April 18, 1997 Featured Publication The inducibly expressed GTPase (IGTP) is representative of a newly identified group of interferon gamma-inducible GTPases, whose functions are currently unknown. We have begun to address the cellular function of IGTP by examining its subcellular distributi ... Full text Link to item Cite

Degradation of the Met tyrosine kinase receptor by the ubiquitin-proteasome pathway.

Journal article Mol Cell Biol · February 1997 Featured Publication The Met tyrosine kinase receptor is a widely expressed molecule which mediates pleiotropic cellular responses following activation by its ligand, hepatocyte growth factor/scatter factor (HGF/SF). In this communication we demonstrate that significant Met de ... Full text Link to item Cite

Cloning and characterization of two yeast genes encoding members of the CCCH class of zinc finger proteins: zinc finger-mediated impairment of cell growth.

Journal article Gene · October 3, 1996 Featured Publication Members of the CCCH zinc finger (Zf) protein family have in common two or more repeats of a novel Zf motif consisting of Cys and His residues in the form Cx8Cx5Cx3H [where x is a variable amino acid (aa)]. We used a degenerate polymerase chain reaction (PC ... Full text Link to item Cite

Identification of a novel GTPase, the inducibly expressed GTPase, that accumulates in response to interferon gamma.

Journal article J Biol Chem · August 23, 1996 Featured Publication Interferon gamma is a pleiotropic cytokine that regulates many immune functions. We have identified a novel protein, inducibly expressed GTPase (IGTP), whose expression was regulated by interferon gamma in macrophages. In mouse RAW 264.7 macrophages, IGTP ... Full text Link to item Cite

A pathogenetic role for TNF alpha in the syndrome of cachexia, arthritis, and autoimmunity resulting from tristetraprolin (TTP) deficiency.

Journal article Immunity · May 1996 Featured Publication Tristetraprolin (TTP) is a widely expressed potential transcription factor that contains two unusual CCCH zinc fingers and is encoded by the immediate-early response gene, Zfp-36. Mice made deficient in TTP by gene targeting appeared normal at birth, but s ... Full text Link to item Cite

Mitogens stimulate the rapid nuclear to cytosolic translocation of tristetraprolin, a potential zinc-finger transcription factor.

Journal article Mol Endocrinol · February 1996 Featured Publication Tristetraprolin (TTP) is the prototype of a group of potential transcription factors that contain two or more unusual CCCH zinc fingers. TTP is encoded by the immediate-early response gene Zfp-36, which is rapidly induced in fibroblasts in response to insu ... Full text Link to item Cite

Promoter analysis of Zfp-36, the mitogen-inducible gene encoding the zinc finger protein tristetraprolin.

Journal article J Biol Chem · October 20, 1995 Featured Publication The gene encoding the putative zinc finger protein tristetraprolin (TTP), Zfp-36, is rapidly induced by a variety of mitogens and growth factors. We show here that 77 base pairs 5' of the transcription start site are sufficient for full serum inducibility ... Full text Link to item Cite

Phosphorylation of tristetraprolin, a potential zinc finger transcription factor, by mitogen stimulation in intact cells and by mitogen-activated protein kinase in vitro.

Journal article J Biol Chem · June 2, 1995 Featured Publication Tristetraprolin (TTP) is a potential transcription factor that contains three PPPPG repeats and two putative CCCH zinc fingers. TTP is encoded by the early response gene Zfp-36, which is highly expressed in response to growth factors and in several hematop ... Full text Link to item Cite

Zinc inhibits turnover of labile mRNAs in intact cells.

Journal article J Cell Physiol · March 1995 Featured Publication For immediate early genes such as the c-fos proto-oncogene, mRNA breakdown is very rapid and is largely responsible for the transient nature of mRNA accumulation after transcription is stimulated. We found that in several types of cultured cells and in mic ... Full text Link to item Cite

The human TTP protein: sequence, alignment with related proteins, and chromosomal localization of the mouse and human genes.

Journal article Nucleic Acids Res · June 25, 1991 Featured Publication Full text Link to item Cite