Scholars@Duke
Gordon G. Hammes

Gordon G. Hammes

University Distinguished Service Professor Emeritus of Biochemistry
Biochemistry
hamme001@mc.duke.edu
Box 3711 Med Ctr, Durham, NC 27710
436A Nanaline H Duke, Durham, NC 27710

Scholarly Works - Journal articles

Conformational selection or induced fit: a flux description of reaction mechanism.

Journal article Proc Natl Acad Sci U S A · August 18, 2009 The mechanism of ligand binding coupled to conformational changes in macromolecules has recently attracted considerable interest. The 2 limiting cases are the "induced fit" mechanism (binding first) or "conformational selection" (conformational change firs ... Full text Link to item Cite

How do enzymes really work?

Journal article J Biol Chem · August 15, 2008 Full text Link to item Cite

Single-molecule and transient kinetics investigation of the interaction of dihydrofolate reductase with NADPH and dihydrofolate.

Journal article Proc Natl Acad Sci U S A · March 2, 2004 The interaction of dihydrofolate (H(2)F) and NADPH with a fluorescent derivative of H(2)F reductase (DHFR) was studied by using transient and single-molecule techniques. The fluorescent moiety Alexa 488 was attached to the structural loop that closes over ... Full text Link to item Cite

Walter Kauzmann: an undergraduate perspective.

Journal article Biophys Chem · September 2003 Full text Link to item Cite

Interaction of dihydrofolate reductase with methotrexate: ensemble and single-molecule kinetics.

Journal article Proc Natl Acad Sci U S A · October 15, 2002 Featured Work The thermodynamics and kinetics of the interaction of dihydrofolate reductase (DHFR) with methotrexate have been studied by using fluorescence, stopped-flow, and single-molecule methods. DHFR was modified to permit the covalent addition of a fluorescent mo ... Full text Link to item Cite

Multiple conformational changes in enzyme catalysis.

Journal article Biochemistry · July 2, 2002 Featured Work Understanding the molecular mechanisms of enzyme catalysis and allosteric regulation has been a primary goal of biochemistry for many years. The dynamics of these processes, approached through a variety of kinetic methods, are discussed. The results obtain ... Full text Link to item Cite

Toxicity of a formulation of the insecticide indoxacarb to the tarnished plant bug, Lygus lineolaris (Hemiptera: Miridae), and the big-eyed bug, Geocoris punctipes (Hemiptera: Lygaeidae).

Journal article Pest Manag Sci · January 2002 Indoxacarb is a new oxadiazine insecticide that has shown outstanding field insecticidal activity. The toxicity of a 145 g litre-1 indoxacarb SC formulation (Steward) was studied on the tarnished plant bug Lygus lineolaris and the big-eyed bug Geocoris pun ... Full text Link to item Cite

Editorial.

Journal article Biochemistry · January 5, 1999 Full text Link to item Cite

A golden era for understanding enzyme mechanisms.

Journal article Protein Sci · March 1998 Featured Work Full text Link to item Cite

Transient kinetic approaches to enzyme mechanisms.

Journal article Methods Enzymol · 1995 Featured Work Full text Link to item Cite

Membrane-protein structural mapping of chloroplast coupling factor in asolectin vesicles.

Journal article Biochemistry · October 23, 1990 Featured Work The spatial relationship of specific sites on chloroplast coupling factor, reconstituted in asolectin vesicles, to the bilayer surface has been studied with fluorescence methods. Fluorescence resonance energy transfer measurements have been used to map the ... Full text Link to item Cite

Rotational dynamics of chloroplast ATP synthase in phospholipid vesicles.

Journal article Biochemistry · April 3, 1990 Featured Work The rotational dynamics of the purified dicyclohexylcarbodiimide-sensitive H(+)-ATPase (DSA) reconstituted into phospholipid vesicles and of the DSA coreconstituted with the proton pump bacterio-rhodopsin were examined by using the technique of time-resolv ... Full text Link to item Cite

The ATP binding site of the yeast plasma membrane proton-translocating ATPase.

Journal article J Biol Chem · January 25, 1990 Featured Work Photoaffinity labeling of the active site of the yeast plasma membrane H(+)-ATPase has been studied with 2-azido-AMP and 2-azido-ATP. The ATPase activity of the enzyme decreases as the time of photolysis of the photoactive nucleotides in the presence of th ... Link to item Cite

Homology analysis of the protein sequences of fatty acid synthases from chicken liver, rat mammary gland, and yeast.

Journal article Proc Natl Acad Sci U S A · November 1989 Homology analyses of the protein sequences of chicken liver and rat mammary gland fatty acid synthases [acyl-CoA:malonyl-CoA C-acyltransferase (decarboxylating, oxoacyl- and enoyl-reducing and thioester hydrolyzing), EC 2.3.1.85] and yeast fatty acid synth ... Full text Link to item Cite

Cloning and expression of the yeast plasma membrane ATPase in Escherichia coli.

Journal article J Biol Chem · August 25, 1989 The yeast plasma membrane ATPase gene PMA1 was cloned into Escherichia coli using the high expression tac and T7 promoters. The gene product is toxic to the bacterial cell leading to very low expression levels and arrested growth of the host cell within mi ... Link to item Cite

Molecular cloning and sequencing of chicken liver fatty acid synthase cDNA.

Journal article Proc Natl Acad Sci U S A · June 1989 The complete amino acid sequence of chicken liver fatty acid synthase [acyl-CoA:malonyl-CoA C-acyltransferase (decarboxylating, oxoacyl- and enoyl-reducing, and thioester-hydrolyzing), EC 2.3.1.85] has been determined from the corresponding cDNA sequence. ... Full text Link to item Cite

Amino acid sequences of pyridoxal 5'-phosphate binding sites and fluorescence resonance energy transfer in chicken liver fatty acid synthase.

Journal article Biochemistry · May 2, 1989 The amino acid sequences associated with pyridoxal 5'-phosphate binding sites in chicken liver fatty acid synthase have been determined: a site whose modification causes selective inhibition of the enoyl reductase activity and a site (site I) that is not a ... Full text Link to item Cite

Structural map of the dicyclohexylcarbodiimide site of chloroplast coupling factor determined by resonance energy transfer.

Journal article Biochemistry · April 4, 1989 Fluorescence resonance energy-transfer measurements were made on the membrane-bound chloroplast coupling factor. The distances from the N,N'-dicyclohexylcarbodiimide-binding site on the membrane-bound portion of the enzyme (CF0) to the vesicle surface and ... Full text Link to item Cite

Topology of the yeast plasma membrane proton-translocating ATPase.

Journal article J Biol Chem · January 5, 1989 Four proteases have been used to assess the topology of the H+-ATPase from Saccharomyces cerevisiae reconstituted into phosphatidylserine vesicles. Limited proteolysis by trypsin and alpha-chymotrypsin inactivates the enzyme and produces stable, membrane-b ... Link to item Cite

Studies of the phosphoenzyme intermediate of the yeast plasma membrane proton-translocating ATPase.

Journal article J Biol Chem · September 25, 1988 The yeast plasma membrane proton-pumping ATPase forms a phosphorylated intermediate during the hydrolysis of ATP. The fraction of enzyme phosphorylated during steady-state ATP hydrolysis was studied as a function of substrate concentration (MgATP), Mg2+ co ... Link to item Cite

Assessment of the number of nucleotide binding sites on chloroplast coupling factor 1 by the continuous variation method.

Journal article Biochemistry · September 6, 1988 The method of continuous variation (Job plot analysis) and difference absorbance spectroscopy were used to investigate the binding of 2'(3')-(trinitrophenyl)-ADP and -ATP to chloroplast coupling factor 1 (CF1). Experiments performed at a low total concentr ... Full text Link to item Cite

Molecular cloning and sequencing of DNA complementary to chicken liver fatty acid synthase mRNA.

Journal article Proc Natl Acad Sci U S A · September 1988 The cDNA corresponding to 4.18 kilobases (kb) of the mRNA of chicken liver fatty acid synthase has been cloned and sequenced. The cDNA corresponds to the 3' end of the mRNA and consists of a 1.87-kb noncoding tail and a 2.31-kb region encoding 769 amino ac ... Full text Link to item Cite

Correlation of enzymatic activities and aggregation state in chicken liver fatty acid synthase.

Journal article Biochim Biophys Acta · August 31, 1988 The relationships between the aggregation state and the enzymatic activities of chicken liver fatty acid synthase have been explored by monitoring the changes in light scattering, fluorescence, and the overall, beta-ketoacyl synthase, beta-ketoacyl reducta ... Full text Link to item Cite

Amino acid sequences of substrate-binding sites in chicken liver fatty acid synthase.

Journal article Biochemistry · June 28, 1988 The amino acid sequences of three essential regions of chicken liver fatty acid synthase have been determined: that around 4'-phosphopantetheine ("carrier" site), the substrate "loading" site containing serine, and a "waiting" site for the growing fatty ac ... Full text Link to item Cite

Function of tightly bound nucleotides on membrane-bound chloroplast coupling factor.

Journal article Biochemistry · May 17, 1988 The kinetic behavior of tightly bound nucleotides on chloroplast coupling factor from spinach was determined under phosphorylating and nonphosphorylating conditions. Chloroplast coupling factor 1 (CF1) was labeled with tightly bound radioactive ADP and/or ... Full text Link to item Cite

Characterization of three-subunit chloroplast coupling factor.

Journal article Biochemistry · January 12, 1988 The delta- and epsilon-polypeptides were removed from chloroplast coupling factor 1 (CF1). The resulting enzyme, CF1(-delta, epsilon), is a stable active ATPase containing only alpha-, beta-, and gamma-polypeptides. The dependence of the steady-state kinet ... Full text Link to item Cite

Rotation of nucleotide sites is not required for the enzymatic activity of chloroplast coupling factor 1.

Journal article Biochemistry · September 22, 1987 New heterobifunctional photoaffinity cross-linking reagents, 6-maleimido-N-(4-benzoylphenyl)hexanamide, 12-maleimido-N-(4-benzoylphenyl)dodecanamide, and 12-[14C]maleimido-N-(4-benzoylphenyl)dodecanamide, were synthesized to investigate the mechanism of AT ... Full text Link to item Cite

Amino acid sequence of the nucleotide binding region of chloroplast coupling factor 1.

Journal article Biochemistry · June 2, 1987 The labeling of chloroplast coupling factor 1 by 3'-O-(4-benzoyl)benzoyl-ATP (BzATP) was studied. When the enzyme was incubated with approximately 10 microM BzATP and 6 mM MgCl2 at pH 7.9 for approximately 20 min and passed through two Sephadex G-50 centri ... Full text Link to item Cite

Interactions between nucleotide binding sites on chloroplast coupling factor during ATP hydrolysis.

Journal article Biochemistry · April 21, 1987 The initial hydrolysis of CaATP by chloroplast coupling factor 1 was studied with the quenched-flow method. The time course of hydrolysis can be described as a first-order conversion of the enzyme to an active form followed by steady-state formation of pro ... Full text Link to item Cite

Fluorescence studies of chicken liver fatty acid synthase. Segmental flexibility and distance measurements.

Journal article J Biol Chem · October 15, 1986 The 4'-phosphopantetheine of chicken liver fatty acid synthase was specifically labeled with the fluorescent substrate analog coenzyme A 6-[7-nitrobenz-2-oxa-1,3-diazol-4-yl]aminohexanoate at low salt concentrations. A serine at the active site of the thio ... Link to item Cite

Kinetic and nuclear magnetic resonance study of the interaction of NADP+ and NADPH with chicken liver fatty acid synthase.

Journal article Biochemistry · September 23, 1986 The ionic strength dependence of the second-order rate constant for the association of reduced nicotinamide adenine dinucleotide phosphate (NADPH) and chicken liver fatty acid synthase was determined. This rate constant is 7.2 X 10(7) M-1 s-1 at zero ionic ... Full text Link to item Cite

Interaction of spin-labeled nicotinamide adenine dinucleotide phosphate with chicken liver fatty acid synthase.

Journal article Biochemistry · August 12, 1986 The spatial relationships between the four reduced nicotinamide adenine dinucleotide phosphate (NADPH) binding sites on chicken liver fatty acid synthase were explored with electron paramagnetic resonance (EPR) and spin-labeled analogues of NADP+. The anal ... Full text Link to item Cite

Chloroplast coupling factor 1: dependence of rotational correlation time on polypeptide composition.

Journal article Biochemistry · July 15, 1986 Time-resolved fluorescence depolarization measurements were made on chloroplast coupling factor 1 (CF1) labeled with pyrenylmaleimide. Rotational correlation times were determined for native CF1, for CF1 lacking epsilon and/or delta polypeptides, and for a ... Full text Link to item Cite

Steady-state ATP synthesis by bacteriorhodopsin and chloroplast coupling factor co-reconstituted into asolectin vesicles.

Journal article Proc Natl Acad Sci U S A · June 1986 A method was developed for the co-reconstitution of bacteriorhodopsin and chloroplast coupling factor in asolectin vesicles. First, bacteriorhodopsin was reconstituted from a mixture of octyl glucoside, asolectin, and protein in the presence of ethylenedia ... Full text Link to item Cite

Steady state kinetic studies of purified yeast plasma membrane proton-translocating ATPase.

Journal article J Biol Chem · May 5, 1986 The plasma membrane H+-ATPase from bakers' yeast was purified and reconstituted with phosphatidylserine. The steady state kinetics of ATP hydrolysis catalyzed by the H+-ATPase were studied over a wide range of Mg2+ and ATP concentrations. Whereas MgATP was ... Link to item Cite

Phase-lifetime spectrophotometry of deoxycholate-purified bacteriorhodopsin reconstituted into asolectin vesicles.

Journal article Biochemistry · November 19, 1985 A rapid reconstitution procedure has been developed to insert deoxycholate-purified bacteriorhodopsin (bR) into asolectin vesicles. The procedure relies on the ability of the hydrophobic resin Bio-Beads SM-2 to remove octyl glucoside from a mixture of deox ... Full text Link to item Cite

Elementary steps in the reaction mechanism of chicken liver fatty acid synthase. Acylation of specific binding sites.

Journal article J Biol Chem · November 5, 1985 The mechanism of acyl enzyme formation from acyl-CoA derivatives was studied for chicken liver fatty acid synthase in 0.1 M potassium phosphate (pH 7.0) and 1 mM EDTA at 23 degrees C. Three mechanistically important acyl-binding sites exist: a cysteine, 4' ... Link to item Cite

Binding stoichiometry and structural mapping of the epsilon polypeptide of chloroplast coupling factor 1.

Journal article Biochemistry · October 8, 1985 Fluorescent probes were attached to the single sulfhydryl residue on the isolated epsilon polypeptide of chloroplast coupling factor 1 (CF1), and the modified polypeptide was reconstituted with the epsilon-deficient enzyme. A binding stoichiometry of one e ... Full text Link to item Cite

Structural organization of chloroplast coupling factor.

Journal article Biochemistry · April 23, 1985 Fluorescence resonance energy transfer measurements have been used to construct spatial maps for the accessible sulfhydryl of the gamma subunit (dark site) and the essential tyrosine residue of the beta subunits relative to previously mapped sites on the H ... Full text Link to item Cite

Distribution of reaction intermediates on chicken liver fatty acid synthase.

Journal article Biochemistry · April 23, 1985 The distributions of covalent intermediates in the reaction cycle catalyzed by chicken liver fatty acid synthase were studied. In isotope-trapping experiments, 30% of [1-14C] acetyl-enzyme and 6.7% of [2-14C]malonyl-enzyme are converted to long-chain fatty ... Full text Link to item Cite

Investigation of nucleotide binding sites on chloroplast coupling factor 1 with 3'O-(4-benzoyl)benzoyl adenosine 5'-triphosphate.

Journal article Proc Natl Acad Sci U S A · April 1985 The subunit locations of each of the three nucleotide binding sites of soluble chloroplast coupling factor 1 have been studied with the photoaffinity label 3'-O-(4-benzoyl)benzoyl-ATP. This derivative is an effective inhibitor of ATPase activity. Photolysi ... Full text Link to item Cite

Elementary steps in the reaction mechanism of chicken liver fatty acid synthase: beta-ketoacyl reductase and enoyl reductase.

Journal article Biochemistry · January 15, 1985 The following reactions catalyzed by chicken liver fatty acid synthase have been studied with the stopped-flow method in 0.1 M potassium phosphate (pH 7.0) and 1 mM ethylenediaminetetraacetic acid at 25 degrees C by monitoring the change in NADPH fluoresce ... Full text Link to item Cite

Structural mapping of chloroplast coupling factor.

Journal article Biochemistry · November 20, 1984 Fluorescence resonance energy transfer measurements have been used to investigate the spatial relationships between the nucleotide binding sites and the gamma-subunit of the H+-ATPase from chloroplasts and the orientation of these sites with respect to the ... Full text Link to item Cite

Elementary steps in the reaction mechanism of the alpha-ketoglutarate dehydrogenase multienzyme complex from Escherichia coli: kinetics of succinylation and desuccinylation.

Journal article Biochemistry · July 3, 1984 The kinetics of the succinylation and the desuccinylation of the alpha-ketoglutarate dehydrogenase complex from Escherichia coli have been studied at 4 degrees C in 2 mM thiamin pyrophosphate, 2 mM MgCl2, and 20 mM potassium phosphate (pH 7.0) by steady-st ... Full text Link to item Cite

Elementary steps in the reaction mechanism of chicken liver fatty acid synthase. pH dependence of NADPH binding and isotope rate effect for beta-ketoacyl reductase.

Journal article J Biol Chem · June 10, 1984 The stopped flow method has been used to determine the pH dependence of the kinetics of the binding of NADPH to chicken liver fatty acid synthase over the pH range 6.0-8.5. The kinetics is consistent with a one-step binding mechanism, and the pH dependence ... Link to item Cite

Stereochemistry of the reactions catalyzed by chicken liver fatty acid synthase.

Journal article Biochemistry · April 24, 1984 The stereochemistry of the four partial reactions catalyzed by chicken liver fatty acid synthase that lead to the synthesis of palmitic acid has been determined. The reduction of acetoacetyl-CoA to 3-hydroxybutyryl-CoA by NADPH proceeds with the transfer o ... Full text Link to item Cite

Elementary steps in the reaction mechanism of chicken liver fatty acid synthase: reduced nicotinamide adenine dinucleotide phosphate binding and formation and reduction of acetoacetyl-enzyme.

Journal article Biochemistry · December 20, 1983 The kinetics of reduced nicotinamide adenine dinucleotide phosphate (NADPH) binding to fatty acid synthase from chicken liver and of the reduction of enzyme-bound acetoacetyl by NADPH (beta-ketoacyl reductase) and the steps leading to formation of the acet ... Full text Link to item Cite

Phase-lifetime spectrophotometry of membranes from ion flux mutants of Halobacterium halobium.

Journal article J Biol Chem · July 10, 1983 Phase-lifetime spectrophotometry has been used to study the rate processes associated with intermediates in the photocycling pigments in membrane vesicles of mutant strains of Halobacterium halobium. Vesicles deficient in bacteriorhodopsin, but containing ... Link to item Cite

Steady-state kinetic study of fatty acid synthase from chicken liver.

Journal article Proc Natl Acad Sci U S A · July 1983 The steady-state kinetics of chicken liver fatty acid synthase has been studied over the pH range 5.9-8.6 in 0.1 M potassium phosphate/1 mM EDTA at 25.0 degrees C. The steady-state initial velocity, v, which was determined by measuring the rate of consumpt ... Full text Link to item Cite

Fluorescent properties of pyrene bound at specific acylation sites of chicken liver fatty acid synthase.

Journal article Biochemistry · June 7, 1983 The covalent modification of chicken liver fatty acid synthase by 4-(1-pyrenyl)butyryl-CoA (PBA-CoA), a fluorescent analogue of acetyl- and malonyl-CoA, has been studied. The binding isotherms and the kinetics of inactivation suggest 2 mol of PBA-CoA/mol o ... Full text Link to item Cite

Elementary steps in the reaction mechanism of chicken liver fatty acid synthase: acetylation-deacetylation.

Journal article Biochemistry · June 7, 1983 The kinetics of the reaction of acetyl coenzyme A (AcCoA) with fatty acid synthase has been studied with a modified quenched-flow technique in 0.1 M potassium phosphate (pH 7.0), 0.5 mM ethylenediaminetetraacetic acid, and 10% glycerol (w/v) at 23 degrees ... Full text Link to item Cite

Kinetic and structural investigation of acyl-binding sites on avian fatty acid synthase.

Journal article J Biol Chem · April 25, 1983 The acyl-binding sites of avian fatty acid synthase have been further characterized using the fluorescent acetyl-CoA analog, beta-[N-(7-nitro-2,1,3-benzoxadiazol-4-yl]alanyl coenzyme A (NBDA-CoA). Binding to nonsulfhydryl sites has been studied by treatmen ... Link to item Cite

Fluorescence polarization study of the alpha-ketoglutarate dehydrogenase complex from Escherichia coli.

Journal article Biochemistry · December 7, 1982 The lipoic acids of the alpha-ketoglutarate dehydrogenase multienzyme complex from Escherichia coli have been modified with two fluorescent probes, N-(1-pyrenyl)-maleimide and 5-[[[(iodoacetyl)amino]ethyl]amino]-naphthylene-1-sulfonic acid. Time-resolved f ... Full text Link to item Cite

Unifying concept for the coupling between ion pumping and ATP hydrolysis or synthesis.

Journal article Proc Natl Acad Sci U S A · November 1982 A mechanism is proposed for the coupling between ion transport and enzyme catalysis. The basic concept is that enzymes associated with transport exist in two possible conformations. Each conformation has the potential of catalyzing the enzymatic reaction, ... Full text Link to item Cite

Investigation of reduced nicotinamide adenine dinucleotide phosphate and acyl-binding sites on avian fatty acid synthase.

Journal article Biochemistry · June 8, 1982 The binding of reduced nicotinamide adenine dinucleotide phosphate (NADPH) to chicken liver fatty acid synthase has been studied by using both fluorescence titrations and the direct binding method of forced dialysis. Four apparently identical sites are fou ... Full text Link to item Cite

Structural mapping of nucleotide binding sites on chloroplast coupling factor.

Journal article Biochemistry · February 16, 1982 Fluorescence resonance energy transfer was used to measure the distances between three nucleotide binding sites on solubilized chloroplast coupling factor from spinach and between each nucleotide site and two tyrosine residues which are important for catal ... Full text Link to item Cite

Method for studying kinetics of light-induced transport across membranes.

Journal article Proc Natl Acad Sci U S A · December 1981 A technique based on phase spectrophotometry is described for studying the rates of elementary processes associated with the light-driven transport of ions and molecules across membranes. The light-induced pumping of protons by bacteriorhodopsin reconstitu ... Full text Link to item Cite

pH dependence of adenosine 5'-triphosphate synthesis and hydrolysis catalyzed by reconstituted chloroplast coupling factor.

Journal article Biochemistry · November 24, 1981 The purified ATP-synthesizing complex from chloroplasts has been reconstituted into phospholipid vesicles with bacteriorhodopsin by use of octyl glucoside. Phosphorylation rates up to 90 mmol of ATP (mg of protein)-1 min-1 have been achieved. The dependenc ... Full text Link to item Cite

Further characterization of nucleotide binding sites on chloroplast coupling factor one.

Journal article Biochemistry · October 27, 1981 The solubilized coupling factor from spinach chloroplasts (CF1) contains one nondissociable ADP/CF1 which exchanges slowly with medium ADP in the presence of Ca2+, Mg2+, or EDTA; medium ATP also exchanges in the presence of Ca2+ or EDTA, but it is hydrolyz ... Full text Link to item Cite

Steady state kinetics of ATP synthesis and hydrolysis catalyzed by reconstituted chloroplast coupling factor.

Journal article J Biol Chem · September 10, 1981 The steady state kinetics of ATP synthesis and hydrolysis catalyzed by the chloroplast dicyclohexylcarbodiimide-sensitive ATPase reconstituted into phospholipid vesicles were studied as a function of the transmembrane proton gradient. Bacteriorhodopsin als ... Link to item Cite

Nucleotide interactions with the dicyclohexylcarbodiimide-sensitive adenosinetriphosphatase from spinach chloroplasts.

Journal article Biochemistry · June 9, 1981 The intrinsic nucleotide content of the dicyclohexylcarbodiimide-sensitive ATPase (DSA) from spinach chloroplasts and its interactions with ADP have been studied. Both partially purified and sucrose gradient purified DSA contain at least 1 mol of ADP/mol o ... Full text Link to item Cite

Calculation on fluorescence resonance energy transfer on surfaces.

Journal article Biophys J · December 1980 A general method for estimating fluorescence resonance energy transfer between distributions of donors and acceptors on surfaces is presented. Continued fraction approximants are obtained from equivalent power series expansions of the change in quantum yie ... Full text Link to item Cite

Elementary steps in the reaction mechanism of the pyruvate dehydrogenase multienzyme complex from Escherichia coli: kinetics of acetylation and deacetylation.

Journal article Biochemistry · September 2, 1980 The kinetics of the acetylation of the pyruvate dehydrogenase complex from Escherichia coli by [3-14C]pyruvate and of the deacetylation of the complex by coenzyme A have been studied by using rapid mixing-quench techniques. The time course for acetylation ... Full text Link to item Cite

Structural mapping of rabbit muscle phosphofructokinase. Distance between the adenosine cyclic 3',5'-phosphate binding site and a reactive sulfhydryl group.

Journal article Biochemistry · January 22, 1980 The cAMP binding site of rabbit muscle phosphofructokinase has been labeled with the fluorescent molecule 5'-(p-fluorosulfonylbenzoyl)-2-aza-1,N6-ethenoadenosine. The most reactive sulfhydry- group of this modified enzyme, which is catalytically active, ha ... Full text Link to item Cite

A calorimetric study of the interaction of ATP with rabbit muscle phosphofructokinase.

Journal article J Biol Chem · December 25, 1979 The heat of interaction of ATP with phosphofructokinase from rabbit muscle was determined at 25 degrees C in 0.1 M potassium phosphate, pH 7.0 and 8.0. The limiting value of the enthalpy change at high ATP concentrations was found to be -11.5 kcal mol of e ... Link to item Cite

Subunit stoichiometry and molecular weight of the pyruvate dehydrogenase multienzyme complex from Escherichia coli.

Journal article Proc Natl Acad Sci U S A · July 1979 The molar ratio of the component enzymes of the pyruvate dehydrogenase multienzyme complex from Escherichia coli was found to be 1.8:1.7:1[pyruvate decarboxylase (E1):dihydrolipoyl transacetylase (E2):dihydrolipoyl dehydrogenase (E3)]. This ratio was deter ... Full text Link to item Cite

Mechanism of action of the pyruvate dehydrogenase multienzyme complex from Escherichia coli.

Journal article Proc Natl Acad Sci U S A · October 1978 The extent of cooperativity among the polypeptide chain components in the overall reaction catalyzed by the pyruvate dehydrogenase multienzyme complex from Escherichia coli has been studied. Selective inactivation of the pyruvate dehydrogenase component wi ... Full text Link to item Cite

Mobility and distribution of a cell surface glycoprotein and its interaction with other membrane components.

Journal article Proc Natl Acad Sci U S A · July 1977 Fluorescence photobleaching recovery and immunofluorescence methods have been used to study the lateral mobility and topographical distribution of a major cell surface glycoprotein (CSP). Both endogenous CSP and fluorescent-labeled exogenous CSP bind to th ... Full text Link to item Cite

Fluorescent derivatives of the pyruvate dehydrogenase component of the Escherichia coli pyruvate dehydrogenase complex.

Journal article Biochemistry · May 3, 1977 One sulfhydryl group per polypeptide chain of the pyruvate dehydrogenase component of the pyruvate dehydrogenase multienzyme complex from Escherichia coli was selectively labeled with N-[P-(2-benzoxazoyl)phenyl]-maleimide (NBM), 4-dimethylamino-4-magnitude ... Full text Link to item Cite

Chemical cross-linking studies of chloroplast coupling factor 1.

Journal article J Biol Chem · November 25, 1976 Cross-linking reagents have been used to link covalently adjacent subunits of solubilized spinach chloroplast coupling factor 1, which is a latent ATPase. 1,5-Difluoro-2,4-dinitrobenzene, dimethyl-3,3'-dithiobispropionimidate, and dimethylsuberimidate are ... Link to item Cite

Simple model for hormone-activated adenylate cyclase systems.

Journal article Proc Natl Acad Sci U S A · April 1976 A simple model is developed to explain the activation of rat liver plasma membrane adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] by guanosine nucleotides and glucagon and the dependence of the cATALYTIC RATE ON Mg2+, H+, and substrate ... Full text Link to item Cite

A kinetic study of protein-protein interactions.

Journal article Biochemistry · March 9, 1976 Kinetic studies have been carried out of the monomer-dimer interaction of insulin, beta-lactoglobulin, and alpha-chymotrypsin using stopped-flow and temperature-jump techniques. The pH indicators bromothymol blue, bromophenol blue, and phenol red were used ... Full text Link to item Cite

A kinetic study of the subunit dissociation and reassembly of rabbit muscle phosphofructokinase.

Journal article Biochemistry · February 24, 1976 The kinetics of dissociation and reassembly of rabbit skeletal muscle phosphofructokinase has been studied using fluorescence, stopped-flow fluorescence and enzyme activity measurements. The dissociation of the fully active tetramer in 0.8 M guanidine hydr ... Full text Link to item Cite

Fluorescence energy transfer measurements between ligand binding sites of the pyruvate dehydrogenase multienzyme complex.

Journal article Biochemistry · January 27, 1976 The interaction of the pyruvate dehydrogenase multienzyme complex from Escherichia coli with 8-anilino-1-naphthalenesulfonate (ANS), pyruvate, and acetyl-CoA has been investigated using equilibrium binding, steady-state fluorescence, and fluorescence lifet ... Full text Link to item Cite

Investigation of quercetin binding sites on chloroplast coupling factor 1.

Journal article Biochemistry · January 13, 1976 The quercetin binding sites on spinach chloroplast coupling factor 1 (CF1) have been investigated using direct and competitive binding, stopped-flow, temperature-jump, and fluorescence resonance energy transfer measurements. It was found that 8-anilino-1-n ... Full text Link to item Cite

Characterization of sulfhydryl groups on chloroplast coupling factor 1 exposed by heat activation.

Journal article Biochemistry · January 13, 1976 Two sulfhydryl groups in the gamma subunit of solubilized chloroplast coupling factor 1 (CF1) are exposed by heat activating the enzyme. These two groups have been selectively labeled with [3H]-N-ethylmaleimide, N-[p-(2-benzoxazolyl)phenyl]maleimide (NBPM) ... Full text Link to item Cite

Electron microscope study of native and crosslinked rabbit muscle phosphofructokinase.

Journal article Proc Natl Acad Sci U S A · August 1975 Electron microscopy of native and dimethyl-suberimidate crosslinked rabbit muscle phosphofructokinase (EC 2.7.1.11; ATP:D-fructose-6-phosphate 1-phosphotransferase) has been carried out using negative staining with sodium phosphotungstate. The results obta ... Full text Link to item Cite

Characterization of nucleotide binding sites on chloroplast coupling factor 1.

Journal article Biochemistry · July 1975 A study of the equilibrium binding of ADP, 1,N6-ethenoadenosine diphosphate, adenylyl imidodiphosphate, and 1,N6-ethenoadenylyl imidodiphosphate to solubilized spinach chloroplast coupling factor 1 (CF1) has been carried out. All four nucleotides were foun ... Full text Link to item Cite

Fluorescence energy transfer between ligand binding sites on chloroplast coupling factor 1.

Journal article Biochemistry · July 1975 The method of fluorescence energy transfer is used to measure the distance from the tight nucleotide binding sites to the 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole reactive sites on solubilized spinach chloroplast coupling factor 1 (CF1). The fluorescent ade ... Full text Link to item Cite

Nuclear magnetic resonance study of ligand binding to Mn-aspartate transcarbamylase.

Journal article Biochemistry · May 20, 1975 Aspartate transcarbamylase from Escherichia coli has been prepared with up to four of zinc ions replaced by manganese, and the effect of this substitution on the proton nuclear magnetic resonance properties of succinate bound to the catalytic site and of c ... Full text Link to item Cite

Subunit dissociation and unfolding of rabbit muscle phosphofructokinase by guanidine by hydrochloride.

Journal article Biochemistry · April 22, 1975 The denaturation of rabbit skeletal muscle phosphofructokinase by guanidine hydrochloride has been studied using fluorescence, light scattering, and enzyme activity measurements. The transition from fully active tetramer (0.1 M potassium phosphate (pH 8.0) ... Full text Link to item Cite

Interaction of reduced nicotinamide adenine dinucleotide with beef heart s-malate dehydrogenase.

Journal article Biochemistry · March 11, 1975 The interaction of NADH with s-malate dehydrogenase isolated from beef heart was studied in 20 mM potassium phosphate (pH 6.9)-1 mM EDTA, with forced dialysis, fluorescence, and temperature-jump techniques. Measurements of the change in fluorescence of NAD ... Full text Link to item Cite

Fluorescence energy transfer between ligand binding sites on aspartate transcarbamylase.

Journal article Biochemistry · January 28, 1975 The method of fluorescence energy transfer is used to measure the distances between several sites on aspartate transcarbamylase. Both fluorescence steady-state and lifetime techniques are used. When the tryptophans on the catalytic subunit are the fluoresc ... Full text Link to item Cite

An equilibrium binding study of the interaction of fructose 6-phosphate and fructose 1,6-bisphosphate with rabbit muscle phosphofructokinase.

Journal article Biochemistry · January 28, 1975 Equilibrium binding studies of the interaction of rabbit muscle phosphofructokinase with fructose 6-phosphate and fructose 1,6-bisphosphate have been carried out at 5 degrees in the presence of 1-10 mM potassium phosphate (pH 7.0 and 8.0), 5 mM citrate (pH ... Full text Link to item Cite

Lipid specificity of beta-hydroxybutyrate dehydrogenase activation.

Journal article J Biol Chem · January 10, 1975 Beef heart mitochondrial beta-hydroxybutyrate dehydrogenase forms a catalytically active complex with lecithin and is inactive in the absence of lecithin. The specificity of the activation process was probed by studying the interaction of the enzyme with p ... Link to item Cite

Kinetics of allosteric enzymes.

Journal article Annu Rev Biophys Bioeng · 1974 Full text Link to item Cite

Receptor biophysics and biochemistry. Enzymes.

Journal article Neurosci Res Program Bull · June 1973 Link to item Cite

Aggregation of rabbit muscle phosphofructokinase.

Journal article Biochemistry · March 27, 1973 Full text Link to item Cite

Relaxation spectra of pyruvate kinase.

Journal article Biochim Biophys Acta · September 16, 1970 Full text Link to item Cite

Kinetic properties of iodinated ribonuclease A.

Journal article Biochim Biophys Acta · March 18, 1970 Full text Link to item Cite

Relaxation spectra of enzymatic reactions.

Journal article J Phys Chem · March 1967 Full text Link to item Cite

MECHANISM OF ENZYME CATALYSIS.

Journal article Nature · October 24, 1964 Full text Link to item Cite

Concerning the role of metals in enzymic transamination.

Journal article Biochim Biophys Acta · November 19, 1962 Full text Link to item Cite