Scholars@Duke
Marcella Sarzotti-Kelsoe

Marcella Sarzotti-Kelsoe

Research Professor of Integrative Immunobiology
Integrative Immunobiology
marcella.kelsoe@duke.edu
DUMC Box 102800, Durham, NC 27710
2 Genome Ct., MSRBII Rm 1089, Durham, NC 27710

Featured Works

Thymic output, T-cell diversity, and T-cell function in long-term human SCID chimeras.

Journal article Blood · August 13, 2009 Featured Work Severe combined immunodeficiency (SCID) is a syndrome of diverse genetic cause characterized by profound deficiencies of T, B, and sometimes NK-cell function. Nonablative human leukocyte antigen-identical or rigorously T cell-depleted haploidentical parent ... Full text Link to item Cite

Evaluation and recommendations on good clinical laboratory practice guidelines for phase I-III clinical trials.

Journal article PLoS Med · May 26, 2009 Featured Work Marcella Sarzotti-Kelsoe and colleagues harmonize various approaches to Good Clinical Laboratory Practice for clinical trials into a single set of recommendations. ... Full text Open Access Link to item Cite

Review of 54 patients with complete DiGeorge anomaly enrolled in protocols for thymus transplantation: outcome of 44 consecutive transplants.

Journal article Blood · May 15, 2007 Featured Work The purpose of this study was to characterize a large group of infants with complete DiGeorge anomaly and to evaluate the ability of thymus transplantation to reconstitute immune function in these infants. DiGeorge anomaly is characterized by varying defec ... Full text Link to item Cite

T-B+NK+ severe combined immunodeficiency caused by complete deficiency of the CD3zeta subunit of the T-cell antigen receptor complex.

Journal article Blood · April 15, 2007 Featured Work CD3zeta is a subunit of the T-cell antigen receptor (TCR) complex required for its assembly and surface expression that also plays an important role in TCR-mediated signal transduction. We report here a patient with T(-)B(+)NK(+) severe combined immunodefi ... Full text Link to item Cite

Neonate-primed CD8+ memory cells rival adult-primed memory cells in antigen-driven expansion and anti-viral protection.

Journal article Int Immunol · February 2006 Featured Work Immunizations early in life, when the host is most susceptible to infection, allow protective immunological memory to develop. Decreasing the dose of Cas-Br-E murine leukemia virus when priming neonatal mice results in adult-like, Type 1 protective respons ... Full text Link to item Cite

Postnatal thymus transplantation with immunosuppression as treatment for DiGeorge syndrome.

Journal article Blood · October 15, 2004 Featured Work Complete DiGeorge syndrome is a fatal congenital disorder characterized by athymia, hypoparathyroidism, and heart defects. Less than half of patients are 22q11 hemizygous. The goal of this study was to assess if immune suppression followed by postnatal thy ... Full text Link to item Cite

Glucose-regulated protein 94/glycoprotein 96 elicits bystander activation of CD4+ T cell Th1 cytokine production in vivo.

Journal article J Immunol · April 1, 2004 Featured Work Glucose-regulated protein 94 (GRP94/gp96), the endoplasmic reticulum heat shock protein 90 paralog, elicits both innate and adaptive immune responses. Regarding the former, GRP94/gp96 stimulates APC cytokine expression and dendritic cell maturation. The ad ... Full text Link to item Cite

Thymus transplantation in complete DiGeorge syndrome: immunologic and safety evaluations in 12 patients.

Journal article Blood · August 1, 2003 Featured Work Complete DiGeorge syndrome is a fatal condition in which infants have no detectable thymus function. The optimal treatment for the immune deficiency of complete DiGeorge syndrome has not been determined. Safety and efficacy of thymus transplantation were e ... Full text Link to item Cite

T cell repertoire development in humans with SCID after nonablative allogeneic marrow transplantation.

Journal article J Immunol · March 1, 2003 Featured Work Transplantation of HLA-identical or haploidentical T cell-depleted allogeneic bone marrow (BM) into SCID infants results in thymus-dependent T cell development in the recipients. Immunoscope analysis of the TCR V beta repertoire was performed on 15 SCID pa ... Full text Link to item Cite

GRP94 (gp96) and GRP94 N-terminal geldanamycin binding domain elicit tissue nonrestricted tumor suppression.

Journal article J Exp Med · December 2, 2002 Featured Work In chemical carcinogenesis models, GRP94 (gp96) elicits tumor-specific protective immunity. The tumor specificity of this response is thought to reflect immune responses to GRP94-bound peptide antigens, the cohort of which uniquely identifies the GRP94 tis ... Full text Link to item Cite

Enhanced type 1 immunity after secondary viral challenge in mice primed as neonates.

Journal article J Immunol · September 15, 2002 Featured Work The goal of infant immunization against viral infection is to develop protective long term memory responses. Priming neonatal mice with a low dose of Cas-Br-E murine leukemia virus (Cas) results in adult-like, type 1 protective responses. However, other st ... Full text Link to item Cite

CD4+Thy1- thymocytes with a Th-type 2 cytokine response.

Journal article Int Immunol · January 2001 Featured Work We have identified a small subset of CD3(+), CD4(+), CD8(-) thymocytes that do not express Thy1 (CD90). This Thy1(-) subset represents 1-3.7% of the total number of thymocytes in a naive mouse. CD4(+)Thy1(-) thymocytes express high levels of CD3, intermedi ... Full text Link to item Cite

Cellular immune responses in neonates.

Journal article Int Rev Immunol · 2000 Featured Work Reduced numbers of lymphocytes and antigen presenting cells have been described as some of the main factors responsible for antigenic tolerance or low responsiveness in neonates. However, by changing the parameters of immunization, such as dose of antigen ... Full text Link to item Cite

Induction of cytotoxic T cell responses in newborn mice by DNA immunization.

Journal article Vaccine · June 1997 Featured Work Cytotoxic T cells (CTL) play a critical role in controlling viral infections. Infection of neonatal NFSIN mice with a high dose of Cas-Br-M murine leukemia virus, a neuropathogenic type C retrovirus, results in virus-induced neurologic disease and in their ... Full text Link to item Cite

Immunologic tolerance.

Journal article Curr Opin Hematol · January 1997 Featured Work Understanding the phenomenon of immunologic tolerance and the mechanisms that control it at different stages of lymphocyte development is rapidly progressing. This review summarizes some recent findings in T-cell tolerance, focusing on neonatal and periphe ... Full text Link to item Cite

Induction of protective CTL responses in newborn mice by a murine retrovirus.

Journal article Science · March 22, 1996 Featured Work The susceptibility of neonates to virus-induced disease is thought to reflect, in part, the immaturity of their immune systems. However, inoculation of newborn mice with low doses of Cas-Br-M murine leukemia virus induced a protective cytotoxic T lymphocyt ... Full text Link to item Cite