Conference2024 Conference on Lasers and Electro-Optics, CLEO 2024 · January 1, 2024
Melanoma, the most aggressive skin cancer, is responsible for around 10,000 deaths annually.Wearedevelopingadiagnosticbiomarkerbasedonfemtosecondpump-probemicroscopy of melanin, a natural pigment found in most melanoma. ...
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Conference2024 Conference on Lasers and Electro Optics CLEO 2024 · January 1, 2024
Melanoma, the most aggressive skin cancer, is responsible for around 10,000 deaths annually.Wearedevelopingadiagnosticbiomarkerbasedonfemtosecondpump-probemicroscopy of melanin, a natural pigment found in most melanoma. ...
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ConferenceOptics Infobase Conference Papers · April 18, 2016
Nonlinear pump-probe microscopy differentiates cutaneous, vulvar and conjunctival melanomas from less dangerous lesions, and can be used to assess metastatic potential. Applications in vivo (knockout mouse models) and in human biopsy are presented. ...
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ConferenceProgress in Biomedical Optics and Imaging Proceedings of SPIE · January 1, 2016
The use of multiphoton interactions in biological tissue for imaging contrast requires highly sensitive optical measurements. These often involve signal processing and filtering steps between the photodetector and the data acquisition device, such as photo ...
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ConferenceProceedings of SPIE the International Society for Optical Engineering · January 1, 2015
We demonstrate that ultrafast pump-probe microscopy provides unique dynamics for natural iron oxide and iron hydroxide earth pigments, despite their chemical similarity. First, we conducted a pump-probe spectroscopy study on heat-treated hematite (the pure ...
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ConferenceProceedings of SPIE the International Society for Optical Engineering · January 1, 2015
The transient absorption response of melanin is a promising optically-accessible biomarker for distinguishing malignant melanoma from benign pigmented lesions, as demonstrated by earlier experiments on thin sections from biopsied tissue. The technique has ...
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ConferenceOptics Infobase Conference Papers · January 1, 2015
Enhanced control over femtosecond lasers (pulse shaping or pulse train modulation) improves contrast in tissue imaging. Pump-probe applications to melanoma diagnosis and cross phase modulation measurement in transparent tissues will be presented. ...
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ConferenceOptics Infobase Conference Papers · January 1, 2015
Pump-probe microscopy can extract molecular and structural contrast with high spatial resolution even in thick, scattering samples. We will discuss the technique’s principle and applications for imaging historic paintings and other cultural heritage object ...
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ConferenceProgress in Biomedical Optics and Imaging Proceedings of SPIE · June 12, 2013
New labeling, imaging, or analysis tools could provide new retrospective insights when applied to archived, paraffinembedded samples. Deep-tissue multiphoton microscopy of paraffin-embedded specimens is achieved using optical clearing with mineral oil. We ...
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ConferenceProgress in Biomedical Optics and Imaging Proceedings of SPIE · May 28, 2013
Phasor analysis has become a powerful tool for examining signals in fluorescence life-time microscopy (FLIM), where the analysis provides a fast, robust and intuitive means of separating different fluorescent species and mixtures thereof. In this work we a ...
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ConferenceLaser Science Ls 2012 · December 1, 2012
Nonlinear optical pump-probe microscopy is able to sensitively extract excited state dynamics from historical art pigments. This technique allows three-dimensional imaging and characterization of different pigments, with far reaching applications for conse ...
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ConferenceProgress in Biomedical Optics and Imaging Proceedings of SPIE · July 25, 2011
Nonlinear imaging takes advantage of the localized nature of the interaction to achieve high spatial resolution, optical sectioning, and deeper penetration in tissue. However, nonlinear contrast (other than fluorescence or harmonic generation) is generally ...
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ConferenceOptics InfoBase Conference Papers · December 1, 2010
We demonstrate a homodyne coherent anti-Stokes Raman scattering technique based on femtosecond laser pulse shaping (phase-cycling). This technique utilizes a self-generated non-resonant background as a local oscillator to retrieve phase information of the ...
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ConferenceOptics Infobase Conference Papers · January 1, 2010
We demonstrate a homodyne coherent anti-Stokes Raman scattering technique based on femtosecond laser pulse shaping (phase-cycling). This technique utilizes a self-generated non-resonant background as a local oscillator to retrieve phase information of the ...
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ConferenceProgress in Biomedical Optics and Imaging Proceedings of SPIE · June 1, 2009
Nonlinear microscopies (most commonly, two-photon fluorescence, second harmonic generation, and coherent anti-Stokes Raman scattering (CARS)) have had notable successes in imaging a variety of endogenous and exogenous targets in recent years. These methods ...
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ConferenceOptics Infobase Conference Papers · January 1, 2008
We have designed an interferometric acousto-optic pulse shaper capable of shaping individual pulses differently from a mode-locked laser. The design enables the measurement of weak nonlinear optical signals at megahertz (MHz) rates for imaging applications ...
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ConferenceOptics Infobase Conference Papers · January 1, 2008
Recently developed ultrafast laser pulse shaping technology allows highsensitivity measurements of nonlinear optical effects in highly scattering media. We present applications of these techniques to extract intrinsic structural, metabolic and functional c ...
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ConferenceOptics Infobase Conference Papers · January 1, 2008
Using novel femtosecond laser pulse shaping techniques and a virtually background-free detection strategy we demonstrate strong self-phase modulation signatures of neuronal activity in hippocampal brain slices without the use of exogenous contrast agents. ...
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ConferenceProgress in Biomedical Optics and Imaging Proceedings of SPIE · September 3, 2007
Even though multi-photon fluorescence microscopy offers higher resolution and better penetration depth than traditional fluorescence microscopy, its use is restricted to the detection of molecules that fluoresce. Two-photon absorption (TPA) imaging can pro ...
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ConferenceProceedings of SPIE the International Society for Optical Engineering · May 8, 2006
Multiphoton excitation fluorescence microscopy has proven to be a powerful method for non-invasive, in vivo, thick tissue imaging with molecular specificity. However, many important endogenous biomolecules do not fluoresce (NAD) or fluoresce with low effic ...
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ConferenceSpringer Series in Chemical Physics · December 1, 2004
Femtosecond laser pulse shaping permits background-free detection of two-photon absorption, which tends to refill spectral holes. This opens up new spectroscopic windows for monitoring tissue characteristics. ...
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ConferenceOptics Infobase Conference Papers · January 1, 2004
We present a new method for deep tissue imaging using nonlinear optics. Specific tailored laser pulses permit measurement of two-photon absorption or self-phase modulation with modest near-IR powers (1-10 mW), giving good penetration and molecular specific ...
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ConferenceOptics Infobase Conference Papers · January 1, 2004
Femtosecond laser pulse shaping permits background-free detection of two-photon absorption, which tends to refill spectral holes. This opens up new spectroscopic windows for monitoring tissue characteristics. ...
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Conference2004 2nd IEEE International Symposium on Biomedical Imaging Macro to Nano · January 1, 2004
Multiphoton imaging with fluorescence detection has proven to be a powerful method for moderately deep tissue imaging (less than 1 mm) with molecular specificity. However, many important endogenous biomolecules do not fluoresce (NAD) or with low efficiency ...
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ConferenceOptics Infobase Conference Papers · January 1, 2002
We have fabricated SSC-DFB laser transmitter modules, which give enough output power, and can tolerate strong reflection. They satisfy the SDH/SONET requirements for transmission up to 15 km at 2.5 Gbit/s. Because of the expected low packaging cost, the tr ...
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ConferenceEuropean Conference on Optical Communication ECOC · December 1, 2001
Single-channel 160Gb/s unrepeated transmission over 200km of non-zero dispersion shifted fiber (NZDSF) was reported using distributed Raman amplification. Clock recovery and demultiplexing were realized simultaneously by cascading a tandem electro-absorpti ...
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ConferenceTechnical Digest Summaries of Papers Presented at the Quantum Electronics and Laser Science Conference QELS 2001 · January 1, 2001
We study the motion of ultra-cold atoms in an accelerating optical lattice. We find that the tunneling dynamics are strongly affected by repeated measurement during the non-exponential time, leading to either inhibition (zeno) or enhancement (anti-zeno) of ...
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ConferenceTechnical Digest European Quantum Electronics Conference · January 1, 1998
A simple model of the band structure with a single (trapped) band separated by a bandgap from a free particle state is used to study short-time deviation from exponential decay in a quantum tunneling experiment. The system consists of ultracold sodium atom ...
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ConferenceOptics Infobase Conference Papers · January 1, 1997
We report the observation of short-time deviation from exponential decay in tunneling of ultra-cold atoms from an accelerating periodic optical potential. We measure a'flat survival probability followed by a transition to exponential decay. ...
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