Scholars@Duke
Michael Dee Gunn

Michael Dee Gunn

Professor of Medicine
Medicine, Cardiology
Duke Box 102143, Durham, NC 27710
CARL Building Room 180B, Durham, NC 27703

Featured Works

Nonclassical Monocytes Sense Hypoxia, Regulate Pulmonary Vascular Remodeling, and Promote Pulmonary Hypertension.

Journal article J Immunol · March 15, 2020 Featured Publication An increasing body of evidence suggests that bone marrow-derived myeloid cells play a critical role in the pathophysiology of pulmonary hypertension (PH). However, the true requirement for myeloid cells in PH development has not been demonstrated, and a sp ... Full text Link to item Cite

Antigen-loaded monocyte administration induces potent therapeutic antitumor T cell responses.

Journal article The Journal of clinical investigation · February 2020 Featured Publication Efficacy of dendritic cell (DC) cancer vaccines is classically thought to depend on their antigen-presenting cell (APC) activity. Studies show, however, that DC vaccine priming of cytotoxic T lymphocytes (CTLs) requires the activity of endogenous DCs, sugg ... Full text Cite

Brain immunology and immunotherapy in brain tumours.

Journal article Nat Rev Cancer · January 2020 Featured Publication Gliomas, the most common malignant primary brain tumours, remain universally lethal. Yet, seminal discoveries in the past 5 years have clarified the anatomy, genetics and function of the immune system within the central nervous system (CNS) and altered the ... Full text Link to item Cite

A Protocol for the Comprehensive Flow Cytometric Analysis of Immune Cells in Normal and Inflamed Murine Non-Lymphoid Tissues.

Journal article PLoS One · 2016 Featured Publication Flow cytometry is used extensively to examine immune cells in non-lymphoid tissues. However, a method of flow cytometric analysis that is both comprehensive and widely applicable has not been described. We developed a protocol for the flow cytometric analy ... Full text Open Access Link to item Cite

Arginine deprivation and immune suppression in a mouse model of Alzheimer's disease.

Journal article J Neurosci · April 15, 2015 Featured Publication The pathogenesis of Alzheimer's disease (AD) is a critical unsolved question; and although recent studies have demonstrated a strong association between altered brain immune responses and disease progression, the mechanistic cause of neuronal dysfunction a ... Full text Link to item Cite

Tetanus toxoid and CCL3 improve dendritic cell vaccines in mice and glioblastoma patients.

Journal article Nature · March 19, 2015 Featured Publication After stimulation, dendritic cells (DCs) mature and migrate to draining lymph nodes to induce immune responses. As such, autologous DCs generated ex vivo have been pulsed with tumour antigens and injected back into patients as immunotherapy. While DC vacci ... Full text Open Access Link to item Cite

Loss of basal cells precedes bronchiolitis obliterans-like pathological changes in a murine model of chlorine gas inhalation.

Journal article Am J Respir Cell Mol Biol · November 2013 Featured Publication Bronchiolitis obliterans (BO) is a major cause of chronic airway dysfunction after toxic chemical inhalation. The pathophysiology of BO is not well understood, but epithelial cell injury has been closely associated with the development of fibrotic lesions ... Full text Link to item Cite

A macrophage subpopulation recruited by CC chemokine ligand-2 clears apoptotic cells in noninfectious lung injury.

Journal article Am J Physiol Lung Cell Mol Physiol · May 1, 2012 Featured Publication CC chemokine ligand-2 (CCL2)/monocyte chemoattractant protein (MCP)-1 expression is upregulated during pulmonary inflammation, and the CCL2-CCR2 axis plays a critical role in leukocyte recruitment and promotion of host defense against infection. The role o ... Full text Link to item Cite

NUR who? An orphan transcription factor holds promise for monomaniacs.

Journal article Nat Immunol · July 19, 2011 Featured Publication Full text Link to item Cite

CCR2-antagonist prophylaxis reduces pulmonary immune pathology and markedly improves survival during influenza infection.

Journal article J Immunol · January 1, 2011 Featured Publication Infection with influenza virus induces severe pulmonary immune pathology that leads to substantial human mortality. Although antiviral therapy is effective in preventing infection, no current therapy can prevent or treat influenza-induced lung injury. Prev ... Full text Link to item Cite

Blood-derived inflammatory dendritic cells in lymph nodes stimulate acute T helper type 1 immune responses.

Journal article Nat Immunol · April 2009 Featured Publication T helper type 1 (T(H)1)-polarized immune responses, which confer protection against intracellular pathogens, are thought to be initiated by dendritic cells (DCs) that enter lymph nodes from peripheral tissues. Here we found after viral infection or immuniz ... Full text Link to item Cite

Identification of recombinant antibodies against multiple distinct toll-like receptors by homolog mining a single immune scFv phage library.

Journal article J Immunol Methods · January 30, 2009 Featured Publication The generation of recombinant single-chain antibodies from either non-immune or immune phage display antibody libraries is an effective means to obtain high affinity antibodies against a specific target. Non-immune libraries contain a wide variety of antib ... Full text Link to item Cite

An entirely cell-based system to generate single-chain antibodies against cell surface receptors.

Journal article J Mol Biol · May 30, 2008 Featured Publication The generation of recombinant antibodies (Abs) using phage display is a proven method to obtain a large variety of Abs that bind with high affinity to a given antigen. Traditionally, the generation of single-chain Abs depends on the use of recombinant prot ... Full text Link to item Cite

CCR2+ monocyte-derived dendritic cells and exudate macrophages produce influenza-induced pulmonary immune pathology and mortality.

Journal article J Immunol · February 15, 2008 Featured Publication Infection with pathogenic influenza virus induces severe pulmonary immune pathology, but the specific cell types that cause this have not been determined. We characterized inflammatory cell types in mice that overexpress MCP-1 (CCL2) in the lungs, then exa ... Full text Link to item Cite

CD11c(+)B220(+)Gr-1(+) cells in mouse lymph nodes and spleen display characteristics of plasmacytoid dendritic cells.

Journal article J Exp Med · October 15, 2001 Featured Publication Human plasmacytoid dendritic cells (pDCs) are major producers of IFNalpha, are activated by CpG motifs, and are believed to enter lymph nodes (LNs) via L-selectin dependent extravasation across high endothelial venules. To identify a similar murine DC type ... Full text Link to item Cite

Mice lacking expression of the chemokines CCL21-ser and CCL19 (plt mice) demonstrate delayed but enhanced T cell immune responses.

Journal article J Exp Med · January 15, 2001 Featured Publication The paucity of lymph node T cells (plt) mutation leads to a loss of CCL21 and CCL19 expression in secondary lymphoid organs. plt mice have defects in the migration of naive T cells and activated dendritic cells into the T cell zones of lymphoid organs, sug ... Full text Link to item Cite

Mice lacking expression of secondary lymphoid organ chemokine have defects in lymphocyte homing and dendritic cell localization.

Journal article J Exp Med · February 1, 1999 Featured Publication Secondary lymphoid organ chemokine (SLC) is expressed in high endothelial venules and in T cell zones of spleen and lymph nodes (LNs) and strongly attracts naive T cells. In mice homozygous for the paucity of lymph node T cell (plt) mutation, naive T cells ... Full text Link to item Cite

A B-cell-homing chemokine made in lymphoid follicles activates Burkitt's lymphoma receptor-1.

Journal article Nature · February 19, 1998 Featured Publication Secondary lymphoid organs (spleen, lymph nodes and Peyer's patches) are divided into compartments, such as B-cell zones (follicles) and T-cell zones, which provide specialized environments for specific steps of the immune response. Migration of lymphocyte ... Full text Link to item Cite

A chemokine expressed in lymphoid high endothelial venules promotes the adhesion and chemotaxis of naive T lymphocytes.

Journal article Proc Natl Acad Sci U S A · January 6, 1998 Featured Publication Preferential homing of naive lymphocytes to secondary lymphoid organs is thought to involve the action of chemokines, yet no chemokine has been shown to have either the expression pattern or the activities required to mediate this process. Here we show tha ... Full text Link to item Cite