Nikolai Petrovich Skiba | Scholars@Duke

Nikolai Petrovich Skiba
Associate Professor of Ophthalmology

My research focuses on applying mass spectrometry based proteomics to study proteins in eye tissues, cells and sub-cellular compartments to understand mechanisms of vision. An important aspect of my research is to identify proteins in different compartments of retinal photoreceptor cells, their amount and modification status at different cell states defined by the light conditions, genotype, disease etc. This information can be valuable in understanding molecular mechanisms of vision and biology of the photoreceptor cell. Another important aspect of my research is to assist basic scientist and clinicians in our department in their proteomic needs which include identification of proteins and other biomolecules in a given biological sample, detection of protein post-translational modifications and sequence variations, elucidation of protein-protein interactions and also characterization of changes in the protein concentration and composition in a biological sample at different conditions.

Current Appointments & Affiliations

Associate Professor of Ophthalmology, Ophthalmology, Clinical Science Departments 2021

Contact Information

Box 3802 Med Ctr, Durham, NC 27710
Wadsworth Bldg, Durham, NC 27710
(919) 684-8579

Background
Education, Training, & Certifications
- Postdoctoral Research Assoc, Physiology And Biophysics, University of Illinois 1991 - 1996
- Senior Scientist, Bioorganic Chemistry, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry (Russia) 1989 - 1991
- Postdoctoral Scientist, Bioorganic Chemistry, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry (Russia) 1986 - 1989
- Ph.D., Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry (Russia) 1986
- B.S., Lomonosov Moscow State Universty (Russia) 1978
Previous Appointments & Affiliations
- Associate Professor in Ophthalmology, Ophthalmology, Clinical Science Departments 2014 - 2020
- Assistant Professor in Ophthalmology, Ophthalmology, Clinical Science Departments 2007 - 2014
- Assistant Professor in Ophthalmology, Ophthalmology, Clinical Science Departments 2006 - 2007
- Assistant Research Professor in Ophthalmology, Ophthalmology, Clinical Science Departments 2005 - 2006
Academic Positions Outside Duke
- Instructor, Harvard University. 2004 - 2005
- Instructor, Harvard University. 1999 - 2001
- Research Assistant Professor, Northwestern University. 1996 - 1999
Research
Selected Grants
- RPE Exosomes in Age-related Macular Degeneration awarded by National Institutes of Health 2022 - 2025
- Identification of novel contributors to retinitis pigmentosa using metabolic and proteomic approaches awarded by University of North Carolina - Chapel Hill 2021 - 2023
- Deciphering the local effect of glaucoma risk factors on axonal mitoproteome awarded by BrightFocus Foundation 2021 - 2023
- Mechanisms of photoreceptor protein transport and compartmentalization awarded by SUNY Upstate Medical University 2021 - 2022
- The Role of RPE-derived Exosomes in Deposit Formation and ECM Modulation awarded by National Institutes of Health 2021 - 2022
- Lysosomal Enzymes in Outflow Pathway Physiology and Pathophysiology awarded by National Institutes of Health 2017 - 2022
- Identification of unique rod outer segment plasma membrane proteins awarded by National Institutes of Health 2017 - 2019
- The Role of Exosomes in Dry AMD awarded by BrightFocus Foundation 2015 - 2018
- Role of Exosomes in Glaucomatous Lamina Cribrosa Remodeling awarded by Glaucoma Research Foundation 2016 - 2017
- Ankyrin G in protein sorting between rod plasma membrane and photoreceptor discs awarded by National Institutes of Health 2010 - 2012
- Oxidative Stress and Lysosomal Function in the Outflow Pathway awarded by National Institutes of Health 2008 - 2011
- Proteome Map of the Photoreceptor Cell awarded by National Institutes of Health 2006 - 2009
Publications & Artistic Works
Selected Publications
- Academic Articles
  - Lewis, Tylor R., Sebastien Phan, Carson M. Castillo, Keun-Young Kim, Kelsey Coppenrath, William Thomas, Ying Hao, et al. “Photoreceptor disc incisures form as an adaptive mechanism ensuring the completion of disc enclosure.” Biorxiv, April 7, 2023. https://doi.org/10.1101/2023.04.06.535932.
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  - Spencer, William J., Nicholas F. Schneider, Tylor R. Lewis, Carson M. Castillo, Nikolai P. Skiba, and Vadim Y. Arshavsky. “The WAVE complex drives the morphogenesis of the photoreceptor outer segment cilium.” Proc Natl Acad Sci U S A 120, no. 12 (March 21, 2023): e2215011120. https://doi.org/10.1073/pnas.2215011120.
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  - Skiba, Nikolai P., Tylor R. Lewis, William J. Spencer, Carson M. Castillo, Andrej Shevchenko, and Vadim Y. Arshavsky. “Absolute quantification of photoreceptor outer segment proteins.” Biorxiv, January 20, 2023. https://doi.org/10.1101/2023.01.19.524794.
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  - Travis, Amanda M., Samiya Manocha, Jason R. Willer, Timothy S. Wessler, Nikolai P. Skiba, and Jillian N. Pearring. “Disrupting the ciliary gradient of active Arl3 affects rod photoreceptor nuclear migration.” Elife 12 (January 4, 2023). https://doi.org/10.7554/eLife.80533.
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  - Choi, Jin A., Hyun-Hee Ju, Jiyoung Lee, Ju-Eun Kim, Soon-Young Paik, Nikolai P. Skiba, and Ponugoti Vasantha Rao. “Increased Complement-Associated Inflammation in Cytomegalovirus-Positive Hypertensive Anterior Uveitis Patients Based on the Aqueous Humor Proteomics Analysis.” J Clin Med 11, no. 9 (April 22, 2022). https://doi.org/10.3390/jcm11092337.
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  - Choi, Jin A., Rupalatha Maddala, Shruthi Karnam, Nikolai P. Skiba, Robin Vann, Pratap Challa, and Ponugoti Vasantha Rao. “Role of vasorin, an anti-apoptotic, anti-TGF-β and hypoxia-induced glycoprotein in the trabecular meshwork cells and glaucoma.” J Cell Mol Med 26, no. 7 (April 2022): 2063–75. https://doi.org/10.1111/jcmm.17229.
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  - Bachman, William, Rupalatha Maddala, Ayon Chakraborty, Camelia Eldawy, Nikolai P. Skiba, and Ponugoti V. Rao. “Glucocorticoids Preferentially Influence Expression of Nucleoskeletal Actin Network and Cell Adhesive Proteins in Human Trabecular Meshwork Cells.” Front Cell Dev Biol 10 (2022): 886754. https://doi.org/10.3389/fcell.2022.886754.
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  - Karnam, Shruthi, Nikolai P. Skiba, and Ponugoti V. Rao. “Biochemical and biomechanical characteristics of dystrophin-deficient mdx3cv mouse lens.” Biochim Biophys Acta Mol Basis Dis 1867, no. 1 (January 1, 2021): 165998. https://doi.org/10.1016/j.bbadis.2020.165998.
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  - Skiba, Nikolai P., Martha A. Cady, Laurie Molday, John Y. S. Han, Tylor R. Lewis, William J. Spencer, Will J. Thompson, et al. “TMEM67, TMEM237, and Embigin in Complex With Monocarboxylate Transporter MCT1 Are Unique Components of the Photoreceptor Outer Segment Plasma Membrane.” Mol Cell Proteomics 20 (2021): 100088. https://doi.org/10.1016/j.mcpro.2021.100088.
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  - Kelly, Una L., Daniel Grigsby, Martha A. Cady, Michael Landowski, Nikolai P. Skiba, Jian Liu, Alan T. Remaley, Mikael Klingeborn, and Catherine Bowes Rickman. “High-density lipoproteins are a potential therapeutic target for age-related macular degeneration.” J Biol Chem 295, no. 39 (September 25, 2020): 13601–16. https://doi.org/10.1074/jbc.RA119.012305.
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  - Schmitt, Heather M., William M. Johnson, Inas F. Aboobakar, Shelby Strickland, María Gomez-Caraballo, Megan Parker, Laura Finnegan, et al. “Identification and activity of the functional complex between hnRNPL and the pseudoexfoliation syndrome-associated lncRNA, LOXL1-AS1.” Hum Mol Genet 29, no. 12 (July 29, 2020): 1986–95. https://doi.org/10.1093/hmg/ddaa021.
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  - Ray, Thomas A., Kelly Cochran, Chris Kozlowski, Jingjing Wang, Graham Alexander, Martha A. Cady, William J. Spencer, et al. “Comprehensive identification of mRNA isoforms reveals the diversity of neural cell-surface molecules with roles in retinal development and disease.” Nat Commun 11, no. 1 (July 3, 2020): 3328. https://doi.org/10.1038/s41467-020-17009-7.
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  - Spencer, William J., Tylor R. Lewis, Sebastien Phan, Martha A. Cady, Ekaterina O. Serebrovskaya, Nicholas F. Schneider, Keun-Young Kim, et al. “Photoreceptor disc membranes are formed through an Arp2/3-dependent lamellipodium-like mechanism.” Proc Natl Acad Sci U S A 116, no. 52 (December 26, 2019): 27043–52. https://doi.org/10.1073/pnas.1913518117.
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  - Klingeborn, Mikael, Nikolai P. Skiba, W Daniel Stamer, and Catherine Bowes Rickman. “Isolation of Retinal Exosome Biomarkers from Blood by Targeted Immunocapture.” Adv Exp Med Biol 1185 (2019): 21–25. https://doi.org/10.1007/978-3-030-27378-1_4.
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  - Klingeborn, Mikael, W Michael Dismuke, Nikolai P. Skiba, Una Kelly, W Daniel Stamer, and Catherine Bowes Rickman. “Author Correction: Directional Exosome Proteomes Reflect Polarity-Specific Functions in Retinal Pigmented Epithelium Monolayers.” Sci Rep 8, no. 1 (November 20, 2018): 17327. https://doi.org/10.1038/s41598-018-35542-w.
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  - Lobanova, Ekaterina S., Stella Finkelstein, Jing Li, Amanda M. Travis, Ying Hao, Mikael Klingeborn, Nikolai P. Skiba, Raymond J. Deshaies, and Vadim Y. Arshavsky. “Increased proteasomal activity supports photoreceptor survival in inherited retinal degeneration.” Nat Commun 9, no. 1 (April 30, 2018): 1738. https://doi.org/10.1038/s41467-018-04117-8.
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  - Ho, Leona T. Y., Nikolai Skiba, Christoph Ullmer, and Ponugoti Vasantha Rao. “Lysophosphatidic Acid Induces ECM Production via Activation of the Mechanosensitive YAP/TAZ Transcriptional Pathway in Trabecular Meshwork Cells.” Invest Ophthalmol Vis Sci 59, no. 5 (April 1, 2018): 1969–84. https://doi.org/10.1167/iovs.17-23702.
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  - Dexter, Paige M., Ekaterina S. Lobanova, Stella Finkelstein, William J. Spencer, Nikolai P. Skiba, and Vadim Y. Arshavsky. “Transducin β-Subunit Can Interact with Multiple G-Protein γ-Subunits to Enable Light Detection by Rod Photoreceptors.” Eneuro 5, no. 3 (2018). https://doi.org/10.1523/ENEURO.0144-18.2018.
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  - Maddala, Rupalatha, Nikolai P. Skiba, and Ponugoti Vasantha Rao. “Vertebrate Lonesome Kinase Regulated Extracellular Matrix Protein Phosphorylation, Cell Shape, and Adhesion in Trabecular Meshwork Cells.” J Cell Physiol 232, no. 9 (September 2017): 2447–60. https://doi.org/10.1002/jcp.25582.
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  - Klingeborn, Mikael, W Michael Dismuke, Nikolai P. Skiba, Una Kelly, W Daniel Stamer, and Catherine Bowes Rickman. “Directional Exosome Proteomes Reflect Polarity-Specific Functions in Retinal Pigmented Epithelium Monolayers.” Sci Rep 7, no. 1 (July 7, 2017): 4901. https://doi.org/10.1038/s41598-017-05102-9.
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  - Yang, Ping, Nikolai P. Skiba, Grace M. Tewkesbury, Victoria M. Treboschi, Peter Baciu, and Glenn J. Jaffe. “Complement-Mediated Regulation of Apolipoprotein E in Cultured Human RPE Cells.” Invest Ophthalmol Vis Sci 58, no. 7 (June 1, 2017): 3073–85. https://doi.org/10.1167/iovs.16-20083.
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  - Muralidharan, Arumugam Ramachandran, Rupalatha Maddala, Nikolai P. Skiba, and Ponugoti Vasantha Rao. “Growth Differentiation Factor-15-Induced Contractile Activity and Extracellular Matrix Production in Human Trabecular Meshwork Cells.” Invest Ophthalmol Vis Sci 57, no. 15 (December 1, 2016): 6482–95. https://doi.org/10.1167/iovs.16-20671.
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  - Spencer, William J., Jillian N. Pearring, Raquel Y. Salinas, David R. Loiselle, Nikolai P. Skiba, and Vadim Y. Arshavsky. “Progressive Rod-Cone Degeneration (PRCD) Protein Requires N-Terminal S-Acylation and Rhodopsin Binding for Photoreceptor Outer Segment Localization and Maintaining Intracellular Stability.” Biochemistry 55, no. 36 (September 13, 2016): 5028–37. https://doi.org/10.1021/acs.biochem.6b00489.
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  - Krasovskaya, L. A., N. V. Rudenko, O. P. Shuvalova, N. A. Sukharicheva, S. G. Abbasova, N. P. Skiba, and O. A. Stepnaya. “Optimization of in vivo crosslinking technique for the study of AlpB-protein interactions in Lysobacter sp. XL1 cells.” Process Biochemistry 48, no. 8 (August 1, 2013): 1203–7. https://doi.org/10.1016/j.procbio.2013.05.022.
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  - Lobanova, Ekaterina S., Stella Finkelstein, Nikolai P. Skiba, and Vadim Y. Arshavsky. “Proteasome overload is a common stress factor in multiple forms of inherited retinal degeneration.” Proc Natl Acad Sci U S A 110, no. 24 (June 11, 2013): 9986–91. https://doi.org/10.1073/pnas.1305521110.
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  - Skiba, Nikolai P., William J. Spencer, Raquel Y. Salinas, Eric C. Lieu, J Will Thompson, and Vadim Y. Arshavsky. “Proteomic identification of unique photoreceptor disc components reveals the presence of PRCD, a protein linked to retinal degeneration.” J Proteome Res 12, no. 6 (June 7, 2013): 3010–18. https://doi.org/10.1021/pr4003678.
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  - Maddala, Rupalatha, Nikolai P. Skiba, Robert Lalane, Diane L. Sherman, Peter J. Brophy, and Ponugoti V. Rao. “Periaxin is required for hexagonal geometry and membrane organization of mature lens fibers.” Dev Biol 357, no. 1 (September 1, 2011): 179–90. https://doi.org/10.1016/j.ydbio.2011.06.036.
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  - Reidel, Boris, J Will Thompson, Sina Farsiu, M Arthur Moseley, Nikolai P. Skiba, and Vadim Y. Arshavsky. “Proteomic profiling of a layered tissue reveals unique glycolytic specializations of photoreceptor cells.” Mol Cell Proteomics 10, no. 3 (March 2011): M110.002469. https://doi.org/10.1074/mcp.M110.002469.
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  - Inoue, Toshihiro, Paula Pecen, Rupalatha Maddala, Nikolai P. Skiba, Padmanabhan P. Pattabiraman, David L. Epstein, and P Vasantha Rao. “Characterization of cytoskeleton-enriched protein fraction of the trabecular meshwork and ciliary muscle cells.” Invest Ophthalmol Vis Sci 51, no. 12 (December 2010): 6461–71. https://doi.org/10.1167/iovs.10-5318.
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  - Lobanova, Ekaterina S., Rolf Herrmann, Stella Finkelstein, Boris Reidel, Nikolai P. Skiba, Wen-Tao Deng, Rebecca Jo, Ellen R. Weiss, William W. Hauswirth, and Vadim Y. Arshavsky. “Mechanistic basis for the failure of cone transducin to translocate: why cones are never blinded by light.” J Neurosci 30, no. 20 (May 19, 2010): 6815–24. https://doi.org/10.1523/JNEUROSCI.0613-10.2010.
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  - Zhang, Xiu-Jun, Nikolai P. Skiba, and Rick H. Cote. “Structural requirements of the photoreceptor phosphodiesterase gamma-subunit for inhibition of rod PDE6 holoenzyme and for its activation by transducin.” J Biol Chem 285, no. 7 (February 12, 2010): 4455–63. https://doi.org/10.1074/jbc.M109.057406.
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  - Kelly, Una, Catherine Bowes Rickman, Eric A. Postel, Michael A. Hauser, Gregory S. Hageman, Vadim Y. Arshavsky, and Nikolai P. Skiba. “Rapid and sensitive method for detection of Y402, H402, I62, and V62 variants of complement factor H in human plasma samples using mass spectrometry.” Invest Ophthalmol Vis Sci 50, no. 4 (April 2009): 1540–45. https://doi.org/10.1167/iovs.08-2782.
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  - Baker, Sheila A., Mohammad Haeri, Peter Yoo, Sidney M. Gospe, Nikolai P. Skiba, Barry E. Knox, and Vadim Y. Arshavsky. “The outer segment serves as a default destination for the trafficking of membrane proteins in photoreceptors.” J Cell Biol 183, no. 3 (November 3, 2008): 485–98. https://doi.org/10.1083/jcb.200806009.
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  - Rao, P Vasantha, Tammy Ho, Nikolai P. Skiba, and Rupalatha Maddala. “Characterization of lens fiber cell triton insoluble fraction reveals ERM (ezrin, radixin, moesin) proteins as major cytoskeletal-associated proteins.” Biochem Biophys Res Commun 368, no. 3 (April 11, 2008): 508–14. https://doi.org/10.1016/j.bbrc.2008.01.128.
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  - Chekanova, Julia A., Brian D. Gregory, Sergei V. Reverdatto, Huaming Chen, Ravi Kumar, Tanya Hooker, Junshi Yazaki, et al. “Genome-wide high-resolution mapping of exosome substrates reveals hidden features in the Arabidopsis transcriptome.” Cell 131, no. 7 (December 28, 2007): 1340–53. https://doi.org/10.1016/j.cell.2007.10.056.
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  - Maddala, Rupalatha, Nikolai Skiba, and Ponugoti Vasantha Rao. “Lens fiber cell elongation and differentiation is associated with a robust increase in myosin light chain phosphorylation in the developing mouse.” Differentiation 75, no. 8 (October 2007): 713–25. https://doi.org/10.1111/j.1432-0436.2007.00173.x.
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  - Lobanova, Ekaterina S., Stella Finkelstein, Hongman Song, Stephen H. Tsang, Ching-Kang Chen, Maxim Sokolov, Nikolai P. Skiba, and Vadim Y. Arshavsky. “Transducin translocation in rods is triggered by saturation of the GTPase-activating complex.” J Neurosci 27, no. 5 (January 31, 2007): 1151–60. https://doi.org/10.1523/JNEUROSCI.5010-06.2007.
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  - Martemyanov, Kirill A., Peter J. Yoo, Nikolai P. Skiba, and Vadim Y. Arshavsky. “R7BP, a novel neuronal protein interacting with RGS proteins of the R7 family.” J Biol Chem 280, no. 7 (February 18, 2005): 5133–36. https://doi.org/10.1074/jbc.C400596200.
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  - Chen, Ning-Fang, Jiang-Zhou Yu, Nikolai P. Skiba, Heidi E. Hamm, and Mark M. Rasenick. “A specific domain of Gialpha required for the transactivation of Gialpha by tubulin is implicated in the organization of cellular microtubules.” J Biol Chem 278, no. 17 (April 25, 2003): 15285–90. https://doi.org/10.1074/jbc.M300841200.
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  - Dell, Edward J., Jennifer Connor, Songhai Chen, Elizabeth G. Stebbins, Nikolai P. Skiba, Daria Mochly-Rosen, and Heidi E. Hamm. “The betagamma subunit of heterotrimeric G proteins interacts with RACK1 and two other WD repeat proteins.” J Biol Chem 277, no. 51 (December 20, 2002): 49888–95. https://doi.org/10.1074/jbc.M202755200.
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  - Dell, E. J., Trillium Blackmer, Nikolai P. Skiba, Yehia Daaka, Lee R. Shekter, Ramon Rosal, Eitan Reuveny, and Heidi E. Hamm. “Defining G protein beta gamma specificity for effector recognition.” Methods Enzymol 344 (2002): 421–34. https://doi.org/10.1016/s0076-6879(02)44731-3.
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  - Skiba, N. P., K. A. Martemyanov, A. Elfenbein, J. A. Hopp, A. Bohm, W. F. Simonds, and V. Y. Arshavsky. “RGS9-G beta 5 substrate selectivity in photoreceptors. Opposing effects of constituent domains yield high affinity of RGS interaction with the G protein-effector complex.” J Biol Chem 276, no. 40 (October 5, 2001): 37365–72. https://doi.org/10.1074/jbc.M106431200.
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  - Skiba, N. P., J. A. Hopp, and V. Y. Arshavsky. “The effector enzyme regulates the duration of G protein signaling in vertebrate photoreceptors by increasing the affinity between transducin and RGS protein.” J Biol Chem 275, no. 42 (October 20, 2000): 32716–20. https://doi.org/10.1074/jbc.C000413200.
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  - Savage, J. R., J. N. McLaughlin, N. P. Skiba, H. E. Hamm, and B. M. Willardson. “Functional roles of the two domains of phosducin and phosducin-like protein.” J Biol Chem 275, no. 39 (September 29, 2000): 30399–407. https://doi.org/10.1074/jbc.M005120200.
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  - Skiba, N. P., T. O. Thomas, and H. E. Hamm. “G alpha t/G alpha i1 chimeras used to define structural basis of specific functions of G alpha t.” Methods Enzymol 315 (2000): 502–24. https://doi.org/10.1016/s0076-6879(00)15864-1.
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  - Yang, C. S., N. P. Skiba, M. R. Mazzoni, T. O. Thomas, and H. E. Hamm. “Fluorescent probes as indicators of conformation changes in transducin on activation.” Methods Enzymol 315 (2000): 490–502. https://doi.org/10.1016/s0076-6879(00)15863-x.
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  - Skiba, N. P., C. S. Yang, T. Huang, H. Bae, and H. E. Hamm. “The alpha-helical domain of Galphat determines specific interaction with regulator of G protein signaling 9.” J Biol Chem 274, no. 13 (March 26, 1999): 8770–78. https://doi.org/10.1074/jbc.274.13.8770.
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  - Yang, C. S., N. P. Skiba, M. R. Mazzoni, and H. E. Hamm. “Conformational changes at the carboxyl terminus of Galpha occur during G protein activation.” J Biol Chem 274, no. 4 (January 22, 1999): 2379–85. https://doi.org/10.1074/jbc.274.4.2379.
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  - Ford, C. E., N. P. Skiba, H. Bae, Y. Daaka, E. Reuveny, L. R. Shekter, R. Rosal, et al. “Molecular basis for interactions of G protein betagamma subunits with effectors.” Science 280, no. 5367 (May 22, 1998): 1271–74. https://doi.org/10.1126/science.280.5367.1271.
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  - Bae, H., K. Anderson, L. A. Flood, N. P. Skiba, H. E. Hamm, and S. G. Graber. “Molecular determinants of selectivity in 5-hydroxytryptamine1B receptor-G protein interactions.” J Biol Chem 272, no. 51 (December 19, 1997): 32071–77. https://doi.org/10.1074/jbc.272.51.32071.
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  - Lambright, D. G., J. Sondek, A. Bohm, N. P. Skiba, H. E. Hamm, and P. B. Sigler. “The 2.0 A crystal structure of a heterotrimeric G protein.” Nature 379, no. 6563 (January 25, 1996): 311–19. https://doi.org/10.1038/379311a0.
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  - Skiba, N. P., H. Bae, and H. E. Hamm. “Mapping of effector binding sites of transducin alpha-subunit using G alpha t/G alpha i1 chimeras.” J Biol Chem 271, no. 1 (January 5, 1996): 413–24. https://doi.org/10.1074/jbc.271.1.413.
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  - Skiba, N. P., N. O. Artemyev, and H. E. Hamm. “The carboxyl terminus of the gamma-subunit of rod cGMP phosphodiesterase contains distinct sites of interaction with the enzyme catalytic subunits and the alpha-subunit of transducin.” J Biol Chem 270, no. 22 (June 2, 1995): 13210–15. https://doi.org/10.1074/jbc.270.22.13210.
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  - Arshavsky, V. Y., C. L. Dumke, Y. Zhu, N. O. Artemyev, N. P. Skiba, H. E. Hamm, and M. D. Bownds. “Regulation of transducin GTPase activity in bovine rod outer segments.” J Biol Chem 269, no. 31 (August 5, 1994): 19882–87.
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  - Rarick, H. M., N. O. Artemyev, J. S. Mills, N. P. Skiba, and H. E. Hamm. “Specific peptide probes for G-protein interaction with effectors.” Methods Enzymol 238 (1994): 13–28. https://doi.org/10.1016/0076-6879(94)38004-x.
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  - Artemyev, N. O., N. P. Skiba, J. S. Mills, and H. E. Hamm. “Rod cGMP-Phosphodiesterase γ-Subunit: Structure-Function Relationships.” Methods 5, no. 3 (January 1, 1993): 220–28. https://doi.org/10.1006/meth.1993.1027.
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  - Artemyev, N. O., H. M. Rarick, J. S. Mills, N. P. Skiba, and H. E. Hamm. “Sites of interaction between rod G-protein alpha-subunit and cGMP-phosphodiesterase gamma-subunit. Implications for the phosphodiesterase activation mechanism.” J Biol Chem 267, no. 35 (December 15, 1992): 25067–72.
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  - Lipkin, V. M., I. P. Udovichenko, V. A. Bondarenko, A. A. Yurovskaya, E. V. Telnykh, and N. P. Skiba. “Site-directed mutagenesis of the inhibitory subunit of retinal rod cyclic GMP phosphodiesterase.” Biomed Sci 1, no. 3 (March 1990): 305–8.
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  - Muradov, H. G., M. Y. Natochin, V. A. Bondarenko, N. P. Skiba, and V. M. Lipkin. “Interactions between bovine retinal rod c GMP phosphodiesterase subunits.” Biological Membranes 7, no. 6 (1990): 565–72.
  - Skiba, N. P., I. P. Udovichenko, V. A. Bondarenko, M. Y. Natochin, and A. A. Yurovskaya. “Expression of the gamma subunit of the cyclic GMP phosphodiesterase gene from bovine rod outer segments in Escherichia coli.” Biological Membranes 7, no. 3 (1990): 565–72.
  - Efimov, V. A., I. V. Aleksiuk, A. A. Buriakova, I. N. Pashkova, N. P. Skiba, and O. G. Chakhmakhcheva. “[Synthesis of human proinsulin in Escherichia coli cells].” Bioorg Khim 15, no. 8 (August 1989): 1078–90.
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  - Lipkin, V. M., O. N. Shamborant, V. V. Gubanov, K. G. Muradov, I. P. Udovichenko, V. A. Bondarenko, V. A. Natochin MYu, and N. P. Skiba. “Structural and functional studies of cyclic GMP phosphodiesterase.” J Protein Chem 8, no. 3 (June 1989): 406–8. https://doi.org/10.1007/BF01674303.
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  - Skiba, N. P., V. A. Efimov, V. M. Lipkin, O. G. Chakhmakhcheva, and Iu A. Ovchinnikov. “[Localization of binding sites of E. coli DNA-dependent RNA-polymerase with photosensitive template analogs].” Bioorg Khim 12, no. 8 (August 1986): 1023–29.
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  - Ovchinnikov, Y. A., V. A. Efimov, I. N. Ivanova, S. V. Reverdatto, N. P. Skiba, and O. G. Chakhmakhcheva. “Synthesis of DNA coding for human proinsulin.” Gene 31, no. 1–3 (November 1984): 65–78. https://doi.org/10.1016/0378-1119(84)90196-3.
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  - Ovchinnikov, Y. A., V. A. Efimov, O. G. Chakhmakhcheva, N. P. Skiba, and V. M. Lipkin. “INTERACTION OF DNA-DEPENDENT RNA-POLYMERASE ESCHERICHIA-COLI WITH ANALOGS OF THE PROMOTER REGION OF BACTERIOPHAGE FD-DNA CONTAINING 5-BROMOURACIL RESIDUES IN THE TEMPLATE STRAND.” Bioorganicheskaya Khimiya 7, no. 4 (1981): 485–485.
  - Ovchinnikov, Y. A., V. A. Efimov, O. G. Chakhmakhcheva, N. P. Skiba, V. M. Lipkin, and N. N. Modyanov. “COVALENT CROSS-LINKING OF ESCHERICHIA-COLI RNA-POLYMERASE AND PHOTOSENSITIVE ANALOGS OF DECATHYMIDYLIC ACID.” Bioorganicheskaya Khimiya 5, no. 9 (1979): 1410–20.
- Other Articles
  - Graber, S. G., H. Bae, K. M. Deprec, J. E. Slessareva, N. P. Skiba, and H. E. Harnm. “Molecular determinants of g protein signaling selectivity.” Faseb Journal, December 1, 1998.
  - Skiba, N. P., and H. E. Hamm. “How Gsalpha activates adenylyl cyclase.” Nat Struct Biol, February 1998. https://doi.org/10.1038/nsb0298-88.
    Full Text Link to Item
- Conference Papers
  - Bachman, William, Rupalatha Maddala, Camelia Eldawy, Nikolai P. Skiba, and Vasantha Rao. “Role of Brg1/SMARCA4 chromatin remodeling protein in the expression of genes involved in actin cytoskeletal organization and cell adhesion in the trabecular meshwork cells.” In Investigative Ophthalmology & Visual Science, Vol. 63, 2022.
    Link to Item
  - Gorijavolu, Pallavi, Rupalatha Maddala, William Bachman, Nikolai P. Skiba, Rakesh K. Singh, and Vasantha Rao. “Role of Septins in Regulation of Actin Cytoskeletal Organization in Trabecular Meshwork Cells.” In Investigative Ophthalmology & Visual Science, Vol. 63, 2022.
    Link to Item
  - Maddala, Rupalatha, William Backman, Camelia Eldawy, Nikolai P. Skiba, and Vasantha Rao. “Novel molecular mechanisms regulating actin cytoskeletal reorganization in dexamethasone treated trabecular meshwork cells.” In Investigative Ophthalmology & Visual Science, Vol. 63, 2022.
    Link to Item
  - McDonnell, Fiona S., Bre’Ida J. Riddick, Haven Roberts, Nikolai P. Skiba, and Daniel W. Stamer. “Trabecular meshwork exosomes and the extracellular matrix.” In Investigative Ophthalmology & Visual Science, Vol. 63, 2022.
    Link to Item
  - Rao, Vasantha, Camelia Eldawy, William Backman, Nikolai P. Skiba, and Rupalatha Maddala. “Activation of Glypican-4 regulated Wnt5/PCP pathway in dexamethasone treated trabecular meshwork cells.” In Investigative Ophthalmology & Visual Science, Vol. 63, 2022.
    Link to Item
  - Skiba, Nikolai P., Tylor Lewis, William J. Spencer, Carson Castillo, Andrej Shevchenko, and Vadim Y. Arshavsky. “Absolute quantification of phototransduction and other rod outer segment proteins using a novel technique of MS-Western.” In Investigative Ophthalmology & Visual Science, Vol. 63, 2022.
    Link to Item
  - Spencer, William J., Nicholas F. Schneider, Nikolai P. Skiba, and Vadim Y. Arshavsky. “The WAVE complex is an essential component of the actin network that initiates photoreceptor disc membrane morphogenesis.” In Investigative Ophthalmology & Visual Science, Vol. 63, 2022.
    Link to Item
  - Spencer, William, Jillian Nydam Pearring, Jindong Ding, Nikolai P. Skiba, Marie E. Burns, and Vadim Y. Arshavsky. “PRCD supports the organized structure of the photoreceptor outer segment.” In Investigative Ophthalmology & Visual Science, Vol. 60. ASSOC RESEARCH VISION OPHTHALMOLOGY INC, 2019.
    Link to Item
  - Skiba, Nikolai P., Laurie Molday, Robert S. Molday, and Vadim Y. Arshavsky. “Identification of protein components of the rod outer segment plasma membrane by label-free protein correlation profiling.” In Investigative Ophthalmology & Visual Science, Vol. 59. ASSOC RESEARCH VISION OPHTHALMOLOGY INC, 2018.
    Link to Item
  - Yang, Ping, Nikolai P. Skiba, Grace M. Tewkesbury, Peter Baciu, and Glenn J. Jaffe. “Complement-mediated Apolipoprotein E Accumulation in Cultured Human RPE Cell.” In Investigative Ophthalmology & Visual Science, Vol. 56. ASSOC RESEARCH VISION OPHTHALMOLOGY INC, 2015.
    Link to Item
  - Sokolov, Maxim, Marycharmain Belcastro, Xueli Gao, Vadim Y. Arshavsky, Nikolai P. Skiba, and Satyabrata Sinha. “Studies of Protein Interaction of the Chaperonin CCT in Photoreceptors.” In Investigative Ophthalmology & Visual Science, Vol. 55. ASSOC RESEARCH VISION OPHTHALMOLOGY INC, 2014.
    Link to Item
  - Yang, Ping, Nikolai P. Skiba, Michelle Bao, Angel Long, Peter Baciu, and Glenn J. Jaffe. “Proteomics Analysis of Protein Expression in Human RPE Cells Exposed to Oxidant and Complement Attack through Alternative Pathway.” In Investigative Ophthalmology & Visual Science, Vol. 55. ASSOC RESEARCH VISION OPHTHALMOLOGY INC, 2014.
    Link to Item
  - Skiba, N. P., J. A. Hopp, E. R. Makino, A. Bohm, W. F. Simonds, and V. Y. Arshavsky. “The role of G protein beta 5 subunit in regulating transducin GTPase.” In Investigative Ophthalmology & Visual Science, 41:S322–S322. ASSOC RESEARCH VISION OPHTHALMOLOGY INC, 2000.
    Link to Item
  - Ford, C. E., H. Bae, N. P. Skiba, and H. E. Hamm. “Molecular basis for Gβy-dependent interactions.” In Faseb Journal, Vol. 11, 1997.
  - Skiba, N., H. Bae, C. Ford, Y. Daaka, E. Reuvenv, L. Shekter, T. Van Biesen, et al. “Molecular basis for gβγ-effector interaction: Structural correlation with ga binding site.” In Faseb Journal, Vol. 11, 1997.
  - Bae, H., K. Anderson, L. A. Flood, N. P. Skiba, H. E. Hamm, and S. G. Gräber. “Molecular Determinants of Selectivity in Receptor-G protein Interaction.” In Faseb Journal, Vol. 11, 1997.

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