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Nikolai Petrovich Skiba

Nikolai Petrovich Skiba

Associate Professor of Ophthalmology
Ophthalmology
Box 3802 Med Ctr, Durham, NC 27710
Wadsworth Bldg, Durham, NC 27710

Overview

My research focuses on applying mass spectrometry based proteomics to study proteins in eye tissues, cells and sub-cellular compartments to understand mechanisms of vision. An important aspect of my research is to identify proteins in different compartments of retinal photoreceptor cells, their amount and modification status at different cell states defined by the light conditions, genotype, disease etc. This information can be valuable in understanding molecular mechanisms of vision and biology of the photoreceptor cell. Another important aspect of my research is to assist basic scientist and clinicians in our department in their proteomic needs which include identification of proteins and other biomolecules in a given biological sample, detection of protein post-translational modifications and sequence variations, elucidation of protein-protein interactions and also characterization of changes in the protein concentration and composition in a biological sample at different conditions.

Current Appointments & Affiliations

Associate Professor of Ophthalmology · 2021 - Present Ophthalmology, Clinical Science Departments

Recent Publications

Physiological activation of liver X receptor provides protection against ocular inflammation in uveitic glaucoma.

Journal Article Biochimica et biophysica acta. Molecular basis of disease · January 2025 Virus-induced trabeculitis is considered a significant cause of uveitic glaucoma, being marked by a sudden increase in intraocular pressure and relatively mild inflammation in the anterior chamber of the eye. In previous proteome analyses of aqueous humor ... Full text Cite

Ankyrin-B is required for the establishment and maintenance of lens cytoarchitecture, mechanics and clarity.

Journal Article J Cell Sci · December 15, 2024 The transparent ocular lens is essential for vision because it focuses light onto the retina. Despite recognition of the importance of its unique cellular architecture and mechanical properties, the molecular mechanisms governing these attributes remain el ... Full text Link to item Cite

Dysregulation of septin cytoskeletal organization in the trabecular meshwork contributes to ocular hypertension.

Journal Article JCI Insight · December 6, 2024 Ocular hypertension, believed to result partly from increased contractile activity, cell adhesive interactions, and stiffness within the trabecular meshwork (TM), is a major risk factor for glaucoma, a leading cause of blindness. However, the identity of m ... Full text Link to item Cite
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Recent Grants

Identification of proteins localized to photoreceptor connecting cilium

ResearchPrincipal Investigator · Awarded by National Institutes of Health · 2024 - 2026

Identification of novel contributors to retinitis pigmentosa using metabolic and proteomic approaches

ResearchPrincipal Investigator · Awarded by University of North Carolina - Chapel Hill · 2021 - 2024

Deciphering the local effect of glaucoma risk factors on axonal mitoproteome

ResearchCollaborator · Awarded by BrightFocus Foundation · 2021 - 2024

View All Grants

Education, Training & Certifications

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry (Russia) · 1986 Ph.D.
Lomonosov Moscow State Universty (Russia) · 1978 B.S.